Dnmt1 (NM_010066) Mouse Tagged ORF Clone
CAT#: MG212056
- TrueORF®
Dnmt1 (tGFP-tagged) - Mouse DNA methyltransferase (cytosine-5) 1 (Dnmt1)
ORF Plasmid: DDK
"NM_010066" in other vectors (4)
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CNY 17,770.00
Cited in 1 publication. |
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | Cxxc9; Dnmt; Dnmt1o; m.MmuI; MCMT; Met-1; Met1; MommeD; MommeD2; MTa; MTase |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG212056 representing NM_010066
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCAGCGCGAACAGCTCCAGCCCGAGTGCCTGCGCTTGCCTCCCCGGCAGGCTCGCTCCCGGACCATG TCCGCAGGCGGCTCAAAGACTTGGAAAGAGATGGCTTAACAGAAAAGGAGTGTGTGAGGGAGAAATTAAA CTTACTGCATGAATTCCTGCAAACAGAAATAAAAAGCCAGTTGTGTGACTTGGAAACCAAATTACATAAA GAGGAATTATCTGAGGAAGGCTACCTGGCTAAAGTCAAGTCCCTCTTAAATAAGGATTTGTCCTTGGAGA ACGGAACACACACTCTCACTCAAAAAGCCAACGGTTGTCCCGCCAACGGGAGCCGGCCAACCTGGAGAGC AGAAATGGCAGACTCAAATAGATCCCCAAGATCCAGGCCCAAGCCTCGGGGACCCAGGAGAAGCAAGTCG GACAGTGACACCCTTTCAGTTGAAACTTCACCTAGTTCCGTGGCTACGAGGAGAACCACCAGGCAGACCA CCATCACGGCTCACTTCACGAAGGGCCCCACTAAACGGAAACCCAAGGAAGAGTCGGAAGAGGGGAACTC GGCTGAGTCGGCTGCAGAGGAGAGAGACCAGGATAAGAAACGCAGAGTTGTAGACACAGAGAGTGGTGCT GCAGCTGCTGTGGAGAAACTGGAAGAGGTAACAGCGGGAACCCAGCTGGGTCCGGAAGAGCCATGTGAAC AGGAAGATGACAACAGGAGTCTTCGACGTCACACCAGAGAGCTATCATTGAGGCGGAAATCAAAGGAGGA TCCAGACAGAGAAGCAAGACCGGAAACTCACTTGGACGAGGACGAGGACGGAAAAAAGGATAAAAGAAGT TCCAGACCCAGGAGCCAGCCCAGAGATCCAGCTGCCAAACGGAGACCCAAGGAAGCAGAGCCAGAGCAGG TAGCTCCAGAGACTCCCGAGGACAGAGACGAGGATGAGAGGGAGGAGAAGAGACGAAAAACGACACGTAA AAAACTGGAGTCACACACCGTTCCCGTTCAGAGCAGATCGGAGAGAAAAGCCGCTCAAAGCAAAAGTGTG ATCCCGAAGATCAACTCACCAAAGTGCCCCGAGTGTGGCCAGCACCTAGACGACCCTAACCTGAAGTACC AGCAGCACCCTGAGGATGCTGTGGATGAACCCCAGATGTTGACCAGTGAGAAACTGTCCATCTACGACTC CACCTCGACCTGGTTTGATACTTATGAAGATTCTCCCATGCATAGGTTCACTTCCTTCAGTGTGTACTGC AGTCGCGGGCACCTGTGTCCTGTCGACACCGGTCTCATTGAGAAGAATGTAGAGCTCTACTTTTCTGGGT