Smarca5 (BC053069) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	4933427E24Rik; D030040M08Rik; D330027N15Rik; MommeD4; Snf2h
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG211537 representing BC053069 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGTCCGCGGTGGAGCCTCCGCCGCCCCCGCCTCCCGAGAGCGCGCCTTCCAAGCCCTCGGCGGCGG GCGCCGGCGGGAGCAGCAGCGGCAACAAAGGCGGCCCCGAGGGCGGCGCGGCGCCCGCGGCTCCGTGTGC TGCGGGCTCGGGCCCCGCGGACACCGAGATGGAGGAAGTATTTGATCATGGATCACCTGGAAAGCAAAAA GAAATCCAAGAACCAGATCCTACATATGAAGAAAAAATGCAAACTGACCGAGCAAATAGATTTGAGTATT TATTAAAGCAGACAGAGCTGTTCGCACATTTCATTCAGCCTGCTGCTCAGAAGACTCCAACCTCACCCTT GAAGATGAAACCAGGGCGCCCACGGGTAAAAAAGGATGAAAAACAGAATTTGCTTTCAGTTGGAGATTAC CGACACCGTAGAACGGAGCAAGAGGAGGATGAAGAGCTATTAACAGAAAGTTCCAAAGCAACTAATGTTT GTACTCGATTTGAAGATTCTCCATCATATGTAAAATGGGGTAAACTGAGAGATTATCAGGTGCGAGGATT GAATTGGCTCATCTCTCTGTATGAGAATGGCATCAATGGGATCCTTGCAGATGAAATGGGTTTGGGAAAG ACACTTCAAACAATTTCTCTTCTTGGATACATGAAACACTATAGAAATATTCCTGGTCCTCATATGGTTT TGGTTCCCAAGTCTACATTGCACAACTGGATGAGTGAATTCAAGAAATGGGTACCAACACTTAGGTCTGT TTGCTTGATAGGAGATAAAGAGCAAAGAGCTGCATTTGTCAGGGATGTTTTATTACCAGGAGAGTGGGAT GTGTGTGTCACATCTTATGAAATGCTTATTAAAGAGAAGTCTGTGTTCAAAAAATTCAACTGGAGATACT TAGTTATAGATGAAGCTCACAGGATAAAAAATGAAAAATCTAAGTTGTCAGAAATAGTGAGAGAATTCAA GACTACAAATCGGCTGTTGCTAACAGGGACGCCTCTTCAGAACAACCTGCATGAGCTCTGGTCACTTCTA AACTTTTTGTTACCAGATGTGTTTAATTCAGCTGATGACTTTGATTCCTGGTTTGATACAAATAATTGCC TTGGGGATCAAAAGCTAGTTGAGAGGCTTCATATGGTTTTGCGTCCATTCCTCCTTCGTCGAATTAAAGC TGATGTTGAAAAGAGTTTGCCTCCAAAAAAAGAAGTAAAAATCTATGTGGGTCTTAGCAAAATGCAAAGA GAATGGTATACTCGGATATTAATGAAAGATATAGACATATTAAACTCAGCAGGGAAGATGGACAAAATGA GGCTATTGAACATTTTGATGCAGTTGAGGAAATGCTGCAATCATCCTTATCTCTTCGATGGAGCTGAACC TGGTCCACCGTACACAACAGATATGCATCTAGTTACCAACAGTGGCAAGATGGTGGTGTTAGACAAGCTA CTCCCTAAACTGAAAGAACAAGGTTCAAGAGTACTAATCTTTAGTCAGATGACAAGAGTATTAGACATTT TGGAAGATTATTGCATGTGGAGAAATTATGAGTACTGCAGGTTGGATGGACAGACACCCCATGATGAGAG ACAAGACTCCATCAATGCATACAATGAACCAAATAGCACAAAGTTTGTATTTATGCTAAGCACACGTGCT GGTGGTCTGGGCATCAATCTTGCAACTGCTGATGTAGTCATTTTATATGACTCAGATTGGAATCCGCAAG TTGATCTTCAGGCTATGGACCGTGCCCATAGAATTGGACAAACGAAGACTGTCCGAGTGTTTCGCTTTAT AACTGATAACACTGTGGAAGAAAGAATAGTGGAACGTGCTGAGATGAAACTTAGACTGGATTCGATAGTA ATTCAACAAGGGAGGCTTGTAGATCAGAACCTGAACAAAATTGGGAAAGATGAAATGCTTCAAATGATTC GACATGGTGCTACACATGTGTTTGCTTCAAAAGAAAGTGAAATCACTGATGAGGATATTGATGGTATTTT AGAAAGAGGTGCAAAGAAGACTGCAGAAATGAACGAAAAGCTCTCCAAGATGGGTGAAAGTTCACTTAGA AACTTTACGATGGACACAGAGTCCAGTGTTTATAACTTTGAAGGAGAAGACTATAGAGAAAAACAAAAGA TTGCATTCACAGAGTGGATTGAACCACCAAAAAGAGAAAGAAAAGCCAACTATGCTGTTGATGCATATTT CAGGGAAGCTCTCCGTGTTAGTGAGCCTAAAGCACCCAAGGCACCTCGACCTCCAAAACAACCAAATGTT CAGGATTTCCAGTTCTTTCCTCCGCGTTTATTTGAATTACTGGAAAAAGAAATTCTGTATTACAGGAAAA CTATTGGGTATAAGGTACCTCGGAGTCCTGATCTACCTAATGCAGCACAGGCACAGAAAGAGGAACAGCT AAAAATTGATGAAGCAGAGCCCCTCAATGACGAGGAGTTAGAGGAAAAAGAGAAGCTTCTGACACAGGGG TTTACAAATTGGAATAAGAGAGATTTTAACCAGTTTATCAAGGCTAATGAGAAGTGGGGTCGTGATGATA TTGAAAATATAGCCAGAGAAGTAGAAGGAAAAACTCCAGAAGAAGTCATTGAATACTCAGCTGTGTTCTG GGAAAGATGCAATGAGCTTCAGGACATAGAGAAGATTATGGCTCAGATTGAAAGGGGAGAGGCAAGAATT