Mms19 (NM_028152) Mouse Tagged ORF Clone
CAT#: MG211485
- TrueORF®
Mms19 (tGFP-tagged) - Mouse MMS19 (MET18 S. cerevisiae)-like (Mms19l)
ORF Plasmid: DDK
"NM_028152" in other vectors (4)
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CNY 9,220.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | 2410001K24Rik; 2610042O15Rik; AI316855; C79368; C86341; Mms19l |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG211485 representing NM_028152
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGCCGCCACCGGTCTGGAGGAGGCAGTGGCGCCTATGGGCGCCCTGTGTGGCCTCGTGCAAGACT TCGTCATGGGTCAACAGGAGGGGCCCGCTGACCAGGTGGCTGCAGATGTGAAGTCCGGTGGCTATACAGT GTTACAAGTAGTAGAAGCCCTTGGGTCCTCTCTGGAAAATGCTGAACCCCGAACTCGGGCTCGAGGAGCC CAGCTTCTGTCACAGGTGCTGCTCCAGTGTCACTCCTTGCTCTCGGAGAAGGAAGTGGTCCACCTGATCC TGTTCTATGAGAACCGGCTGAAGGACCACCATCTTGTGGTCCCATCTGTCTTACAGGGCCTGAGGGCACT GAGCATGTCTGTGGCCCTGCCTCCGGGTCTGGCTGTCTCTGTGCTTAAAGCCATCTTCCAGGAGGTACAT GTACAGTCCCTGCTACAGGTGGACCGCCATACTGTCTTCAGCATCATCACTAACTTCATGCGATCACGAG AAGAAGAGCTGAAGGGTCTGGGAGCTGACTTCACATTTGGCTTCATCCAGGTGATGGATGGGGAGAAGGA TCCCCGTAATCTCCTGCTGGCTTTCCGCATCGTCCATGACCTCATCTCCAAGGACTACAGTCTGGGACCT TTCGTGGAAGAGTTATTTGAAGTGACATCTTGTTATTTCCCTATTGATTTTACCCCTCCGCCTAACGATC CTTATGGCATCCAGAGAGAGGATCTCATCCTGAGTCTCCGTGCTGTGCTGGCGTCTACACCGCGCTTTGC CGAGTTCTTACTGCCTCTGCTAATTGAGAAAGTGGATTCGGAGATTTTAAGTGCCAAGCTGGATTCTCTG CAAACTCTGAATGCTTGCTGTGCTGTGTATGGACAGAAGGAGCTGAAGGACTTCCTGCCCAGCCTTTGGG CTTCCATCCGTAGAGAGGTTTTCCAGACTGCTAGTGAGCGAGTAGAGGCAGAGGGCCTGGCAGCTCTCCA CTCCCTGACTGCGTGTCTGTCCTGCTCTGTGCTGAGAGCTGATGCTGAGGACCTCCTTGGTTCCTTCCTC AGCAACATCCTTCAAGACTGCAGGCACCATCTATGTGAACCAGACATGAAACTTGTATGGCCTAGCGCTA AGCTGTTGCAGGCAGCTGCAGGTGCGTCTGCCCGGGCCTGTGAGCACCTCACCAGCAACGTGCTTCCCTT GCTGCTGGAACAGTTCCACAAGCACAGCCAGAGCAACCAGCGTCGGACAATCCTTGAAATGATCCTGGGA TTCCTAAAGCTACAGCAGAAATGGAGCTATGAAGACAGAGATGAAAGGCCTCTGAGTAGCTTCAAAGACC AGCTATGCTCATTGGTGTTCATGGCTCTAACAGACCCCAGCACCCAGCTTCAGCTTGTTGGCATCCGGAC GCTCACAGTCTTGGGTGCCCAGCCAGGTCTCCTGTCTGCTGAGGACTTGGAGCTGGCAGTAGGTCACCTG TACCGACTGACCTTCCTGGAGGAAGATTCCCAGAGTTGTAGGGTGGCCGCACTGGAGGCATCAGGAACCC TGGCCACTCTGTATCCTGGAGCCTTCAGCAGACACTTGCTCCCCAAGCTTGCTGAGGAGCTGCATAAAGG GGAGTCAGATGTGGCTAGAGCAGATGGTCCCACCAAATGTTCCCGGCATTTCCGCTGTCTGCAAGCTTTG