Eif2ak4 (BC072637) Mouse Tagged ORF Clone

CAT#: MG212025

  • TrueORF®

Eif2ak4 (tGFP-tagged) - Mouse eukaryotic translation initiation factor 2 alpha kinase 4 (cDNA clone MGC:100018 IMAGE:30354433), complete



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Mouse Protein / Lysate Request

CNY 13,300.00


货期*
5周

规格
    • 10 ug

Cited in 3 publications.

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms GCN2, MGCN2, 2610011M03
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG212025 representing BC072637
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCCACCTACATACCCAGATGTGTGATGATATTTGAACTGGCACACCATGTGCAGTCGTTTCTCAGCG
AGCATAACAAGCCCCCACCAAAGTCTTTCCATGAAGAAATGCTGGAAAGGCAGGCTCAGGAGAAGCAGCA
GAGGTTGCTGGAGGCCAGGCGGAAGGAGGAGCAGGAGCAACGTGAAATCCTCCACGAGATTCAGAGAAGG
AAAGAGGAGATCAAGGAAGAGAAGAAAAGGAAGGAAATGGCTAAGCAGGAACGTTTGGAAATCACTAGCT
TGACAAACCAGGACTATGCCTCTAAGAGAGACCCAGCAGGGCACAGGGCAGCTGCCATCCTCCATGGAGG
CTCTCCTGACTTTGTAGGAAATGGTAAAGCCCGGACATACTCCTCAGGAAGGTCCAGGCGAGAACGTCAG
TATTCTGTGTGTAGTGGTGAACCCTCTCCTGGCTCGTGTGACATCCTCCATTTCTCTGTGGGTAGCCCTG
ATCAGCTCATGGTGCACAAAGGGAGATGTGTTGGCAGTGATGAGCAGCTGGGAAAGGTGGTATACAATGC
TTTGGAAACAGCCACGGGCAGCTTTGTCTTGCTGCATGAGTGGGTCCTTCAATGGCAGAAGATGGGCCCT
TGCCTTACCAGTCAAGAGAAAGAGAAGATTGACAAGTGCAAAAGGCAGATTCAAGGAGCAGAAACAGAAT
TCAGCTCCCTAGTGAAACTGAGCCATCCAAATATCGTCCGCTACTTTGCGATGAACTCCAGAGAGGAGGA
GGACTCCATTGTGATCGACATTCTGGCAGAGCACGTCAGTGGCATCTCTCTGGCTACACACCTGAGCCAC
TCGGGCCCAGTCCCCGCCCACCAGCTGCGCAAGTACACAGCCCAGCTCCTGGCAGGCCTGGATTACCTAC
ACAGCAACTCCGTGGTGCACAAGGTTCTGAGCGCGTCCAGTGTCTTGGTAGATGCCGAGGGCACTGTCAA
GATAACAGACTACAGCATCTCTAAGCGTCTGGCAGACATTTGCAAGGAGGATGTATTTGAGCAAGCTCGA
GTTCGTTTTAGTGACAGTGCCCTGCCTTATAAAACAGGAAAGAAAGGGGATGTGTGGCGGCTCGGCCTCC
TGCTGTTGTCTCTCAGCCAAGGACAGGAGTGTGGGGAGTATCCGGTGACCATCCCCAGTGACCTGCCAGC
