Pkp4 (BC082578) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	5031422I09Rik; 9430019K17Rik; Armrp; p0071
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG211706 representing BC082578 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCGCCCCTGAACAGGGCTCACTGGTGGAGGAGGGGCAACCACAGACCCACCAGGAAGCTGTCTCCA CTGGCCCAGGCATGGAACCCGAGACCACAGCCACCACTATTCTAGCTTCCGTGAAGGAGCAGGAGCTTCA GTTTCAACGACTCACCCGAGAACTGGAAGTGGAAAGGCAGATTGTTGCCAGTCAGCTAGAAAGATGTAGG CTTGGAGCAGAATCGCCAAGCATCGCCAGCACCAGCTCAACTGAAAAGTCATTTCCTTGGAGATCAACAG ATGTGCCAAATCCTGGTGTGAGCAAACCTAGAGTTTCTGACACTATTCATCCCAACAACTATCTCATCAG GACAGAGCCAGAACAGGGGACCCTCTACTCACCAGAACAGACTTCTCTCCATGAAAGTGAGGGATCGTTG GGAAACTCAAGAAGTTCAACACAGATGAATTCTTACTCTGACAGTGGGTACCAGGAAGCAGGGAGTTTCC ACAACAGCCAAACCGTGAACAAGGCAGACAGCAGACAGCATCCATTCACAGGATCAACAAGTAACCATGT GGTGAGGACTTCAAGAGCTGAAGGACAAACACTGGTTCAGCCATCAGTAGCCAATCGGGCCATGCGGAGA GTTAGTTCAGTTCCATCTAGAGCACAGTCTCCTTCTTATGTTACCAGCACAGGCGTGTCTCCTTCAAGGG GGTCACTGAGAACTTCTCTGGGTAGTGGATTTGGCTCTCCATCAGTGACGGACTCCCGACCTCTGAACCC CAGTGCTTACTCCTCCAGCACGCTCCCTGCACAGCGGGCCGCCTCTCCATACTCACAGAGACCCGCCTCC CCAACAGCCGTGCGCCGGGTCGGGTCTGTCACCTCCCGGCAGACCTCCAATCCCAACGGACCAGTCCCTC AGTACCAGACCACCACCAGGGTGGGGTCCCCACTGACCCTGACTGATGCCCAGACTCGAGTAGCTTCCCC ATCCCAAGGCCAGGTGGGGTCATCGTCCCCGAAACGCTCTGGGATGACCGCCGTACCACAGCACCTGGGA CCTTCACTGCAAAGGACTGTTCATGACATGGACCAATTTGGACAGCAGCAGTATGACATTTATGAAAGGA TGGTTCCACCTCGGCCAGACAGCCTGACAGGCTTACGGAGTTCCTATGCCAGTCAGCATAGTCAGCTTGG GCAAGAGCTCCGTTCAGCTGTGTCTCCTGACTTGCACATCACTCCTATCTATGAAGGGAGGACCTACTAC AGTCCAGTGTACCGCAGCCCAAACCACGGGACCGTGGAGCTCCAGGGGTCACAGACAGCATTGTATCGCA CAGGCTCAGTAGGTATTGGAAATCTACAAAGGACATCCAGCCAGCGAAGCACCCTTACATACCAAAGAAA TAACTATGCCCTGAACACAGCAGCTACCTATGCGGAGCCCTACAGGCCAGTTCAGTACCGAGTCCAAGAG TGCAGCTATAACAGGCTGCAGCACACAGGGCCAGCTGATGACGGTGCCACAAGATCCCCATCAATAGACA GCATTCAGAAGGACCCCAGGGAGTTTGCCTGGCGTGACCCTGAGCTGCCAGAAGTCATTCACATGCTGCA GCACCAGTTCCCATCGGTCCAGGCAAATGCCGCAGCCTACCTGCAGCATCTGTGCTTTGGTGACAACAAA GTGAAGATGGAGGTGTACAGGCTAGGGGGAATCAAGCATCTGGTCGACCTGTTGGACCACAGGGTGTTGG AAGTTCAGAAGAATGCTTGTGGCGCCCTTCGAAACCTTGTGTTTGGCAAGTCAACAGACGAAAATAAAAT AGCAATGAAGAACGTTGGTGGAATCCCTGCCTTGCTGCGACTGTTAAGAAAATCCATTGATGCAGAAGTA AGGGAGCTTGTTACAGGGGTTCTTTGGAATCTATCTTCGTGTGATGCTGTAAAGATGACAATCATTCGAG ACGCGCTCTCAACCTTAACCAACACTGTGATAGTTCCACATTCTGGTTGGAATAACTCTTCCTTTGATGA TGATCATAAAATTAAATTTCAGACCTCACTGGTTCTTCGGAACACAACAGGTTGCCTGAGGAACCTCAGC TCTGCCGGGGAGGAAGCACGAAAACAAATGCGCTCCTGCGAGGGGCTGGTGGACTCGCTGTTGTATGTGA TCCACACGTGTGTGAACACTTCTGACTACGACAGCAAGACAGTGGAGAACTGTGTGTGTACCCTGAGGAA CCTGTCATACCGGCTGGAGCTGGAAGTCCCTCAGGCCCGGCTGCTCGGACTGAACGAGCTAGATGACTTA CTAGGAAAAGAGTCTCCCAGCAAAGACTCGGAACCAAGCTGCTGGGGAAAGAAGAAGAAAAAGAAAAAGA GAACGCCACAAGAGGACCAATGGGATGGAGTCGGGCCTATCCCTGGACTGTCCAAGTCCCCCAAGGGAGT