Tek (BC050824) Mouse Tagged ORF Clone

CAT#: MG211587

  • TrueORF®

Tek (tGFP-tagged) - Mouse endothelial-specific receptor tyrosine kinase (cDNA clone MGC:59299 IMAGE:6333576)

ORF Plasmid: DDK tGFP



  "BC050824" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 11,780.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms AA517024; Cd202b; Hyk; STK1; Tie-2; Tie2
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG211587 representing BC050824
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGACTCTTTAGCCGGCTTAGTTCTCTGTGGAGTCAGCTTGCTCCTTTATGGAGTAGTAGAAGGCGCCA
TGGACCTGATCTTGATCAATTCCCTACCTCTTGTGTCTGATGCCGAAACATCCCTCACCTGCATTGCCTC
TGGGTGGCACCCCCATGAGCCCATCACCATAGGAAGGGACTTTGAAGCCTTAATGAACCAGCACCAAGAT
CCACTGGAGGTTACTCAAGATGTGACCAGAGAATGGGCGAAAAAAGTTGTTTGGAAGAGAGAAAAGGCCA
GTAAGATTAATGGTGCTTATTTCTGTGAAGGTCGAGTTCGAGGACAGGCTATAAGGATACGGACCATGAA
GATGCGTCAACAAGCATCCTTCCTACCTGCTACTTTAACTATGACCGTGGACAGGGGAGATAATGTGAAC
ATATCTTTCAAAAAGGTGTTAATTAAAGAAGAAGATGCAGTGATTTACAAAAATGGATGTGAAGCTCAGA
AGTGGGGGCCCGACTGTAGCCGTCCTTGTACTACTTGCAAGAACAATGGAGTCTGCCATGAAGATACCGG
GGAATGCATTTGCCCTCCTGGGTTTATGGGGAGAACATGTGAGAAAGCTTGTGAGCCGCACACATTTGGC
AGGACCTGTAAAGAAAGGTGTAGTGGACCAGAAGGATGCAAGTCTTATGTGTTCTGTCTCCCAGACCCTT
ACGGGTGTTCCTGTGCCACAGGCTGGAGGGGGTTGCAGTGCAATGAAGCATGCCCATCTGGTTACTACGG
ACCAGACTGTAAGCTCAGGTGCCACTGTACCAATGAAGAGATATGTGATCGGTTCCAAGGATGCCTCTGC
TCTCAAGGATGGCAAGGGCTGCAGTGTGAGAAAGAAGGCAGGCCAAGGATGACTCCACAGATAGAGGATT
TGCCAGATCACATTGAAGTAAACAGTGGAAAATTTAACCCCATCTGCAAAGCCTCTGGGTGGCCACTACC
TACTAGTGAAGAAATGACCCTAGTGAAGCCAGATGGGACAGTGCTCCAACCAAATGACTTCAACTATACA
GATCGTTTCTCAGTGGCCATATTCACTGTCAACCGAGTCTTACCTCCTGACTCAGGAGTCTGGGTCTGCA
GTGTGAACACAGTGGCTGGGATGGTGGAAAAGCCTTTCAACATTTCCGTCAAAGTTCTTCCAGAGCCCCT
GCACGCCCCAAATGTGATTGACACTGGACATAACTTTGCTATCATCAATATCAGCTCTGAGCCTTACTTT
GGGGATGGACCCATCAAATCCAAGAAGCTTTTCTATAAACCTGTCAATCAGGCCTGGAAATACATTGAAG
TGACGAATGAGATTTTCACTCTCAACTACTTGGAGCCGCGGACTGACTACGAGCTGTGTGTGCAGCTGGC
CCGTCCTGGAGAGGGTGGAGAAGGGCATCCTGGGCCTGTGAGACGATTTACAACAGCGTCTATCGGACTC
CCTCCTCCAAGAGGTCTCAGTCTCCTGCCAAAAAGCCAGACAGCTCTAAATTTGACTTGGCAACCGATAT
TTACAAACTCAGAAGATGAATTTTATGTGGAAGTCGAGAGGCGATCCCTGCAAACAACAAGTGATCAGCA
GAACATCAAAGTGCCTGGGAACCTGACCTCGGTGCTACTGAGCAACTTAGTCCCCAGGGAGCAGTACACA
GTCCGAGCTAGAGTCAACACCAAGGCGCAGGGGGAGTGGAGTGAAGAACTCAGGGCCTGGACCCTTAGTG
ACATTCTCCCTCCTCAACCAGAAAACATCAAGATCTCCAACATCACTGACTCCACAGCTATGGTTTCTTG
GACAATAGTGGATGGCTATTCGATTTCTTCCATCATCATCCGGTATAAGGTTCAGGGCAAAAATGAAGAC
CAGCACATTGATGTGAAGATCAAGAATGCTACCGTTACTCAGTACCAGCTCAAGGGCCTAGAGCCAGAGA
CTACATACCATGTGGATATTTTTGCTGAGAACAACATAGGATCAAGCAACCCAGCCTTTTCTCATGAACT
GAGGACGCTTCCACATTCCCCAGCCTCTGCAGACCTCGGAGGGGGAAAGATGCTACTCATAGCCATCCTT
GGGTCGGCTGGAATGACTTGCATCACCGTGCTGTTGGCGTTTCTGATTATGTTGCAACTGAAGAGAGCAA
ATGTCCAAAGGAGAATGGCTCAGGCATTCCAGAACGTGAGAGAAGAACCAGCTGTGCAGTTTAACTCAGG
AACTCTGGCCCTTAACAGGAAGGCCAAAAACAATCCGGATCCCACAATTTATCCTGTGCTTGACTGGAAT
GACATCAAGTTTCAAGACGTGATCGGAGAGGGCAACTTTGGCCAGGTTCTGAAGGCACGCATCAAGAAGG
ATGGGTTACGGATGGATGCCGCCATCAAGAGGATGAAAGAGTATGCCTCCAAAGATGATCACAGGGACTT
CGCAGGAGAACTGGAGGTTCTTTGTAAACTTGGACACCATCCAAACATCATTAATCTCTTGGGAGCATGT
GAACACCGAGGCTATTTGTACCTAGCTATTGAGTATGCCCCGCATGGAAACCTCCTGGACTTCCTGCGTA
AGAGCAGAGTGCTAGAGACAGACCCTGCTTTTGCCATCGCCAACAGTACAGCTTCCACACTGTCCTCCCA
ACAGCTTCTTCATTTTGCTGCAGATGTGGCCCGGGGGATGGACTACTTGAGCCAGAAACAGTTTATCCAC
AGGGACCTGGCTGCCAGAAACATTTTAGTTGGTGAAAACTACATAGCCAAAATAGCAGATTTTGGATTGT
CACGAGGTCAAGAAGTGTATGTGAAAAAGACAATGGGAAGGCTCCCAGTGCGTTGGATGGCAATCGAATC
ACTGAACTATAGTGTCTATACAACCAACAGTGATGTCTGGTCCTATGGTGTATTGCTCTGGGAGATTGTT
AGCTTAGGAGGCACCCCCTACTGCGGCATGACGTGCGCGGAGCTCTATGAGAAGCTACCCCAGGGCTACA
GGCTGGAGAAGCCCCTGAACTGTGATGATGAGGTGTATGATCTAATGAGACAGTGCTGGAGGGAGAAGCC
TTATGAGAGACCATCATTTGCCCAGATATTGGTGTCCTTAAACAGGATGCTGGAAGAACGGAAGACATAC
GTGAACACCACACTGTATGAGAAGTTTACCTATGCAGGAATTGACTGCTCTGCGGAAGAAGCAGCC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG211587 representing BC050824
Red=Cloning site Green=Tags(s)

