Wdr62 (BC054747) Mouse Tagged ORF Clone

CAT#: MG211595

  • TrueORF®

Wdr62 (tGFP-tagged) - Mouse WD repeat domain 62 (cDNA clone MGC:65624 IMAGE:6405269)

ORF Plasmid: DDK tGFP



  "BC054747" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 6,840.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms MGC31423
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG211595 representing BC054747
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTGCCTTAGCGGCCGGAGGTTATACGCGGAGTGACACGATAGAAAAGCTGTCCTCTGTCATGGCGG
GAGTTCCGGCGCGGAGAAACCAGTCCTCCCCGCCTCCTGCCCCACCGCTCTGCCTCCGGCGGCGGACGCG
ACTCGCGGCGGCTCCCGAGGACACTGTGCAGAACCGGGTGACACTTGAGAAGGTGCTTGGCATCACAGCC
CAGAACAGCAGCGGGCTAACCTGTGACCCTGGCACAGGCCATGTGGCCTACTTAGCAGGGTGCGTGGTGG
TGGTCTTGAACCCCAAGGAGAACAAGCAGCAGCATATATTTAACACAACCAGGAAGTCCCTGAGTGCTCT
GGCCTTCTCCCCAGATGGGAAGTACATAGTGACAGGAGAGAATGGACACCGGCCAGCTGTGCGCATCTGG
GATGTGGAGGAGAAGACTCAAGTGGCAGAGATGCTGGGCCACAAGTATGGTGTGGCCTGTGTGGCTTTCT
CACCCAATATGAAGCACATCGTGTCCATGGGCTACCAACATGACATGGTCCTCAATGTTTGGGATTGGAA
GAAAGACATTGTGGTGGCTTCCAACAAGGTGTCATGTAGAGTCATCGCTCTCTCCTTCTCTGAGGACAGC
AGCTATTTTGTCACCGTTGGGAATCGGCATGTGAGGTTTTGGTTCTTAGAAGCCTCTACTGAGGCCAAGG
TAACCAGCACAGTGCCCCTGGTAGGACGCTCAGGCATCCTGGGTGAGCTGCACAACAATATCTTTTGTGG
CGTGGCCTGCGGCCGGGGCCGGATGGCAGGCAATACCTTCTGTGTGTCCTACTCTGGCCTCCTCTGCCAG
TTCAATGAGAAAAGGGTGCTGGACAAGTGGATCAACCTGAAGGTCTCCTTGTCTTCCTGCCTGTGTGTCA
GTGACGAGTTGATCTTCTGTGGATGCACAGACGGGATAGTCCGCATCTTCCAGGCCCACAGCCTACTCTA
CCTCACCAACCTGCCCAAACCACACTACCTGGGAGTGGACGTGGCCCACGGACTGGACTCCAGCTTCCTC
TTCCACAGAAAAGCAGAAGCAGTCTACCCAGATACAGTGGCCCTGACCTTTGACCCCGTCCACCAGTGGC
TGTCCTGTGTTTACAAAGACCACAGCATCTACATCTGGGATGTCAAGGACATTGATGAAGTCAGCAAGAT
ATGGTCAGAGCTCTTCCACAGCTCCTTTGTCTGGAATGTAGAGGTGTACCCTGAATTTGAAGACCAGAGA
GCTTGCCTGCCGTCCGGGACTTTTCTGACTTGTTCCTCAGACAATACCATCCGCTTCTGGAATTTGGATA
GCGCCTCTGACACTCGATGGCAAAAGAACATCTTCAGCGATTCTCTGCTGAAGGTGGTCTATGTAGAGAA
TGACATCCAGCACCTGCAGGACCTGTCTCACTTCCCAGACCGGGGCAGTGAGAACGGCACTCCCATGGAT
ATGAAAGCTGGGGTTCGAGTCATGCAGGTCAGTCCTGACGGCCAGCACTTGGCTTCAGGCGACCGCAGTG
GAAATCTGAGGATCCACGAGCTGCACTTCATGGATGAGCTGATCAAGGTGGAGGCCCACGATGCTGAGGT
GCTGTGCCTGGAGTACTCCAAGCCTGAGACAGGAGTGACTTTGCTGGCTTCAGCCAGTCGGGACAGACTC
ATCCATGTGTTAAACGTGGAGAAGAACTACAACCTGGAGCAGACCCTGGACGACCACTCCTCCTCCATCA
CAGCCATTAAGTTTGCTGGCACCAGAGATGTCCAGATGATCAGTTGTGGAGCTGACAAGAGCATCTACTT
TCGCAGTGCCCAGCAGGCCTCGGATGGACTGCACTTTGTCCGTACCCACCACGTAGCAGAGAAGACCACC
TTGTATGACATGGATATCGACATCACACAGAAGTATGTGGCAGTGGCCTGCCAGGACCGCAACGTAAGGG
TCTACAACACCGTGAGTGGGAAACAGAAGAAGTGCTACAAGGGTTCCCAGGGCGACGAAGGGTCCCTGCT
GAAGGTCCACGTGGACCCCTCAGGCACCTTCCTGGCCACAAGCTGCTCTGACAAAAGCATCTCCTTGATT
GACTTTTACTCGGGCGAGTGTGTTGCCAAGATGTTTGGCCATTCGGAAATTGTCACTGGCATGAAGTTCA
CCTACGACTGCCGCCACTTGATCACAGTATCTGGAGACAGCTGTGTGTTCATCTGGCACCTGGGCCCGGA
GATCACCACCTGCATGAAGCAGCACTTGCTGGAGATCAACCACCAGGAGCAGCAGCAGCAGCCCAAGGAC
CAGAAGTGGAGTGGCCCTCCCAGCCAGGAGACATATGCATCCACACCAAGTGAGATTCGTTCCCTGAGCC
CTGGAGAGCAGACAGAGGATGAGATGGAGGAGGAATGTGAGCCAGAAGAGTTGCTGAAAACACCATCCAA
AGACAGCTTGGACCCAGATCCTCGATGCCTGCTGACAAATGGCAAGCTGCCACTCTGGGCAAAGCGGCTG
CTAGGGGATGATGATGTGGCAGACAGCTCAGCTTTCCACGCCAAGCGCAGCTACCAGCCACATGGCCGTT
GGGCAGAGCGGGCTGAGCAGGAACCCCTCAAGACCATCCTGGATGCCTGGAGCCTGGATTCCTACTTTAC
ACCCATGAAGCCTGAAAATCTCCAAGACTCTGTTCTGGACTCAGTAGAGCCACAGAACCTGGCAGGCCTG
CTAAGTGAGTGTTCACTCGGTAATGGACACACGTCTCCGGGAGAAGGCTTGGTGAGCTACCTACTTCACC
CAGAGTTAGGGAGTCCCAAAGAGGACAACCGAGGCCACCCCTCCTACCTGCCTCTACAGAGGGAAGCCAC
CGAGGCAAGCGAACTCATCCTCTGCTCCCCAGAGGCGGAAGTGTCACTTACAGGGATGCACAGGGAGTAT
TATGAGGAGGAGACAGAGGCAGGACCTGAAGACCAGCAAGGCGACACCTATCTCAGGGTCTCTTCCGTCA
GTTCAAAGGATCAGAGCCCCCCTGAGGACTCAGGGGAGTCAGAGGCTGAACTGGAGTGCAGCTTTGCCGC
CGCCCACAGCTCAGCTCCTCAAACGGACCCTGGCCCTCACCTCACCATGACAGCAGCGTCGTCCGCCGTG
ACTCAGAGTGCTGACAAGTCCTCTCCACCCTGCCTCCCATCCAGAGTACCCAAGTACAGAAGAGCTTTCC
CAGCC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG211595 representing BC054747
Red=Cloning site Green=Tags(s)