GTGCCAAAGCAATTCATGACGAGAATCCATCTATGGAAGGTGGTATTAATGGCAAAAACCTCGGGCCAAT CAATCAGTGGTGGCTCAGTGGCTTTGATGGTGGCGAGAAGGTGCTCATTGGCTTCTCCACTGCATTTGCT GAATACATTTTGATGGAGCCCAGCAAAGAGTATGAGCCAATATTTGGGCTGATGCAGGAGAAAATTTACA TCAGCAAGATTGTTGTTGAGTTCCTGCAAAACAATCCTGATGCTGTATATGAAGACCTGATCAATAAGAT TGAGACCACTGTTCCTCCTTCTACCATTAATGTGAACCGGTTCACAGAGGACTCCCTCTTACGCCACGCC CAGTTTGTAGTGAGCCAGGTAGAGAGTTACGACGAAGCCAAGGACGATGATGAGACCCCCATCTTCTTGT CTCCCTGTATGAGAGCCCTGATCCATTTGGCTGGTGTCTCCCTGGGACAGAGGCGAGCAACAAGGCGCGT CATGGGTGCTACCAAGGAGAAGGACAAAGCACCCACGAAAGCCACCACCACCAAGCTGGTCTATCAGATC TTTGACACTTTCTTCTCAGAGCAGATTGAGAAGTATGATAAGGAGGACAAGGAGAATGCCATGAAGCGCC GCCGCTGTGGTGTCTGTGAGGTCTGTCAGCAGCCTGAGTGTGGGAAGTGCAAGGCGTGCAAAGATATGGT GAAGTTTGGTGGCACTGGACGGAGTAAGCAGGCTTGCCTCAAGAGGAGGTGTCCTAACTTGGCGGTGAAG GAGGCAGACGACGATGAAGAGGCTGATGATGATGTGTCAGAGATGCCATCACCCAAAAAGCTGCATCAGG GGAAGAAGAAGAAGCAGAACAAGGACCGCATCTCCTGGCTTGGGCAGCCTATGAAGATTGAAGAGAATAG AACTTACTATCAGAAGGTGAGCATCGATGAGGAGATGCTAGAGGTGGGCGACTGCGTCTCGGTCATTCCA GATGATTCCTCCAAACCACTCTATCTAGCCAGGGTCACAGCTCTGTGGGAAGACAAAAATGGTCAGATGA TGTTCCATGCGCACTGGTTCTGCGCTGGGACAGACACAGTCCTGGGAGCCACCTCCGACCCCCTGGAACT GTTCCTGGTGGGCGAGTGCGAAAACATGCAGCTTTCCTACATCCACAGCAAGGTCAAGGTCATCTACAAA GCCCCTTCTGAAAACTGGGCCATGGAGGGAGGCACAGACCCTGAGACCACACTGCCTGGGGCTGAGGATG GCAAGACTTACTTCTTCCAGCTCTGGTACAACCAGGAGTACGCAAGGTTTGAATCCCCACCCAAGACCCA GCCGACCGAGGACAACAAGCACAAGTTCTGCCTATCTTGTATCCGGCTGGCTGAGCTGAGACAAAAAGAA ATGCCCAAGGTCCTGGAACAAATTGAGGAGGTGGATGGCCGGGTCTACTGCAGTTCCATCACCAAGAATG GTGTTGTCTACCGACTGGGTGACAGTGTGTACCTTCCTCCCGAGGCCTTTACTTTCAACATCAAAGTGGC TAGCCCCGTGAAACGCCCAAAGAAGGATCCTGTGAACGAGACCCTGTACCCTGAGCACTACCGCAAGTAT TCTGACTACATCAAGGGGAGCAACCTGGATGCTCCAGAGCCCTATCGCATCGGTCGGATAAAAGAGATCC ACTGTGGCAAGAAGAAAGGCAAGGTCAACGAGGCAGACATCAAGCTGAGGCTCTACAAGTTCTACAGGCC TGAGAATACCCACAGGTCCTACAACGGATCCTATCACACTGACATCAACATGCTTTACTGGAGCGACGAG GAAGCTGTGGTGAACTTCAGCGACGTGCAGGGCCGCTGTACCGTGGAGTACGGGGAAGACCTACTTGAGA