Rfc1 (NM_011258) Mouse Tagged ORF Clone
CAT#: MG211679
- TrueORF®
Rfc1 (tGFP-tagged) - Mouse replication factor C (activator 1) 1 (Rfc1)
ORF Plasmid: DDK
"NM_011258" in other vectors (4)
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CNY 10,830.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | 140kDa; Alp145; Recc1; RFC140 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG211679 representing NM_011258
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACATTCGGAAATTCTTTGGGGTTATATCAAGTGGAAAAAAGCCTGTAAACGAGACAGTAAAGAATG AGAAGACAAAAGCTTCGGAGGGAACTGTCAAAGGAAAAAAGGGAGTCAAGGAGGCCAAGGTCAATAACTC CGGTAAAGAGGACGCCTCCAAACCAAAGCAGCACAGCAAGAAGAAGAGGATCATCAATGACTCAGACTCA GAGTCAGAGGAGACAGTACAGGTAAAAAATGCTAAAAAGAAATCAGAAAAATTGTCACTGTCTTATAAAC CTGGTAAAGTCTCTCAGAAGGATCCTGTCACCTACGTCTCTGAGACAGATGAAGATGATGACTTTGTATG TAAAAAGGCAGCCTCCAAATCAAAAGAGAATGGAGTATCTACAAATAGTTACCTTGGAACATCAAACGTG AAAAAAAATGAAGAAAACGTTAAGACTAAGAACAAGCCGTTATCTCCAATAAAACTCACACCAACGTCAG TGCTCGACTATTTTGGAACTGAAAGTGTCCAGAGATCTGGGAAGAAGATGGTGACAAGCAAAAGGAAAGA ATCTTCTCAAAACACAGAGGATTCCAGATTAAATGATGAGGCCATCGCCAAGCAGCTGCAGCTGGATGAA GATGCAGAGCTGGAGAGGCAGTTGCATGAAGATGAAGAATTTGCAAGAACACTGGCCTTATTGGATGAAG AACCCAAGATCAAAAAGGCCCGAAAGGATTCTGAAGAGGGAGAAGAATCATTTTCATCTGTCCAAGATGA TTTAAGCAAAGCAGAAAAGCAGAAAAGCCCTAATAAAGCCGAGCTTTTCTCAACTGCAAGAAAGACCTAC AGTCCTGCTAAGCATGGCAAGGGGAGGGCTTCAGAAGATGCTAAGCAGCCTTGCAAATCAGCTCACCGGA AGGAAGCCTGTTCCTCACCCAAGGCCAGTGCCAAACTGGCGCTTATGAAAGCAAAAGAAGAAAGTTCTTA CAACGAAACAGAGCTGCTGGCTGCAAGAAGAAAAGAAAGCGCCACTGAACCCAAAGGAGAGAAAACAACT CCTAAGAAAACGAAAGTTTCTCCAACTAAGAGAGAGTCTGTAAGCCCAGAAGACTCTGAAAAGAAGCGCA CTAATTATCAAGCTTATCGAAGCTACTTGAACCGAGAAGGTCCCAAAGCTCTGGGCTCCAAAGAAATACC CAAGGGAGCTGAAAATTGCTTGGAAGGCCTGACGTTTGTGATCACAGGAGTGCTGGAGTCCATTGAACGA GACGAAGCCAAGTCTCTAATTGAACGTTATGGGGGGAAAGTAACAGGAAACGTGAGCAAGAAAACCAACT ACCTCGTCATGGGCCGCGACAGTGGGCAGTCCAAGAGTGACAAGGCAGCAGCTCTGGGAACAAAAATCCT CGATGAAGACGGCCTGTTGGATCTGATTCGAACTATGCCAGGCAAGAGATCCAAGTATGAGATGGCTGCT GAGGCTGAGATGAAGAAAGAAAAGTCCAAATTGGAGCGAACACCCCAAAAAAATGATCAAGGAAAAAGAA