MAALAAGGYTRSDTIEKLSSVMAGVPARRNQSSPPPAPPLCLRRRTRLAAAPEDTVQNRVTLEKVLGITA
QNSSGLTCDPGTGHVAYLAGCVVVVLNPKENKQQHIFNTTRKSLSALAFSPDGKYIVTGENGHRPAVRIW
DVEEKTQVAEMLGHKYGVACVAFSPNMKHIVSMGYQHDMVLNVWDWKKDIVVASNKVSCRVIALSFSEDS
SYFVTVGNRHVRFWFLEASTEAKVTSTVPLVGRSGILGELHNNIFCGVACGRGRMAGNTFCVSYSGLLCQ
FNEKRVLDKWINLKVSLSSCLCVSDELIFCGCTDGIVRIFQAHSLLYLTNLPKPHYLGVDVAHGLDSSFL
FHRKAEAVYPDTVALTFDPVHQWLSCVYKDHSIYIWDVKDIDEVSKIWSELFHSSFVWNVEVYPEFEDQR
ACLPSGTFLTCSSDNTIRFWNLDSASDTRWQKNIFSDSLLKVVYVENDIQHLQDLSHFPDRGSENGTPMD
MKAGVRVMQVSPDGQHLASGDRSGNLRIHELHFMDELIKVEAHDAEVLCLEYSKPETGVTLLASASRDRL
IHVLNVEKNYNLEQTLDDHSSSITAIKFAGTRDVQMISCGADKSIYFRSAQQASDGLHFVRTHHVAEKTT
LYDMDIDITQKYVAVACQDRNVRVYNTVSGKQKKCYKGSQGDEGSLLKVHVDPSGTFLATSCSDKSISLI
DFYSGECVAKMFGHSEIVTGMKFTYDCRHLITVSGDSCVFIWHLGPEITTCMKQHLLEINHQEQQQQPKD
QKWSGPPSQETYASTPSEIRSLSPGEQTEDEMEEECEPEELLKTPSKDSLDPDPRCLLTNGKLPLWAKRL
LGDDDVADSSAFHAKRSYQPHGRWAERAEQEPLKTILDAWSLDSYFTPMKPENLQDSVLDSVEPQNLAGL
LSECSLGNGHTSPGEGLVSYLLHPELGSPKEDNRGHPSYLPLQREATEASELILCSPEAEVSLTGMHREY
YEEETEAGPEDQQGDTYLRVSSVSSKDQSPPEDSGESEAELECSFAAAHSSAPQTDPGPHLTMTAASSAV
TQSADKSSPPCLPSRVPKYRRAFPA

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC054747
ORF Size 3227 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC054747, AAH54747
RefSeq Size 4801 bp
RefSeq ORF 3227 bp
Locus ID 233064
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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