Bai2 (BC056926) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	Bai2
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG212029 representing BC056926 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCCCAGCCTGTCCCCTCTTACTGTCTGTGATTCTGTCCCTGCGCCTGGCCACGGCCTTCGACCCTG CCCCCAGTGCCTGCTCTGCCCTGGCCTCGGGCGTGCTCTACGGGGCCTTCTCGCTGCAGGACCTCTTTCC CACCATCGCCTCGGGCTGCTCCTGGACCCTGGAGAACCCAGACCCCACCAAGTACTCCCTCTACCTGCGC TTCAACCGGCAGGAGCAGGTTTGCACACACTTTGCCCCGCGCCTGCTGCCCCTGGACCACTACCTGGTCA ACTTTACCTGCCTGCGGCCTGGTCCAGAGGAAGCCACAGCCCGGGCTGAGTCGGAGGTGGGACGGCCAGA GGAGGAGGAGGAGGAGGCGGCGGCAGCAGCATCAGGGTTGGAGTTGTGTGGTGGCTCAGGCCCCTTTACC TTTCTGCACTTCGACAAGAACTTTGTGCAGCTGTGCCTGTCGGCTGAGCCCTCTGAGGCCCCTCGTCTGC TAGCGCCTGCTGCCCTGGCCTTCCGTTTTGTGGAGGTCTTGCTGATCAACAACAACAACTCCAGCCAGTT CACCTGTGGTGTGCTCTGCCGCTGGAGTGAGGAGTGTGGCCGGGCTGCAGGCAGGGCTTGTGGCTTTGCA CAGCCAGGGTGTAGTTGTCCTGGGGAGGCAGGGGCCAACCCCGCCACCACCACATCTCCGGGGCCTCCGG TTGCCCACACCCTGTCCAATGCCCTGGTGCCCGGGGGCCCAGCCCCTCCTGCTGAGGCCGACTTGCACTC GGGGAGCAGCAATGACCTGTTCACCACCGAGATGAGATATGGTGAGGAGCCGGAAGAGGAACCGAAGGTG AAAACCCAGTGGCCAAGGTCTGCAGATGAGCCTGGGCTATACATGGCGCAGACAGGCGACCCAGCAGCTG AGGAGTGGTCCCCGTGGAGCGTGTGTTCCCTGACGTGTGGGCAGGGTCTGCAGGTGCGGACCCGCTCCTG CGTGTCCTCCCCCTATGGGACCCTGTGCAGCGGGCCCCTTCGGGAGACCCGGCCTTGCAACAATTCAGCC ACCTGCCCAGTGCACGGCGTGTGGGAGGAGTGGGGGTCCTGGAGCCTGTGCTCCCGCAGCTGCGGGCGGG GGTCTCGGAGCCGGATGCGGACCTGCGTGCCCCCCCAGCACGGCGGCAAGGCCTGCGAGGGTCCCGAGCT GCAGACTAAACTCTGCAGTATGGCCGCCTGCCCGGTGGAAGGCCAGTGGCTAGAATGGGGTCCCTGGGGC CCATGCTCATCATCTTGTGCCAATGGGACCCAGCAGCGCAGCCGGAAATGCAGTGTGGCGGGTCCAGCCT GGGCCACGTGCGCAGGTGCCCTCACGGATACCCGTGAGTGCAGCAATCTCGATTGCCCGGCCACTGACGG CAAGTGGGGGCCGTGGAACGCGTGGAGCCTGTGCTCCAAGACGTGTGACACGGGCTGGCAACGCCGCTTC CGCATGTGCCAGGCTTCTGGCACACAGGGCTACCCTTGCGAGGGCACAGGAGAGGAGGTGAAACCCTGCA GTGAGAAGAGGTGTCCAGCCTTCCATGAGATGTGCAGGGATGAGTACGTGATGTTGATGACATGGAAGAG GGCGGCAGCTGGCGAGATCATTTACAACAAGTGTCCCCCTAATGCCTCGGGTTCTGCTAGCCGCCGCTGT CTCCTCAGTGCCCAGGGCGTAGCATACTGGGGACTGCCCAGCTTTGCTCGTTGCATATCCCATGAATACC GCTACCTGTACCTGTCACTTCGGGAACACCTGGCTAAGGGCCAGCGCATGCTGGCAGGTGAGGGCATGTC ACAGGTGGTGCGGAGCCTGCAGGAGCTACTGGCACGGCGCACTTACTACAGCGGGGACCTGCTCTTCTCT GTGGACATCCTAAGGAACGTCACTGACACCTTCAAGAGGGCCACCTATGTCCCTTCCGCCGATGACGTGC AGCGTTTCTTCCAGGTGGTGAGCTTCATGGTGGATTCAGAAAACAAGGACAAATGGGATGATGCTCAGCA GGTGTCACCGGGCTCTGTGCACCTGCTGCGTGTTGTGGAAGATTTCATTCACCTCGTGGGCGACGCTCTC AAGGCCTTCCAGAGCTCTCTCATTGTCACGGACAATCTGGTGATCAGCATTCAGAGAGAGCCTATCTCCG