Prx (BC068135) Mouse Tagged ORF Clone

CAT#: MG211961

  • TrueORF®

Prx (tGFP-tagged) - Mouse periaxin (cDNA clone MGC:92965 IMAGE:30354483)

ORF Plasmid: DDK tGFP



  "BC068135" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 8,650.00


货期*
3周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms L-Periaxin
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG211961 representing BC068135
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGACCCCTTGCTGCCCCTCGCAGGAGCTGAGACGGGCGGAGTTGGTGGAGATTATCGTGGAGACCG
AGGCACAGACCGGGGTCAGCGGCTTCAACGTAGCAGGCGGCGGCAAAGAAGGAATCTTTGTCCGTGAGCT
GCGAGAGGACTCACCGGCAGCTAAGAGCCTCAGCTTGCAAGAAGGGGACCAGCTGCTGAGTGCCCGTGTG
TTCTTTGAGAACTTCAAATATGAGGATGCACTTCGCCTGCTGCAATGCGCAGAGCCCTACAAGGTCTCCT
TCTGCTTGAAGCGCACTGTGCCCACCGGGGATCTGGCACTGAGGCCCGGGACGGTGTCTGGATACGAGAT
GAAGGGCCCACGGGCCAAAGTGGCCAAGCTGAACATCCAGAGTCTGGCCCCTGTGAAGAAGAAGAAGATG
GTGACTGGGGCCCTGGGGACCCCTGCAGATTTGGCCCCTGTTGACGTCGAGTTCTCTTTTCCCAAGTTCT
CCCGACTGCGTCGGGGTCTCAAAGCCGAGGCTGTCAAGGGACCTGTCCCAGCTGCCCCAGCCCGTCGCCG
CCTCCAGCTGCCTCGGCTGCGTGTCCGAGAAGTAGCTGAAGAGGCCCAGGTAGCCCGAATGGCTGCTGCT
GCTCCTCCCCCAAGGAAGGCCAAGGCAGAAGCTGAGGCAGCCACAGGAGCTGGGTTCACAGCCCCTCAGT
TAGAGCTAGTTGGGCCTCGGCTGCCTAGTGCCGAGGTGGGTGTCCCTCAGGTCTCAGTTCCCAAGGGGAC
CCCATCAACAGAGGCAGCCAGCGGCTTTGCCCTTCACCTGCCAACCCTTGGGCTAGGTGCCCCAGCTGCA
CCGGCTGTGGAGCCCCCAGCCACGGGAATCCAGGTTCCACAAGTGGAACTCCCCACCCTGCCCTCTCTAC
CCACTCTTCCCACACTTCCATGCCTGGACACCCAGGAAGGAGCTGCAGTGGTAAAAGTCCCTACCCTGGA
TGTGGCAGCTCCGTCTATGGGGGTGGACCTGGCTTTGCCGGGTGCAGAGGTGGAGGCCCAGGGAGAGGTT
CCTGAAGTGGCCCTCAAGATGCCCCGGCTCAGTTTCCCCCGTTTTGGGATTCGGGGGAAGGAAGCCACTG
AAGCCAAAGTAGTCAAGGGCAGCCCTGAGGCCAAAGCAAAGGGTCCCAGACTTCGAATGCCCACCTTTGG
GCTTTCTCTCCTGGAACCCCGGCCCTCTGGCCCTGAAGCTGTTGCTGAGAGCAAGCTGAAGCTACCCACC
CTCAAGATGCCCTCTTTCGGCATTGGTGTGACTGGGCCTGAGGTCAAGGCACCCAAGGGGCCCGAAGTAA
AGCTCCCTAAGGTTCCTGAGGTCAAACTCCCGAAAGTGCCCGAGGCAGCCATTCCAGATGTGCAACTCCC
TGAGGTACAGCTGCCCAAAATGTCAGACATGAAACTTCCAAAGATCCCTGAGATGGTTGTACCCGACGTT
CGTCTTCCGGAAGTGCAGCTGCCCAAAGTCCCTGAGATGAAAGTCCCAGAGATGAAGCTCCCGAAGGTGC
CCGAGATGGCCGTGCCCGATGTACACCTTCCAGATGTACAGCTCCCGAAAGTCCCAGAGATGAAGCTCCC
GAAGGTGCCCGAGATGGCCGTGCCCGATGTACACCTTCCAGATGTACAGCTCCCGAAAGTTCCAGAGATG
AAGCTACCAGAGATGAAGCTCCCGAAGGTGCCGGAGATGGCCGTGCCGGATGTACGACTCCCGGAAGTTC
AGCTGCCCAAAGTGTCTGAGGTGAAGCTCCCAAAGATGCCTGAGATGGCCGTGCCTGATGTCCACCTCCC
GGAGCTACAACTTCCCAAAATGTCCGAGGTGAAGCTCCCAAAGATGCCCGAGATGGCCGTGCCCGATGTT
