Trpm4 (BC096475) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	TRPM4B, FLJ20041
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG211808 representing BC096475 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGGGGCCGGAGAAGGAGCAGAGCTGGATCCCTAAGATCTTCAGGAAGAAGGTGTGCACGACGTTCA TAGTGGACCTCTCCGATGATGCGGGAGGGACCTTGTGCCAGTGTGGGCAACCTCGGGATGCCCACCCTTC TGTGGCTGTGGAGGATGCCTTTGGGGCAGCCGTAGTTACCGAGTGGAACAGTGATGAGCACACCACGGAG AAGCCCACAGATGCCTATGGGGACCTGGACTTCACGTACTCTGGCCGAAAGCACAGCAACTTTCTCCGGC TGTCTGACCGCACGGATCCGGCCACTGTGTACAGTCTCGTTACCCGCTCTTGGGGCTTTCGTGCCCCAAA CTTGGTGGTGTCGGTGCTGGGGGGGTCGGGGGGCCCCGTGCTCCAGACCTGGCTGCAGGACCTTCTGCGT CGCGGGCTGGTGAGAGCCGCCCAGAGCACAGGGGCCTGGATCGTCACTGGGGGACTGCACACAGGCATTG GCCGGCACGTGGGTGTGGCTGTAAGGGACCACCAGACAGCCAGCACTGGGAGCAGCAAGGTGGTGGCCAT GGGTGTGGCCCCCTGGGGAGTGGTCCGGAACAGAGACATGCTGATCAACCCCAAGGGCTCATTCCCCGCA AGGTACCGGTGGCGTGGTGACCCTGAGGATGGTGTTGAGTTCCCCCTGGACTATAACTATTCTGCTTTCT TCTTGGTGGATGATGGCACCTATGGCCGCCTGGGTGGTGAGAACCGCTTCCGCCTTCGGTTTGAGTCCTA TGTGGCTCAGCAGAAGACCGGAGTGGGAGGGACTGGAATTGACATCCCAGTGCTCCTTCTTCTCATCGAT GGAGATGAGAAGATGTTAAAGCGGATAGAGGATGCCACCCAAGCTCAGCTCCCCTGCCTCTTGGTGGCGG GCTCTGGGGGTGCTGCAGACTGTCTGGTGGAGACCCTGGAAGATACTCTGGCCCCAGGGAGTGGGGGACT TAGGCGAGGCGAAGCCCGGGATCGGATTAGGCGATACTTCCCTAAAGGAGACCCTGAGGTTCTTCAGGCC CAGGTAGAGAGGATCATGACCCGAAAGGAGTTGCTGACGGTCTATTCATCAGAAGACGGCTCCGAGGAGT TTGAGACTATCGTTTTGAGGGCTCTTGTGAAAGCCTGTGGGAGCTCTGAGGCCTCAGCCTACCTGGATGA GCTGCGTTTGGCTGTGGCTTGGAACCGCGTGGACATCGCCCAAAGTGAACTTTTCCGTGGGGACATCCAA TGGCGGTCCTTCCACCTGGAGGCATCTCTCATGGATGCTCTGCTGAATGACCGGCCTGAATTTGTGCGCC TGCTCATCTCCCATGGCCTTAGCCTGGGACACTTCCTGACCCCCGTGCGTCTGGCCCAGCTGTACAGCGC GGTGTCCCCTAACTCACTGATCCGAAATCTTCTGGACCAGGCGTCCCATGCTAGTAGCAGCAAATCCCCT CCTGTAAATGGAACTGTAGAGCTCCGGCCTCCCAATGTGGGGCAAGTGCTGAGGACTCTGTTGGGAGAAA CGTGTGCACCACGGTACCCTGCCAGGAACACCCGAGACTCCTATCTGGGCCAGGACCACAGAGAGAATGA CTCTCTGCTTATGGACTGGGCCAACAAGCAACCATCAACGGATGCAAGCTTTGAACAAGCCCCCTGGAGT GACCTGCTTATTTGGGCTCTGTTGCTGAACCGGGCCCAGATGGCTATCTATTTCTGGGAGAAGGGCTCCA ACTCAGTGGCCTCTGCTCTTGGGGCTTGTCTTTTACTCCGAGTGATGGCTCGCCTAGAGTCGGAGGCTGA GGAGGCCGCGCGGCGGAAGGATCTTGCTGCCACGTTTGAAAGCATGAGCGTGGACCTCTTTGGAGAGTGT TACCACAACAGTGAAGAGCGAGCAGCCCGCCTTCTTCTGCGCCGCTGTCCCCTCTGGGGAGAGGCCACCT GCCTCCAGCTTGCCATGCAGGCTGATGCCCGAGCGTTCTTTGCCCAGGATGGAGTCCAGTCTCTTCTGAC