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Myh2 (BC008538) Mouse Tagged ORF Clone
CAT＃: MG212047
- TrueORF®
Myh2 (tGFP-tagged) - Mouse myosin, heavy polypeptide 2, skeletal muscle, adult (cDNA clone MGC:7639 IMAGE:3495412)
ORF Plasmid: DDK
"BC008538" in other vectors (4)
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CNY 17,580.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Myh2a, MHC2A, MyHC-IIa |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG212047 representing BC008538
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCTCCGACGCCGAGATGGCCGTTTTCGGGGAGGCTGCCCCTTACCTCCGGAAGTCCGAAAAGGAGC GAATCGAGGCCCAGAATAGGCCTTTTGATGCCAAAACATCTGTCTTTGTGGCGGAGCCCAAGGAATCCTT TGTCAAAGGAACCATTCAGAGCAAAGATGCAGGGAAAGTGACTGTGAAAACAGAAGCAGGAGCGACCCTG ACTGTGAAAGAAGACCAGATCTTCCCCATGAACCCTCCCAAGTACGACAAGATCGAGGACATGGCCATGA TGACCCACCTGCACGAGCCCGCTGTGCTGTACAACCTCAAAGAGCGTTATGCAGCCTGGATGATCTACAC CTACTCAGGCCTCTTCTGTGTCACCGTCAACCCTTACAAATGGCTGCCGGTGTACAACCCCGAGGTGGTG GCAGCCTATCGAGGCAAAAAGCGCCAGGAGGCCCCGCCCCACATCTTCTCCATCTCTGATAACGCCTACC AGTTCATGCTAACAGACAGGGAGAATCAGTCAATCCTGATCACCGGAGAATCCGGGGCCGGGAAGACTGT GAACACGAAGCGTGTCATCCAGTACTTTGCAACAATTGCAGTCACTGGGGACAAGAAGAAGGAGGAGGCA ACTTCTGGCAAAATGCAGGGGACACTGGAGGACCAAATCATCAGCGCCAACCCCCTGCTGGAGGCCTTTG GGAACGCCAAGACCGTGAGGAACGACAACTCGTCTCGCTTTGGTAAATTCATCAGGATCCACTTTGGCAC TACGGGGAAACTGGCATCTGCCGACATCGAGACCTATCTGCTAGAGAAGTCCCGGGTCACTTTCCAGCTT AAGGCGGAAAGAAGCTACCATATATTTTATCAGATCACATCCAACAAGAAGCCAGAACTGATCGAAATGC TGCTGATCACCACGAACCCATATGATTACCCGTTTGTCAGCCAGGGAGAGATCAGTGTGGCCAGCATTGA TGACCAGGAAGAGCTGATGGCCACTGATAGTGCTATCGACATTTTGGGCTTTACAAATGATGAAAAAGTC TCCATCTATAAGCTCACCGGTGCTGTGATGCATTATGGGAACATGAAGTTCAAGCAGAAGCAGCGGGAGG AGCAGGCAGAGCCAGATGGCACCGAAGTTGCTGACAAGGCTGCCTATCTTCAGGGTCTGAACTCTGCTGA CCTGCTCAAAGCCCTCTGCTACCCCAGGGTCAAGGTCGGCAATGAGTATGTCACCAAAGGCCAGACTGTC GAGCAGGTGACCAATGCTGTGGGTGCCCTGGCCAAGGCCATGTATGAGAAGATGTTCCTGTGGATGGTCA CCCGCATCAACCAGCAGCTGGACACCAAGCAGCCCCGGCAGTACTTCATCGGGGTCTTGGACATCGCTGG CTTTGAGATCTTTGATTTCAACAGCTTGGAACAGCTGTGCATCAACTTCACCAATGAGAAACTGCAACAG