CGCCTCCCGGAAGTTCAGCTGCCCAAAGTGTCAGAGATGAAACTCCCTAAGATGCCAGAGATGACCATGC
CCGACATTCGCCTCCCAGAAGTTCAGTTGCCCAAAGTGCCTGACATTAAACTTCCTGAAATGAAGCTTCC
AGAAATAAAACTCCCCAAAGTGCCTGACATGGCAGTGCCTGATGTCCCCCTTCCAGAGCTGCAGCTGCCC
AAAGTGTCGGACATTCGGCTGCCTGAAATGCAAGTGTCACAGGTCCCAGAGGTGCAGCTTCCCAAGATGC
CAGAGATGAAGTTGTCCAAGGTTCCTGAGGTACAGAGGAAATCTGCAGGGGCGGAGCAGGCAAAAGGGAC
TGAATTTAGTTTCAAGTTGCCCAAGATGACCATGCCCAAGTTGGGGAAAGTGGGCAAGCCTGGGGAGGCA
AGTATTGAGGTTCCAGACAAACTCATGACACTTCCCTGTCTGCAGCCAGAGGTGGGCACTGAGGCATCCC
ATGTTGGTGTCCCTTCCCTCTCTCTCCCCTCTGTGGAGCTTGACTTGCCTGGGGCCCTGGGCCTGGAGGG
ACAAGTCCAAGAAGCTGTCCCAGGCAAAGTGGAGAAGCCAGAGGGCCCCAGGGTAGCAGTGGGTGTTGGA
GAGGTGGGCTTTCGTGTGCCCTCTGTGGAGATTGTCACTCCTCAGCTGCCCACAGTTGAAGTTGAGAAAG
AGCAGCTAGAGATGGTGGAGATGAAAGTCAAACCCTCTTCCAAGTTCTCTCTGCCCAAATTCGGACTTTC
AGGGCCCAAAGCTGTCAAGGGAGAGGTGGAGGGGCCTGGGCGAGCCACCAAGCTGAAGGTTTCCAAGTTT
ACCATCTCACTTCCCAAAGCTCGAGCAGGGACTGAGGCCGAAGCGAAGGGAGCTGGGGAAGCCGGGTTGC
TGCCAGCGCTGGATCTGTCCATCCCACAGCTCAGCCTGGATGCCCAGCTGCCCTCAGGCAAGGTGGAAGT
AGCTGATAGCAAGCCTAAATCGTCCAGATTTGCTCTGCCCAAGTTTGGGGTGAAAGGCCGGGACTCTGAG
GCTGATGTACTGGTGGCAGGGGAGGCTGAGCTTGAGGGAAAGGGTTGGGGCTGGGATGGGAAGGTGAAGA
TGCCCAAGCTGAAAATGCCATCTTTTGGGTTGTCCCGAGGAAAGGAAGCAGAAACTCAGGATGGACGTGT
CAGCCCCGGGGAAAAGCTGGAGGCCATAGCTGGGCAGCTTAAGATCCCTGCGGTGGAATTGGTCACACCG
GGAGCTCAGGAGACAGAGAAGGTCACCAGTGGAGTGAAGCCGTCAGGCCTCCAGGTGTCCACCACTGGGC
AGGTGGTTGCAGAGGGCCAGGAGAGTGTGCAGAGGGTGTCCACACTAGGTATCTCTTTGCCCCAGGTGGA
ATTGGCCAGCTTTGGGGAGGCAGGCCCTGAGATCGTAGCCCCTTCTGCAGAGGGCACAGCAGGCTCTAGG
GTCCAGGTGCCACAGGTGATGCTGGAGCTACCTGGAACCCAGGTGGCAGGGGGTGATCTGTTAGTGGGTG
AGGGCATCTTCAAGATGCCCACAGTGACAGTGCCCCAGCTAGAGCTGGATGTGGGGCTGGGCCATGAAGC
CCAGGCTGGTGAAGCAGCCAAGAGTGAGGGTGGGATAAAGTTGAAGTTGCCCACACTGGGGACCGGAAGC
AGAGGAGAGGGCGTTGAGCCCCAGGGCCCCGAGGCCCAGCGCACCTTCCACCTCTCATTGCCCGATGTGG
AACTCACGTCACCAGTGAGTAGCCACGCTGAGTACCAGGTAGTTGAGGGTGATGGGGATGGTGGGCACAA
ACTCAAGGTTCGGCTGCCCCTGTTTGGTCTGGCAAAGGCCAAGGAAGGGATAGAAGTTGGAGAAAAGGCT
AAGAGTCCAAAGCTCAGGCTACCCCGAGTGGGCTTCAGCCAGAGTGAGTCGGTCTCCGGAGAAGGCTCTC
CAAGTCCTGAGGAGGAGGAAGAAGGCAGTGGGGAAGGGGCTTCCAGTCGCCGGGGTAGGGTAAGGGTCCG
CCTGCCTCGGGTAGGCTTGGCTTCCCCTTCTAAAGTCTCTAAGGGACAGGAGGGTGATGCAACCTCCAAG
TCCCCAGTTGGGGAGAAGTCACCCAAATTCCGTTTTCCTAGGGTGTCCTTAAGCCCCAAGGCCCGGAGTG
GGAGTAGGGACCGGGAAGAAGGTGGATTCAGGGTCCGACTGCCCAGTGTGGGATTTTCAGAAACAGCAGT
TCCAGGTTCCACCAGGATTGAGGGAACCCAGGCTGCTGCCATC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG211961 representing BC068135
Red=Cloning site Green=Tags(s)