TCAGCTGTTTCAACACATCCCAGCATTGTCAAGGAGACACTGCCTCTGCTACTGCAGCATCTCTGTCAAG CAAACAAAGGGAATATGGTTACAGAGTCCAGTGAAGTTGTTGCTGTCTGTCAGAGTCTCCAGCAGGTGGC AGAAAAATGCCAGCAGGACCCTGAGAGCTACTGGTATTTCCACAAGACAGCTGTACCCTGCCTGTTTGCA TTGGCTGTGCAGGCTTCAATGCCAGAGAAGGAATCCTCAGTTCTGAGAAAAGTACTGTTGGAGGATGAGG TCTTGGCTGCGTTGGCATCTGTCATTGGCACTGCCACTACCCATCTGAGCCCTGAACTAGCTGCCCAGAG CGTCACATGCATCGTGCCCCTCTTCTTAGATGGCAACACTTCCTTTCTACCTGAAAACAGCTTCCCTGAC CAGTTCCAGCCGTTCCAGGATGGCTCCTCTGGGCAGAGGCGGCTGGTTGCACTACTTACGGCTTTTGTCT GCTCCCTGCCACGAAATGTGGAAATCCCTCAGCTGAACCGACTCATGCGGGAGCTTTTGAAGCAGAGCTG TGGCCACAGCTGTCCTTTCTCCTCCACTGCCGCCACTAAGTGCTTTGCAGGGCTCCTCAACAAGCAGCCT CCAGGGCAGCAGTTGGAGGAGTTCCTCCAGCTCGCTGTGGGCACAGTGGAGGCGGGCCTGGCCTCTGAAT CCTCCCGTGATCAGGCTTTCACACTGCTGCTCTGGGTAACCAAGGCTCTAGTCCTCAGATACCATCCTCT CAGTGCCTGCCTGACCACCCGGCTCATGGGCCTCCTCAGTGATCCAGAACTTGGCTGTGCAGCAGCTGAT GGCTTCTCTCTGCTCATGTCTGACTGCACTGATGTGTTGACTCGTGCCGGCCATGCTGATGTTCGGATCA TGTTCCGCCAGCGGTTCTTCACAGACAATGTGCCTGCTTTGGTCCAAGGTTTCCATGCCGCTCCCCAAGA TGTGAAGCCAAACTATCTGAAGGGTCTGTCTCACGTACTCAACAGGCTGCCCAAGCCTGTGCTTTTACCA GAGCTGCCCACACTTCTTTCCTTGCTGCTGGAAGCATTATCCTGCCCTGACTCTGTGGTCCAGCTTTCCA CTCTGAGCTGTCTCCAACCCCTACTACTGGAAGCACCTCAAATCATGAGTCTTCATGTTGACACTCTGGT TACAAAATTCCTGAACCTCAGCTCCAGTTACTCAATGGCTGTCCGGATTGCTGCTCTGCAGTGTATGCAC GCTCTCACTCGCCTCCCCACTTCTGTGCTACTGCCATACAAATCTCAGGTCATCCGAGCCTTAGCCAAGC CTCTGGACGACAAGAAGAGACTTGTGCGAAAGGAAGCAGTGTCAGCGAGGGGAGAATGGTTCCTACTTGG GAGCCCGGGGAGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG211485 representing NM_028152
Red=Cloning site Green=Tags(s) MAAATGLEEAVAPMGALCGLVQDFVMGQQEGPADQVAADVKSGGYTVLQVVEALGSSLENAEPRTRARGA QLLSQVLLQCHSLLSEKEVVHLILFYENRLKDHHLVVPSVLQGLRALSMSVALPPGLAVSVLKAIFQEVH VQSLLQVDRHTVFSIITNFMRSREEELKGLGADFTFGFIQVMDGEKDPRNLLLAFRIVHDLISKDYSLGP FVEELFEVTSCYFPIDFTPPPNDPYGIQREDLILSLRAVLASTPRFAEFLLPLLIEKVDSEILSAKLDSL QTLNACCAVYGQKELKDFLPSLWASIRREVFQTASERVEAEGLAALHSLTACLSCSVLRADAEDLLGSFL SNILQDCRHHLCEPDMKLVWPSAKLLQAAAGASARACEHLTSNVLPLLLEQFHKHSQSNQRRTILEMILG FLKLQQKWSYEDRDERPLSSFKDQLCSLVFMALTDPSTQLQLVGIRTLTVLGAQPGLLSAEDLELAVGHL YRLTFLEEDSQSCRVAALEASGTLATLYPGAFSRHLLPKLAEELHKGESDVARADGPTKCSRHFRCLQAL SAVSTHPSIVKETLPLLLQHLCQANKGNMVTESSEVVAVCQSLQQVAEKCQQDPESYWYFHKTAVPCLFA