Herc4 (BC043082) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	1700056O17Rik; 4921531D01Rik; 9530080M15Rik; mKIAA1593
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG211530 representing BC043082 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTGTGCTGGGGCAATGCATCCTATGGACAACTAGGTTTGGGAGGAATTGATGAAGAAATTGTACTAG AGCCCAGGAGAAGTGACTTTTTCGTGAACAAAAAGGTCCGAGATGTAGGCTGTGGACTCAGGCACACTGT GTTTGTCCTGGATGATGGGACTGTGTACACATGTGGATGTAATGATCTAGGACAGCTAGGTCATGAAAAG TCCAGAAAGAAACCAGAGCAGGTTGTTGCCCTGGATGCCCAGAATATCGTAGCTGTTGCGTGTGGAGAAG CTCACACGTTAGCGCTGAATGACAAGGGCCAGGTGTATGCTTGGGGTCTCGACTCTGACGGACAGCTTGG CCTACAGGGATCAGAGGAATGTATCAGAGTACCCAGAAATATTAAAAGTCTTTCGGATATCCAGATAGTA CAGGTTGCGTGTGGTTACTATCATTCGCTTGCACTTTCTAAAGCAAGTGAAGTTTTCTGTTGGGGACAGA ATAAATATGGCCAGCTGGGTCTAGGCATTGATTGTCAAAAGCAAACTTCACCACAGCTGATTAAGTCTTT GCTTGGAATACCATTCATGCAAGTCGCAGCAGGAGGAGCCCATAGTTTTGTACTCACCCTTTCCGGAGCT ATCTTTGGATGGGGACGTAACAAATTTGGTCAGCTAGGTCTTAATGATGAAAATGATAGGTATGTTCCTA ATTTACTAAAGTCACTAAGATCTCAGAAAATAGTTTATATTTGTTGCGGAGAAGATCATACTGCTGCATT AACCAAGGAAGGTGGAGTGTTTACCTTTGGAGCTGGGGGCTATGGCCAGCTGGGTCATAATTCTACCAGT CATGAGATAAATCCCAGGAAAGTTTTTGAACTCATGGGAAGCATTGTTACTCAGGTCGCTTGTGGAAGGC AGCATACCTCTGCATTTGTTCCTTCTTCAGGACGAATTTACTCCTTTGGTCTTGGTGGTAATGGGCAGTT GGGAACTGGTTCAACCAGCAACAGAAAAAGCCCTTTCACTGTAAAAGGAAACTGGTTTTCTTATAATGGA CAGTGTCCACAAGATATTGGTTCTGAAGACTATTTCTGTGTCAAAAGAATTTTCTCAGGTGGAGATCAAA GCTTTTCACATTACTCTAGTCCTCAGAACTGTGGACCTCCAGATGACTTTAGATGCTCCGACCCTTCCAA GCAGATCTGGACAGTGAACGAAGCTCTGATTCAGAAATGGCTCAGCTACCCCTCTGGAAGGTTCCCTGTG GAGATAGCCAATGAGATAGATGGAACATTTTCTTCATCTGGCTGCCTAAATGGAAGTTTTCTGGCTATTA GCAATGACGATCACTATAGAACAGGTACCAGATTTTCAGGGGTTGATATGAACGCTGCTAGACTCTTATT CCACAAACTTATACAACCTGATCATCCTCAGATATCTCAGCAGGTGGCAGCTAGTTTGGAAAAGAATCTA ATCCCTAAACTGACTAGTTCCCTACCTGATGTTGAAGCTCTGAGGTTTTATCTTACCCTGCCAGAGTGTC CCCTGATGAGTGACTGCAACAATTTCACAACAATAGCAATCCCCTTTGGTACAGCTCTTGTGAACCTAGA AAAGGCACCACTGAAAGTACTTGAAAACTGGTGGTCAGTACTTGAACCTCCACTATTCCTCAAGATAGTA GAACTTTTTAAGGAAGTTGTGGTACATCTTTTGAAACTCTACAAGATCGGCATTCCCCCTTCTGAAAGAA GAATTTTCAACAGTTTTCTTCATACCGCATTAAAGGTTTTAGAAATATTGCATAGGGTAAATGAGAAAAC AGGACAACTTATTCAATATGACAAGTTTTACATCCATGAAGTCCAAGAGCTGATAGACATAAGGAATGAC TATATCAACTGGGTCCAGCAGCAAGCCTATGGAGTGTTGGCAGATATCCCGGTGACAATTTGTACATATC CATTTGTATTTGATGCCCAGGCAAAAACTACTCTGCTCCAGACAGATGCAGTCTTGCAGATGCAGATGGC CATTGACCAGGCCCACAGGCAGAATGTCTCCTCTCTTTTTCTCCCAGTGATCGAGTCTGTGAATCCTTGC TTAATTCTAGTTGTTCGCAGAGAGAATATTGTAGGAGATGCGATGGAAGTCCTCAGGAAAACCAAGAATA TAGATTATAAAAAGCCACTCAAGGTTATATTTGTTGGAGAAGATGCTGTTGATGCAGGAGGTGTTCGCAA AGAATTTTTCTTGCTTATCATGAGGGAATTGTTGGATCCTAAATACGGCATGTTTCGATATTATGAAGAT TCCAGGCTCATTTGGTTTTCAGATAAGACATTTGAAGACAGTGATTTGTTCCACTTGATCGGTGTTATCT