MATPCCPSQELRRAELVEIIVETEAQTGVSGFNVAGGGKEGIFVRELREDSPAAKSLSLQEGDQLLSARV
FFENFKYEDALRLLQCAEPYKVSFCLKRTVPTGDLALRPGTVSGYEMKGPRAKVAKLNIQSLAPVKKKKM
VTGALGTPADLAPVDVEFSFPKFSRLRRGLKAEAVKGPVPAAPARRRLQLPRLRVREVAEEAQVARMAAA
APPPRKAKAEAEAATGAGFTAPQLELVGPRLPSAEVGVPQVSVPKGTPSTEAASGFALHLPTLGLGAPAA
PAVEPPATGIQVPQVELPTLPSLPTLPTLPCLDTQEGAAVVKVPTLDVAAPSMGVDLALPGAEVEAQGEV
PEVALKMPRLSFPRFGIRGKEATEAKVVKGSPEAKAKGPRLRMPTFGLSLLEPRPSGPEAVAESKLKLPT
LKMPSFGIGVTGPEVKAPKGPEVKLPKVPEVKLPKVPEAAIPDVQLPEVQLPKMSDMKLPKIPEMVVPDV
RLPEVQLPKVPEMKVPEMKLPKVPEMAVPDVHLPDVQLPKVPEMKLPKVPEMAVPDVHLPDVQLPKVPEM
KLPEMKLPKVPEMAVPDVRLPEVQLPKVSEVKLPKMPEMAVPDVHLPELQLPKMSEVKLPKMPEMAVPDV
RLPEVQLPKVSEMKLPKMPEMTMPDIRLPEVQLPKVPDIKLPEMKLPEIKLPKVPDMAVPDVPLPELQLP
KVSDIRLPEMQVSQVPEVQLPKMPEMKLSKVPEVQRKSAGAEQAKGTEFSFKLPKMTMPKLGKVGKPGEA
SIEVPDKLMTLPCLQPEVGTEASHVGVPSLSLPSVELDLPGALGLEGQVQEAVPGKVEKPEGPRVAVGVG
EVGFRVPSVEIVTPQLPTVEVEKEQLEMVEMKVKPSSKFSLPKFGLSGPKAVKGEVEGPGRATKLKVSKF
TISLPKARAGTEAEAKGAGEAGLLPALDLSIPQLSLDAQLPSGKVEVADSKPKSSRFALPKFGVKGRDSE
ADVLVAGEAELEGKGWGWDGKVKMPKLKMPSFGLSRGKEAETQDGRVSPGEKLEAIAGQLKIPAVELVTP
GAQETEKVTSGVKPSGLQVSTTGQVVAEGQESVQRVSTLGISLPQVELASFGEAGPEIVAPSAEGTAGSR
VQVPQVMLELPGTQVAGGDLLVGEGIFKMPTVTVPQLELDVGLGHEAQAGEAAKSEGGIKLKLPTLGTGS
RGEGVEPQGPEAQRTFHLSLPDVELTSPVSSHAEYQVVEGDGDGGHKLKVRLPLFGLAKAKEGIEVGEKA
KSPKLRLPRVGFSQSESVSGEGSPSPEEEEEGSGEGASSRRGRVRVRLPRVGLASPSKVSKGQEGDATSK
SPVGEKSPKFRFPRVSLSPKARSGSRDREEGGFRVRLPSVGFSETAVPGSTRIEGTQAAAI

