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Med23 (NM_027347) Mouse Tagged ORF Clone
CAT＃: MG211949
- TrueORF®
Med23 (tGFP-tagged) - Mouse cofactor required for Sp1 transcriptional activation, subunit 3 (Crsp3)
ORF Plasmid: DDK
"NM_027347" in other vectors (4)
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CNY 11,880.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 130kDa; 3000002A17Rik; Crsp3; ESTM7; mKIAA1216; Sur2; X83317 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG211949 representing NM_027347
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTACCGATGGAGACGCAACTGCAGAGCATTTTCGAGGAGGTGGTGAAAACAGAAATTATAGAAGAGG CCTTCCCAGGAATGTTTATGGATACCCCCGAGGATGAAAAAACAAAACTAATTAGCTGCTTGGCGGCCTT CCGGCAGTTTTGGAGTGGACTTTCTCAGGAGTCTCATGAACAGTGTGTTCAGTGGATTGTTAAATTTATT CATGGCCAACACAGCCCCAAAAGAATTTCTTTCCTCTATGACTGCTTAGCAATGGCAGTTGAGACTGGTC TCCTTCCACCCAGGATGGTTTGTGAATCTTTGATAAACTCTGACTCTCTTGAATGGGAAAGAACACAGCT TTGGGCCTTAACATTTAAGCTGGTTCGGAAAATCATTGGAGGAGTGGATTACAAGGGTGTTCGAGACCTT CTCAAAGCGATTTTGGAGAAAATCTTGACAATCCCAAATACAGTGAGCTCTGCTGTTGTCCAGCAGCTTC TGGCAGCAAGAGAGGTTATAGCTTATATCTTAGAACGAAATGCCTGCCTATTGCCGGCCTACTTTGCAGT CACAGAGATCAGAAAACTATATCCTGAGGGAAAACTTCCACACTGGTTACTAGGAAACTTGGTATCAGAC TTTGTGGATACCTTCAGGCCCACAGCAAGGATTAACTCCATTTGTGGCCGATGCAGTCTTCTGCCAGTTG TGAACAACTCGGGAGCCATCTGTAACTCATGGAAACTGGACCCTGCAACTCTGCGATTTCCTTTGAAAGG CCTTTTGCCATATGATAAGGATCTGTTTGAACCGCAGACTGCTTTGTTGAGATATGTACTGGAGCAACCT TATTCCAGGGATATGGTCTGCAATATGCTAGGTTTAAATAAGCAGCACAAGCAGCGCTGCCCTGTGCTGG AGGACCAGTTGGTGGACCTGGTGGTGTACGCCATGGAGAGGTCAGAGACCGAGGAGAAGTTTGACGATGG GGGAACCAGCCAGCTCCTGTGGCAGCACCTTTCCAGCCAGCTCATTTTCTTTGTGCTTTTCCAGTTTGCC AGTTTCCCACATATGGTCCTCTCTCTCCACCAGAAGTTAGCAGGGCGAGGACTGATTAAAGGGCGAGACC ATCTGATGTGGGTGCTGCTGCAGTTCATTTCTGGAAGTATTCAGAAAAATGCCCTCGCTGACTTCCTCCC TGTCATGAAGCTCTTTGACCTGCTGTACCCGGAGAAGGAGTGTATCCCAGTTCCTGATATTAACAAACCC CAGTCAACGCACGCCTTTGCAATGACTTGTATTTGGATTCATCTTAATAGAAAAGCTCAAAATGGCGACT CCACGCTCCAGATCCCAATACCCCATTCCCTGAAGCTGCACCATGAGTTCCTCCAGCAGAGTCTGAGAAA TAAAAGCTTACAGATGAATGACTATAAGATTGCCCTGCTATGTAACGCGTATTCCACAAACTCAGAGTGT TTTACGTTACCTATGGGAGCTCTGGTAGAAACTATTTATGGAAATGGAATTATGAGAGTGCCTCTCCCTG GAACGAGCTGTTTGGCCTCAGCATCAGTGACTCCCTTACCGATGAACCTCCTGGATTCACTGACGGTGCA TGCCAAGATGAGCCTTATTCACAGCATTGCAACCAGAGTGATAAAACTGGCTCATACGAAATCCAGTGTT GCGCTGGCTCCAGCCCTAGTGGAAACTTACAGCCGTTTACTGGTCTACATGGAAATTGAGTCTTTGGGCA TCAAAGGATTTATCAGTCAGCTCCTGCCCACTGTCTTCAAGTCCCACGCCTGGGGCATCCTGCACACACT GCTGGAGATGTTCAGCCACCGAATGCACCACATTCAGCCCCACTACCGAGTTCAGCTCCTGAGCCATCTC