Rtel1 (BC105578) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	AI451565; AW540478; Rtel
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG211800 representing BC105578 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCAGGGTAGTCCTGAATGGTGTGACAGTGGATTTTCCTTTCCAGCCCTACCCATGCCAACAGGAAT ATATGACCAAGGTGCTAGAATGTCTCCAGAAGAAAGTGAATGGCATCCTGGAGAGCCCCACAGGCACTGG GAAGACGCTGTGCCTCCTCTGTTCCACCTTGGCCTGGCAACAACATCTCCGAGATGCAGTTTCTTCCCTA AAGATTGCTGAGAGAGTTCAAGGGGAACTCTTTGCCAGTCGGACCTTGTCATCCTGGGGGAGTGCTGCTG CCGCCAGCGGAGACTCAATAGAGTGCTACACAGATATCCCAAAGATCATCTATGCTTCTAGAACGCACTC CCAGCTAACTCAGGTCATCCGTGAGCTTCGGAATACCGCCTACCGGCCCAAGGTATGTGTGCTGGGCTCC CGGGAGCAGCTGTGTATTCATCCTGAAGTGAAGAAGCAGGAGAGTAATCACATGCAGATCAGTTTGTGCC GCAAGAAGGTAGCAAGTCGCTCCTGTCATTTCTACAACAATGTGGAAACGAAATTCCTGGAGCAAGATTT GGCTACCCCCATCCTGGATATTGAGGACCTTGTCAAGAATGGAAGCAAACAAAAAATGTGCCCATACTAC CTTTCTCGAAACATGAAACAGCAAGCGGACATCATCTTTATGCCATACAATTACCTGTTGGATGCTAAGA GTCGTAAGGCGCACAGCATTGACCTGAAGGGAACAGTTGTGATCTTTGATGAAGCTCACAATGTGGAGAA GATATGTGAGGAGTCAGCCTCCTTTGACTTGACTCCCCGTGATGTGGCTTCAGGACTGGAGATTATCAAC CAGGTTTTGGAGGAACAAGCCCGGGTGACTCAGCAGGGTGAACTCCAACAGGAGTTCATTGTAGACACGT CCAGCTCAGGACTCAACATGGAGCTGGAAGACATCGCAAAGCTAAAGATGATCCTGCTTCGCCTGGAGGA GGCTATTGATGCCGTTCAGCTGCCTGGGGATGACAGAGGCGTCACCAAACCTGGAAGCTATATCTTCGAG CTGTTTGCTGAAGCTCAAATAACATTTCAAACCAAAGGCTGCATTTTGGAATCACTGGACCAGATAATCC AGCACCTGGCAGGCCGTACTGGTGTGTCCACCAACACGGCTGGGTTGCAGAAGCTTATGGACATTATCCA GATTGTGTTCAGCGCGGACCCTCCGGAAGGCAGCCCTGGTTCTCTGGTGGGGCTGGGTATCTCACATTCC TATAAGGTACACATACACCCTGAAACCAGCCACCGGAGAGCAGCTAAGCGGTCAGATGCCTGGAGTACCA CTGCATCCAGGAAACAAGGGAAGGTGCTGAGCTATTGGTGCTTCAGTCCCAGCCAGAGTATGCGGGAACT GGTCTGCCAAGGAGTTCGTACCCTTATCCTCACCAGCGGTACCCTGGCTCCACTGTCTTCCTTTGCTCTG GAGATGCAGATTCCATTCCCAGTCTGTCTGGAGAATCCACACATCATTGACAAGAACCAGCTCTGGGTGG GGATTGTCCCCAGAGGCCCTGATGGTGTTCAGCTAAGCTCTGCCTATGACAAAAGGTTTTCTGAAGAGTG TTTATCTTCCCTGGGGAAGGCTCTGAGCAACATTGCTCGCGTGGTGCCCCATGGGCTTCTGGTCTTCTTC CCTTCCTACCCTGTCATGGAGAAAAGCCTGGAGTTCTGGCAGGTACAAGGATTGGCCAGGAAGGTAGAGG CACTAAAGCCTCTGTTCGTGGAACCCAGGAACAAAGGCAGCTTCTCAGAGGTCATTGATGCCTACTACCA