TGACTTCCAAGACTTCCTGAAGAAGTGTGTCTGCCTGGACGACAAGGAAAGATGGAGCCCTCAGCAGCTG
CTGAAACACAGCTTTATAAACCCTCAACCAAAGCTGCCTTTGGTGGAGCAGAGTCCTGAAGACTCTGGGG
GACAAGACTACATTGAGACCGTCATTCCCAGCAACCAGCTGCCCAGCGCTGCGTTCTTCAGTGAGACACA
GAAACAGTTCTCCCGGTACTTCATTGAGTTTGAAGAACTACAACTCCTAGGGAAAGGAGCTTTTGGAGCT
GTCATCAAGGTGCAAAACAAGCTTGATGGCTGCTGCTATGCTGTGAAGCGCATCCCTATCAACCCTGCAA
GCAGACACTTCCGCCGGATCAAGGGCGAGGTGACACTGCTATCGCGCCTGCACCATGAGAACATTGTGCG
CTACTACAACGCCTGGATCGAGCGGCATGAGCGCCCAGCGGTGCCAGGGACACCGCCCCCAGACTGCACA
CCCCAGGCCCAGGACAGCCCAGCCACCTGCGGGAAAACATCAGGCGACACTGAAGAACTGGGCAGCGTGG
AGGCTGCAGCACCGCCACCCATCCTCAGCAGCTCGGTGGAGTGGAGCACATCTGCAGAGCGTTCTACCAG
CACCCGCTTCCCAGTCACGGGCCAGGATTCCAGCAGCGATGAGGAGGACGAGGACGAGCGCGATGGCGTC
TTCTCCCAGTCCTTTCTACCTGCTTCAGATTCTGACAGTGACATCATCTTTGACAATGAAGATGAAAACA
GTAAAAGTCAAAATCAGGATGAAGACTGCAATCAAAAGGACGGTAGCCATGAGATTGAGCCTTCAGTGAC
AGCTGAGGCTGTGCACTACCTTTATATCCAGATGGAGTACTGCGAGAAGAGCACGCTGCGGGACACCATT
GACCAGGGGCTGTTCCGAGACACCAGCCGGCTCTGGAGGCTTTTCCGAGAGATTCTGGATGGATTAGCTT
ATATCCATGAGAAAGGAATGATTCATCGCGACTTGAAGCCTGTCAATATTATTTTGGATTCTGATGACCA
TGTGAAAATAGGTGACTTTGGCCTGGCAACAGACCATCTGGCCTTCACTGCTGAAGGTAAACAGGACGAC
CAGGCAGGTGACGGAGTGATTAAGTCAGACCCTTCAGGCCATTTGACTGGCATGGTTGGTACTGCTCTGT
ATGTAAGCCCTGAGGTCCAAGGAAGCACCAAGTCTGCATACAACCAGAAAGTGGATCTCTTCAGCCTGGG
AATTATCTTCTTTGAGATGTCTTATCACCCGATGGTGACTGCCTCAGAAAGAATTTTCGTTCTCAACCAA
CTTCGAGATCCCACATCGCCCAAGTTTCCAGACGACTTCGATGATGGAGAACATACAAAGCAGAAATCTG
TCATCTCCTGGCTGTTGAACCATGATCCAGCTAAACGGCCCACGGCCATGGAACTGCTCAAGAGTGAGCT
GCTCCCCCCGCCGCAGATGGAGGAGTCCGAGCTGCATGAAGTGCTGCACCACACGCTGGCCAACATAGAT
GGGAAGGCCTACCGCACCATGATGAGTCAGATCTTCTGCCAGCACATCTCCCCCGCTATAGACTACACCT
ACGACAGTGACATCCTGAAGGGCAACTTCTTGATTCGTACAGCCAAGATCCAGCAGCTTGTATGTGAAAC