AAATTAGTCCAGCCAAGAAGGAGTCAGAGTCTAAAAAGTGCAAGCTGACTCTCCTAAAGAACAGTCCTAT GAAGGCAGTGAAGAAGGAGGCAAGCACGTGCCCAAGGGGCCTGGATGTCAAGGAGACACATGGCAACCGC AGTAGCAACAAAGAGGAATGTCTGCTCTGGGTGGATAAGTACAAGCCCGCCTCACTCAAGAACATAATCG GACAGCAGGGTGACCAGAGCTGTGCCAACAAATTACTACGTTGGCTCAGAAACTGGCACAAGAGTTCTCC TGAAGAGAAAAAACATGCAGCAAAGTTTGGCAAACTTGCCAGCAAAGATGATGGCTCCAGTTTCAAGGCA GCTCTGCTGTCTGGCCCTCCAGGTGTTGGCAAAACCACTACAGCTTCTCTTGTGTGTCAGGAATTGGGCT ACAGTTATGTGGAACTAAATGCAAGTGATACTCGGAGTAAGAACAGTTTGAAAGCAGTTGTTGCTGAATC CTTGAACAACACCAGCATCAAAGGCTTTTATACAAGTGGAGCGGCTCCTTCCGTGAGTGCGAGGCACGCC CTCATCATGGATGAGGTCGATGGCATGGCAGGCAATGAAGACAGGGGTGGAATTCAGGAGCTAATAGGCC TGATAAAGCATACTAAAATTCCCATCATTTGTATGTGCAATGATAGAAACCACCCCAAAATTCGCTCATT GGTTCATTATTGTTTTGATCTTCGTTTCCAAAGACCTCGAGTTGAACAGATTAAGAGTGCAATGCTGTCT ATTGCATTTAAAGAGGGTTTAAAAATCCCCCCTCCAGCTATGAATGAAATAATTTTGGGAGCTAATCAAG ATGTCAGACAGGTTTTACACAATCTCAGTATGTGGTGTGCACAAAGTAAGGCCCTGACCTATGACCAAGC CAAGGCTGATTCTCAGAGGGCTAAGAAGGATATCAGACTGGGCCCATTTGATGTCACTCGGAAAGTGTTT GCAGCTGGAGAGGAGACTGCACACATGTCACTGATGGACAAGTCAGATCTCTTCTTCCATGACTATTCCA TAGCACCCCTCTTTGTCCAGGAGAACTACCTGCATGTAAAGCCTGTGGCTGCAGGAGGTGACATGAAAAA GCACTTGATGCTTTTAAGCCGGGCAGCAGATAGCATATGTGACGGTGACTTAGTGGACAATCAGATCCGG AGTAAGCAAAACTGGAGTCTTCTGCCCACCCAGGCCATCTATGCCAGTGTTCTTCCTGGAGAGTTGATGC GGGGCTACATGACTCAGTTTCCGAGCTTCCCGAGCTGGCTGGGGAAGCACTCGTCTACGGGCAAACATGA TCGGATTGTTCAGGACCTATCCTTGCACATGAGTCTTAGAACTTACTCCAGCAAAAGGACAGTGAACATG GATTACCTGTCACACATCAGAGACGCACTTGTACGACCCTTGACCTCACAAGGGGTAGAAGGGGCTCAGC ATGTCATCAAACTTATGGACACATACTATCTGATGAAAGAAGATTTTGAGAACATCATGGAAGTTAGCAG CTGGGGGGGCAAGCCCAGTGCCTTTTCCAAGCTGGATCCCAAGGTGAAAGCGGCCTTCACAAGAGCCTAC AATAAGGAGGCCCACCTGACCCCATACTCACTTCAGGTAGTAAAGACATCGAGGCTCAGCACGGGTCCAG CACTGGATTCTGAGTACAGTGAGGAGTTCCAGGAAGATGACACTCAGTCTGAGAAAGAGCAGGATGCAGT GGAGACTGACGCCATGATCAAGAAAAAGACAAGATCTTCAAAACCTTCAAAGTCAGAAAGAGAGAAGGAG TCCAAAAAAGGAAAAGGAAAGAATTGGAAGAAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG211679 representing NM_011258