GCATCCAGGATTATTCACAAGGGGGCCCTGACCGCTTCTACTTCCTCGAGGCCTACAATTCAAAGACCAA GAACTTTGAAGACCCACCAAACCATGCCCGCAGCCCTGGGAACAAAGGGAAAGGGAAGGGGAAAGGGAAG GGGAAGGGGAAGCATCAGGTGTCAGAGCCCAAAGAGCCTGAGGCAGCCATCAAACTGCCCAAGCTCCGGA CCCTGGATGTGTTTTCCGGCTGTGGAGGGTTATCGGAAGGATTCCACCAAGCAGGCATCTCGGAAACGCT GTGGGCCATCGAGATGTGGGACCCGGCAGCCCAGGCATTTCGGCTGAACAACCCCGGCACCACAGTGTTC ACAGAGGACTGCAACGTGCTTCTTAAGCTGGTCATGGCTGGGGAGGTGACCAACTCTCTGGGCCAAAGGC TGCCACAGAAGGGCGATGTGGAGATGCTGTGTGGTGGGCCACCCTGCCAGGGCTTCAGTGGCATGAACCG CTTCAACTCCCGCACTTACTCCAAGTTCAAAAACTCCCTAGTGGTCTCCTTCCTCAGCTACTGTGACTAC TACCGGCCTCGGTTCTTCCTTCTGGAGAACGTCAGGAACTTCGTGTCCTACAGACGCTCCATGGTGCTGA AGCTCACACTGCGCTGCCTGGTCCGCATGGGCTACCAGTGCACCTTTGGTGTGCTCCAGGCTGGACAGTA TGGCGTGGCCCAGACACGAAGGAGGGCCATCATCTTGGCTGCAGCCCCAGGAGAAAAGCTGCCTCTGTTC CCAGAGCCTCTGCATGTGTTTGCGCCCCGTGCCTGCCAGCTGAGCGTTGTGGTGGATGACAAGAAGTTTG TTAGCAACATAACGAGGCTGAGCTCGGGGCCCTTCCGAACCATCACCGTGCGAGACACCATGTCTGACCT CCCCGAGATCCAGAATGGAGCCTCGAATTCTGAGATCCCCTACAATGGAGAGCCACTGTCCTGGTTCCAG AGGCAGCTGCGAGGATCACACTACCAGCCCATCCTCAGGGACCATATCTGCAAGGACATGAGCCCACTGG TGGCTGCCCGCATGCGGCACATCCCACTGTTCCCAGGATCAGATTGGCGTGACCTGCCCAACATACAGGT GCGGCTGGGAGATGGCGTCATAGCCCATAAGCTACAGTACACCTTTCATGATGTGAAAAATGGCTACAGC AGCACCGGTGCCCTGCGTGGAGTCTGTTCCTGTGCAGAAGGCAAGGCCTGCGACCCTGAGTCCAGGCAAT TCAGCACCCTCATCCCCTGGTGCCTGCCGCACACTGGGAACCGGCACAACCACTGGGCTGGCCTCTACGG GCGTCTGGAGTGGGATGGCTTCTTCAGCACCACTGTCACCAACCCTGAGCCCATGGGCAAGCAGGGTCGG GTGCTCCACCCGGAGCAGCACCGGGTCGTGAGTGTTCGGGAATGTGCCCGCTCCCAGGGCTTTCCAGATA GCTACCGGTTCTTCGGCAACATCCTGGACAGACACCGGCAGGTGGGTAATGCTGTGCCACCACCCCTGGC CAAAGCCATTGGCCTGGAGATTAAGCTCTGCCTGCTGTCCAGTGCTCGGGAGAGCGCATCAGCTGCAGTT AAAGCAAAAGAGGAGGCTGCTACCAAGGAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG212056 representing NM_010066
