Cpsf1 (BC056388) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG211989 representing BC056388 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTACGCGGTGTACAAGCAGGCGCACCCGCCCACCGGGCTGGAGTTTACCATGTACTGCAACTTCTTCA ACAACAGTGAGCGCAACCTCGTGGTGGCCGGCACCTCGCAGCTCTATGTGTACCGCCTGAACCGCGACGC CGAGGCTCTGACCAAGAATGATGGGAGCACAGAGGGGAAGGCCCACCGAGAGAAACTAGAGCTGGTAGCC TCCTTCTCTTTTTTTGGCAATGTCATGTCCATGGCCAGTGTGCAGCTTGCAGGCGCCAAACGTGATGCCT TGCTTCTCAGTTTCAAAGACGCTAAGCTGTCTGTGGTGGAGTATGACCCAGGCACTCATGACCTGAAGAC CCTGTCTCTACACTACTTTGAGGAGCCTGAACTTCGGGATGGGTTTGTGCAGAACGTGCATACGCCTCGT GTGCGGGTGGACCCTGACGGGCGCTGTGCAGCAATGCTCATCTATGGCACGAGGCTGGTAGTTCTGCCTT TCCGCAGAGAGAGCCTGGCTGAGGAACATGAGGGGCTCATGGGCGAAGGGCAGAGGTCTAGCTTCTTGCC CAGCTACATCATTGATGTACGGGCTCTGGATGAGAAACTACTCAACATCATTGACCTGCAGTTCCTGCAT GGCTACTATGAGCCCACCCTGCTTATCCTGTTTGAGCCCAACCAGACTTGGCCAGGGCGGGTGGCTGTGA GGCAGGACACGTGCTCCATTGTGGCTATCTCGCTGAACATCACACAGAAAGTCCATCCAGTCATCTGGTC CCTCACCAGCTTGCCTTTTGACTGTACCCAGGCCCTGGCTGTGCCCAAGCCCATAGGTGGGGTAGTGATC TTCGCTGTCAACTCACTGTTGTACCTGAACCAGAGTGTTCCCCCATATGGTGTGGCTCTCAATAGTCTCA CCACAGGCACCACCGCTTTCCCATTGCGTACCCAAGAGGGTGTACGGATCACCCTAGACTGCGCACAGGC GGCCTTCATCTCTTATGACAAGATGGTCATCTCCCTCAAGGGTGGTGAGATTTATGTGCTGACCCTCATC ACTGACGGCATGCGAAGTGTCCGAGCGTTTCACTTTGACAAGGCAGCTGCTAGTGTCCTTACCACCAGTA TGGTCACAATGGAGCCCGGATACCTGTTCCTAGGCTCTCGCCTGGGCAATTCCCTCCTCCTCAAGTACAC GGAGAAGCTGCAGGAGCCCCCAGCCAGCTCCGTCCGTGAGGCTGCTGACAAGGAAGAGCCACCCTCAAAG AAGAAGCGAGTGGAGCCTGCAGTGGGCTGGACAGGAGGCAAGACGGTGCCACAGGACGAGGTGGATGAAA TTGAAGTTTATGGCAGTGAGGCCCAGTCAGGCACACAGCTGGCTACTTACTCTTTTGAGGTATGTGACAG CATGCTCAACATTGGACCCTGTGCCAACGCTGCTGTGGGCGAGCCTGCCTTCCTCTCTGAAGAGTTCCAG AATAGCCCTGAGCCAGACCTGGAGATTGTGGTTTGTTCCGGCTACGGGAAGAATGGGGCCCTGTCAGTGT TGCAGAAGAGTATCCGGCCCCAGGTGGTGACAACCTTTGAACTTCCTGGCTGCTATGATATGTGGACAGT CATTGCTCCTGTGCGGAAAGAGGAGGAAGAAACCCCCAAAGCAGAGAGCACAGAGCAGGAGCCCAGTGCC CCGAAAGCAGAGGAGGATGGGCGAAGACATGGCTTCCTTATTCTTAGCCGGGAAGACTCAACCATGATCC TACAGACTGGCCAGGAGATCATGGAGCTGGACACCAGTGGTTTTGCCACTCAGGGTCCTACTGTCTTTGC TGGAAACATTGGGGACAATCGTTACATTGTTCAAGTCTCGCCACTGGGCATTCGCCTGTTGGAAGGCGTG AATCAATTGCACTTCATCCCTGTGGACCTGGGCGCCCCCATCGTGCAGTGTGCCGTGGCTGATCCCTATG TGGTTATCATGAGTGCTGAGGGACACGTTACCATGTTCCTACTCAAGAGCGATTCCTATGGTGGCCGTCA