Red=Cloning site Green=Tags(s) MDIRKFFGVISSGKKPVNETVKNEKTKASEGTVKGKKGVKEAKVNNSGKEDASKPKQHSKKKRIINDSDS ESEETVQVKNAKKKSEKLSLSYKPGKVSQKDPVTYVSETDEDDDFVCKKAASKSKENGVSTNSYLGTSNV KKNEENVKTKNKPLSPIKLTPTSVLDYFGTESVQRSGKKMVTSKRKESSQNTEDSRLNDEAIAKQLQLDE DAELERQLHEDEEFARTLALLDEEPKIKKARKDSEEGEESFSSVQDDLSKAEKQKSPNKAELFSTARKTY SPAKHGKGRASEDAKQPCKSAHRKEACSSPKASAKLALMKAKEESSYNETELLAARRKESATEPKGEKTT PKKTKVSPTKRESVSPEDSEKKRTNYQAYRSYLNREGPKALGSKEIPKGAENCLEGLTFVITGVLESIER DEAKSLIERYGGKVTGNVSKKTNYLVMGRDSGQSKSDKAAALGTKILDEDGLLDLIRTMPGKRSKYEMAA EAEMKKEKSKLERTPQKNDQGKRKISPAKKESESKKCKLTLLKNSPMKAVKKEASTCPRGLDVKETHGNR SSNKEECLLWVDKYKPASLKNIIGQQGDQSCANKLLRWLRNWHKSSPEEKKHAAKFGKLASKDDGSSFKA ALLSGPPGVGKTTTASLVCQELGYSYVELNASDTRSKNSLKAVVAESLNNTSIKGFYTSGAAPSVSARHA LIMDEVDGMAGNEDRGGIQELIGLIKHTKIPIICMCNDRNHPKIRSLVHYCFDLRFQRPRVEQIKSAMLS IAFKEGLKIPPPAMNEIILGANQDVRQVLHNLSMWCAQSKALTYDQAKADSQRAKKDIRLGPFDVTRKVF AAGEETAHMSLMDKSDLFFHDYSIAPLFVQENYLHVKPVAAGGDMKKHLMLLSRAADSICDGDLVDNQIR SKQNWSLLPTQAIYASVLPGELMRGYMTQFPSFPSWLGKHSSTGKHDRIVQDLSLHMSLRTYSSKRTVNM DYLSHIRDALVRPLTSQGVEGAQHVIKLMDTYYLMKEDFENIMEVSSWGGKPSAFSKLDPKVKAAFTRAY NKEAHLTPYSLQVVKTSRLSTGPALDSEYSEEFQEDDTQSEKEQDAVETDAMIKKKTRSSKPSKSEREKE SKKGKGKNWKK TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_011258 |
ORF Size | 3393 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_011258.2, NP_035388.2 |
RefSeq Size | 4731 bp |
RefSeq ORF | 3396 bp |
Locus ID | 19687 |
UniProt ID | P35601 |
Gene Summary | The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins PCNA and activator 1. This subunit binds to the primer-template junction.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC223672 | Rfc1 (untagged) - Mouse replication factor C (activator 1) 1 (Rfc1), (10ug) |
CNY 14,540.00 |
|
MR211679 | Rfc1 (Myc-DDK-tagged) - Mouse replication factor C (activator 1) 1 (Rfc1) |
CNY 7,872.00 |
|
MR211679L3 | Lenti ORF clone of Rfc1 (Myc-DDK-tagged) - Mouse replication factor C (activator 1) 1 (Rfc1) |
CNY 11,780.00 |
|
MR211679L4 | Lenti ORF clone of Rfc1 (mGFP-tagged) - Mouse replication factor C (activator 1) 1 (Rfc1) |
CNY 11,780.00 |