Col2a1 (BC082331) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	Col2; Col2a; Col2a-1; Del1; Dmm; Lpk; Rgsc856
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG212008 representing BC082331 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATCCGCCTCGGGGCTCCCCAGTCGCTGGTGCTGCTGACGCTGCTCATCGCCGCGGTCCTACGGTGTC AGGGCCAGGATGCCCAGGAGGCTGGCAGCTGTCTGCAGAATGGGCAGAGGTATAAAGATAAGGATGTATG GAAGCCCTCATCTTGCCGCATCTGTGTGTGTGACACTGGGAATGTCCTCTGCGATGACATTATCTGTGAA GACCCAGACTGCCTCAACCCCGAGATCCCCTTCGGAGAGTGCTGTCCCATCTGCCCAGCTGACCTCGCCA CTGCCAGTGGAAAATTAGGGCCAAAGGGGCAGAAAGGAGAACCTGGAGATATCAGAGATATCATAGGACC CAGAGGACCTCCTGGCCCTCAGGGACCTGCAGGTGAACAAGGACCCAGAGGTGATCGTGGTGACAAGGGA GAAAAGGGTGCGCCTGGACCCCGTGGCAGAGATGGAGAACCTGGTACCCCTGGAAATCCTGGCCCCGCTG GCCCTCCAGGTCCCCCTGGTCCCCCTGGCCTTAGTGCAGGAAACTTCGCGGCTCAGATGGCTGGAGGGTA TGACGAGAAGGCTGGTGGTGCCCAGATGGGAGTCATGCAAGGGCCCATGGGCCCCATGGGACCCCGTGGA CCCCCAGGCCCTGCCGGTGCCCCCGGCCCTCAAGGATTTCAAGGCAATCCTGGTGAACCTGGCGAGCCTG GTGTCTCTGGTCCCATGGGTCCCCGAGGTCCTCCTGGCCCTGCTGGAAAACCTGGTGACGACGGTGAAGC TGGGAAGCCCGGAAAGTCTGGGGAAAGAGGCCTCCCTGGCCCTCAGGGTGCTCGTGGATTCCCAGGAACC CCGGGTCTCCCCGGTGTCAAGGGTCACAGAGGTTACCCAGGCCTCGACGGTGCTAAGGGGGAAGCTGGTG CTCCGGGTGTGAAGGGTGAGAGTGGTTCCCCTGGTGAGAACGGATCCCCGGGCCCAATGGGTCCCCGTGG CCTGCCTGGTGAGAGAGGACGGACTGGCCCTGCTGGTGCTGCTGGTGCTCGGGGTAACGATGGCCAGCCA GGCCCCGCTGGACCTCCGGGTCCTGTGGGTCCCGCAGGTGGTCCTGGCTTCCCTGGTGCTCCTGGTGCCA AGGGCGAAGCTGGTCCCACTGGTGCTCGCGGTCCTGAAGGTGCTCAAGGTTCTCGTGGCGAGCCTGGCAA TCCTGGGTCCCCTGGGCCTGCAGGTGCTTCTGGTAACCCAGGGACTGATGGTATTCCTGGAGCCAAAGGA TCCGCTGGTGCTCCTGGAATTGCTGGTGCCCCTGGCTTCCCTGGGCCCCGTGGCCCTCCCGGTCCTCAAG GTGCAACTGGTCCCCTTGGCCCCAAAGGTCAGGCGGGTGAACCTGGCATTGCTGGCTTTAAAGGTGATCA AGGCCCCAAGGGAGAGACTGGACCTGCTGGGCCCCAAGGAGCCCCTGGCCCCGCTGGTGAAGAAGGCAAA CGAGGTGCTCGAGGAGAGCCGGGTGGTGCTGGACCAATCGGACCCCCTGGAGAGAGAGGTGCTCCTGGCA ACCGTGGATTCCCAGGTCAAGATGGTCTGGCAGGTCCCAAGGGTGCCCCTGGAGAGCGAGGGCCCAGTGG CTTGACTGGTCCCAAGGGAGCCAACGGTGACCCGGGTCGTCCTGGAGAACCTGGTCTTCCTGGAGCCAGG GGTCTTACCGGTCGCCCTGGTGACGCTGGTCCTCAAGGCAAAGTTGGTCCTTCTGGAGCCCCTGGTGAAG ACGGTCGCCCTGGACCTCCTGGTCCTCAGGGAGCTCGTGGGCAGCCTGGCGTCATGGGTTTCCCTGGCCC CAAAGGTGCCAACGGCGAGCCTGGCAAAGCTGGTGAGAAGGGTCTGGCTGGCGCTCCTGGTCTGAGAGGT CTTCCTGGAAAAGACGGTGAGACGGGAGCCGCAGGACCCCCCGGCCCCAGTGGACCTGCTGGTGAACGAG GCGAGCAGGGCGCTCCTGGACCATCAGGGTTCCAGGGACTTCCTGGCCCTCCCGGTCCCCCAGGTGAAGG TGGAAAGCAAGGTGACCAGGGTATTCCTGGTGAAGCTGGAGCTCCTGGCCTTGTGGGTCCTCGGGGCGAG CGAGGTTTCCCAGGTGAACGTGGCTCTCCTGGTGCTCAGGGCCTTCAGGGTCCCCGAGGCCTCCCTGGCA