CCGTGTCCAGTGACATCACGTTTCCCATGCGGGGCCGCAGGGGCATGAAGGACTGGGTGCGACACTCAGA GGATCGTCTCTTTCTACCCAAGGAGGTGCTCAGCCTGTCCTCCCCAGGAAAGCCAGCCACACCTGGGGCA GCCACAGCAGGCAGCCCGGGCAGGGGGAGGGGCCCAGGAACGGTGCCCCCTGGCCCAGGCCACGCCCACC AGCGCCTTCTCCCAGCTGACCCCGAAGAGTCCTCCTCCTACTTTGTGATCGGTGCTGTGCTCTACCGCAC CCTTGGCCTCATCCTGCCGCCCCCCAGGCCTCCACTTGCTGTCACCTCCCGGGTGATGACAGTGACTGTG CGTCCCCCCACCCAGCCTCCAGCTGAGCCCCTCATTACAGTGGAACTCTCGTACATCATCAATGGCACCA CCGATCCCCACTGTGCCAGCTGGGACTACTCCAGAGCAGATACCAACTCGGGGGACTGGAACACTGAGAG CTGCCAGACCTTGGAGACCCAGGCGGCTCACACCCGCTGCCAGTGCCAGCACCTGTCCACCTTTGCCGTC CTGGCCCAGCCACCCAAGGACCTGACCCTGGAGCTGGCAGGTGCTCCCTCTGTCCCCCTGGTGATCGGCT GTGCAGTGTCCTGCATGGCTCTGCTCACCCTGCTGGCCATCTATGCAGCCTTCTGGAGGTTTATAAAATC AGAACGCTCCATCATCTTGCTGAACTTCTGCCTGTCCATCCTGGCTTCCAACATTCTGATCCTGGTGGGC CAGTCCCGGGTGCTGAGCAAGGGCGTATGCACCATGACGGCTGCCTTCCTACACTTCTTCTTTCTGTCCT CCTTTTGCTGGGTGCTTACAGAGGCCTGGCAATCCTATCTGGCTGTCATCGGGCGGATGCGCACCCGCCT GGTTCGCAAGCGCTTCCTCTGCCTGGGCTGGGGTCTACCTGCCCTGGTGGTGGCTGTGTCTGTTGGCTTT ACTCGCACCAAAGGATATGGTACATCCAGCTACTGCTGGCTGTCCCTAGAGGGCGGCCTGCTCTATGCCT TCGTGGGTCCAGCAGCAGTCATTGTCCTGGTGAACATGCTCATCGGGATTATCGTCTTCAACAAGCTCAT GGCTCGCGATGGCGTCTCAGACAAATCTAAGAAGCAGAGGGCTGGCCGACTGTCTTGGAATCTCTGGGGA TATGGCTCTCAACTGTGTCTTTTTCCAAAGCTTCCAAGGGCTTCACTCTGGAGTTCCTGCGTGGTACTGC CTCTCCTGGCGCTTACCTGGATGTCTGCCGTCCTGGCCATGACAGATCGCCGCTCCGTCCTCTTCCAGGC ACTCTTTGCCGTTTTCAACTCTGCACAAGGCTTTGTCATCACCGCTGTGCACTGCTTCCTGCGCCGAGAG GTCCAGGATGTGGTAAAGTGTCAGATGGGTGTGTGTCGGGCTGATGAGAGTGAAGACTCCCCAGACTCGT GCAAGAACGGGCAGCTGCAGATCCTGTCAGACTTTGAAAAGGACGTGGATCTGGCTTGTCAGACAGTTCT GTTCAAGGAGGTCAACACCTGCAACCCGTCCACCATTACCGGCACTCTGTCCCGCCTGTCTCTGGATGAG GATGAGGAGCCCAAGTCCTGTCTCGTGGGTCCTGAGGGTGGCCTCAGCTTCTCACCACTGCCTGGGAACA TCCTGGTACCCATGGCAGCCTCACCAGGTCTAGGGGAGCCACCACCACCCCAGGAGACCAACCCTGTGTA CATGTGTGGGGAGGGTGGCCTGCGGCAGTTGGACCTTACATGGATACGGCAGAGTGAACCAGGCTCTGAG GGGGACTACATGGTTCTGCCCCGGCGGACTTTGAGCCTGCAGCCTGGTGGTGGGGGTACAGCGGGTGAGG AGGCCCCAAGGGCCCGGCCTGAGGGGACCCCCCGGCGGGCTGCCAAAACGGTAGCCCACACTGAAGGCTA CCCCAGCTTCCTGTCTGTGGAGCACTCGGGTCTAGGGCTGGGCCCTGCCTATGGGTCTCTCCAGAACCCG TATGGAATGACCTTCCAACCACCACCACCAACACCCAGCGCCCGCCAAGTACCAGAGCCAGGAGAACGTA GCCGGACCATGCCCCGTACAGTGCCTGGTTCCACCATGAAGCTGGGCTCCCTTGAGCGAAAGAAGCTTCG GTATTCGGACTTGGACTTTGAGAAGGTGATGCACACTCGGAAACGGCACTCGGAACTCTACCACGAACTC AACCAGAAGTTCCACACTTTCGACCGCTACCGTAGCCAGTCCTCAGCCAAGGAGAAACCCAGCCCCCCCG GGGGACGCCCTGGCTTGTCCCAGCACAGGAGGCATCAAAGCTGGAGCACCTTCAAATCTATGACACTGGG