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Man2b1 (NM_010764) Mouse Tagged ORF Clone
CAT＃: MG211444
- TrueORF®
Man2b1 (tGFP-tagged) - Mouse mannosidase 2, alpha B1 (Man2b1)
ORF Plasmid: DDK
"NM_010764" in other vectors (4)
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CNY 9,690.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AW107687; LAMAN |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG211444 representing NM_010764
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCACCGGACCGCTCACCTCTGGTGTCCGTGCTGGTGGCGGGAACACCGGGTGGCTCTGGATGAGCT CCTGCAACCTAGGTTCGCCAGTCCTGCCTATTTCCTTTCTTTTTTGGCTGTTGCTGGCCGCGCCTGGCGC TCGGGCCGCGGGTTACAAGACATGTCCCCCAACGAAGCCAGGCATGCTCAACGTGCACCTGCTGCCTCAT ACACACGATGATGTAGGCTGGCTGAAGACAGTGGACCAATACTATTACGGCATCCTGAGTGATGTTCAAC ACGCGTCTGTTCAGTACATCCTAGACTCAGTAGTCTCCTCTCTACTGGAGAAACCCACCCGTCGCTTCAT CTATGTGGAAATGGCTTTCTTCTCCCGTTGGTGGAAGCAGCAGACCAGTGCAACCCAGGACGCTGTACGG AACCTGGTGCGCCAGGGGCGCCTGGAGTTTGTCAATGGTGGCTGGGTGATGAACGACGAAGCAGCCACCC ACTATGGCGCGATTGTGGACCAGATGACACTTGGGCTACGCTTCCTGCAAGACACATTTGGTAGTGATGG ACTTCCCCGTGTGGCCTGGCATATTGACCCTTTCGGCCACTCTCGAGAACAGGCCTCCCTGTTTGCCCAG ATGGGTTTTGATGGCTTCTTCCTTGGGCGCATTGACTATCAAGATAAACTTAATCGGAAGAAAAAGCTGA GGATGGAGGAGCTGTGGAGAGCTAGTGACAGCTTGGAGCCCCCCGCTGCTGACCTCTTTACTGGTGTGCT CCCCAATAATTACAACCCCCCGAAATACCTGTGCTGGGATGTGCTATGTACAGACCCTCCCGTGGTGGAT AATCCCCGGAGCCCTGAGTTCAATGCAAAAACGTTGGTCAACTACTTCCTGAAGCTCGCTTCTTCCCAGA AAGGGTTTTACCGAACCAACCACACCGTGATGACCATGGGCTCAGACTTCCATTATGAGAATGCCAACAT GTGGTTCAAAAACATGGACAAGCTGATCCGCCTGGTCAACGCGCAGCAGGCAAACGGGAGCCTCGTCCAC GTTCTCTACTCCACCCCTACGTGTTACCTCTGGGAGCTGAACAAGGCCAATCTCACCTGGACAGTGAAGG AGGACGACTTCTTTCCTTATGCCGATGGCCCCCACATGTTCTGGACTGGCTATTTTTCCAGCAGGCCAGC CCTCAAGCGCTATGAGCGCCTCAGTTACAACTTCCTGCAGGTGTGTAACCAGCTGGAAGCACTGGTGGGT CCCGAAGCCAACGTGGGACCCTATGGCTCGGGAGACAGCGCACCTCTTCAGGAGGCAATGGCGGTGCTCC AACACCACGACGCTGTCAGTGGTACTGCAAGGCAGAATGTTGTAAATGACTACGCGAGGCAGCTGGCAGC AGGCTGGGGACCCTGTGAGGTTCTCGTGAGCAACGCTCTGGCGCGACTCAGCCATTACAAACAGAACTTC TCATTCTGTCGTGAGCTCAACATTAGCATCTGCCCAGTCAGCCAGACTTCGGAGCGTTTCCAGGTAACTC TTTACAACCCCCTGGGGCGAAAAGTGGATCAGATGGTTCGGCTGCCTGTCTATGAAGGCAACTTCATTGT GAAGGACCCTCATGATAAGAACATTTCCAGTAATGTGGTGATGGTTCCTAGCTATTACAGTGAGACTTAC CAATGGGAGCTGCTGTTCCCAGCCTCAGTGCCTGCCCTGGGCTTCAGCACCTACTCTGTGGCCAAAATGA GTGACCTTAACCACCAAGCCCACAACCTGCTGTCCAGGCCCAGGAAACACAAGTCCCATCATGTCTTAGT CATTGAAAATAAGTACATGCGGGCCACATTTGACTCTGGCACGGGGTTATTGATGAAGATTGAAAACTTG GAACAGAACCTCTCGCTCCCTGTGAGCCAAGGCTTCTTTTGGTATAATGCCAGTGTTGGTGATGAGGAGA GTTCTCAGGCCTCTGGGGCCTACATCTTCAGACCCAATGTCGGTAAACCGATACCTGTGAGCCGCTGGGC TCAGATCAGTTTGGTGAAGACAGCCTTGGTGCAGGAGGTACACCAGAATTTTTCAGCCTGGTGTTCCCAA GTGATTCGCCTGTACAAAGGACAGCGCCATTTGGAGCTGGAGTGGACGGTGGGACCAATCCCAGTGAGAG ATGACTGGGGGAAGGAAGTCATCAGCCGCTTTGACACCCCAATGAAAACCAAGGGACAGTTCTTCACGGA CAGCAACGGCCGGGAAATCCTGAAGAGGAGGGATGATTATCGACCCACCTGGACATTGAATCAGACTGAG