ACAGAAGTGGTGGGGGGAGATGGACAGCACAACTCCCATCTGGGCCCTGCTTCTCGCCTTCTTCTGCCCG CCTCTCATCTACACCAACCTTATCGTCTTCAGGAAGTCAGAGGAGGAGCCCACACAGAAGGATCTTGACT TTGATATGGACAGCAGCATCAATGGAGCAGGTCCTCCTGGGACTGTAGAACCCTCTGCAAAGGTGGCCCT TGAGAGGCGGCAGCGGCGACGGCCAGGACGTGCCCTCTGCTGTGGCAAGTTCTCCAAGCGCTGGTCGGAC TTCTGGGGTGCCCCAGTGACTGCCTTCCTGGGTAATGTGGTCAGTTACCTGCTGTTCCTGCTGCTGTTTG CCCACGTGTTGCTGGTGGATTTCCAGCCCACAAAGCCAAGTGTCTCCGAGCTGCTGCTGTACTTCTGGGC CTTCACGTTGCTGTGCGAGGAGCTGCGCCAGGGCCTGGGTGGTGGCTGGGGTAGCCTGGCCAGTGGGGGA CGTGGTCCGGACAGAGCTCCCCTGCGCCATCGGCTGCACCTCTACCTCTCAGATACCTGGAACCAATGTG ACCTGCTGGCACTCACCTGCTTCCTGCTGGGCGTAGGCTGCCGGCTGACCCCTGGGCTCTTTGACTTGGG ACGCACCGTCCTCTGCCTTGACTTCATGATCTTCACACTGCGCCTGCTGCACATCTTCACGGTGAACAAG CAGCTGGGGCCCAAGATTGTCATAGTGAGCAAAATGATGAAGGATGTATTCTTCTTCCTCTTCTTCCTCT GCGTGTGGCTTGTAGCTTATGGGGTGGCCACAGAGGGGATCCTGAGGCCCCAGGACCGCAGTCTACCGAG TATCCTGCGCAGGGTCTTCTACCGACCGTATCTGCAGATCTTCGGACAAATTCCCCAGGAGGAAATGGAC GTGGCCCTCATGATTCCAGGTAACTGCTCAATGGAGCGGGGCTCCTGGGCTCATCCGGAGGGGCCCGTCG CAGGCTCCTGTGTGTCCCAGTATGCCAACTGGCTGGTGGTGTTGCTCCTTATCGTCTTCTTGCTGGTGGC CAATATCCTGTTGCTCAACCTGCTCATCGCCATGTTCAGCTACACCTTCAGCAAAGTGCACGGCAACAGC GACCTCTACTGGAAGGCACAGCGCTACAGCCTCATCCGGGAATTCCATTCGAGGCCTGCCCTGGCCCCAC CCCTCATCATCATCTCTCATGTGCGCCTCCTCATCAAGTGGCTGCGCAGGTGTCGCAGGTGTCGCAGGGC TAACTTGCCCGCCTCCCCAGTCTTCGAGCACTTCCGTGTCTGTCTCTCTAAGGAAGCAGAGCGGAAGCTG CTGACTTGGGAGTCTGTGCACAAAGAGAACTTCCTGTTGGCACAAGCTCGGGACAAGCGAGACAGTGACT CGGAGCGTTTGAAACGCACGTCTCAGAAGGTGGACACTGCACTGAAGCAGCTGGGACAGATCAGAGAGTA CGATCGGCGTCTGAGGGGGCTGGAGAGAGAGGTCCAACACTGTTCTCGAGTCCTGACTTGGATGGCTGAG GCCCTTAGCCACTCTGCCTTGCTGCCTCCAGGGGCCCCACCCCCTCCGAGCCCCACTGGTTCCAAAGAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG211808 representing BC096475 Red=Cloning site Green=Tags(s) MVGPEKEQSWIPKIFRKKVCTTFIVDLSDDAGGTLCQCGQPRDAHPSVAVEDAFGAAVVTEWNSDEHTTE KPTDAYGDLDFTYSGRKHSNFLRLSDRTDPATVYSLVTRSWGFRAPNLVVSVLGGSGGPVLQTWLQDLLR RGLVRAAQSTGAWIVTGGLHTGIGRHVGVAVRDHQTASTGSSKVVAMGVAPWGVVRNRDMLINPKGSFPA RYRWRGDPEDGVEFPLDYNYSAFFLVDDGTYGRLGGENRFRLRFESYVAQQKTGVGGTGIDIPVLLLLID GDEKMLKRIEDATQAQLPCLLVAGSGGAADCLVETLEDTLAPGSGGLRRGEARDRIRRYFPKGDPEVLQA QVERIMTRKELLTVYSSEDGSEEFETIVLRALVKACGSSEASAYLDELRLAVAWNRVDIAQSELFRGDIQ WRSFHLEASLMDALLNDRPEFVRLLISHGLSLGHFLTPVRLAQLYSAVSPNSLIRNLLDQASHASSSKSP