CTCCTGGTACTGATGGTCCCAAAGGTGCAGCTGGCCCAGATGGCCCCCCTGGGGCTCAGGGGCCTCCAGG TCTACAGGGAATGCCTGGTGAGAGAGGAGCCGCTGGCATTGCTGGGCCCAAGGGAGACAGAGGCGATGTT GGCGAGAAAGGCCCAGAGGGAGCTCCTGGGAAGGATGGCGGCCGAGGTCTGACTGGGCCCATCGGACCCC CAGGCCCAGCAGGGGCCAACGGCGAGAAGGGAGAAGTCGGACCTCCTGGCCCGTCAGGAAGTACCGGAGC TCGAGGTGCCCCGGGTGAACGCGGAGAGACCGGGCCACCTGGACCTGCTGGATTCGCTGGCCCTCCTGGT GCTGATGGCCAGCCTGGTGCCAAGGGTGATCAAGGAGAAGCCGGACAGAAAGGAGATGCTGGTGCCCCCG GCCCACAAGGCCCCTCGGGAGCCCCTGGGCCACAGGGTCCTACTGGAGTGACTGGTCCTAAGGGAGCCCG AGGTGCCCAAGGTCCCCCGGGAGCCACCGGATTCCCTGGAGCTGCTGGCCGAGTTGGACCCCCAGGTGCT AATGGCAATCCTGGACCCGCCGGTCCCCCTGGTCCTGCTGGAAAAGATGGTCCCAAAGGTGTTCGAGGAG ACAGTGGCCCCCCTGGCAGAGCTGGTGACCCCGGTCTTCAAGGTCCTGCAGGAGCTCCTGGCGAGAAAGG AGAACCTGGAGATGATGGTCCCTCTGGTCTTGATGGTCCTCCAGGTCCCCAGGGGCTGGCTGGTCAAAGG GGCATTGTTGGTCTGCCTGGTCAGCGTGGTGAGAGAGGATTCCCCGGCCTTCCCGGCCCATCGGGTGAGC CCGGCAAGCAGGGTGCACCTGGCGCGTCTGGAGACAGAGGTCCTCCTGGTCCTGTGGGGCCTCCTGGCCT GACAGGGCCTGCAGGTGAACCTGGACGAGAGGGCAGCCCTGGTGCTGATGGACCCCCTGGAAGAGATGGT GCAGCTGGAGTCAAGGGAGATCGTGGTGAGACTGGAGCACTGGGTGCCCCTGGAGCTCCTGGGCCCCCAG GCTCTCCTGGTCCTGCTGGCCCAACTGGCAAACAAGGAGACAGAGGAGAGGCTGGTGCACAAGGTCCTAT GGGTCCCTCAGGACCTGCTGGAGCCCGTGGGATTGCAGGCCCTCAAGGCCCCCGAGGTGACAAAGGAGAA TCTGGAGAGCAGGGCGAGAGGGGACTGAAGGGACACCGAGGTTTCACTGGACTGCAGGGTCTGCCTGGCC CTCCGGGTCCTTCTGGAGATCAGGGTGCTTCTGGCCCTGCTGGTCCTTCTGGCCCTAGAGGTCCACCTGG CCCTGTTGGTCCCTCTGGCAAAGATGGCTCTAATGGAATCCCTGGCCCCATCGGGCCTCCAGGTCCCCGT GGACGCTCAGGAGAAACAGGCCCTGTTGGTCCCCCTGGAAGTCCCGGTCCTCCTGGCCCTCCAGGTCCTC CTGGTCCTGGCATCGACATGTCAGCCTTTGCTGGCTTAGGGCAGAGAGAGAAGGGCCCCGACCCCATGCA GTACATGCGGGCCGACGAGGCAGACAGTACCTTGAGACAGCACGACGTGGAGGTGGACGCTACACTCAAG TCACTGAACAACCAGATTGAGAGCATCCGCAGCCCCGACGGCTCCCGCAAGAACCCTGCTCGCACTTGCC AAGACCTGAAACTCTGCCACCCCGAGTGGAAGAGCGGAGACTACTGGATTGATCCCAACCAGGGCTGCAC CTTGGACGCCATGAAAGTTTTCTGCAACATGGAGACCGGCGAGACTTGCGTCTACCCCAACCCAGCGACT GTCCCTCGGAAAAACTGGTGGAGCAGCAAGAGCAAGGAAAAGAAACACATCTGGTTTGGAGAGACCATGA ACGGTGGCTTCCACTTCAGCTATGGCGATGGCAACCTGGCTCCCAACACCGCTAACGTCCAGATGACTTT CCTCCGTCTACTGTCCACTGAGGGCTCCCAGAACATCACCTACCACTGTAAGAACAGCATCGCCTACCTG GACGAAGCGGCTGGCAACCTCAAGAAGGCCTTGCTCATCCAGGGCTCCAATGATGTAGAGATGAGGGCCG AGGGCAACAGCAGGTTCACATACACTGCCCTGAAGGATGGCTGCACGAAACACACTGGTAAGTGGGGCAA GACCGTCATCGAGTACCGATCACAGAAGACCTCCCGCCTTCCCATTATTGACATCGCACCCATGGACATT GGAGGGGCTGAACAGGAATTTGGTGTGGACATAGGGCCTGTCTGCTTCTTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG212008 representing BC082331 Red=Cloning