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Col2a1 (BC052326) Mouse Tagged ORF Clone
CAT＃: MG211978
- TrueORF®
Col2a1 (tGFP-tagged) - Mouse procollagen, type II, alpha 1 (cDNA clone MGC:58872 IMAGE:6315379)
ORF Plasmid: DDK
"BC052326" in other vectors (4)
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CNY 15,580.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Col2; Col2a; Col2a-1; Del1; Dmm; Lpk; Rgsc856 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG211978 representing BC052326
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATCCGCCTCGGGGCTCCCCAGTCGCTGGTGCTGCTGACGCTGCTCATCGCCGCGGTCCTACGGTGTC AGGGCCAGGATGCCCGAAAATTAGGGCCAAAGGGGCAGAAAGGAGAACCTGGAGATATCAGAGATATCAT AGGACCCAGAGGACCTCCTGGCCCTCAGGGACCTGCAGGTGAACAAGGACCCAGAGGTGATCGTGGTGAC AAGGGAGAAAAGGGTGCGCCTGGACCCCGTGGCAGAGATGGAGAACCTGGTACCCCTGGAAATCCTGGCC CCGCTGGCCCTCCAGGTCCCCCTGGTCCCCCTGGCCTTAGTGCAGGAAACTTCGCGGCTCAGATGGCTGG AGGGTATGACGAGAAGGCTGGTGGTGCCCAGATGGGAGTCATGCAAGGGCCCATGGGCCCCATGGGACCC CGTGGACCCCCAGGCCCTGCCGGTGCCCCCGGCCCTCAAGGATTTCAAGGCAATCCTGGTGAACCTGGCG AGCCTGGTGTCTCTGGTCCCATGGGTCCCCGAGGTCCTCCTGGCCCTGCTGGAAAACCTGGTGACGACGG TGAAGCTGGGAAGCCCGGAAAGTCTGGGGAAAGAGGCCTCCCTGGCCCTCAGGGTGCTCGTGGATTCCCA GGAACCCCGGGTCTCCCCGGTGTCAAGGGTCACAGAGGTTACCCAGGCCTCGACGGTGCTAAGGGGGAAG CTGGTGCTCCGGGTGTGAAGGGTGAGAGTGGTTCCCCTGGTGAGAACGGATCCCCGGGCCCAATGGGTCC CCGTGGCCTGCCTGGTGAGAGAGGACGGACTGGCCCTGCTGGTGCTGCTGGTGCTCGGGGTAACGATGGC CAGCCAGGCCCCGCTGGACCTCCGGGTCCTGTGGGTCCCGCAGGTGGTCCTGGCTTCCCTGGTGCTCCTG GTGCCAAGGGCGAAGCTGGTCCCACTGGTGCTCGCGGTCCTGAAGGTGCTCAAGGTTCTCGTGGCGAGCC TGGCAATCCTGGGTCCCCTGGGCCTGCAGGTGCTTCTGGTAACCCAGGGACTGATGGTATTCCTGGAGCC AAAGGATCCGCTGGTGCTCCTGGAATTGCTGGTGCCCCTGGCTTCCCTGGGCCCCGTGGCCCTCCCGGTC CTCAAGGTGCAACTGGTCCCCTTGGCCCCAAAGGTCAGGCGGGTGAACCTGGCATTGCTGGCTTTAAAGG TGATCAAGGCCCCAAGGGAGAGACTGGACCTGCTGGGCCCCAAGGAGCCCCTGGCCCCGCTGGTGAAGAA GGCAAACGAGGTGCTCGAGGAGAGCCGGGTGGTGCTGGACCAATCGGACCCCCTGGAGAGAGAGGTGCTC CTGGCAACCGTGGATTCCCAGGTCAAGATGGTCTGGCAGGTCCCAAGGGTGCCCCTGGAGAGCGAGGGCC