A2m (BC072642) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	A2mp
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG212004 representing BC072642 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGAAGCGCTGGCTCCCAAGCCTGGCCCTGCTGCCGCTGCCGCCACCGCTGCTGCTGCTGCTGTTGC TGCTGCTGCCCACAAATGCCTCAGCACCACAGAAACCAATCTACATGGTGATGGTTCCCTCCCTGCTCCA CGCTGGCACCCCTGAGAAGGGCTGCCTCCTGTTCAACCACCTAAACGAGACAGTAACAGTGAAAGTATCC ATGGAGTCTGTCCGTGGGAACCAAAGCCTCTTCACTGACCTTGTTGTTGACAAGGACTTATTCCACTGTG CCTCCTTCATCGTCCCACAGTCTTCATCCAATGAGGTGATGTTTCTCACTGTCCAAGTAAAAGGACCAAC TCATGAGTTCAGGAGGAGGAGCACGGTGCTGATCAAGACTAAAGAGAGCCTGGTCTTTGCTCAGACTGAC AAGCCCATCTACAAACCAGGACAGATGGTGAGATTTCGTGTTGTCTCATTGGACGAAAATTTCCACCCCC TTAATGAATTGATTCCTCTACTGTACATTCAGGATTCAAAAAAAAATCGCATTGCACAGTGGCAGAATTT CCGCTTAGAGGGTGGCCTCAAACAGCTGTCCTTCCCCCTCTCCTCAGAGCCCACTCAGGGCTCCTACAAG GTGGTGATACGTACAGAATCAGGCAGGACCGTCGAGCACCCCTTCTCTGTGAAGGAATTTGTGCTTCCCA AGTTTGAAGTCAAAGTTGCAGTACCAGAGACAATCACCATCCTGGAGGAAGAGATGAATGTGTCCGTGTG TGGCATATACACCTATGGGAAGCCTGTTCCAGGACACGTGACTGTGAACATTTGCAGAAAGTACAGTAAT CCTTCTAGCTGCTTTGGTGAAGAATCGCTGGCTTTCTGTGAGAAATTCAGTCAACAGCTAGATGGCCGTG GCTGCTTCTCACAGCTAGTGAAAACCAAGTCTTTCCAGCTAAAGAGGCAAGAGTATGAGATGCAGCTTGA TGTGAATGCCAAGATCCAAGAAGAAGGAACAGGTGTGGAAGAAACCGGTAAAGGGCTCACTAAGATCACA AGAACTATAACAAAACTATCATTTGTGAACGTGGATACACATTTCAGACAAGGAATTCCTTTCGTTGGGC AGGTGCTCCTGGTGGATGGGAGAGGCACCCCTATTCCATATGAAATGATCTTCATTGGAGCTGATGAAGC AAACCAGAACATAAATACTACCACTGATAAGAATGGCCTGGCACGGTTCTCCATCAACACTGATGACATC ATGGGCACGTCCCTAACTGTCAGGGCCAAATACAAAGATAGCAATGTCTGCTATGGATTTAGATGGTTGA CAGAAGAGAACGTAGAGGCTTGGCACACTGCCAACGCTGTCTTCTCACCGAGCAGAAGCTTCGTGCACCT GGAATCCCTGCCCTATAAACTGCGCTGTGAGCAAACCCTGGCGGTCCAGGCTCATTATATTCTAAATGAC GAGGCTGTGCTGGAGCGGAAGGAGTTGGTCTTCTATTATCTGATGATGGCAAAGGGAGGCATTGTTCGGG CAGGGACTCACGTTCTGCCTGTGACGCAGGGACACAAGAAAGGCCATTTCTCCATCCTCATCTCGATGGA GACAGACCTGGCTCCTGTAGCTCGACTGGTCCTTTATACCATTCTGCCCAATGGAGAAGTGGTTGGAGAT ACTGTTAAATATGAGATTGAAAAGTGCCTGGCCAACAAGGTGGATTTGGTCTTCCACCCAAATATCGGTC TTCCGGCCACACGTGCCTTCCTCAGTGTCATGGCTTCTCCTCAGTCTCTCTGTGGCCTGCGAGCTGTGGA CCAAAGCGTACTGCTCACAAAGCCTGAGGCCGAACTCTCTGCATCCCTGGTTTATGATCTGCTACCAGTG AAAGACCTCACTGGCTTCCCTAAGGGTGTGAATCAACAGGAAGAAGACACTAATGGCTGCCTTAAGCAAA