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC068135
ORF Size 4173 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC068135.1
RefSeq Size 4502 bp
RefSeq ORF 4175 bp
Locus ID 19153
Gene Summary Scaffolding protein that functions as part of a dystroglycan complex in Schwann cells, and as part of EZR and AHNAK-containing complexes in eye lens fiber cells (PubMed:11430802, PubMed:21745462, PubMed:22764250). Required for the maintenance of the peripheral myelin sheath that is essential for normal transmission of nerve impulses and normal perception of sensory stimuli (PubMed:10839370). Required for normal transport of MBP mRNA from the perinuclear to the paranodal regions (PubMed:15356632). Required for normal remyelination after nerve injury (PubMed:10839370). Required for normal elongation of Schwann cells and normal length of the internodes between the nodes of Ranvier. The demyelinated nodes of Ranvier permit saltatory transmission of nerve impulses; shorter internodes cause slower transmission of nerve impulses (PubMed:15356632, PubMed:23022068). Required for the formation of appositions between the abaxonal surface of the myelin sheath and the Schwann cell plasma membrane; the Schwann cell cytoplasm is restricted to regions between these appositions (PubMed:15356632, PubMed:23022068). Required for the formation of Cajal bands and of Schmidt-Lanterman incisures that correspond to short, cytoplasm-filled regions on myelinated nerves (PubMed:23022068, PubMed:22764250). Recruits DRP2 to the Schwann cell plasma membrane (PubMed:11430802, PubMed:23022068, PubMed:22764250). Required for normal protein composition of the eye lens fiber cell plasma membrane and normal eye lens fiber cell morphology (PubMed:21745462).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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