CTCACTGCCCCCCAAGCCCCGAGAACGGCTGGCCCTGCACCGGACAGCAGCCTGGGAGCCCACAGAACCG CCAGACGGCGACTTCCAGACAGAGGTG AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG212029 representing BC056926 Red=Cloning site Green=Tags(s) MTPACPLLLSVILSLRLATAFDPAPSACSALASGVLYGAFSLQDLFPTIASGCSWTLENPDPTKYSLYLR FNRQEQVCTHFAPRLLPLDHYLVNFTCLRPGPEEATARAESEVGRPEEEEEEAAAAASGLELCGGSGPFT FLHFDKNFVQLCLSAEPSEAPRLLAPAALAFRFVEVLLINNNNSSQFTCGVLCRWSEECGRAAGRACGFA QPGCSCPGEAGANPATTTSPGPPVAHTLSNALVPGGPAPPAEADLHSGSSNDLFTTEMRYGEEPEEEPKV KTQWPRSADEPGLYMAQTGDPAAEEWSPWSVCSLTCGQGLQVRTRSCVSSPYGTLCSGPLRETRPCNNSA TCPVHGVWEEWGSWSLCSRSCGRGSRSRMRTCVPPQHGGKACEGPELQTKLCSMAACPVEGQWLEWGPWG PCSSSCANGTQQRSRKCSVAGPAWATCAGALTDTRECSNLDCPATDGKWGPWNAWSLCSKTCDTGWQRRF RMCQASGTQGYPCEGTGEEVKPCSEKRCPAFHEMCRDEYVMLMTWKRAAAGEIIYNKCPPNASGSASRRC LLSAQGVAYWGLPSFARCISHEYRYLYLSLREHLAKGQRMLAGEGMSQVVRSLQELLARRTYYSGDLLFS VDILRNVTDTFKRATYVPSADDVQRFFQVVSFMVDSENKDKWDDAQQVSPGSVHLLRVVEDFIHLVGDAL KAFQSSLIVTDNLVISIQREPISAVSSDITFPMRGRRGMKDWVRHSEDRLFLPKEVLSLSSPGKPATPGA ATAGSPGRGRGPGTVPPGPGHAHQRLLPADPEESSSYFVIGAVLYRTLGLILPPPRPPLAVTSRVMTVTV RPPTQPPAEPLITVELSYIINGTTDPHCASWDYSRADTNSGDWNTESCQTLETQAAHTRCQCQHLSTFAV LAQPPKDLTLELAGAPSVPLVIGCAVSCMALLTLLAIYAAFWRFIKSERSIILLNFCLSILASNILILVG QSRVLSKGVCTMTAAFLHFFFLSSFCWVLTEAWQSYLAVIGRMRTRLVRKRFLCLGWGLPALVVAVSVGF TRTKGYGTSSYCWLSLEGGLLYAFVGPAAVIVLVNMLIGIIVFNKLMARDGVSDKSKKQRAGRLSWNLWG YGSQLCLFPKLPRASLWSSCVVLPLLALTWMSAVLAMTDRRSVLFQALFAVFNSAQGFVITAVHCFLRRE VQDVVKCQMGVCRADESEDSPDSCKNGQLQILSDFEKDVDLACQTVLFKEVNTCNPSTITGTLSRLSLDE DEEPKSCLVGPEGGLSFSPLPGNILVPMAASPGLGEPPPPQETNPVYMCGEGGLRQLDLTWIRQSEPGSE GDYMVLPRRTLSLQPGGGGTAGEEAPRARPEGTPRRAAKTVAHTEGYPSFLSVEHSGLGLGPAYGSLQNP YGMTFQPPPPTPSARQVPEPGERSRTMPRTVPGSTMKLGSLERKKLRYSDLDFEKVMHTRKRHSELYHEL NQKFHTFDRYRSQSSAKEKPSPPGGRPGLSQHRRHQSWSTFKSMTLGSLPPKPRERLALHRTAAWEPTEP PDGDFQTEV SGPTRTRRLE - GFP Tag - V
Restriction Sites	SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map
ACCN	BC056926
ORF Size	4649 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC056926, AAH56926
RefSeq Size	5155 bp
RefSeq ORF	4649 bp
Locus ID	230775
Gene Summary	Orphan G-protein coupled receptor involved in cell adhesion and probably in cell-cell interactions. Activates NFAT-signaling pathway, a transcription factor, via the G-protein GNAZ. Involved in angiogenesis inhibition (PubMed:12218411).[UniProtKB/Swiss-Prot Function]

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