Zfp521 (BC021376) Mouse Tagged ORF Clone

CAT#: MG211913

  • TrueORF®

Zfp521 (tGFP-tagged) - Mouse zinc finger protein 521 (cDNA clone MGC:29358 IMAGE:5038671)



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Mouse Protein / Lysate Request

CNY 12,640.00


货期*
7周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms B930086A16Rik; Evi3; Znf521
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG211913 representing BC021376
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCTCGCCGCAAGCAAGCGAAACCGAGATCCCTCAAAGACCCCAACTGTAAACTTGAAGACAAGATTG
AAGATGGGGAGGCAGTAGACTGCAAGAAGAGGCCAGAAGACGGGGAGGAGTTGGAGGAGGACGCTGTGCA
CAGCTGTGACAGCTGCCTCCAGGTGTTTGAGTCACTGAGCGACATCACAGAGCACAAGATCCATCAGTGC
CAGCTGACAGATGGCGTTGACGTTGAGGACGACCCCAGCTGCTCTTGGCCAGCCTCCTCGCCTTCCAGTA
AGGACCAGACTTCCCCCAGCCATGGAGAAGGGTGTGATTTCGGAGAGGAAGAAGGTGGCCCTGGACTGCC
TTACCCATGCCAGTTCTGTGACAAGTCCTTTAGCCGCCTCAGCTACCTAAAGCACCATGAACAGAGCCAT
AGTGACAAGCTGCCCTTCAAGTGCACCTACTGCAGTCGGCTGTTCAAACACAAGCGGAGCCGTGACCGCC
ACATAAAGCTCCACACTGGGGATAAGAAGTATCACTGTAGCGAGTGTGACGCAGCCTTCTCCCGGAGCGA
TCATCTCAAGATCCACTTAAAGACTCACACCTCCAACAAGCCATATAAATGTGCCGTCTGCCGCAGAGGG
TTCCTCTCCTCTAGCTCCTTGCACGGACATATGCAGGTTCATGAGCGGAACAAGGACGGCTCCCAGTCTG
GCTCCAGGATGGAGGACTGGAAGATGAAGGACACTCAGAAGTGCAGCCAGTGCGAGGAAGGCTTTGACTT
CCCGGAAGACCTCCAGAAGCACATTGCAGAATGCCACCCCGAGTGCTCCCCTAATGAGGACCGAGCAGCC
CTCCAGTGCATGTACTGCCACGAGCTGTTTGTGGAGGAGACGTCCCTCATGAACCACATCGAGCAGGTGC
ACGGGGGTGAGAAGAAGAACTCTTGCAGCATTTGCTCAGAGAGCTTCCTCACGGTCGAGGAGCTGTATAG
CCACATGGACAGTCACCAGCAGCCAGAGTCCTGCAATCACAGCAACAGCCCTTCCCTGGTCACTGTGGGT
TACACCTCAGTGTCCAGCACGACTCCAGACTCGAACCTCTCAGTGGACAGCTCGACCATGGTAGAGGCTG
CACCACCGATTCCAAAGAGCCGCGGGAGGAAGCGAGCTGCTCAGCAGACCTCCGATATGACTGGCCCCTC
TAGTAAGCAGGCGAAAGTCACCTACAGCTGTATTTACTGCAACAAACAGTTATTTTCCAGTCTCGCGGTT
CTGCAGATTCACTTGAAAACTATGCATTTAGATAAGCCTGAGCAGGCTCATATCTGTCAGTATTGCTTGG
AGGTCTTACCCTCACTCTATAACCTAAATGAACATCTTAAACAAGTGCACGAAGCTCAGGACCCCGGCCT
GATTGTTTCGGCCATGCCTGCCATTGTTTACCAGTGCAACTTCTGTTCCGAAGTTGTCAATGACCTCAAC
ACCCTTCAGGAGCACATCCGATGTTCTCATGGGTTTGCCAATCCCGCGGCCAAGGACAGCAATGCGTTCT
TTTGTCCCCATTGTTACATGGGGTTTCTCACTGACTCTTCACTTGAAGAGCATATAAGACAGGTCCATTG
TGACCTCAGTGGCTCCCGGTTTGGGTCTCCTGTGCTTGGGACTCCAAAAGAACCGGTGGTTGAAGTCTAC
TCCTGTTCCTATTGTACAAATTCGCCAATATTCAACAGTGTTCTTAAACTGAATAAGCATATTAAAGAGA