CACACACTGGCTGCAGTCGCACAGACCAACCAGAACCAGCTCCATCTGTGTGTGGAGAGCACTGCACTGA GGCTCATCACAGCCCTGGGAAGCTCAGAGGTCCAGCCGCAGTTCACGCGTTTCCTCAATGATCCCAAAAC AGTGCTGTCCGCCGAATCTGAAGAGCTGAACCGAGCCTTGATCCTGACCTTAGCCAGAGCAACCCACGTC ACAGATTTTTTTACAGGATCTGATTCAATACAGGGAACTTGGTGTAAAGATATCCTTCAGACCATCATGA ACTTTACTCCTCATAACTGGGCTTCACATACCCTTAGCTGTTTTCCAGCCCCTCTGCAGGCTTTCTTCAA GCAAAATAACGTGCCCCAGGAAAGCCGTTTTAATCTGAAGAAGAATGTGGAAGAAGAGTACAGGAAGTGG AAGTCAATGACTGATGAAAACGAGATCATCACCCAGTTCTCTGTGCAGGGCTTCCCTCCACTCTTCCTCT GTCTCCTCTGGAAGATGCTCCTGGAGACGGATCATATTAGTCAGATCGGCTACAAGGTCCTGGAGAGGAT TGGGGCCAGGGCCCTGGTGGCCCATGTGAGAACATTTGCAGATTTCCTGGTGTATGAGTTCTCTACGTCA GCAGGAGGTCAGCAACTCAATAAGTGCATTGAAATTCTCAACGACATGGTGTGGAAGTACAACATTGTCA CGCTGGACAGACTCATTCTCTGCCTGGCTATGCGTAGTCATGAAGGAAATGAAGCCCAGGTTTGTTATTT CATAATTCAGTTGCTGTTGCTCAAACCAAATGACTTCAGAAACCGAGTAAGTGACTTTGTGAAGGAGAAT TCCCCAGAGCACTGGCTCCAGAGTGACTGGCACACCAAGCACATGAGCTACCACAAGAAATATCCCGAGA AGCTGTATTTTGAAGGCCTGGCAGAGCAGGTTGACCCTCCTGTACCAATCCAGTCCCCTTATCTGCCCAT CTACTTTGGAAATGTGTGTCTCCGGTTCCTTCCAGTATTTGATATAGTAATCCACAGGTTTTTAGAGTTG CTTCCAGTGTCCAAATCACTAGAGACTCTCCTGGATCACCTAGGGGGCTTATATAAATTCCATGACCGGC CAGTGACTTATCTGTATAATACTTTGCACTATTATGAAATGTGCCTGAGAAACCGTGACCATCTCAAACG AAAACTTGTCCATGCAATCATTGGCTCCCTCAAAGATAACCGGCCACAGGGCTGGTGTCTCAGTGATACC TACCTGAAGCATGCTATGAATGCACGGGAGGACAACCCCTGGGTTCCGGAGGACTCCTACTACTGCAAGC TGATTGGCCGACTGGTAGACACCATGGCGGGCAAGTCTCCAGGACCCTTCCCAAACTGTGACTGGAGATT CAATGAGTTTCCCAACCCCGCTGCCCACGCTCTGCATGTGACGTGTGTGGAACTCATGGCCTTGGCGGTT CCAGGCAAAGATGTGGGGAATGCCCTTCTCAATGTCGTCCTGAAAAGCCAGCCCTTAGTGCCAAGGGAGA ACATTACAGCGTGGATGAATGCAATTGGTCTGATCATCACGGCCCTCCCAGAGCCGTACTGGATTGTGCT TCATGACCGAATTGTGAATGTGATCAGCAGTTCTAGCCTGACGTCAGAGACAGAGTGGGTCGGTTACCCG TTCCGCCTCTTCGACTTCACCGCTTGTCACCAGTCCTACTCGGAGATGAGCTGCAGCTATACGTTAGCTC TAGCCCATGCCGTGTGGCACCACTCCAGCATCGGGCAGCTCTCGCTTATCCCAAAGTTTCTCACTGAGGC GCTTCTCCCGGTGGTGAAGACTGAGTTCCAGTTGCTCTACGTGTACCATCTTGTGGGGCCATTTCTACAG AGGTTCCAACAGGAGAGAACCCGGTGCATGATTGAGATTGGCGTGGCATTTTATGACATGCTGTTGAATG TAGACCAGTGCAGCACGCACTTAAATTACATGGACCCCATCTGCGACTTCCTGTATCATATGAAGTATAT GTTTACTGGTGACAGTGTGAAAGAGCAAGTAGAGAAGATTATCTGTAATTTAAAGCCGGCTTTAAAGCTT CGTCTTCGATTCATCACGCACATTAGCAAGATGGAGCCGGCAGTGCCACCCCAGGCCCTCAACAGCGGGT CTCCAGCACCTCAGTCTAACCAGGTGCCAGCATCTCTGCCGGTGACTCAG AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG211949 representing NM_027347