ACAAGTTGCCTCCCCTGCGTCTAATGGGGCCACCCTCTTAGCAGTGTGTCGGGGAAAGGCCAGCGAAGGG CTGGACTCCTCAGACATGAATGGTCGTGGTGTGATTGTCACGGGCCTCCCATATCCTCCACGCATGGATC CCCGTGTTGTCCTCAAGATGCAGTTCTTGGATGAGATGAGAGGCCGGAGTGGGGTTGGAGGCCAGTGCCT CTCTGGGCAGGAATGGTACCAGCAGCAAGCATCCAGGGCTGTGAACCAGGCTATTGGGAGGGTTATTCGA CACCGCCATGACTATGGGGCCATCTTCCTGTGTGACCACAGGTTCGCCTATGCTGATGCCAGGGCCCAGC TGCCCTCCTGGGTGCGCCCCTACCTTAAGGTGTATGACAACTTTGGCCATGTCATCCGAGATGTGGCCCA GTTCTTCCGTGTTGCTCAGAAAACTATGCCTTTGCCAGTTCCTCAGGCTGTGACCTCAAGTGTGAGTGAG GGAGAAATTGCTCTCAAGGATGCTACATTGTCCAGCTACTCCCTCTCTACCAGGAAAGCCATGAGTTTGG ATGTGCGTGTGCCCAGCCTGAGGCAGAAGCCCATAGGATTACCAGCTGCTGGAGACTCTGAGAGCAGCCT GTGTGGGGAGTATGAGCAGCAGACATTTTCTGCCCAGCAGAGACCTATGGGACTGCTAGCTGCCTTAGAA TACAACGAGCAGAAGGCTGGGGCATCTGAGGAGCAGGCACTGGGCTCCTCCACCCCATCTCTCCGGTGCG AGAAGAGGCTGTCTACTGAGCAAAAAGGAGGAAGGAAGAAAGTCAGGCTGGTCAACCATCCGGAGGAACC AATGGCTGGCACACAGGCAGGCAGAGCCAAGATGTTCATGGTGGCTGTGAAGCAAGCACTGAGCCAAGCT AACTTTGACACCTTTACCCAGGCCCTGCAGCACTATAAGAGTTCTGATGACTTTGAAGCCCTAGTGGCCT CTCTCACCTGTCTCTTTGCTGAAGACCCCAAGAAACACACCCTGCTTAAAGGTTTCTACCAGTTTGTTCG ACCCCACCACAAGCAGCAGTTTGAGGACATCTGCTTCCAGCTAACAGGCCAACGATGTGGCTACCAGCCA GGAAAGAGAGAGCTGGAGTCTAAACTGACCTTGTCTGAAGGTGTAGACAGGCAGCTGGATCCTGGACAGC ACCTGAACCAGGGGCAGCCTCACCTGTCTGCCCATCCAACCTCCAAAGGACACACCAGCCATTGTACAAA AGTGGGATGTGCAGTAGAAAAACCTGGCCAGCCTGCTGTGAGTGACTACCTATCTGATGTCCACAAGGCT CTGGGATCTGCAAGCTGCAACCAGCTTACGGCAGCTCTGAGGGCATACAAACAGGATGATGACCTGGACA AGGTGGTGGCTGTGGTGGCAGCACTGACCACTGCAAAACCTGAACACTTACCCTTGCTACAGAGATTTGG CATGTTTGTGCGTCGGCATCATAAGCCTCAGTTCCTACAGACCTGTGCAGACCTAATGGGCCTGCCTACC ACAGGCAAGGACTTGGAGCTCGAAGGTCCCAGAGATGAGAGCCCAACTGTGCCTCCTGAGCTTACCCATG AGGACCTGAAACCAGGGCCCTCGATGTCCAAGAAACCTGAGAAGACCCAGAGTAAGATCTCATCCTTCTT TAGACAGAGGCCAGATGAGAGTGTGAGGTCTGATGATACCACCCCAAAGCCCATGCAACTTCCTCCTAGA CTACCCCATGAGCTTATGAAGCCTCATCGGAGTAAGCAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG211800 representing BC105578 Red=Cloning site Green=Tags(s) MPRVVLNGVTVDFPFQPYPCQQEYMTKVLECLQKKVNGILESPTGTGKTLCLLCSTLAWQQHLRDAVSSL KIAERVQGELFASRTLSSWGSAAAASGDSIECYTDIPKIIYASRTHSQLTQVIRELRNTAYRPKVCVLGS