TCACCGCCTGGCACTACACAAGCCTCCACTGCATCATCAGTCCAAGGTGATTGCTCTCTGCCTGTACCGC GATGTCAGTGGCATGTTCACCACTGAGAGCCGCCTGGGTGGGGCCCGTGATGAACTGGGTGGCCGAAGTG GCTCCGAGGCAGAAGGCCTGGGCTCAGAAACGAGCCCCACAGTGGATGATGAGGAAGAGATGCTCTATGG GGACTCCAGTGCCCTCTTCAGTCCCAGCAAAGAGGAGGCCCGTAGGAGCAGCCAGCCACCGGCTGATCGT GACCCTGCACCCTTCAAGGCAGACCCCACTCACTGGTGCCTGCTGGTGCGGGAGAATGGCACCATGGAGA TCTACCAGCTCCCAGACTGGCGGCTGGTATTCCTAGTTAAGAACTTCCCCGTGGGGCAGCGGGTTCTGGT GGACAGTTCATTTGGACAGCCCACTACACAAGGTGAAGTCCGAAAGGAAGAAGCCACACGCCAGGGGGAG CTGCCTTTGGTTAAGGAGGTGTTGTTGGTTGCTCTGGGAAGCCGGCAGAGCAGGCCCTACCTGCTGGTAC ACGTGGACCAGGAGTTGCTTATCTATGAAGCCTTTCCCCATGACTCTCAGCTTGGCCAGGGAAATCTTAA AGTCCGTTTCAAAAAGGTCCCTCACAATATCAACTTCCGTGAAAAGAAGCCAAAGCCGTCCAAGAAGAAG GCCGAAGGTTGCAGCACTGAGGAGGGATCTGGAGGCCGGGGCCGTGTGGCACGTTTCCGCTACTTTGAGG ATATTTATGGCTATTCGGGGGTTTTCATCTGTGGACCCTCACCTCACTGGCTCCTGGTGACTGGCCGCGG AGCTCTGCGTCTGCACCCCATGGGCATTGATGGCCCCATTGACTCCTTTGCTCCTTTCCACAATGTGAAC TGCCCCCGTGGTTTCCTGTACTTCAACAGACAGGGGGAGCTGAGGATCAGCGTCCTGCCTGCCTACCTGT CCTACGATGCCCCATGGCCTGTCAGGAAGATCCCACTCCGATGCACAGCCCACTATGTGGCCTACCATGT GGAATCCAAGGTCTATGCTGTGGCCACTAGTACCAACACACCTTGCACTAGGATCCCACGCATGACTGGC GAGGAAAAAGAATTCGAGGCCATTGAGAGAGACGACAGATACATCCACCCCCAGCAAGAAGCCTTCTCCA TCCAGCTCATCTCCCCGGTCAGCTGGGAGGCCATTCCCAATGCCAGGATCGAGCTAGAGGAATGGGAGCA TGTCACGTGCATGAAGACAGTGTCACTGCGCAGTGAGGAGACCGTGTCAGGCCTAAAGGGCTATGTGGCT GCTGGGACCTGCCTCATGCAGGGGGAGGAGGTCACGTGCCGTGGGCGGATCCTGATCATGGATGTGATTG AAGTGGTGCCTGAGCCTGGGCAGCCCCTGACCAAGAACAAATTCAAGGTCCTGTATGAGAAGGAGCAGAA AGGGCCCGTGACTGCGCTGTGCCACTGCAATGGCCACCTGGTGTCGGCCATTGGCCAAAAAATCTTCCTG TGGAGCCTGCGGGCTAGCGAGCTGACCGGCATGGCCTTCATCGACACCCAGCTGTACATCCACCAGATGA TCAGTGTCAAGAACTTCATCCTGGCAGCAGACGTCATGAAGAGCATCTCACTGCTGCGTTACCAAGAGGA GAGCAAGACGCTGAGCCTGGTGTCCCGGGATGCCAAGCCTTTGGAGGTATACAGCGTGGACTTCATGGTG GACAATGCCCAACTGGGCTTCTTGGTGTCTGACCGAGACCGCAATCTTATGGTGTATATGTATCTGCCAG AAGCCAAAGAGAGTTTTGGGGGCATGCGCCTGCTACGCAGAGCGGATTTCCATGTGGGTGCCCACGTGAA TACGTTCTGGAGGACCCCGTGCCGAGGTGCTGCCGAGGGCCCCAGCAAGAAGTCAGTCGTGTGGGAAAAC AAGCATATCACATGGTTTGCAACCCTTGACGGTGGCATTGGGCTCCTGCTGCCCATGCAAGAAAAGACAT ACCGACGCTTGCTGATGCTGCAGAATGCACTGACGACCATGCTACCCCACCACGCTGGCCTCAACCCCCG CGCCTTCCGAATGTTGCACGTAGACCGGCGCATCTTGCAGAATGCTGTGCGCAACGTCCTTGATGGGGAA