CAGTGGCTTGACTGGTCCCAAGGGAGCCAACGGTGACCCGGGTCGTCCTGGAGAACCTGGTCTTCCTGGA GCCAGGGGTCTTACCGGTCGCCCTGGTGACGCTGGTCCTCAAGGCAAAGTTGGTCCTTCTGGAGCCCCTG GTGAAGACGGTCGCCCTGGACCTCCTGGTCCTCAGGGAGCTCGTGGGCAGCCTGGCGTCATGGGTTTCCC TGGCCCCAAAGGTGCCAACGGCGAGCCTGGCAAAGCTGGTGAGAAGGGTCTGGCTGGCGCTCCTGGTCTG AGAGGTCTTCCTGGAAAAGACGGTGAGACGGGAGCCGCAGGACCCCCCGGCCCCAGTGGACCTGCTGGTG AACGAGGCGAGCAGGGCGCTCCTGGACCATCAGGGTTCCAGGGACTTCCTGGCCCTCCCGGTCCCCCAGG TGAAGGTGGAAAGCAAGGTGACCAGGGTATTCCTGGTGAAGCTGGAGCTCCTGGCCTTGTGGGTCCTCGG GGCGAGCGAGGTTTCCCAGGTGAACGTGGCTCTCCTGGTGCTCAGGGCCTTCAGGGTCCCCGAGGCCTCC CTGGCACTCCTGGTACTGATGGTCCCAAAGGTGCAGCTGGCCCAGATGGCCCCCCTGGGGCTCAGGGGCC TCCAGGTCTACAGGGAATGCCTGGTGAGAGAGGAGCCGCTGGCATTGCTGGGCCCAAGGGAGACAGAGGC GATGTTGGCGAGAAAGGCCCAGAGGGAGCTCCTGGGAAGGATGGCGGCCGAGGTCTGACTGGGCCCATCG GACCCCCAGGCCCAGCAGGGGCCAACGGCGAGAAGGGAGAAGTCGGACCTCCTGGCCCGTCAGGAAGTAC CGGAGCTCGAGGTGCCCCGGGTGAACGCGGAGAGACCGGGCCACCTGGACCTGCTGGATTCGCTGGCCCT CCTGGTGCTGATGGCCAGCCTGGTGCCAAGGGTGATCAAGGAGAAGCCGGACAGAAAGGAGATGCTGGTG CCCCCGGCCCACAAGGCCCCTCGGGAGCCCCTGGGCCACAGGGTCCTACTGGAGTGACTGGTCCTAAGGG AGCCCGAGGTGCCCAAGGTCCCCCGGGAGCCACCGGATTCCCTGGAGCTGCTGGCCGAGTTGGACCCCCA GGTGCTAATGGCAATCCTGGACCCGCCGGTCCCCCTGGTCCTGCTGGAAAAGATGGTCCCAAAGGTGTTC GAGGAGACAGTGGCCCCCCTGGCAGAGCTGGTGACCCCGGTCTTCAAGGTCCTGCAGGAGCTCCTGGCGA GAAAGGAGAACCTGGAGATGATGGTCCCTCTGGTCTTGATGGTCCTCCAGGTCCCCAGGGGCTGGCTGGT CAAAGGGGCATTGTTGGTCTGCCTGGTCAGCGTGGTGAGAGAGGATTCCCCGGCCTTCCCGGCCCATCGG GTGAGCCCGGCAAGCAGGGTGCACCTGGCGCGTCTGGAGACAGAGGTCCTCCTGGTCCTGTGGGGCCTCC TGGCCTGACAGGGCCTGCAGGTGAACCTGGACGAGAGGGCAGCCCTGGTGCTGATGGACCCCCTGGAAGA GATGGTGCAGCTGGAGTCAAGGGAGATCGTGGTGAGACTGGAGCACTGGGTGCCCCTGGAGCTCCTGGGC CCCCAGGCTCTCCTGGTCCTGCTGGCCCAACTGGCAAACAAGGAGACAGAGGAGAGGCTGGTGCACAAGG TCCTATGGGTCCCTCAGGACCTGCTGGAGCCCGTGGGATTGCAGGCCCTCAAGGCCCCCGAGGTGACAAA GGAGAATCTGGAGAGCAGGGCGAGAGGGGACTGAAGGGACACCGAGGTTTCACTGGACTGCAGGGTCTGC CTGGCCCTCCGGGTCCTTCTGGAGATCAGGGTGCTTCTGGCCCTGCTGGTCCTTCTGGCCCTAGAGGTCC ACCTGGCCCTGTTGGTCCCTCTGGCAAAGATGGCTCTAATGGAATCCCTGGCCCCATCGGGCCTCCAGGT