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Atp1a1 (NM_144900) Mouse Tagged ORF Clone
CAT＃: MG211466
- TrueORF®
Atp1a1 (tGFP-tagged) - Mouse ATPase, Na+/K+ transporting, alpha 1 polypeptide (Atp1a1)
ORF Plasmid: DDK
"NM_144900" in other vectors (4)
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CNY 11,310.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Atpa-1; BC010319 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG211466 representing NM_144900
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGAAGGGGGTTGGACGAGACAAGTATGAGCCTGCCGCTGTATCGGAGCATGGTGACAAAAAGGGCA AAAAGGCCAAGAAGGAAAGGGATATGGATGAGCTCAAGAAGGAAGTGTCTATGGACGACCATAAACTCAG CCTGGATGAACTCCATCGTAAATACGGAACAGATTTGAGCCGAGGATTAACACCTGCAAGGGCCGCTGAG ATCCTGGCCCGGGATGGCCCCAACGCCCTCACACCCCCTCCCACTACTCCCGAATGGGTGAAATTCTGCC GGCAGCTCTTTGGAGGCTTTTCCATGTTACTGTGGATCGGGGCCATTCTTTGTTTCCTGGCTTATGGCAT CCGAAGTGCTACAGAAGAGGAACCGCCAAACGATGATCTGTACCTCGGGGTGGTGCTCTCTGCTGTAGTC ATCATAACTGGCTGTTTCTCCTATTATCAAGAAGCAAAAAGCTCCAAGATCATGGAATCCTTCAAGAACA TGGTCCCTCAGCAAGCCCTTGTGATTCGAAATGGAGAGAAAATGAGCATCAATGCGGAGGATGTCGTCGT CGGGGATCTGGTGGAGGTGAAAGGGGGAGACCGGATCCCTGCTGATCTTAGAATCATATCTGCAAACGGC TGCAAGGTGGATAACTCCTCACTCACTGGTGAATCAGAACCCCAGACTCGGTCCCCGGATTTCACAAACG AGAACCCCTTGGAGACAAGGAACATTGCCTTCTTCTCAACCAACTGTGTGGAAGGAACCGCACGTGGCAT CGTCGTGTACACTGGGGACCGCACCGTGATGGGCAGGATTGCCACGCTTGCCTCTGGGCTAGAAGGTGGC CAGACCCCCATTGCTGAAGAGATTGAGCATTTCATCCACCTCATCACCGGGGTGGCCGTGTTCCTGGGGG TGTCTTTCTTCATCCTTTCTCTGATCCTTGAGTACACCTGGCTCGAGGCTGTCATCTTCCTCATTGGTAT CATCGTAGCCAACGTGCCAGAAGGTCTGCTGGCCACTGTCACGGTCTGTCTGACACTCACTGCCAAGCGC ATGGCGAGGAAGAACTGCCTGGTGAAGAACTTGGAAGCTGTGGAGACCTTGGGGTCCACATCTACCATCT GCTCAGACAAGACGGGAACTCTGACTCAGAACCGGATGACAGTGGCCCACATGTGGTTTGACAATCAGAT CCATGAAGCTGACACCACGGAGAATCAGAGTGGTGTCTCCTTTGACAAGACGTCAGCCACCTGGTTCGCT CTGTCCAGAATTGCTGGTCTCTGTAACAGGGCAGTGTTTCAGGCTAACCAAGAAAACCTGCCTATTCTTA AGCGGGCAGTAGCGGGAGATGCCTCTGAGTCGGCGCTCTTAAAGTGCATTGAAGTCTGCTGCGGCTCCGT GATGGAGATGAGGGAGAAATACAGCAAGATAGTGGAGATTCCCTTCAACTCCACCAACAAGTACCAGTTG TCCATTCACAAGAACCCAAATGCATCTGAGCCCAAACACCTGCTAGTGATGAAGGGCGCCCCAGAAAGGA TCCTGGACCGCTGCAGCTCCATCCTTCTCCACGGCAAGGAGCAGCCACTGGATGAAGAGCTGAAGGACGC TTTTCAGAACGCCTACCTAGAGCTGGGGGGCCTTGGAGAACGTGTGCTAGGTTTCTGCCACCTCCTCCTG CCTGACGAACAGTTTCCCGAAGGCTTCCAGTTTGACACTGACGATGTCAATTTCCCCGTGGATAACCTCT GCTTCGTGGGTCTTATCTCCATGATTGACCCTCCCCGAGCTGCTGTCCCCGATGCTGTGGGCAAATGCCG CAGCGCTGGGATTAAGGTCATCATGGTTACAGGAGACCATCCAATCACAGCGAAAGCCATTGCCAAGGGG GTAGGCATTATCTCAGAAGGCAATGAGACTGTGGAAGACATTGCTGCCCGCCTCAACATTCCAGTGAACC AGGTGAACCCCAGAGATGCCAAGGCCTGTGTGGTCCACGGCAGTGACCTGAAGGACATGACCTCCGAGGA GCTGGATGACATCTTGCGGTACCACACAGAGATCGTCTTTGCCAGGACCTCTCCTCAGCAGAAGCTCATC ATTGTGGAGGGCTGCCAGCGGCAGGGCGCCATCGTGGCCGTCACAGGGGATGGTGTCAATGACTCTCCAG CTTTGAAAAAGGCAGATATTGGGGTTGCCATGGGGATTGTTGGCTCTGATGTGTCCAAGCAAGCTGCTGA