REQLCIHPEVKKQESNHMQISLCRKKVASRSCHFYNNVETKFLEQDLATPILDIEDLVKNGSKQKMCPYY LSRNMKQQADIIFMPYNYLLDAKSRKAHSIDLKGTVVIFDEAHNVEKICEESASFDLTPRDVASGLEIIN QVLEEQARVTQQGELQQEFIVDTSSSGLNMELEDIAKLKMILLRLEEAIDAVQLPGDDRGVTKPGSYIFE LFAEAQITFQTKGCILESLDQIIQHLAGRTGVSTNTAGLQKLMDIIQIVFSADPPEGSPGSLVGLGISHS YKVHIHPETSHRRAAKRSDAWSTTASRKQGKVLSYWCFSPSQSMRELVCQGVRTLILTSGTLAPLSSFAL EMQIPFPVCLENPHIIDKNQLWVGIVPRGPDGVQLSSAYDKRFSEECLSSLGKALSNIARVVPHGLLVFF PSYPVMEKSLEFWQVQGLARKVEALKPLFVEPRNKGSFSEVIDAYYQQVASPASNGATLLAVCRGKASEG LDSSDMNGRGVIVTGLPYPPRMDPRVVLKMQFLDEMRGRSGVGGQCLSGQEWYQQQASRAVNQAIGRVIR HRHDYGAIFLCDHRFAYADARAQLPSWVRPYLKVYDNFGHVIRDVAQFFRVAQKTMPLPVPQAVTSSVSE GEIALKDATLSSYSLSTRKAMSLDVRVPSLRQKPIGLPAAGDSESSLCGEYEQQTFSAQQRPMGLLAALE YNEQKAGASEEQALGSSTPSLRCEKRLSTEQKGGRKKVRLVNHPEEPMAGTQAGRAKMFMVAVKQALSQA NFDTFTQALQHYKSSDDFEALVASLTCLFAEDPKKHTLLKGFYQFVRPHHKQQFEDICFQLTGQRCGYQP GKRELESKLTLSEGVDRQLDPGQHLNQGQPHLSAHPTSKGHTSHCTKVGCAVEKPGQPAVSDYLSDVHKA LGSASCNQLTAALRAYKQDDDLDKVVAVVAALTTAKPEHLPLLQRFGMFVRRHHKPQFLQTCADLMGLPT TGKDLELEGPRDESPTVPPELTHEDLKPGPSMSKKPEKTQSKISSFFRQRPDESVRSDDTTPKPMQLPPR LPHELMKPHRSKQ TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC105578
ORF Size	3609 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC105578, AAI05579
RefSeq Size	4430 bp
RefSeq ORF	3611 bp
Locus ID	269400
Gene Summary	ATP-dependent DNA helicase implicated in telomere-length regulation, DNA repair and the maintenance of genomic stability. Acts as an anti-recombinase to counteract toxic recombination and limit crossover during meiosis. Regulates meiotic recombination and crossover homeostasis by physically dissociating strand invasion events and thereby promotes noncrossover repair by meiotic synthesis dependent strand annealing (SDSA) as well as disassembly of D loop recombination intermediates. Also disassembles T loops and prevents telomere fragility by counteracting telomeric G4-DNA structures, which together ensure the dynamics and stability of the telomere.[UniProtKB/Swiss-Prot Function]

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