site Green=Tags(s) MIRLGAPQSLVLLTLLIAAVLRCQGQDAQEAGSCLQNGQRYKDKDVWKPSSCRICVCDTGNVLCDDIICE DPDCLNPEIPFGECCPICPADLATASGKLGPKGQKGEPGDIRDIIGPRGPPGPQGPAGEQGPRGDRGDKG EKGAPGPRGRDGEPGTPGNPGPAGPPGPPGPPGLSAGNFAAQMAGGYDEKAGGAQMGVMQGPMGPMGPRG PPGPAGAPGPQGFQGNPGEPGEPGVSGPMGPRGPPGPAGKPGDDGEAGKPGKSGERGLPGPQGARGFPGT PGLPGVKGHRGYPGLDGAKGEAGAPGVKGESGSPGENGSPGPMGPRGLPGERGRTGPAGAAGARGNDGQP GPAGPPGPVGPAGGPGFPGAPGAKGEAGPTGARGPEGAQGSRGEPGNPGSPGPAGASGNPGTDGIPGAKG SAGAPGIAGAPGFPGPRGPPGPQGATGPLGPKGQAGEPGIAGFKGDQGPKGETGPAGPQGAPGPAGEEGK RGARGEPGGAGPIGPPGERGAPGNRGFPGQDGLAGPKGAPGERGPSGLTGPKGANGDPGRPGEPGLPGAR GLTGRPGDAGPQGKVGPSGAPGEDGRPGPPGPQGARGQPGVMGFPGPKGANGEPGKAGEKGLAGAPGLRG LPGKDGETGAAGPPGPSGPAGERGEQGAPGPSGFQGLPGPPGPPGEGGKQGDQGIPGEAGAPGLVGPRGE RGFPGERGSPGAQGLQGPRGLPGTPGTDGPKGAAGPDGPPGAQGPPGLQGMPGERGAAGIAGPKGDRGDV GEKGPEGAPGKDGGRGLTGPIGPPGPAGANGEKGEVGPPGPSGSTGARGAPGERGETGPPGPAGFAGPPG ADGQPGAKGDQGEAGQKGDAGAPGPQGPSGAPGPQGPTGVTGPKGARGAQGPPGATGFPGAAGRVGPPGA NGNPGPAGPPGPAGKDGPKGVRGDSGPPGRAGDPGLQGPAGAPGEKGEPGDDGPSGLDGPPGPQGLAGQR GIVGLPGQRGERGFPGLPGPSGEPGKQGAPGASGDRGPPGPVGPPGLTGPAGEPGREGSPGADGPPGRDG AAGVKGDRGETGALGAPGAPGPPGSPGPAGPTGKQGDRGEAGAQGPMGPSGPAGARGIAGPQGPRGDKGE SGEQGERGLKGHRGFTGLQGLPGPPGPSGDQGASGPAGPSGPRGPPGPVGPSGKDGSNGIPGPIGPPGPR GRSGETGPVGPPGSPGPPGPPGPPGPGIDMSAFAGLGQREKGPDPMQYMRADEADSTLRQHDVEVDATLK SLNNQIESIRSPDGSRKNPARTCQDLKLCHPEWKSGDYWIDPNQGCTLDAMKVFCNMETGETCVYPNPAT VPRKNWWSSKSKEKKHIWFGETMNGGFHFSYGDGNLAPNTANVQMTFLRLLSTEGSQNITYHCKNSIAYL DEAAGNLKKALLIQGSNDVEMRAEGNSRFTYTALKDGCTKHTGKWGKTVIEYRSQKTSRLPIIDIAPMDI GGAEQEFGVDIGPVCFL TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC082331
ORF Size	4463 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC082331, AAH82331
RefSeq Size	5082 bp
RefSeq ORF	4463 bp
Locus ID	12824
Gene Summary	This gene encodes the alpha-1 subunit of the fibril-forming type II collagen, the major component of cartilage and the vitreous humor of the eye. The encoded preproprotein forms homotrimeric, triple helical procollagen that undergoes proteolytic processing during fibirl formation. Mice harboring certain mutations in this gene exhibit severe chondrodysplasia characterized by short limbs and trunch, craniofacial deformities and cleft palate. A complete lack of the encoded protein in mice results in postnatal lethality. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar proteolytic processing. [provided by RefSeq, Dec 2015]

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