CATGATCCTTCTGGATGACAACTTTGCCTCCATTGTGACTGGAGTAGAGGAAGGTCGTCTGATTTTTGAT AACTTGAAGAAATCCATCGCTTACACCCTAACCAGTAACATTCCGGAAATCACCCCCTTCTTGATATTTA TTATTGCAAACATTCCACTGCCCCTGGGGACTGTGACCATCCTCTGCATTGACTTGGGCACTGACATGGT TCCTGCCATCTCCCTGGCCTACGAGCAAGCTGAGAGCGACATCATGAAGAGGCAGCCCAGAAACCCCAAA ACGGACAAACTTGTGAACGAGCGTCTGATCAGCATGGCCTATGGACAGATCGGTATGATCCAGGCCCTGG GAGGCTTCTTCACTTACTTTGTGATTCTGGCTGAGAACGGTTTCCTGCCCTTTCACCTGTTGGGCATCCG AGAGACCTGGGATGACCGCTGGGTCAACGATGTGGAGGACAGCTACGGGCAGCAGTGGACCTACGAGCAG AGGAAGATCGTGGAGTTCACCTGCCATACAGCGTTCTTTGTCAGTATTGTGGTAGTGCAGTGGGCCGACT TGGTCATCTGCAAGACCAGAAGGAATTCTGTCTTCCAGCAGGGAATGAAGAACAAGATCTTGATATTTGG CCTCTTTGAAGAGACAGCCCTTGCTGCTTTCTTATCCTACTGCCCCGGGATGGGGGCAGCCCTTAGGATG TATCCCCTCAAACCTACATGGTGGTTCTGTGCCTTCCCCTACTCCCTTCTCATCTTTGTGTATGACGAGG TGCGGAAGCTCATTATCAGGCGGCGCCCTGGCGGCTGGGTGGAGAAGGAAACCTACTAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG211466 representing NM_144900
Red=Cloning site Green=Tags(s) MGKGVGRDKYEPAAVSEHGDKKGKKAKKERDMDELKKEVSMDDHKLSLDELHRKYGTDLSRGLTPARAAE ILARDGPNALTPPPTTPEWVKFCRQLFGGFSMLLWIGAILCFLAYGIRSATEEEPPNDDLYLGVVLSAVV IITGCFSYYQEAKSSKIMESFKNMVPQQALVIRNGEKMSINAEDVVVGDLVEVKGGDRIPADLRIISANG CKVDNSSLTGESEPQTRSPDFTNENPLETRNIAFFSTNCVEGTARGIVVYTGDRTVMGRIATLASGLEGG QTPIAEEIEHFIHLITGVAVFLGVSFFILSLILEYTWLEAVIFLIGIIVANVPEGLLATVTVCLTLTAKR MARKNCLVKNLEAVETLGSTSTICSDKTGTLTQNRMTVAHMWFDNQIHEADTTENQSGVSFDKTSATWFA LSRIAGLCNRAVFQANQENLPILKRAVAGDASESALLKCIEVCCGSVMEMREKYSKIVEIPFNSTNKYQL SIHKNPNASEPKHLLVMKGAPERILDRCSSILLHGKEQPLDEELKDAFQNAYLELGGLGERVLGFCHLLL PDEQFPEGFQFDTDDVNFPVDNLCFVGLISMIDPPRAAVPDAVGKCRSAGIKVIMVTGDHPITAKAIAKG VGIISEGNETVEDIAARLNIPVNQVNPRDAKACVVHGSDLKDMTSEELDDILRYHTEIVFARTSPQQKLI IVEGCQRQGAIVAVTGDGVNDSPALKKADIGVAMGIVGSDVSKQAADMILLDDNFASIVTGVEEGRLIFD NLKKSIAYTLTSNIPEITPFLIFIIANIPLPLGTVTILCIDLGTDMVPAISLAYEQAESDIMKRQPRNPK TDKLVNERLISMAYGQIGMIQALGGFFTYFVILAENGFLPFHLLGIRETWDDRWVNDVEDSYGQQWTYEQ RKIVEFTCHTAFFVSIVVVQWADLVICKTRRNSVFQQGMKNKILIFGLFEETALAAFLSYCPGMGAALRM YPLKPTWWFCAFPYSLLIFVYDEVRKLIIRRRPGGWVEKETYY TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_144900 |
| ORF Size | 3069 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_144900.2 |
| RefSeq Size | 3686 bp |
| RefSeq ORF | 3072 bp |
| Locus ID | 11928 |
| UniProt ID | Q8VDN2 |
| Gene Summary | This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC204215 | Atp1a1 (untagged) - Mouse ATPase, Na+/K+ transporting, alpha 1 polypeptide (Atp1a1), (10ug) |
CNY 7,128.00 |
|
| MR211466 | Atp1a1 (Myc-DDK-tagged) - Mouse ATPase, Na+/K+ transporting, alpha 1 polypeptide (Atp1a1) |
CNY 10,260.00 |
|
| MR211466L3 | Lenti ORF clone of Atp1a1 (Myc-DDK-tagged) - Mouse ATPase, Na+/K+ transporting, alpha 1 polypeptide (Atp1a1) |
CNY 12,160.00 |
|
| MR211466L4 | Lenti ORF clone of Atp1a1 (mGFP-tagged) - Mouse ATPase, Na+/K+ transporting, alpha 1 polypeptide (Atp1a1) |
CNY 12,160.00 |