MDSLAGLVLCGVSLLLYGVVEGAMDLILINSLPLVSDAETSLTCIASGWHPHEPITIGRDFEALMNQHQD
PLEVTQDVTREWAKKVVWKREKASKINGAYFCEGRVRGQAIRIRTMKMRQQASFLPATLTMTVDRGDNVN
ISFKKVLIKEEDAVIYKNGCEAQKWGPDCSRPCTTCKNNGVCHEDTGECICPPGFMGRTCEKACEPHTFG
RTCKERCSGPEGCKSYVFCLPDPYGCSCATGWRGLQCNEACPSGYYGPDCKLRCHCTNEEICDRFQGCLC
SQGWQGLQCEKEGRPRMTPQIEDLPDHIEVNSGKFNPICKASGWPLPTSEEMTLVKPDGTVLQPNDFNYT
DRFSVAIFTVNRVLPPDSGVWVCSVNTVAGMVEKPFNISVKVLPEPLHAPNVIDTGHNFAIINISSEPYF
GDGPIKSKKLFYKPVNQAWKYIEVTNEIFTLNYLEPRTDYELCVQLARPGEGGEGHPGPVRRFTTASIGL
PPPRGLSLLPKSQTALNLTWQPIFTNSEDEFYVEVERRSLQTTSDQQNIKVPGNLTSVLLSNLVPREQYT
VRARVNTKAQGEWSEELRAWTLSDILPPQPENIKISNITDSTAMVSWTIVDGYSISSIIIRYKVQGKNED
QHIDVKIKNATVTQYQLKGLEPETTYHVDIFAENNIGSSNPAFSHELRTLPHSPASADLGGGKMLLIAIL
GSAGMTCITVLLAFLIMLQLKRANVQRRMAQAFQNVREEPAVQFNSGTLALNRKAKNNPDPTIYPVLDWN
DIKFQDVIGEGNFGQVLKARIKKDGLRMDAAIKRMKEYASKDDHRDFAGELEVLCKLGHHPNIINLLGAC
EHRGYLYLAIEYAPHGNLLDFLRKSRVLETDPAFAIANSTASTLSSQQLLHFAADVARGMDYLSQKQFIH
RDLAARNILVGENYIAKIADFGLSRGQEVYVKKTMGRLPVRWMAIESLNYSVYTTNSDVWSYGVLLWEIV
SLGGTPYCGMTCAELYEKLPQGYRLEKPLNCDDEVYDLMRQCWREKPYERPSFAQILVSLNRMLEERKTY
VNTTLYEKFTYAGIDCSAEEAA