Red=Cloning site Green=Tags(s) MVPMETQLQSIFEEVVKTEIIEEAFPGMFMDTPEDEKTKLISCLAAFRQFWSGLSQESHEQCVQWIVKFI HGQHSPKRISFLYDCLAMAVETGLLPPRMVCESLINSDSLEWERTQLWALTFKLVRKIIGGVDYKGVRDL LKAILEKILTIPNTVSSAVVQQLLAAREVIAYILERNACLLPAYFAVTEIRKLYPEGKLPHWLLGNLVSD FVDTFRPTARINSICGRCSLLPVVNNSGAICNSWKLDPATLRFPLKGLLPYDKDLFEPQTALLRYVLEQP YSRDMVCNMLGLNKQHKQRCPVLEDQLVDLVVYAMERSETEEKFDDGGTSQLLWQHLSSQLIFFVLFQFA SFPHMVLSLHQKLAGRGLIKGRDHLMWVLLQFISGSIQKNALADFLPVMKLFDLLYPEKECIPVPDINKP QSTHAFAMTCIWIHLNRKAQNGDSTLQIPIPHSLKLHHEFLQQSLRNKSLQMNDYKIALLCNAYSTNSEC FTLPMGALVETIYGNGIMRVPLPGTSCLASASVTPLPMNLLDSLTVHAKMSLIHSIATRVIKLAHTKSSV ALAPALVETYSRLLVYMEIESLGIKGFISQLLPTVFKSHAWGILHTLLEMFSHRMHHIQPHYRVQLLSHL HTLAAVAQTNQNQLHLCVESTALRLITALGSSEVQPQFTRFLNDPKTVLSAESEELNRALILTLARATHV TDFFTGSDSIQGTWCKDILQTIMNFTPHNWASHTLSCFPAPLQAFFKQNNVPQESRFNLKKNVEEEYRKW KSMTDENEIITQFSVQGFPPLFLCLLWKMLLETDHISQIGYKVLERIGARALVAHVRTFADFLVYEFSTS AGGQQLNKCIEILNDMVWKYNIVTLDRLILCLAMRSHEGNEAQVCYFIIQLLLLKPNDFRNRVSDFVKEN SPEHWLQSDWHTKHMSYHKKYPEKLYFEGLAEQVDPPVPIQSPYLPIYFGNVCLRFLPVFDIVIHRFLEL LPVSKSLETLLDHLGGLYKFHDRPVTYLYNTLHYYEMCLRNRDHLKRKLVHAIIGSLKDNRPQGWCLSDT YLKHAMNAREDNPWVPEDSYYCKLIGRLVDTMAGKSPGPFPNCDWRFNEFPNPAAHALHVTCVELMALAV PGKDVGNALLNVVLKSQPLVPRENITAWMNAIGLIITALPEPYWIVLHDRIVNVISSSSLTSETEWVGYP FRLFDFTACHQSYSEMSCSYTLALAHAVWHHSSIGQLSLIPKFLTEALLPVVKTEFQLLYVYHLVGPFLQ RFQQERTRCMIEIGVAFYDMLLNVDQCSTHLNYMDPICDFLYHMKYMFTGDSVKEQVEKIICNLKPALKL RLRFITHISKMEPAVPPQALNSGSPAPQSNQVPASLPVTQ SGPTRTRRLE - GFP Tag - V |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_027347 |
| ORF Size | 4110 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_027347.3, NP_081623.3 |
| RefSeq Size | 4928 bp |
| RefSeq ORF | 4113 bp |
| Locus ID | 70208 |
| UniProt ID | Q80YQ2 |
| Gene Summary | Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional pre-initiation complex with RNA polymerase II and the general transcription factors (By similarity). Also required for transcriptional activation subsequent to the assembly of the pre-initiation complex. Required for transcriptional activation by adenovirus E1A protein. Required for ELK1-dependent transcriptional activation in response to activated Ras signaling.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC224351 | Med23 (untagged) - Mouse mediator complex subunit 23 (Med23), transcript variant 2, (10ug) |
CNY 17,580.00 |
|
| MR211949 | Med23 (Myc-DDK-tagged) - Mouse mediator complex subunit 23 (Med23), transcript variant 2 |
CNY 9,376.00 |
|
| MR211949L3 | Lenti ORF clone of Med23 (Myc-DDK-tagged) - Mouse mediator complex subunit 23 (Med23), transcript variant 2 |
CNY 12,730.00 |
|
| MR211949L4 | Lenti ORF clone of Med23 (mGFP-tagged) - Mouse mediator complex subunit 23 (Med23), transcript variant 2 |
CNY 12,730.00 |