TTTTTCAACCACCACATGTTCGTGCTGGAGCAGGAGGAATACAAGAAGGAGGGCATCGAGTGGACCTTCA TCGACTTCGGGATGGACCTGGCAGCCTGCATCGAGCTCATCGAGAAGCCGATGGGCATCTTCTCCATCCT GGAAGAGGAGTGCATGTTCCCTAAGGCGACAGACACCTCCTTCAAGAACAAGCTGTATGAGCAGCATCTT GGAAAGTCTGCCAACTTCCAGAAGCCTAAGGTGGTCAAAGGCAAGGCCGAAGCCCACTTCTCCCTCATCC ACTATGCGGGCACCGTGGATTACAACATCACTGGCTGGCTGGACAAGAACAAGGACCCACTGAACGAGAC CGTGGTGGGGCTGTACCAGAAGTCTTCAGTGAAAACTCTGGCTTATCTCTTCTCTGGGGCACAAACTGCT GAAGCAGAGGCAAGTAGTGGTGGAGCTGCCAAGAAAGGTGCCAAGAAGAAGGGGTCCTCTTTTCAGACCG TGTCTGCCCTGTTCAGAGAGAATTTGAACAAGCTGATGACCAACCTCAGGAGCACCCATCCTCATTTTGT GAGGTGTATCATCCCCAATGAAACCAAGACTCCCGGTGCCATGGAGCACGAACTGGTCCTGCACCAGCTG AGGTGTAATGGGGTGCTGGAGGGCATCCGCATCTGCAGGAAGGGCTTCCCCAGCAGGATCCTCTATGCAG ACTTCAAGCAGAGATACAAAGTGTTAAACGCAAGTGCCATTCCTGAGGGTCAGTACATCGACAGCAAGAA GGCTTCTGAGAAGCTCCTGGGCTCCATTGACATTGACCACACTCAGTATAAGTTTGGTCACACCAAGGTC TTTTTCAAAGCTGGTCTTCTGGGGCTCCTAGAGGAGATGAGAGATGACAAGTTGGCCCAGCTTATTACCC GGACCCAGGCTATGTGCAGAGGGTTCTTGGCGAGAGTGGAGTACCAGAAGATGGTGGAGAGAAGGGAGTC CATCTTCTGCATCCAGTACAACATCCGCGCCTTCATGAACGTCAAGCACTGGCCCTGGATGAAACTCTTC TTCAAGATCAAGCCCCTGCTGAAGAGCGCAGAGACCGAGAAGGAGATGGCCACCATGAAGGAGGAGTTCC AGAAAACCAAAGATGACCTTGCCAAGTCAGAGGCAAAGAGGAAGGAACTTGAAGAAAAGATGGTGTCCCT CTTGAAAGAAAAAAATGACTTGCAACTTCAAGTTCAGGCTGAAGCTGAAGGCTTGGCCGATGCAGAGGAG CGATGCGACCAGCTGATCAAAACCAAAATCCAGCTGGAGGCCAAAATCAAAGAGGTGACCGAGAGGGCCG AGGATGAGGAGGAGATCAACGCAGAGCTCACGGCCAAGAAGAGGAAGCTGGAGGACGAGTGCTCAGAGCT GAAGAAGGACATCGATGACCTTGAACTGACACTGGCCAAGGTTGAGAAAGAGAAGCATGCCACGGAGAAT AAGGTGAAAAACCTGACAGAGGAGATGGCAGGCCTGGATGAAACCATCGCCAAGCTGACCAAGGAGAAGA AGGCCCTCCAGGAGGCCCACCAGCAGACCCTGGATGACCTGCAGGCAGAGGAGGACAAAGTCAACACCCT GACCAAAGCCAAAATCAAGCTGGAACAGCAAGTGGATGATCTTGAAGGGTCCTTGGAGCAAGAAAAGAAA CTTCGCATGGACCTAGAAAGAGCCAAGAGAAAACTTGAGGGCGACTTGAAGTTAGCCCAGGAGTCCATCA TGGACATTGAAAATGAGAAACAGCAGCTTGATGAAAGGCTCAAAAAGAAAGAGTTTGAAATGAGCAATCT GCAGAGCAAGATTGAGGACGAGCAGGCCATCGGCATTCAGCTGCAGAAGAAGATCAAGGAGTTGCAGGCC CGCATCGAGGAGCTGGAGGAGGAAATCGAGGCAGAGCGGGCCTCCAGGGCCAAAGCAGAGAAGCAGCGCT