ATGACACTTACATTAATGGAATCCTGTACTCCCCAGTACAGAATACAAATGAAGAGGACATGTATGGCTT CCTAAAGGATATGGGCTTAAAGGTATTCACCAACTTGAACATCCGTAAACCCAAAGTCTGTGAACGCCTT GGAGTCAATAAAATACCAGCTGCATACCACCTTGTAAGCCAAGGCCACATGGATGCTTTTCTAGAGTCTT CAGAGTCTCCCACAGAGACTACACGAAGCTACTTCCCTGAGACGTGGATCTGGGACTTGGTGATCGTGGA CTCAACAGGAGTGGCTGAAATGGAAGTGACAGTCCCTGACACCATCACTGAATGGAAGGCTGGGGCCTTC TGCCTGTCCAATGACACTGGGTTGGGCCTCTCTCCTGTGATCGATTTCCAAGCCTTCCAGCCCTTCTTCG TGGATCTCACAATGGCCTACTCCGTGATCCGTGGAGAGGCCTTCACGCTCAAGGCCACTGTGCTGAACTA CCTCCAGACATGCATCCGGGTTGGTGTACAGCTGGAGGCCTCTCCTGATTTCCTAGCTACCCCAGAGGAG AAGGAACAAAAGTCTCACTGCATCTGTATGAACGAGCGGCATACCATGTCCTGGGCGGTGATCCCCAAGT CATTAGGAAATGTGAATTTCACAGTGAGTGCAGAGGCACTGGACTCTAAGGAGCTGTGTAGGAATGAGGT GCCAGTGGTTCCTGAACGTGGAAAGAAAGACACGATCATCAAGTCCCTGCTGGTTGAACCTGAAGGTCTA GAGAATGAAGTGACATTCAACAGTTTGCTTTGTCCAACAGGTGCTGAGGTATCGGAACAGATATCTCTGA AACTGCCATCAGATGTGGTAGAGGAATCTGCCCGAGCTTCTGTCACAGTTTTGGGAGATATATTGGGTTC TGCTATGCAGAACACACAGGATCTCCTTAAGATGCCCTATGGCTGTGGAGAACAGAACATGGTTCTGTTT GCTCCTAATATCTATGTCCTGGACTATCTGAATGAAACAGAGCAGCTGACACAGGAGATCAAGACCAAGG CCATTACCTATCTCAATACGGGTTACCAAAGACAATTAAACTACAAGCACCGGGATGGCTCCTACAGTAC TTTTGGGGATAAACCTGGTAGGAGTCATGCCAATACCTGGCTCACAGCCTTTGTACTGAAGAGTTTTGCT CAGGCTCGAAGATATATCTTCATCGATGAATCACACATCACCCAAGCCCTCACCTGGCTCTCCCAGCAGC AGAAGGACAATGGCTGCTTCAGGAGCTCCGGGTCACTGCTCAACAATGCCATGAAGGGAGGAGTAGAAGA TGAAGTCACTTTGTCTGCCTACATCACCATTGCTCTCCTGGAGATGTCTCTTCCTGTCACTCATCCTGTT GTCCGAAATGCCCTCTTTTGCCTGGACACAGCCTGGAAGTCAGCGAGGAGAGGAGCCAGCGGCAACCATG TCTACACTAAGGCACTGTTGGCCTATGCATTTGCCCTTGCTGGTAACCAGGACACGAAGAAGGAGATACT GAAATCACTCGATGAGGAGGCGGTAAAAGAAGACAATTCTGTCCACTGGACCAGAGCTCAGAAACCCAGA GTGCCAGCAGATCTCTGGTACCAACCCCAGGCTCCCTCGGCTGAGGTAGAGATGACCGCTTATGTGCTCC TGGCTTATCTTACCACTGAGCTGGTTCCAACCCGAGAGGACCTGACTGCTGCAATGCTCATTGTGAAGTG GCTCACAAAGCAGCAGAATTCCCACGGTGGCTTCTCCTCCACCCAGGACACGGTAGTGGCTCTCCATGCT TTGTCCAAATACGGAGCAGCTACTTTCACAAGAGCGAAGAAAGCTGCACATGTGACCATCCAGTCTTCTG GCGCATTTTATACAAAATTCCAAGTGAACAACGACAACCAGTTATTATTACAGAGAGTCACATTGCCAAC TGTGCCTGGGGATTACACCGCAAAGGTAGCTGGAGAAGGGTGTGTCTACCTCCAGACATCCTTGAAATAT AGTGTTCTCCCGAGGGAGAAGGAGTTTCCATTTGCTTTGGTGGTGCAGACTCTGCCTGGGACTTGTGAGG ATCTCAAAGCTCACACCACCTTCCAGATCTCACTCAATATCAGTTACATTGGAAGCCGTTCTGACTCCAA CATGGCAATTGCTGATGTGAAGATGGTGTCTGGCTTCATCCCCTTGAAACCAACAGTGAAAATGCTTGAA