ATCATAAAAACATTCCCTTGGCCCTGAATTATATTCACAATGGGAAGAAATCCCGGGCCTTGAGCCCTTT
GTCCCCTGTGGCTATAGAACAAACAACTCTTAAGATGATGCAGACTGTGGGAGGCGGGCCTGCCCGTGCC
TCTGGAGAGTATATCTGTAATCAGTGTGGTGCTAAGTACACGTCCCTAGACAGCTTTCAGACTCACCTCA
AAACCCATTTGGACACCGTGCTGCCAAAACTGACCTGCCCTCAGTGTAACAAAGAATTCCCCAACCAAGA
GTCCTTGCTAAAGCACGTGACCATCCACTTTATGATCACCTCAACCTACTACATCTGTGAGAGCTGTGAC
AAGCAGTTCACCTCCGTGGATGACCTCCAGAAGCACCTGCTGGACATGCACACCTTCGTCTTCTTCCGCT
GCACACTCTGCCAGGAAGTGTTCGACTCCAAGGTCTCTATCCAGCTGCACTTGGCCGTGAAGCACAGTAA
CGAGAAGAAGGTGTACCGCTGCACGTCCTGCAACTGGGACTTCCGCAACGAGACCGACCTGCAGCTGCAC
GTGAAGCACAACCACTTGGAGAACCAAGGCAAAGTGCACAAGTGCATCTTCTGCGGCGAGTCGTTTGGCA
CCGAGGTGGAGCTGCAGTGCCACATCACCACCCACAGCAAGAAGTACAACTGCAGGTTCTGCAGCAAGGC
CTTCCACGCCGTCATCCTGCTGGAGAAGCACCTGCGGGAGAAACACTGTGTGTTTGAAACCAAGACCCCC
AACTGTGGCACCAACGGGGCCTCGGAGCAAGTGCAGAAGGAGGAGGCTGAGCTGCAGACCTTGCTCACCA
ACAGCCAGGAGTCCCATAACAGCCATGATGGGAGTGAGGAGGACGTGGACAGCTCTGAACCCATGTATGG
CTGCGACATCTGCGGGGCGGCCTACACCATGGAGACGTTGCTGCAGAACCACCAGCTCCGAGACCACAAC
ATCAGGCCTGGAGAAAGCGCCATCGTGAAGAAGAAGGCGGAGCTCATTAAAGGGAATTACAAGTGTAACG
TTTGCTCGAGAACCTTCTTCTCCGAAAACGGGCTCCGTGAACACATGCAGACCCACTTAGGCCCGGTCAA
ACACTACATGTGCCCTATCTGTGGAGAGCGCTTCCCTTCCCTATTAACTCTCACAGAGCACAAAGTCACA
CACAGCAAGAGCCTGGATACCGGAAACTGTCGTATTTGTAAGATGCCCCTGCAGAGCGAAGAGGAGTTTT
TGGAGCACTGCCAGATGCACCCTGACCTGAGGAATTCCCTGACTGGGTTTCGTTGTGTGGTGTGTATGCA
GACTGTGACCTCCACCTTGGAACTCAAAATCCACGGGACCTTCCACATGCAGAAGACGGGGAATGGGTCC
TCCGTGCAGACCACCGGGCGTGGCCAGCACGTTCAGAAACTGTATAAGTGCGCATCTTGCCTCAAAGAGT
TCCGTTCCAAGCAAGATCTGGTGAAACTCGACATCAACGGCCTGCCATATGGTCTGTGTGCCGGCTGTGT
GAATCTCAGTAAGAGCAGTAGCCCGGGCCTCAGTCTCCCTCCTGGTGCCAGCAGGCCAGGCTTGGGCCAG
AATGAGAGTCTGAGCGCCATGGAAGGAAAAGGCAAGGCGGGGGGACTGAAGACGCGCTGTTCAAGCTGCA
ATGTCAAGTTTGAGTCTGAAAGCGAACTGCAGAACCACATCCAGACGGTGCACCGGGAGCTTGTGCCAGA
CGCCAACAGCACACAGTTGAAAACGCCCCAAGTGTCACCCATGCCCAGAATCAGTCCCTCCCAGTCCGAT
GAGAAGAAGACCTACCAGTGTATCAAGTGTCAGATGGTTTTCTACAACGAGTGGGACATCCAGGTTCATG
TGGCAAATCACATGATTGATGAAGGGCTGAACCATGAGTGCAAACTCTGCAGCCAGACCTTTGACTCCCC
TGCCAAACTTCAGTGTCACCTGATAGAGCACAGCTTCGAAGGGATGGGTGGTACCTTCAAGTGCCCCGTC
TGTTTCACAGTGTTTGTTCAAGCTAACAAGTTGCAACAGCATATATTCTCTGCCCATGGACAAGAAGACA
AGATCTATGACTGCACACAATGCCCACAGAAGTTTTTCTTCCAAACAGAGCTACAGAATCATACGATGAC
TCAGCACAGCAGT