PVNGTVELRPPNVGQVLRTLLGETCAPRYPARNTRDSYLGQDHRENDSLLMDWANKQPSTDASFEQAPWS DLLIWALLLNRAQMAIYFWEKGSNSVASALGACLLLRVMARLESEAEEAARRKDLAATFESMSVDLFGEC YHNSEERAARLLLRRCPLWGEATCLQLAMQADARAFFAQDGVQSLLTQKWWGEMDSTTPIWALLLAFFCP PLIYTNLIVFRKSEEEPTQKDLDFDMDSSINGAGPPGTVEPSAKVALERRQRRRPGRALCCGKFSKRWSD FWGAPVTAFLGNVVSYLLFLLLFAHVLLVDFQPTKPSVSELLLYFWAFTLLCEELRQGLGGGWGSLASGG RGPDRAPLRHRLHLYLSDTWNQCDLLALTCFLLGVGCRLTPGLFDLGRTVLCLDFMIFTLRLLHIFTVNK QLGPKIVIVSKMMKDVFFFLFFLCVWLVAYGVATEGILRPQDRSLPSILRRVFYRPYLQIFGQIPQEEMD VALMIPGNCSMERGSWAHPEGPVAGSCVSQYANWLVVLLLIVFLLVANILLLNLLIAMFSYTFSKVHGNS DLYWKAQRYSLIREFHSRPALAPPLIIISHVRLLIKWLRRCRRCRRANLPASPVFEHFRVCLSKEAERKL LTWESVHKENFLLAQARDKRDSDSERLKRTSQKVDTALKQLGQIREYDRRLRGLEREVQHCSRVLTWMAE ALSHSALLPPGAPPPPSPTGSKD TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC096475
ORF Size	3641 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC096475, AAH96475
RefSeq Size	4053 bp
RefSeq ORF	3641 bp
Locus ID	68667
Gene Summary	Calcium-activated non selective (CAN) cation channel that mediates membrane depolarization. While it is activated by increase in intracellular Ca(2+), it is impermeable to it (PubMed:17188667, PubMed:29211714). Mediates transport of monovalent cations (Na(+) > K(+) > Cs(+) > Li(+)), leading to depolarize the membrane. It thereby plays a central role in cadiomyocytes, neurons from entorhinal cortex, dorsal root and vomeronasal neurons, endocrine pancreas cells, kidney epithelial cells, cochlea hair cells etc. Participates in T-cell activation by modulating Ca(2+) oscillations after T lymphocyte activation, which is required for NFAT-dependent IL2 production. Involved in myogenic constriction of cerebral arteries. Controls insulin secretion in pancreatic beta-cells. May also be involved in pacemaking or could cause irregular electrical activity under conditions of Ca(2+) overload. Affects T-helper 1 (Th1) and T-helper 2 (Th2) cell motility and cytokine production through differential regulation of calcium signaling and NFATC1 localization. Enhances cell proliferation through up-regulation of the beta-catenin signaling pathway (By similarity). Essential for the migration but not the maturation of dendritic cells (PubMed:18758465).[UniProtKB/Swiss-Prot Function]

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