Dhx37 (BC066077) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	MGC90028, mKIAA1517
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG211713 representing BC066077 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGAAGCTGCGACGCCGCTACAACGTGAAGGGGCGGCTGCAGGCCGATCCCCGGCCCGCCAAGGGCC CTGAAGCGCCTCCCGTACGGCTGGAACTGGAGGGCAAGGATGTGTTGAAAGGAGTTGATGAAAGCAATGT TCTGGTGCTGCCTGGGAAGAGAAAGAAGAAGACCAAGGCGCCGCCCCCATCCAAGAAAGAGAGGAAGCCC CTGACAAAGAAGGAGAGGAAGGTGCTACAGAAGGTCTTAGAACAGAAGGAGAAGAAGAGCCAGCGTGCCG AACTGCTGCAGAAACTGAGTGAAGTCCAAGTATCGGAGGCCGAGATGAGTCTCTTCTACACCACAGCCAA GCTCGGCACTGGGGACCGCATGTATCATATCAAAGAGAGGAAGTCTGGCCAGCCTGCTGCCCGGGGCCAG GAGAAGGTCAGTAGCCTCAGCGGGGCCCACCGGAAGCGCCACAGGTCATCATCAACAGAGGAAGACTCTG AGTCTTCAGAAGATTCAGAGCCTGAGGAAGCCACAGCTGACCAACCTAGGACCAGCACAGGGACAGAGCC AGTGCATCCGCCACCAGCTCCTTCTGGGATCAACAGCCCTGCACCCACCCCGCAGCCCCCTCCATCTGGG ATCTCTGCACCTCCAAAAACACCAGCCTCTGCCCCGCCTCCACCCGTGGCCAAGCCTGCTGTATTCATCC CCGTGAACCGCACCCCTGAAATGCAGGAGGAGCGGCTCAAGCTCCCGATCCTCGCCGAGGAGCAAGCCAT CATGGAGGCGGTGGCTGAGCACCCCATTGTCATTGTGTGTGGCGAGACTGGCAGTGGGAAGACTACACAG GTGCCCCAGTTTCTCTACGAAGCAGGCTACAGCAGTGAGGACAGCATCATCGGTGTCACAGAACCCCGCC GAGTGGCCGCAGTAGCCATGTCCCAGCGGGTGGCCAAGGAGATGAACCTGTCACATCGGGTTGTCTCCTA CCAGATCCGCTACGAAGGGAATGTGACAGAAGAAACCAGGATCAAGTTCATGACGGACGGTGTGCTGCTC AAAGAGATCCAGAAGGACTTCCTGCTGCTGAAATACAAGGTGGTGATCATTGACGAAGCCCATGAGCGGA GCGTCTACACAGACATCCTTCTAGGGCTCCTGTCCCGCATTGTGGCTCTCCGGGCCAAGAGGCACCTGCC CCTCAAGCTGCTCATCATGTCGGCCACACTGCGGGTGGAGGACTTCACCCAGAACCAGCGGCTCTTCACC ACCCCTCCCCCGGTCATCAAGGTTGAGTCGAGGCAGTTCCCTGTGACTGTACATTTCAACAAGCGGACCC CATTGGATGACTACAGTGGTGAATGCTTCAGGAAGGTCTGCAAGATCCACCGGATGCTGCCTGCAGGTGG CATCCTGGTATTTCTCACAGGGCAGGCCGAGGTGCATGCCCTTTGCCGTCGGCTTAGGAAGGCCTTCCCC TTTCGTTGCTCCCAGCCACAAGAAAAGGAAGAGGACTCAGCAGAAGGGATGCGGAGGTTTAAGAAGTCCC GGACAAGGGCTAGGAAGGCCCAGGCCATGGCCTTGCCCCAGATCAACCTGGACAATTACTCTGTGCTGCC AGCCGGCGAAGGTGATGAGGACAGGGAGGCAGAGATGGACGATGAAGAGGAGGCCCTGGGCTCTGACCTA GATCTGGACCTGGGTGACAGTGAGGCAAATGAAGGTGAGCAGCCGGATGCCTCCCTGCCCCTTCACGTGC TCCCGCTCTACTCTTTGCTGGCCCCTGAGAAGCAGGCACAGGTCTTCAAACCTCCTCCCGAGGGGACGAG GCTGTGCGTTGTGGCCACCAATGTGGCTGAAACCTCCCTCACCATCCCTGGTATCAAGTATGTGGTGGAC TGTGGGAAAGTCAAAAAGCGCTATTATGATCGTGTCACAGGCGTGTCTTCCTTCCGTGTCACCTGGGTCT CCCAAGCATCCGCTGACCAGCGGGCTGGTCGTGCAGGCCGCACAGAGCCAGGCCACTGCTATAGGCTGTA TTCCTCAGCTGTTTTCGGGGACTTTGAGCAGTTTCCTCCTCCAGAGATCACCCGCAGACCAGTGGAGGAC TTGATCCTGCAAATGAAAGCACTCAGCATTGAGAAGGTCATCAACTTCCCGTTCCCCACCCCGCCGTCCG TGGAGGCCCTCGTTGCTGCAGAGGAGCTGCTGGTTGCCCTGGGGGCCCTGCAGGCACCTCCAAAACAGGA AAGAATGAAGAAGCTACAGATGTCACAGCTGAGCTGCCCCATCACCGCGCTGGGGCGGACCATGTCCACC TTCCCTGTGGCACCCCGCTATGCCAAGATGCTGGCCCTGAGCCAGCAGCATGGCTGCCTGCCCTACACCA