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG211913 representing BC021376
Red=Cloning site Green=Tags(s)

MSRRKQAKPRSLKDPNCKLEDKIEDGEAVDCKKRPEDGEELEEDAVHSCDSCLQVFESLSDITEHKIHQC
QLTDGVDVEDDPSCSWPASSPSSKDQTSPSHGEGCDFGEEEGGPGLPYPCQFCDKSFSRLSYLKHHEQSH
SDKLPFKCTYCSRLFKHKRSRDRHIKLHTGDKKYHCSECDAAFSRSDHLKIHLKTHTSNKPYKCAVCRRG
FLSSSSLHGHMQVHERNKDGSQSGSRMEDWKMKDTQKCSQCEEGFDFPEDLQKHIAECHPECSPNEDRAA
LQCMYCHELFVEETSLMNHIEQVHGGEKKNSCSICSESFLTVEELYSHMDSHQQPESCNHSNSPSLVTVG
YTSVSSTTPDSNLSVDSSTMVEAAPPIPKSRGRKRAAQQTSDMTGPSSKQAKVTYSCIYCNKQLFSSLAV
LQIHLKTMHLDKPEQAHICQYCLEVLPSLYNLNEHLKQVHEAQDPGLIVSAMPAIVYQCNFCSEVVNDLN
TLQEHIRCSHGFANPAAKDSNAFFCPHCYMGFLTDSSLEEHIRQVHCDLSGSRFGSPVLGTPKEPVVEVY
SCSYCTNSPIFNSVLKLNKHIKENHKNIPLALNYIHNGKKSRALSPLSPVAIEQTTLKMMQTVGGGPARA
SGEYICNQCGAKYTSLDSFQTHLKTHLDTVLPKLTCPQCNKEFPNQESLLKHVTIHFMITSTYYICESCD
KQFTSVDDLQKHLLDMHTFVFFRCTLCQEVFDSKVSIQLHLAVKHSNEKKVYRCTSCNWDFRNETDLQLH
VKHNHLENQGKVHKCIFCGESFGTEVELQCHITTHSKKYNCRFCSKAFHAVILLEKHLREKHCVFETKTP
NCGTNGASEQVQKEEAELQTLLTNSQESHNSHDGSEEDVDSSEPMYGCDICGAAYTMETLLQNHQLRDHN
IRPGESAIVKKKAELIKGNYKCNVCSRTFFSENGLREHMQTHLGPVKHYMCPICGERFPSLLTLTEHKVT
HSKSLDTGNCRICKMPLQSEEEFLEHCQMHPDLRNSLTGFRCVVCMQTVTSTLELKIHGTFHMQKTGNGS
SVQTTGRGQHVQKLYKCASCLKEFRSKQDLVKLDINGLPYGLCAGCVNLSKSSSPGLSLPPGASRPGLGQ
NESLSAMEGKGKAGGLKTRCSSCNVKFESESELQNHIQTVHRELVPDANSTQLKTPQVSPMPRISPSQSD
EKKTYQCIKCQMVFYNEWDIQVHVANHMIDEGLNHECKLCSQTFDSPAKLQCHLIEHSFEGMGGTFKCPV
CFTVFVQANKLQQHIFSAHGQEDKIYDCTQCPQKFFFQTELQNHTMTQHSS

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC021376
ORF Size 3935 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC021376, AAH21376
RefSeq Size 4781 bp
RefSeq ORF 3935 bp
Locus ID 225207
Gene Summary Transcription factor that can both act as an activator or a repressor depending on the context. Involved in BMP signaling and in the regulation of the immature compartment of the hematopoietic system. Associates with SMADs in response to BMP2 leading to activate transcription of BMP target genes. Acts as a transcriptional repressor via its interaction with EBF1, a transcription factor involved specification of B-cell lineage; this interaction preventing EBF1 to bind DNA and activate target genes.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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