CCCCGTGGACGCTCAGGAGAAACAGGCCCTGTTGGTCCCCCTGGAAGTCCCGGTCCTCCTGGCCCTCCAG GTCCTCCTGGTCCTGGCATCGACATGTCAGCCTTTGCTGGCTTAGGGCAGAGAGAGAAGGGCCCCGACCC CATGCAGTACATGCGGGCCGACGAGGCAGACAGTACCTTGAGACAGCACGACGTGGAGGTGGACGCTACA CTCAAGTCACTGAACAACCAGATTGAGAGCATCCGCAGCCCCGACGGCTCCCGCAAGAACCCTGCTCGCA CTTGCCAAGACCTGAAACTCTGCCACCCCGAGTGGAAGAGCGGAGACTACTGGATTGATCCCAACCAGGG CTGCACCTTGGACGCCATGAAAGTTTTCTGCAACATGGAGACCGGCGAGACTTGCGTCTACCCCAACCCA GCGACTGTCCCTCGGAAAAACTGGTGGAGCAGCAAGAGCAAGGAAAAGAAACACATCTGGTTTGGAGAGA CCATGAACGGTGGCTTCCACTTCAGCTATGGCGATGGCAACCTGGCTCCCAACACCGCTAACGTCCAGAT GACTTTCCTCCGTCTACTGTCCACTGAGGGCTCCCAGAACATCACCTACCACTGTAAGAACAGCATCGCC TACCTGGACGAAGCGGCTGGCAACCTCAAGAAGGCCTTGCTCATCCAGGGCTCCAATGATGTAGAGATGA GGGCCGAGGGCAACAGCAGGTTCACATACACTGCCCTGAAGGATGGCTGCACGAAACACACTGGTAAGTG GGGCAAGACCGTCATCGAGTACCGATCACAGAAGACCTCCCGCCTTCCCATTATTGACATCGCACCCATG GACATTGGAGGGGCTGAACAGGAATTTGGTGTGGACATAGGGCCTGTCTGCTTCTTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG211978 representing BC052326
Red=Cloning site Green=Tags(s) MIRLGAPQSLVLLTLLIAAVLRCQGQDARKLGPKGQKGEPGDIRDIIGPRGPPGPQGPAGEQGPRGDRGD KGEKGAPGPRGRDGEPGTPGNPGPAGPPGPPGPPGLSAGNFAAQMAGGYDEKAGGAQMGVMQGPMGPMGP RGPPGPAGAPGPQGFQGNPGEPGEPGVSGPMGPRGPPGPAGKPGDDGEAGKPGKSGERGLPGPQGARGFP GTPGLPGVKGHRGYPGLDGAKGEAGAPGVKGESGSPGENGSPGPMGPRGLPGERGRTGPAGAAGARGNDG QPGPAGPPGPVGPAGGPGFPGAPGAKGEAGPTGARGPEGAQGSRGEPGNPGSPGPAGASGNPGTDGIPGA KGSAGAPGIAGAPGFPGPRGPPGPQGATGPLGPKGQAGEPGIAGFKGDQGPKGETGPAGPQGAPGPAGEE GKRGARGEPGGAGPIGPPGERGAPGNRGFPGQDGLAGPKGAPGERGPSGLTGPKGANGDPGRPGEPGLPG ARGLTGRPGDAGPQGKVGPSGAPGEDGRPGPPGPQGARGQPGVMGFPGPKGANGEPGKAGEKGLAGAPGL RGLPGKDGETGAAGPPGPSGPAGERGEQGAPGPSGFQGLPGPPGPPGEGGKQGDQGIPGEAGAPGLVGPR GERGFPGERGSPGAQGLQGPRGLPGTPGTDGPKGAAGPDGPPGAQGPPGLQGMPGERGAAGIAGPKGDRG DVGEKGPEGAPGKDGGRGLTGPIGPPGPAGANGEKGEVGPPGPSGSTGARGAPGERGETGPPGPAGFAGP PGADGQPGAKGDQGEAGQKGDAGAPGPQGPSGAPGPQGPTGVTGPKGARGAQGPPGATGFPGAAGRVGPP GANGNPGPAGPPGPAGKDGPKGVRGDSGPPGRAGDPGLQGPAGAPGEKGEPGDDGPSGLDGPPGPQGLAG