GTGGATTAGCAATTTATAATTTTACCATTGTGGACCTCCATTTCCCTTTGGCTTTATATAAGAAACTGCT GAAAAGGAAGCCGTCCCTGGATGATCTGAAAGAGCTGATGCCAGCCGTCGGGAGAAGCATGCAGCAATTG CTGGACTATCCAGAAGATGACATAGAGGAAACATTTTGTCTAAACTTTACGATCACAGTTGAGAACTTCG GTGCAACAGAAGTAAAAGAGCTGGTTCTGAACGGGGCAGACACTGCTGTTAATAGACAGAATCGGCAGGA GTTTGTTGATGCCTATGTGGATTACATATTCAATAAATCAGTGGCATCTTTATTTGATGCTTTCCATGCG GGCTTTCATAAGGTCTGTGGAGGAAAAGTTCTTCTGCTCTTCCAGCCTAATGAATTACAAGCTATGGTTA TTGGGAATACAAATTATGATTGGAAAGAACTGGAGAAGAATACAGAATACAAAGGAGAGTATTGGGCAGA CCATCCCACGATAAAAATTTTTTGGGAAGTGTTTCATGAATTACCACTAGAGAAGAAGAAACAATTTCTA TTATTTTTGACAGGTAGTGATCGGATTCCCATTCTTGGTATGAAGAGTCTGAAACTAGTCATCCAGTCAA CAGGAGGTGGTGAGAGCTATCTCCCGGTTTCCCATACCTGTTTTAATCTTCTAGATCTCCCAAAATATAC AGAGAAAGAAACCCTCCGATGTAAACTGATCCAAGCTATTGATCACAATGAAGGCTTCAGTTTAATA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG211530 representing BC043082 Red=Cloning site Green=Tags(s) MLCWGNASYGQLGLGGIDEEIVLEPRRSDFFVNKKVRDVGCGLRHTVFVLDDGTVYTCGCNDLGQLGHEK SRKKPEQVVALDAQNIVAVACGEAHTLALNDKGQVYAWGLDSDGQLGLQGSEECIRVPRNIKSLSDIQIV QVACGYYHSLALSKASEVFCWGQNKYGQLGLGIDCQKQTSPQLIKSLLGIPFMQVAAGGAHSFVLTLSGA IFGWGRNKFGQLGLNDENDRYVPNLLKSLRSQKIVYICCGEDHTAALTKEGGVFTFGAGGYGQLGHNSTS HEINPRKVFELMGSIVTQVACGRQHTSAFVPSSGRIYSFGLGGNGQLGTGSTSNRKSPFTVKGNWFSYNG QCPQDIGSEDYFCVKRIFSGGDQSFSHYSSPQNCGPPDDFRCSDPSKQIWTVNEALIQKWLSYPSGRFPV EIANEIDGTFSSSGCLNGSFLAISNDDHYRTGTRFSGVDMNAARLLFHKLIQPDHPQISQQVAASLEKNL IPKLTSSLPDVEALRFYLTLPECPLMSDCNNFTTIAIPFGTALVNLEKAPLKVLENWWSVLEPPLFLKIV ELFKEVVVHLLKLYKIGIPPSERRIFNSFLHTALKVLEILHRVNEKTGQLIQYDKFYIHEVQELIDIRND YINWVQQQAYGVLADIPVTICTYPFVFDAQAKTTLLQTDAVLQMQMAIDQAHRQNVSSLFLPVIESVNPC LILVVRRENIVGDAMEVLRKTKNIDYKKPLKVIFVGEDAVDAGGVRKEFFLLIMRELLDPKYGMFRYYED SRLIWFSDKTFEDSDLFHLIGVICGLAIYNFTIVDLHFPLALYKKLLKRKPSLDDLKELMPAVGRSMQQL LDYPEDDIEETFCLNFTITVENFGATEVKELVLNGADTAVNRQNRQEFVDAYVDYIFNKSVASLFDAFHA GFHKVCGGKVLLLFQPNELQAMVIGNTNYDWKELEKNTEYKGEYWADHPTIKIFWEVFHELPLEKKKQFL LFLTGSDRIPILGMKSLKLVIQSTGGGESYLPVSHTCFNLLDLPKYTEKETLRCKLIQAIDHNEGFSLI TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC043082
ORF Size	3149 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC043082, AAH43082
RefSeq Size	3857 bp
RefSeq ORF	3149 bp
Locus ID	67345
Gene Summary	Probable E3 ubiquitin-protein ligase involved in either protein trafficking or in the distribution of cellular structures. Required for spermatozoon maturation and fertility, and for the removal of the cytoplasmic droplet of the spermatozoon. E3 ubiquitin-protein ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer it to targeted substrates.[UniProtKB/Swiss-Prot Function]

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