CTGCTAAACCGCTACCTATACCTCAGCACCATGGAGCGCAGTGAGCTGGCCAAGAAGATAGGTACCACGC CTGACATTATCCTGGATGACTTGCTGGAGACAGACCGGGTCACGGCTCACTTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG211989 representing BC056388 Red=Cloning site Green=Tags(s) MYAVYKQAHPPTGLEFTMYCNFFNNSERNLVVAGTSQLYVYRLNRDAEALTKNDGSTEGKAHREKLELVA SFSFFGNVMSMASVQLAGAKRDALLLSFKDAKLSVVEYDPGTHDLKTLSLHYFEEPELRDGFVQNVHTPR VRVDPDGRCAAMLIYGTRLVVLPFRRESLAEEHEGLMGEGQRSSFLPSYIIDVRALDEKLLNIIDLQFLH GYYEPTLLILFEPNQTWPGRVAVRQDTCSIVAISLNITQKVHPVIWSLTSLPFDCTQALAVPKPIGGVVI FAVNSLLYLNQSVPPYGVALNSLTTGTTAFPLRTQEGVRITLDCAQAAFISYDKMVISLKGGEIYVLTLI TDGMRSVRAFHFDKAAASVLTTSMVTMEPGYLFLGSRLGNSLLLKYTEKLQEPPASSVREAADKEEPPSK KKRVEPAVGWTGGKTVPQDEVDEIEVYGSEAQSGTQLATYSFEVCDSMLNIGPCANAAVGEPAFLSEEFQ NSPEPDLEIVVCSGYGKNGALSVLQKSIRPQVVTTFELPGCYDMWTVIAPVRKEEEETPKAESTEQEPSA PKAEEDGRRHGFLILSREDSTMILQTGQEIMELDTSGFATQGPTVFAGNIGDNRYIVQVSPLGIRLLEGV NQLHFIPVDLGAPIVQCAVADPYVVIMSAEGHVTMFLLKSDSYGGRHHRLALHKPPLHHQSKVIALCLYR DVSGMFTTESRLGGARDELGGRSGSEAEGLGSETSPTVDDEEEMLYGDSSALFSPSKEEARRSSQPPADR DPAPFKADPTHWCLLVRENGTMEIYQLPDWRLVFLVKNFPVGQRVLVDSSFGQPTTQGEVRKEEATRQGE LPLVKEVLLVALGSRQSRPYLLVHVDQELLIYEAFPHDSQLGQGNLKVRFKKVPHNINFREKKPKPSKKK AEGCSTEEGSGGRGRVARFRYFEDIYGYSGVFICGPSPHWLLVTGRGALRLHPMGIDGPIDSFAPFHNVN CPRGFLYFNRQGELRISVLPAYLSYDAPWPVRKIPLRCTAHYVAYHVESKVYAVATSTNTPCTRIPRMTG EEKEFEAIERDDRYIHPQQEAFSIQLISPVSWEAIPNARIELEEWEHVTCMKTVSLRSEETVSGLKGYVA AGTCLMQGEEVTCRGRILIMDVIEVVPEPGQPLTKNKFKVLYEKEQKGPVTALCHCNGHLVSAIGQKIFL WSLRASELTGMAFIDTQLYIHQMISVKNFILAADVMKSISLLRYQEESKTLSLVSRDAKPLEVYSVDFMV DNAQLGFLVSDRDRNLMVYMYLPEAKESFGGMRLLRRADFHVGAHVNTFWRTPCRGAAEGPSKKSVVWEN KHITWFATLDGGIGLLLPMQEKTYRRLLMLQNALTTMLPHHAGLNPRAFRMLHVDRRILQNAVRNVLDGE LLNRYLYLSTMERSELAKKIGTTPDIILDDLLETDRVTAHF TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC056388
ORF Size	4325 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC056388, AAH56388
RefSeq Size	4538 bp
RefSeq ORF	4325 bp
Locus ID	94230
Gene Summary	Component of the cleavage and polyadenylation specificity factor (CPSF) complex that plays a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. This subunit is involved in the RNA recognition step of the polyadenylation reaction (By similarity).[UniProtKB/Swiss-Prot Function]

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