TTGCCATCGTGGCTGCCATGACTGTGCGTGAGCTGTTTGAGGAGCTGGACAGACCTGCTGCCAGTGAAAA GGAGCTTGCCGAGCTAAAGGGCCGCCGAGCCCGTGTGGCCCAGATGAAGAGGACCTGGGCCGGGCAGGGG CCATCTCTGAAGCTTGGCGACCTCATGGTGTTGCTGGGTGCCGTGGGAGCCTGTGAGTACGCTGGCTGCT CACCCCAGTTCTGCCAAGCTAACGGACTGCGCTACAAGGCCATGCTTGAGATCCGGCGCCTGCGTGGCCA GCTGACCACTGCAGTCAACGCAGTGTGCCCCGAGGCTGGGCTTTTCCTGGACCCCAAAATGCAGCCTCCT ACTGAGAGCCAGGTGACTTACCTGCGGCAAATCATGGCAGCTGGTCTGGGTGACCACCTGGCCCGCCGGG TTCAGAGTGAGGACTTGCTGGACCCCAAGTGGAAGAATGCCTACAAGACTCCTCTTCTAGATGACCCCGT CTTCATCCACCCCAGCTCTGTACTCTTCAAAGAGTTGCCTGAGTTCGTGGTCTACCAGGAAATTGTGGAG ACAACCAAGATGTACATGAAAGGTGTCTCCACCGTGGAAATCCAGTGGATCCCCTCCCTGCTGCCTTCCT ACTGCCAGTTTGATGCACCCCTGGAGGAGCCAGCGCCCAGTTACTGCCCAGAGTCAGGACAGGTGTTGTG CCACCGGGCCAGCGTGTTCTACCGTGTGGGCTGGCCTCTCCCTGCCGTCCAGGTGGACTTCCCTGAAGGC ATTGACCGCTACAAATACTTTGCAAAGTTTCTGCTGGAGGGACAGGTCTTCCGCAAGCTGGCTTCATTCA AGAGCTGCCTGCTATCTAGCCCCAGCACCATGCTGAAGACCTGGGCCAGGCTGCAGCCCAGGACAGAGAC CCTATTGAGAGCCCTCGTGGCCCACAAGGCTGATTCTCGTGATTCCCTGCTGGCTGCATGGAAGAAGAAC CCCAAATACCTGCTGGCTGAGTACTGCGAGTGGCTCCCAAAGGCTATGCACAGCGATGTTGAGAAGAACT GGCCCCCTACCACTGACAGT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG211713 representing BC066077 Red=Cloning site Green=Tags(s) MGKLRRRYNVKGRLQADPRPAKGPEAPPVRLELEGKDVLKGVDESNVLVLPGKRKKKTKAPPPSKKERKP LTKKERKVLQKVLEQKEKKSQRAELLQKLSEVQVSEAEMSLFYTTAKLGTGDRMYHIKERKSGQPAARGQ EKVSSLSGAHRKRHRSSSTEEDSESSEDSEPEEATADQPRTSTGTEPVHPPPAPSGINSPAPTPQPPPSG ISAPPKTPASAPPPPVAKPAVFIPVNRTPEMQEERLKLPILAEEQAIMEAVAEHPIVIVCGETGSGKTTQ VPQFLYEAGYSSEDSIIGVTEPRRVAAVAMSQRVAKEMNLSHRVVSYQIRYEGNVTEETRIKFMTDGVLL KEIQKDFLLLKYKVVIIDEAHERSVYTDILLGLLSRIVALRAKRHLPLKLLIMSATLRVEDFTQNQRLFT TPPPVIKVESRQFPVTVHFNKRTPLDDYSGECFRKVCKIHRMLPAGGILVFLTGQAEVHALCRRLRKAFP FRCSQPQEKEEDSAEGMRRFKKSRTRARKAQAMALPQINLDNYSVLPAGEGDEDREAEMDDEEEALGSDL DLDLGDSEANEGEQPDASLPLHVLPLYSLLAPEKQAQVFKPPPEGTRLCVVATNVAETSLTIPGIKYVVD CGKVKKRYYDRVTGVSSFRVTWVSQASADQRAGRAGRTEPGHCYRLYSSAVFGDFEQFPPPEITRRPVED LILQMKALSIEKVINFPFPTPPSVEALVAAEELLVALGALQAPPKQERMKKLQMSQLSCPITALGRTMST FPVAPRYAKMLALSQQHGCLPYTIAIVAAMTVRELFEELDRPAASEKELAELKGRRARVAQMKRTWAGQG PSLKLGDLMVLLGAVGACEYAGCSPQFCQANGLRYKAMLEIRRLRGQLTTAVNAVCPEAGLFLDPKMQPP TESQVTYLRQIMAAGLGDHLARRVQSEDLLDPKWKNAYKTPLLDDPVFIHPSSVLFKELPEFVVYQEIVE TTKMYMKGVSTVEIQWIPSLLPSYCQFDAPLEEPAPSYCPESGQVLCHRASVFYRVGWPLPAVQVDFPEG IDRYKYFAKFLLEGQVFRKLASFKSCLLSSPSTMLKTWARLQPRTETLLRALVAHKADSRDSLLAAWKKN PKYLLAEYCEWLPKAMHSDVEKNWPPTTDS TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC066077
ORF Size	3452 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC066077, AAH66077
RefSeq Size	4235 bp
RefSeq ORF	3452 bp
Locus ID	208144

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