CTGACCTCTCCCGGGAACTGGAGGAGATCAGCGAGAGGCTGGAAGAAGCCGGCGGGGCCACTTCAGCCCA GATCGAGATGAACAAGAAGAGAGAGGCTGAGTTCCAGAAAATGCGCAGGGACCTGGAGGAGGCCACGCTG CAGCATGAAGCCACAGCAGCCACCCTGAGGAAGAAGCACGCGGACAGCGTGGCTGAGCTCGGGGAGCAGA TCGACAACCTGCAGCGGGTGAAGCAGAAGCTGGAGAAGGAGAAGAGCGAGATGAAGATGGAGATCGATGA TCTTGCCAGTAATGTAGAGACAGTGTCTAAGGCCAAGGGAAACCTCGAGAAGATGTGCCGCACCCTGGAG GACCAGGTGAGTGAGCTGAAGTCAAAGGAGGAGGAACAGCAGCGACTGATCAACGACCTGACAAGCCAGA GAGGACGACTGCAGACCGAATCCGGTGAATTTTCCAGGCAGCTTGATGAGAAGGAAGCGCTGGTATCTCA GTTATCAAGGGGGAAGCAAGCGTTCACTCAACAGATTGAGGAGCTGAAGAGGCAGCTTGAAGAGGAAGTA AAGGCCAAGAACGCGCTGGCCCACGCCCTGCAGTCCTCCCGCCATGACTGTGACCTGCTGCGGGAACAGT ACGAGGAGGAGCAGGAGTCTAAGGCTGAACTGCAGAGGGCGCTGTCCAAGGCCAACAGCGAGGTGGCCCA GTGGAGGACCAAATATGAGACGGATGCCATCCAGCGCACGGAGGAGCTGGAGGAGGCCAAGAAGAAGCTG GCTCAGCGTCTGCAGGCGGCCGAGGAGCACGTAGAAGCCGTGAACGCCAAGTGCGCTTCCCTGGAGAAGA CGAAGCAGCGGCTGCAGAACGAGGTGGAGGACCTCATGCTGGATGTGGAGAGGACCAATGCCGCCTGCGC CGCCCTGGACAAGAAGCAGAGAAACTTCGACAAGATCCTGGCAGAGTGGAAGCAGAAGTATGAGGAAACC CACGCTGAGCTTGAGGCATCCCAGAAGGAGGCCCGCTCCCTGGGCACTGAGCTCTTCAAGATGAAGAATG CCTACGAGGAGTCTCTGGATCAGCTAGAAACCCTGAAGCGAGAGAATAAGAACTTACAGCAGGAGATTTC TGACCTCACGGAACAGATTGCAGAAGGAGGAAAGCGCATCCACGAACTGGAGAAAATTAAGAAACAAGTC GAACAAGAGAAGTGTGAACTTCAGGCTGCTCTAGAAGAAGCAGAGGCATCTCTGGAGCACGAGGAGGGAA AGATCCTGCGCATCCAGCTGGAGCTGAACCAAGTCAAGTCTGAGATCGACAGGAAGCCCGCAATGCAGAA GAGAAAGCCAAGAAAGCCATCACTGATGCCGCCA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG212047 representing BC008538
Red=Cloning site Green=Tags(s) MSSDAEMAVFGEAAPYLRKSEKERIEAQNRPFDAKTSVFVAEPKESFVKGTIQSKDAGKVTVKTEAGATL TVKEDQIFPMNPPKYDKIEDMAMMTHLHEPAVLYNLKERYAAWMIYTYSGLFCVTVNPYKWLPVYNPEVV AAYRGKKRQEAPPHIFSISDNAYQFMLTDRENQSILITGESGAGKTVNTKRVIQYFATIAVTGDKKKEEA TSGKMQGTLEDQIISANPLLEAFGNAKTVRNDNSSRFGKFIRIHFGTTGKLASADIETYLLEKSRVTFQL KAERSYHIFYQITSNKKPELIEMLLITTNPYDYPFVSQGEISVASIDDQEELMATDSAIDILGFTNDEKV SIYKLTGAVMHYGNMKFKQKQREEQAEPDGTEVADKAAYLQGLNSADLLKALCYPRVKVGNEYVTKGQTV EQVTNAVGALAKAMYEKMFLWMVTRINQQLDTKQPRQYFIGVLDIAGFEIFDFNSLEQLCINFTNEKLQQ FFNHHMFVLEQEEYKKEGIEWTFIDFGMDLAACIELIEKPMGIFSILEEECMFPKATDTSFKNKLYEQHL GKSANFQKPKVVKGKAEAHFSLIHYAGTVDYNITGWLDKNKDPLNETVVGLYQKSSVKTLAYLFSGAQTA EAEASSGGAAKKGAKKKGSSFQTVSALFRENLNKLMTNLRSTHPHFVRCIIPNETKTPGAMEHELVLHQL RCNGVLEGIRICRKGFPSRILYADFKQRYKVLNASAIPEGQYIDSKKASEKLLGSIDIDHTQYKFGHTKV FFKAGLLGLLEEMRDDKLAQLITRTQAMCRGFLARVEYQKMVERRESIFCIQYNIRAFMNVKHWPWMKLF FKIKPLLKSAETEKEMATMKEEFQKTKDDLAKSEAKRKELEEKMVSLLKEKNDLQLQVQAEAEGLADAEE RCDQLIKTKIQLEAKIKEVTERAEDEEEINAELTAKKRKLEDECSELKKDIDDLELTLAKVEKEKHATEN KVKNLTEEMAGLDETIAKLTKEKKALQEAHQQTLDDLQAEEDKVNTLTKAKIKLEQQVDDLEGSLEQEKK LRMDLERAKRKLEGDLKLAQESIMDIENEKQQLDERLKKKEFEMSNLQSKIEDEQAIGIQLQKKIKELQA RIEELEEEIEAERASRAKAEKQRSDLSRELEEISERLEEAGGATSAQIEMNKKREAEFQKMRRDLEEATL QHEATAATLRKKHADSVAELGEQIDNLQRVKQKLEKEKSEMKMEIDDLASNVETVSKAKGNLEKMCRTLE DQVSELKSKEEEQQRLINDLTSQRGRLQTESGEFSRQLDEKEALVSQLSRGKQAFTQQIEELKRQLEEEV KAKNALAHALQSSRHDCDLLREQYEEEQESKAELQRALSKANSEVAQWRTKYETDAIQRTEELEEAKKKL AQRLQAAEEHVEAVNAKCASLEKTKQRLQNEVEDLMLDVERTNAACAALDKKQRNFDKILAEWKQKYEET HAELEASQKEARSLGTELFKMKNAYEESLDQLETLKRENKNLQQEISDLTEQIAEGGKRIHELEKIKKQV EQEKCELQAALEEAEASLEHEEGKILRIQLELNQVKSEIDRKPAMQKRKPRKPSLMPP TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC008538 |
| ORF Size | 4794 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC008538.1 |
| RefSeq Size | 5595 bp |
| RefSeq ORF | 4796 bp |
| Locus ID | 17882 |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC224569 | Myh2 (untagged) - Mouse myosin, heavy polypeptide 2, skeletal muscle, adult (cDNA clone MGC:7639 IMAGE:3495412), (10ug) |
CNY 19,190.00 |
|
| MR212047 | Myh2 (Myc-DDK-tagged) - Mouse myosin, heavy polypeptide 2, skeletal muscle, adult (cDNA clone MGC:7639 IMAGE:3495412) |
CNY 15,960.00 |
|
| MR212047L3 | Lenti ORF clone of Myh2 (Myc-DDK-tagged) - Mouse myosin, heavy polypeptide 2, skeletal muscle, adult (cDNA clone MGC:7639 IMAGE:3495412) |
CNY 17,860.00 |
|
| MR212047L4 | Lenti ORF clone of Myh2 (mGFP-tagged) - Mouse myosin, heavy polypeptide 2, skeletal muscle, adult (cDNA clone MGC:7639 IMAGE:3495412) |
CNY 17,860.00 |