QRGIVGLPGQRGERGFPGLPGPSGEPGKQGAPGASGDRGPPGPVGPPGLTGPAGEPGREGSPGADGPPGR DGAAGVKGDRGETGALGAPGAPGPPGSPGPAGPTGKQGDRGEAGAQGPMGPSGPAGARGIAGPQGPRGDK GESGEQGERGLKGHRGFTGLQGLPGPPGPSGDQGASGPAGPSGPRGPPGPVGPSGKDGSNGIPGPIGPPG PRGRSGETGPVGPPGSPGPPGPPGPPGPGIDMSAFAGLGQREKGPDPMQYMRADEADSTLRQHDVEVDAT LKSLNNQIESIRSPDGSRKNPARTCQDLKLCHPEWKSGDYWIDPNQGCTLDAMKVFCNMETGETCVYPNP ATVPRKNWWSSKSKEKKHIWFGETMNGGFHFSYGDGNLAPNTANVQMTFLRLLSTEGSQNITYHCKNSIA YLDEAAGNLKKALLIQGSNDVEMRAEGNSRFTYTALKDGCTKHTGKWGKTVIEYRSQKTSRLPIIDIAPM DIGGAEQEFGVDIGPVCFL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC052326 |
| ORF Size | 4257 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC052326.1 |
| RefSeq Size | 4856 bp |
| RefSeq ORF | 4259 bp |
| Locus ID | 12824 |
| Gene Summary | This gene encodes the alpha-1 subunit of the fibril-forming type II collagen, the major component of cartilage and the vitreous humor of the eye. The encoded preproprotein forms homotrimeric, triple helical procollagen that undergoes proteolytic processing during fibirl formation. Mice harboring certain mutations in this gene exhibit severe chondrodysplasia characterized by short limbs and trunch, craniofacial deformities and cleft palate. A complete lack of the encoded protein in mice results in postnatal lethality. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar proteolytic processing. [provided by RefSeq, Dec 2015] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC224565 | Col2a1 (untagged) - Mouse procollagen, type II, alpha 1 (cDNA clone MGC:58872 IMAGE:6315379), (10ug) |
CNY 19,190.00 |
|
| MR211978 | Col2a1 (Myc-DDK-tagged) - Mouse procollagen, type II, alpha 1 (cDNA clone MGC:58872 IMAGE:6315379) |
CNY 14,160.00 |
|
| MR211978L3 | Lenti ORF clone of Col2a1 (Myc-DDK-tagged) - Mouse procollagen, type II, alpha 1 (cDNA clone MGC:58872 IMAGE:6315379) |
CNY 16,060.00 |
|
| MR211978L4 | Lenti ORF clone of Col2a1 (mGFP-tagged) - Mouse procollagen, type II, alpha 1 (cDNA clone MGC:58872 IMAGE:6315379) |
CNY 16,060.00 |