CATCGTCCGCGTCTTTAAAAGGCATGGCGCTGTCCAGCTGTGCACCCCACTGCTGCTTCCCCGAAACAGG
CAAATATATGAGCACAACGAAGCGGCTTTGTTCATGGATCACAGCGGCATGCTGGTGATGCTTCCGTTCG
ACCTGCGGGTCCCTTTTGCAAGATATGTGGCAAGAAATAACATACTGAATTTAAAACGATACTGCATAGA
GCGGGTGTTCAGACCTCGGAAGTTAGACCGATTTCATCCCAAAGAACTCCTGGAATGTGCATTTGATATC
GTCACTTCTACCACCAACAGCTCTCTGCCCACTGCCGAAACCATCTACACCATCTATGAAATAATCCAGG
AGTTTCCTGCACTTCAGGAAAGGAATTACAGCATTTACTTGAACCATACGATGCTTCTGAAGGCAATACT
CCTACACTGTGGGATCCCAGAGGACAAGCTCAGCCAAGTCTACGTCATTCTGTATGATGCTGTGACAGAG
AAGCTGACTAGGAGAGAAGTGGAAGCTAAATTCTGTAACCTGTCCTTGTCTTCTAATAGCCTGTGTAGAC
TCTACAAGTTCATTGAGCAGAAGGGGGATTTGCAAGACTTAACGCCAACCATCAACTCACTAATAAAACA
GAAAACAGGCGTTGCTCAGTTGGTGAAGTATAGCTTAAAAGACCTCGAGGACGTCGTCGGGCTGCTGAAG
AAACTTGGCGTGAAGTTACAGGTCTCCATCAACCTGGGCTTGGTCTACAAGGTGCAGCAGCACACTGGCA
TCATCTTCCAGTTCCTGGCATTCAGCAAACGCAGGCAAAGGGTTGTGCCTGAGATCCTTGCCGCCGGTGG
CAGATACGACCTGCTGATTCCCAAGTTCAGAGGCCCACAGACTGTGGGGCCAGTCCCCACTGCTGTCGGT
GTCAGCATAGCCATAGACAAGATATTTGCTGCTGTCCTCAACATGGAAGAGCCTGTTACAGTGAGCTCCT
GTGACCTCCTGGTTGTCAGTGTTGGCCAGATGTCCATGTCCAGGGCCATCAACCTAACCCAGAAACTGTG
GACAGCGGGCATCACTGCAGAAATCATGTATGACTGGTCCCAGTCCCAGGAAGAGTTACAAGAGTACTGC
AGACACCATGAAATCACCTATGTGGCTCTGGTCTCCGATAAAGAAGGAAGCCATGTCAAGGTCAAGTCCT
TTGAGAAGGAGAGGCAAACAGAAAAGCGTGTATTGGAATCGGATCTTGTGGATCACGTTATGCAGAAACT
AAGAACCAAAGTTGGTGACGAAAGAAATTTCAGAGACGCTTCTGATAATCTTGCCGTACAAACTCTGAAG
GGGTCGTTTTCTAATGCTTCAGGTTTGTTTGAAATCCATGGAACTACAGTAGTCCCCAACGTGATTGTTC
TAGCACCAGAAAAGCTGTCAGCCAGCACCAGGAGGCGACATGAGATTCAGGTGCAAACCCGACTTCAGAC
TACACTTGCCAACTTACATCAGAAAAGCAGTGAAATTGAAATTTTGGCTGTGGACCTACCCAAGGAAACA
ATCTTACAGTTTCTATCATTAGAGTGGGATGCTGATGAACAGGCATTTAACACAACTGTGAAGCAGCTGC
TGTCACGCCTGCCAAAGCAAAGATACCTCAAACTCGTCTGCGATGAAATTTATAACATCAAAGTTGAAAA
GAAGGTGTCAGTGCTGTTCCTGTACAGCTACAGAGATGACTACTACAGAATCCTATTT