CAAAGAAGAATAAGTATCAAGAAAGCACTAGATACAAAGATTGGACGGTACAAAGCACCCTTTCATCAGC TAAGAATATCATATGGTACTAACAAAGGAAAAAACTATACTGAAGAAGAGGATCGTTTTCTCATTTGTAT GCTGCACAAGCTTGGCTTCGACAAGGAAAATGTGTATGATGAATTACGACAATGCATCCGAAACTCCCCT CAGTTTAGATTTGATTGGTTTCTCAAGTCCAGAACTGCGATGGAGCTCCAGAGGAGGTGTAATACCTTAA TTACTTTGATTGAAAGAGAAAATATGGAACTAGAAGAAAAGGAGAAGGCAGAGAAAAAGAAAAGAGGACC AAAACCTTCCACACAGAAACGTAAGATGGACGGTGCCCCTGATGGCCGTGGAAGAAAAAAGAAGCTGAAA CTA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG211537 representing BC053069 Red=Cloning site Green=Tags(s) MSSAVEPPPPPPPESAPSKPSAAGAGGSSSGNKGGPEGGAAPAAPCAAGSGPADTEMEEVFDHGSPGKQK EIQEPDPTYEEKMQTDRANRFEYLLKQTELFAHFIQPAAQKTPTSPLKMKPGRPRVKKDEKQNLLSVGDY RHRRTEQEEDEELLTESSKATNVCTRFEDSPSYVKWGKLRDYQVRGLNWLISLYENGINGILADEMGLGK TLQTISLLGYMKHYRNIPGPHMVLVPKSTLHNWMSEFKKWVPTLRSVCLIGDKEQRAAFVRDVLLPGEWD VCVTSYEMLIKEKSVFKKFNWRYLVIDEAHRIKNEKSKLSEIVREFKTTNRLLLTGTPLQNNLHELWSLL NFLLPDVFNSADDFDSWFDTNNCLGDQKLVERLHMVLRPFLLRRIKADVEKSLPPKKEVKIYVGLSKMQR EWYTRILMKDIDILNSAGKMDKMRLLNILMQLRKCCNHPYLFDGAEPGPPYTTDMHLVTNSGKMVVLDKL LPKLKEQGSRVLIFSQMTRVLDILEDYCMWRNYEYCRLDGQTPHDERQDSINAYNEPNSTKFVFMLSTRA GGLGINLATADVVILYDSDWNPQVDLQAMDRAHRIGQTKTVRVFRFITDNTVEERIVERAEMKLRLDSIV IQQGRLVDQNLNKIGKDEMLQMIRHGATHVFASKESEITDEDIDGILERGAKKTAEMNEKLSKMGESSLR NFTMDTESSVYNFEGEDYREKQKIAFTEWIEPPKRERKANYAVDAYFREALRVSEPKAPKAPRPPKQPNV QDFQFFPPRLFELLEKEILYYRKTIGYKVPRSPDLPNAAQAQKEEQLKIDEAEPLNDEELEEKEKLLTQG FTNWNKRDFNQFIKANEKWGRDDIENIAREVEGKTPEEVIEYSAVFWERCNELQDIEKIMAQIERGEARI QRRISIKKALDTKIGRYKAPFHQLRISYGTNKGKNYTEEEDRFLICMLHKLGFDKENVYDELRQCIRNSP QFRFDWFLKSRTAMELQRRCNTLITLIERENMELEEKEKAEKKKRGPKPSTQKRKMDGAPDGRGRKKKLK L TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC053069
ORF Size	3155 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC053069, AAH53069
RefSeq Size	4658 bp
RefSeq ORF	3155 bp
Locus ID	93762
Gene Summary	Helicase that possesses intrinsic ATP-dependent nucleosome-remodeling activity. Complexes containing SMARCA5 are capable of forming ordered nucleosome arrays on chromatin; this may require intact histone H4 tails. Also required for replication of pericentric heterochromatin in S-phase specifically in conjunction with BAZ1A. Probably plays a role in repression of polI dependent transcription of the rDNA locus, through the recruitment of the SIN3/HDAC1 corepressor complex to the rDNA promoter. Essential component of the WICH complex, a chromatin remodeling complex that mobilizes nucleosomes and reconfigures irregular chromatin to a regular nucleosomal array structure. The WICH complex regulates the transcription of various genes, has a role in RNA polymerase I and RNA polymerase III transcription, mediates the histone H2AX phosphorylation at 'Tyr-142', and is involved in the maintenance of chromatin structures during DNA replication processes. Essential component of the NoRC (nucleolar remodeling complex) complex, a complex that mediates silencing of a fraction of rDNA by recruiting histone-modifying enzymes and DNA methyltransferases, leading to heterochromatin formation and transcriptional silencing.[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.