CGAGATGCTGTGGCATCCATCAGTGGTAAAGCCGTACCTGACTCTGTTAGCAGAAAGTTCCAACCCTGCC ACCTTGGAAGGCTCTGCCGGATCCCTGCAGAACCTCTCTGCTGGCAACTGGAAGTTTGCAGCATACATCC GGGCAGCTGTCCGGAAGGAAAAAGGGCTTCCTATCCTTGTCGAGCTTCTAAGGATGGATAATGACAGAGT TGTCTCTTCTGTGGCCACTGCCTTGAGGAACATGGCGCTGGACGTTCGCAACAAGGAGCTCATAGGTAAA TACGCCATGCGAGACCTGGTGAACAGGCTTCCAGGTGGCAACGGCCCCAGCATCCTGTCCGATGAAACCG TGGCAGCCATCTGCTGTGCTCTGCACGAGGTCACCAGCAAAAACATGGAGAATGCCAAAGCTCTGGCCGA CTCGGGAGGTATAGAGAAGCTGGTGAACATAACCAAAGGCAGAGGAGACAGATCCTCTCTGAAAGTGGTG AAGGCGGCAGCCCAGGTCTTGAATACATTATGGCAGTACCGGGACCTCCGGAGCATTTACAAAAAGGATG GGTGGAATCAGAACCATTTTATTACACCTGTGTCCACGCTAGAGCGAGACCGATTCAAATCACACCCTTC TCTGTCCACCACCAACCAACAGATGTCGCCCATCATTCAGTCAGGCTCCAGCAAACCTTCACCAATATAC ATCAGTTCCTATTCCTCACCAGCAAGAGAACAAAATAGACGGCTACAGCATCAGCAGCTGTACTATCAAG ATGACTCCACCAGGAAGACCTTGGATGCATACAGACTGTACCTGCAGTCTCCTCGAAGCTATGAAGACCC TTATTGTGATGACCGAGTTCACTTCCCAGCTTCTACTGATTACTCAACGCAGTACGGACTGAAATCAACC ACGAATTATGTAGACTTTTATTCCACTAAACGACCTTCTTATAGAGCCGAACAGTACCCTGGGTCCCCGG ACTCCTGGGTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG211706 representing BC082578 Red=Cloning site Green=Tags(s) MPAPEQGSLVEEGQPQTHQEAVSTGPGMEPETTATTILASVKEQELQFQRLTRELEVERQIVASQLERCR LGAESPSIASTSSTEKSFPWRSTDVPNPGVSKPRVSDTIHPNNYLIRTEPEQGTLYSPEQTSLHESEGSL GNSRSSTQMNSYSDSGYQEAGSFHNSQTVNKADSRQHPFTGSTSNHVVRTSRAEGQTLVQPSVANRAMRR VSSVPSRAQSPSYVTSTGVSPSRGSLRTSLGSGFGSPSVTDSRPLNPSAYSSSTLPAQRAASPYSQRPAS PTAVRRVGSVTSRQTSNPNGPVPQYQTTTRVGSPLTLTDAQTRVASPSQGQVGSSSPKRSGMTAVPQHLG PSLQRTVHDMDQFGQQQYDIYERMVPPRPDSLTGLRSSYASQHSQLGQELRSAVSPDLHITPIYEGRTYY SPVYRSPNHGTVELQGSQTALYRTGSVGIGNLQRTSSQRSTLTYQRNNYALNTAATYAEPYRPVQYRVQE CSYNRLQHTGPADDGATRSPSIDSIQKDPREFAWRDPELPEVIHMLQHQFPSVQANAAAYLQHLCFGDNK VKMEVYRLGGIKHLVDLLDHRVLEVQKNACGALRNLVFGKSTDENKIAMKNVGGIPALLRLLRKSIDAEV RELVTGVLWNLSSCDAVKMTIIRDALSTLTNTVIVPHSGWNNSSFDDDHKIKFQTSLVLRNTTGCLRNLS SAGEEARKQMRSCEGLVDSLLYVIHTCVNTSDYDSKTVENCVCTLRNLSYRLELEVPQARLLGLNELDDL LGKESPSKDSEPSCWGKKKKKKKRTPQEDQWDGVGPIPGLSKSPKGVEMLWHPSVVKPYLTLLAESSNPA TLEGSAGSLQNLSAGNWKFAAYIRAAVRKEKGLPILVELLRMDNDRVVSSVATALRNMALDVRNKELIGK YAMRDLVNRLPGGNGPSILSDETVAAICCALHEVTSKNMENAKALADSGGIEKLVNITKGRGDRSSLKVV KAAAQVLNTLWQYRDLRSIYKKDGWNQNHFITPVSTLERDRFKSHPSLSTTNQQMSPIIQSGSSKPSPIY ISSYSSPAREQNRRLQHQQLYYQDDSTRKTLDAYRLYLQSPRSYEDPYCDDRVHFPASTDYSTQYGLKST TNYVDFYSTKRPSYRAEQYPGSPDSWV TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC082578
ORF Size	3443 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC082578, AAH82578
RefSeq Size	4331 bp
RefSeq ORF	3443 bp
Locus ID	227937
Gene Summary	Plays a role as a regulator of Rho activity during cytokinesis. May play a role in junctional plaques (By similarity).[UniProtKB/Swiss-Prot Function]

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