LAVQASMPEKESSVLRKVLLEDEVLAALASVIGTATTHLSPELAAQSVTCIVPLFLDGNTSFLPENSFPD QFQPFQDGSSGQRRLVALLTAFVCSLPRNVEIPQLNRLMRELLKQSCGHSCPFSSTAATKCFAGLLNKQP PGQQLEEFLQLAVGTVEAGLASESSRDQAFTLLLWVTKALVLRYHPLSACLTTRLMGLLSDPELGCAAAD GFSLLMSDCTDVLTRAGHADVRIMFRQRFFTDNVPALVQGFHAAPQDVKPNYLKGLSHVLNRLPKPVLLP ELPTLLSLLLEALSCPDSVVQLSTLSCLQPLLLEAPQIMSLHVDTLVTKFLNLSSSYSMAVRIAALQCMH ALTRLPTSVLLPYKSQVIRALAKPLDDKKRLVRKEAVSARGEWFLLGSPGS TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_028152 |
ORF Size | 3093 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_028152.3, NP_082428.1 |
RefSeq Size | 3965 bp |
RefSeq ORF | 3096 bp |
Locus ID | 72199 |
UniProt ID | Q9D071 |
Gene Summary | Key component of the cytosolic iron-sulfur protein assembly (CIA) complex, a multiprotein complex that mediates the incorporation of iron-sulfur cluster into apoproteins specifically involved in DNA metabolism and genomic integrity. In the CIA complex, MMS19 acts as an adapter between early-acting CIA components and a subset of cellular target Fe/S proteins such as ERCC2/XPD, FANCJ and RTEL1, thereby playing a key role in nucleotide excision repair (NER), homologous recombination-mediated double-strand break DNA repair, DNA replication and RNA polymerase II (POL II) transcription. As a CIA complex component and in collaboration with CIAO1 and CIAO2, binds to and facilitates the assembly of most cytosolic-nuclear Fe/S proteins. As part of the mitotic spindle-associated MMXD complex, plays a role in chromosome segregation, probably by facilitating iron-sulfur cluster assembly into ERCC2/XPD. Indirectly acts as a transcriptional coactivator of estrogen receptor (ER), via its role in iron-sulfur insertion into some component of the TFIIH-machinery.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC203350 | Mms19 (untagged) - Mouse MMS19 (MET18 S. cerevisiae) (Mms19), (10ug) |
CNY 7,184.00 |
|
MR211485 | Mms19 (Myc-DDK-tagged) - Mouse MMS19 (MET18 S. cerevisiae) (Mms19) |
CNY 7,176.00 |
|
MR211485L3 | Lenti ORF clone of Mms19 (Myc-DDK-tagged) - Mouse MMS19 (MET18 S. cerevisiae) (Mms19) |
CNY 10,260.00 |
|
MR211485L4 | Lenti ORF clone of Mms19 (mGFP-tagged) - Mouse MMS19 (MET18 S. cerevisiae) (Mms19) |
CNY 10,260.00 |