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG212025 representing BC072637
Red=Cloning site Green=Tags(s)

MPTYIPRCVMIFELAHHVQSFLSEHNKPPPKSFHEEMLERQAQEKQQRLLEARRKEEQEQREILHEIQRR
KEEIKEEKKRKEMAKQERLEITSLTNQDYASKRDPAGHRAAAILHGGSPDFVGNGKARTYSSGRSRRERQ
YSVCSGEPSPGSCDILHFSVGSPDQLMVHKGRCVGSDEQLGKVVYNALETATGSFVLLHEWVLQWQKMGP
CLTSQEKEKIDKCKRQIQGAETEFSSLVKLSHPNIVRYFAMNSREEEDSIVIDILAEHVSGISLATHLSH
SGPVPAHQLRKYTAQLLAGLDYLHSNSVVHKVLSASSVLVDAEGTVKITDYSISKRLADICKEDVFEQAR
VRFSDSALPYKTGKKGDVWRLGLLLLSLSQGQECGEYPVTIPSDLPADFQDFLKKCVCLDDKERWSPQQL
LKHSFINPQPKLPLVEQSPEDSGGQDYIETVIPSNQLPSAAFFSETQKQFSRYFIEFEELQLLGKGAFGA
VIKVQNKLDGCCYAVKRIPINPASRHFRRIKGEVTLLSRLHHENIVRYYNAWIERHERPAVPGTPPPDCT
PQAQDSPATCGKTSGDTEELGSVEAAAPPPILSSSVEWSTSAERSTSTRFPVTGQDSSSDEEDEDERDGV
FSQSFLPASDSDSDIIFDNEDENSKSQNQDEDCNQKDGSHEIEPSVTAEAVHYLYIQMEYCEKSTLRDTI
DQGLFRDTSRLWRLFREILDGLAYIHEKGMIHRDLKPVNIILDSDDHVKIGDFGLATDHLAFTAEGKQDD
QAGDGVIKSDPSGHLTGMVGTALYVSPEVQGSTKSAYNQKVDLFSLGIIFFEMSYHPMVTASERIFVLNQ
LRDPTSPKFPDDFDDGEHTKQKSVISWLLNHDPAKRPTAMELLKSELLPPPQMEESELHEVLHHTLANID
GKAYRTMMSQIFCQHISPAIDYTYDSDILKGNFLIRTAKIQQLVCETIVRVFKRHGAVQLCTPLLLPRNR
QIYEHNEAALFMDHSGMLVMLPFDLRVPFARYVARNNILNLKRYCIERVFRPRKLDRFHPKELLECAFDI
VTSTTNSSLPTAETIYTIYEIIQEFPALQERNYSIYLNHTMLLKAILLHCGIPEDKLSQVYVILYDAVTE
KLTRREVEAKFCNLSLSSNSLCRLYKFIEQKGDLQDLTPTINSLIKQKTGVAQLVKYSLKDLEDVVGLLK
KLGVKLQVSINLGLVYKVQQHTGIIFQFLAFSKRRQRVVPEILAAGGRYDLLIPKFRGPQTVGPVPTAVG
VSIAIDKIFAAVLNMEEPVTVSSCDLLVVSVGQMSMSRAINLTQKLWTAGITAEIMYDWSQSQEELQEYC
RHHEITYVALVSDKEGSHVKVKSFEKERQTEKRVLESDLVDHVMQKLRTKVGDERNFRDASDNLAVQTLK
GSFSNASGLFEIHGTTVVPNVIVLAPEKLSASTRRRHEIQVQTRLQTTLANLHQKSSEIEILAVDLPKET
ILQFLSLEWDADEQAFNTTVKQLLSRLPKQRYLKLVCDEIYNIKVEKKVSVLFLYSYRDDYYRILF

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC072637
ORF Size 4610 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC072637, AAH72637
RefSeq Size 4951 bp
RefSeq ORF 4610 bp
Locus ID 27103
Gene Summary Metabolic-stress sensing protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (eIF-2-alpha/EIF2S1) on 'Ser-52' in response to low amino acid availability (PubMed:10504407, PubMed:10655230, PubMed:12176355, PubMed:12215525, PubMed:15213227, PubMed:16054071, PubMed:16176978, PubMed:16121183, PubMed:15774759, PubMed:16601681, PubMed:26102367). Plays a role as an activator of the integrated stress response (ISR) required for adapatation to amino acid starvation. Converts phosphorylated eIF-2-alpha/EIF2S1 either to a competitive inhibitor of the translation initiation factor eIF-2B, leading to a global protein synthesis repression, and thus to a reduced overall utilization of amino acids, or to a translational initiation activation of specific mRNAs, such as the transcriptional activator ATF4, and hence allowing ATF4-mediated reprogramming of amino acid biosynthetic gene expression to alleviate nutrient depletion (PubMed:10655230, PubMed:11106749, PubMed:12176355, PubMed:15213227, PubMed:16176978, PubMed:26102367). Binds uncharged tRNAs (By similarity). Involved in cell cycle arrest by promoting cyclin D1 mRNA translation repression after the unfolded protein response pathway (UPR) activation or cell cycle inhibitor CDKN1A/p21 mRNA translation activation in response to amino acid deprivation (PubMed:16176978, PubMed:26102367). Plays a role in the consolidation of synaptic plasticity, learning as well as formation of long-term memory (PubMed:16121183). Plays a role in neurite outgrowth inhibition (PubMed:23447528). Plays a role in feeding behavior to maintain amino acid homeostasis; contributes to the innate aversion toward diets of imbalanced amino acid composition (PubMed:16054071, PubMed:15774759). Plays a proapoptotic role in response to glucose deprivation (PubMed:20660158). Promotes global cellular protein synthesis repression in response to UV irradiation independently of the stress-activated protein kinase/c-Jun N-terminal kinase (SAPK/JNK) and p38 MAPK signaling pathways (PubMed:12176355).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (3)

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