AGATCTGTGCATGTGAGCCGGACGGAAGTCAGCAATAACCATGTCTTGATTTACTTGGATAAGGTGTCAA ATCAGATGCTGACCTTGTTCTTCATGGTGCAGCAAGATATTCCAGTGAGAGACCTGAAGCCAGCCATAGT GAAAGTCTATGATTACTACGAGAAAGATGAGTTTGCAGTTGCAAAATACAGTGCTCCCTGCAGTGCAGGT TATGGAAATGCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG212004 representing BC072642 Red=Cloning site Green=Tags(s) MGKRWLPSLALLPLPPPLLLLLLLLLPTNASAPQKPIYMVMVPSLLHAGTPEKGCLLFNHLNETVTVKVS MESVRGNQSLFTDLVVDKDLFHCASFIVPQSSSNEVMFLTVQVKGPTHEFRRRSTVLIKTKESLVFAQTD KPIYKPGQMVRFRVVSLDENFHPLNELIPLLYIQDSKKNRIAQWQNFRLEGGLKQLSFPLSSEPTQGSYK VVIRTESGRTVEHPFSVKEFVLPKFEVKVAVPETITILEEEMNVSVCGIYTYGKPVPGHVTVNICRKYSN PSSCFGEESLAFCEKFSQQLDGRGCFSQLVKTKSFQLKRQEYEMQLDVNAKIQEEGTGVEETGKGLTKIT RTITKLSFVNVDTHFRQGIPFVGQVLLVDGRGTPIPYEMIFIGADEANQNINTTTDKNGLARFSINTDDI MGTSLTVRAKYKDSNVCYGFRWLTEENVEAWHTANAVFSPSRSFVHLESLPYKLRCEQTLAVQAHYILND EAVLERKELVFYYLMMAKGGIVRAGTHVLPVTQGHKKGHFSILISMETDLAPVARLVLYTILPNGEVVGD TVKYEIEKCLANKVDLVFHPNIGLPATRAFLSVMASPQSLCGLRAVDQSVLLTKPEAELSASLVYDLLPV KDLTGFPKGVNQQEEDTNGCLKQNDTYINGILYSPVQNTNEEDMYGFLKDMGLKVFTNLNIRKPKVCERL GVNKIPAAYHLVSQGHMDAFLESSESPTETTRSYFPETWIWDLVIVDSTGVAEMEVTVPDTITEWKAGAF CLSNDTGLGLSPVIDFQAFQPFFVDLTMAYSVIRGEAFTLKATVLNYLQTCIRVGVQLEASPDFLATPEE KEQKSHCICMNERHTMSWAVIPKSLGNVNFTVSAEALDSKELCRNEVPVVPERGKKDTIIKSLLVEPEGL ENEVTFNSLLCPTGAEVSEQISLKLPSDVVEESARASVTVLGDILGSAMQNTQDLLKMPYGCGEQNMVLF APNIYVLDYLNETEQLTQEIKTKAITYLNTGYQRQLNYKHRDGSYSTFGDKPGRSHANTWLTAFVLKSFA QARRYIFIDESHITQALTWLSQQQKDNGCFRSSGSLLNNAMKGGVEDEVTLSAYITIALLEMSLPVTHPV VRNALFCLDTAWKSARRGASGNHVYTKALLAYAFALAGNQDTKKEILKSLDEEAVKEDNSVHWTRAQKPR VPADLWYQPQAPSAEVEMTAYVLLAYLTTELVPTREDLTAAMLIVKWLTKQQNSHGGFSSTQDTVVALHA LSKYGAATFTRAKKAAHVTIQSSGAFYTKFQVNNDNQLLLQRVTLPTVPGDYTAKVAGEGCVYLQTSLKY SVLPREKEFPFALVVQTLPGTCEDLKAHTTFQISLNISYIGSRSDSNMAIADVKMVSGFIPLKPTVKMLE RSVHVSRTEVSNNHVLIYLDKVSNQMLTLFFMVQQDIPVRDLKPAIVKVYDYYEKDEFAVAKYSAPCSAG YGNA TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC072642
ORF Size	4422 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC072642, AAH72642
RefSeq Size	4615 bp
RefSeq ORF	4424 bp
Locus ID	232345
Gene Summary	Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase (By similarity).[UniProtKB/Swiss-Prot Function]

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