CCGGTGGCTGGAAATTACTATCCTGTCAACACTCGAATTTACATCACGGATGGGCAAATGCAGCTGACCG TGTTGACCGACCGCTCCCAGGGGGGCAGCAGTCTGCAGGATGGCTCGTTGGAGCTCATGGTGCACCGTCG GCTGTTGGTAGATGATGATCGTGGAGTGTCGGAACCCTTGTTGGAGACAGATACCGGGGATAAGGTTCGA GGACGCCACCTTGTGCTTTTGAGTTCTGTCAGTGATGCCGCTGCAAGACACCGACTGCTGGCGGAACAAG AAGTCCTGGCCCCTCAGGTGGTGCTGTCTCTGGGTGGTAGCAGTCCCTATCACTCCCGGGCGACACCAAA AACGCAGTTTTCCGGACTTCGCCAGGAGCTACCTCCTCAGGTGCATTTGCTCACACTGGCCCGCTGGGGG CCAAAGATGCTGCTGCTGCGCTTGGAGCACCAGTTCGCTCTGAAAGAAGATTCGGATCGCAACCTGAGCT CCCCTGTAACCTTGAACGTTCAGAACCTGTTCCAGACCTTCACTATCAACTACCTGCAGGAGACCACACT GGCAGCCAACCAGCCCCTGTCCAGGGCTTCCAGGCTCAAGTGGATGACAAATACTGGTCCCACCTCCTTC CCTGAGCCTTCCAAGTTGGACCCTACCTCAGTCACACTGAAGCCTATGGAAATACGTACCTTCCTGGCCT CTGTACAATGGCAAGAACACAGGCCTGCC ACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG211444 representing NM_010764
Red=Cloning site Green=Tags(s) MGTGPLTSGVRAGGGNTGWLWMSSCNLGSPVLPISFLFWLLLAAPGARAAGYKTCPPTKPGMLNVHLLPH THDDVGWLKTVDQYYYGILSDVQHASVQYILDSVVSSLLEKPTRRFIYVEMAFFSRWWKQQTSATQDAVR NLVRQGRLEFVNGGWVMNDEAATHYGAIVDQMTLGLRFLQDTFGSDGLPRVAWHIDPFGHSREQASLFAQ MGFDGFFLGRIDYQDKLNRKKKLRMEELWRASDSLEPPAADLFTGVLPNNYNPPKYLCWDVLCTDPPVVD NPRSPEFNAKTLVNYFLKLASSQKGFYRTNHTVMTMGSDFHYENANMWFKNMDKLIRLVNAQQANGSLVH VLYSTPTCYLWELNKANLTWTVKEDDFFPYADGPHMFWTGYFSSRPALKRYERLSYNFLQVCNQLEALVG PEANVGPYGSGDSAPLQEAMAVLQHHDAVSGTARQNVVNDYARQLAAGWGPCEVLVSNALARLSHYKQNF SFCRELNISICPVSQTSERFQVTLYNPLGRKVDQMVRLPVYEGNFIVKDPHDKNISSNVVMVPSYYSETY QWELLFPASVPALGFSTYSVAKMSDLNHQAHNLLSRPRKHKSHHVLVIENKYMRATFDSGTGLLMKIENL EQNLSLPVSQGFFWYNASVGDEESSQASGAYIFRPNVGKPIPVSRWAQISLVKTALVQEVHQNFSAWCSQ VIRLYKGQRHLELEWTVGPIPVRDDWGKEVISRFDTPMKTKGQFFTDSNGREILKRRDDYRPTWTLNQTE PVAGNYYPVNTRIYITDGQMQLTVLTDRSQGGSSLQDGSLELMVHRRLLVDDDRGVSEPLLETDTGDKVR GRHLVLLSSVSDAAARHRLLAEQEVLAPQVVLSLGGSSPYHSRATPKTQFSGLRQELPPQVHLLTLARWG PKMLLLRLEHQFALKEDSDRNLSSPVTLNVQNLFQTFTINYLQETTLAANQPLSRASRLKWMTNTGPTSF PEPSKLDPTSVTLKPMEIRTFLASVQWQEHRPA TRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-NotI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_010764 |
| ORF Size | 3039 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_010764.2, NP_034894.2 |
| RefSeq Size | 4326 bp |
| RefSeq ORF | 3042 bp |
| Locus ID | 17159 |
| UniProt ID | O09159 |
| Gene Summary | Necessary for the catabolism of N-linked carbohydrates released during glycoprotein turnover.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223199 | Man2b1 (untagged) - Mouse mannosidase 2, alpha B1 (Man2b1), (10ug) |
CNY 13,110.00 |
|
| MR211444 | Man2b1 (Myc-DDK-tagged) - Mouse mannosidase 2, alpha B1 (Man2b1) |
CNY 7,064.00 |
|
| MR211444L3 | Lenti ORF clone of Man2b1 (Myc-DDK-tagged) - Mouse mannosidase 2, alpha B1 (Man2b1) |
CNY 10,740.00 |
|
| MR211444L4 | Lenti ORF clone of Man2b1 (mGFP-tagged) - Mouse mannosidase 2, alpha B1 (Man2b1) |
CNY 10,740.00 |