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC050824
ORF Size 3216 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC050824.1
RefSeq Size 4549 bp
RefSeq ORF 3218 bp
Locus ID 21687
Gene Summary Tyrosine-protein kinase that acts as cell-surface receptor for ANGPT1, ANGPT2 and ANGPT4 and regulates angiogenesis, endothelial cell survival, proliferation, migration, adhesion and cell spreading, reorganization of the actin cytoskeleton, but also maintenance of vascular quiescence. Has anti-inflammatory effects by preventing the leakage of proinflammatory plasma proteins and leukocytes from blood vessels. Required for normal angiogenesis and heart development during embryogenesis. Required for post-natal hematopoiesis. After birth, activates or inhibits angiogenesis, depending on the context. Inhibits angiogenesis and promotes vascular stability in quiescent vessels, where endothelial cells have tight contacts. In quiescent vessels, ANGPT1 oligomers recruit TEK to cell-cell contacts, forming complexes with TEK molecules from adjoining cells, and this leads to preferential activation of phosphatidylinositol 3-kinase and the AKT1 signaling cascades. In migrating endothelial cells that lack cell-cell adhesions, ANGT1 recruits TEK to contacts with the extracellular matrix, leading to the formation of focal adhesion complexes, activation of PTK2/FAK and of the downstream kinases MAPK1/ERK2 and MAPK3/ERK1, and ultimately to the stimulation of sprouting angiogenesis. ANGPT1 signaling triggers receptor dimerization and autophosphorylation at specific tyrosine residues that then serve as binding sites for scaffold proteins and effectors. Signaling is modulated by ANGPT2 that has lower affinity for TEK, can promote TEK autophosphorylation in the absence of ANGPT1, but inhibits ANGPT1-mediated signaling by competing for the same binding site. Signaling is also modulated by formation of heterodimers with TIE1, and by proteolytic processing that gives rise to a soluble TEK extracellular domain. The soluble extracellular domain modulates signaling by functioning as decoy receptor for angiopoietins. TEK phosphorylates DOK2, GRB7, GRB14, PIK3R1, SHC1 and TIE1.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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