Red=Cloning site Green=Tags(s) MPARTAPARVPALASPAGSLPDHVRRRLKDLERDGLTEKECVREKLNLLHEFLQTEIKSQLCDLETKLHK EELSEEGYLAKVKSLLNKDLSLENGTHTLTQKANGCPANGSRPTWRAEMADSNRSPRSRPKPRGPRRSKS DSDTLSVETSPSSVATRRTTRQTTITAHFTKGPTKRKPKEESEEGNSAESAAEERDQDKKRRVVDTESGA AAAVEKLEEVTAGTQLGPEEPCEQEDDNRSLRRHTRELSLRRKSKEDPDREARPETHLDEDEDGKKDKRS SRPRSQPRDPAAKRRPKEAEPEQVAPETPEDRDEDEREEKRRKTTRKKLESHTVPVQSRSERKAAQSKSV IPKINSPKCPECGQHLDDPNLKYQQHPEDAVDEPQMLTSEKLSIYDSTSTWFDTYEDSPMHRFTSFSVYC SRGHLCPVDTGLIEKNVELYFSGCAKAIHDENPSMEGGINGKNLGPINQWWLSGFDGGEKVLIGFSTAFA EYILMEPSKEYEPIFGLMQEKIYISKIVVEFLQNNPDAVYEDLINKIETTVPPSTINVNRFTEDSLLRHA QFVVSQVESYDEAKDDDETPIFLSPCMRALIHLAGVSLGQRRATRRVMGATKEKDKAPTKATTTKLVYQI FDTFFSEQIEKYDKEDKENAMKRRRCGVCEVCQQPECGKCKACKDMVKFGGTGRSKQACLKRRCPNLAVK EADDDEEADDDVSEMPSPKKLHQGKKKKQNKDRISWLGQPMKIEENRTYYQKVSIDEEMLEVGDCVSVIP DDSSKPLYLARVTALWEDKNGQMMFHAHWFCAGTDTVLGATSDPLELFLVGECENMQLSYIHSKVKVIYK APSENWAMEGGTDPETTLPGAEDGKTYFFQLWYNQEYARFESPPKTQPTEDNKHKFCLSCIRLAELRQKE MPKVLEQIEEVDGRVYCSSITKNGVVYRLGDSVYLPPEAFTFNIKVASPVKRPKKDPVNETLYPEHYRKY SDYIKGSNLDAPEPYRIGRIKEIHCGKKKGKVNEADIKLRLYKFYRPENTHRSYNGSYHTDINMLYWSDE EAVVNFSDVQGRCTVEYGEDLLESIQDYSQGGPDRFYFLEAYNSKTKNFEDPPNHARSPGNKGKGKGKGK GKGKHQVSEPKEPEAAIKLPKLRTLDVFSGCGGLSEGFHQAGISETLWAIEMWDPAAQAFRLNNPGTTVF TEDCNVLLKLVMAGEVTNSLGQRLPQKGDVEMLCGGPPCQGFSGMNRFNSRTYSKFKNSLVVSFLSYCDY YRPRFFLLENVRNFVSYRRSMVLKLTLRCLVRMGYQCTFGVLQAGQYGVAQTRRRAIILAAAPGEKLPLF PEPLHVFAPRACQLSVVVDDKKFVSNITRLSSGPFRTITVRDTMSDLPEIQNGASNSEIPYNGEPLSWFQ RQLRGSHYQPILRDHICKDMSPLVAARMRHIPLFPGSDWRDLPNIQVRLGDGVIAHKLQYTFHDVKNGYS STGALRGVCSCAEGKACDPESRQFSTLIPWCLPHTGNRHNHWAGLYGRLEWDGFFSTTVTNPEPMGKQGR VLHPEQHRVVSVRECARSQGFPDSYRFFGNILDRHRQVGNAVPPPLAKAIGLEIKLCLLSSARESASAAV KAKEEAATKD TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_010066 |
ORF Size | 4857 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_010066.1 |
RefSeq Size | 5367 bp |
RefSeq ORF | 4860 bp |
Locus ID | 13433 |
UniProt ID | P13864 |
Gene Summary | This gene encodes a methyltransferase that preferentially methylates cytosines of CpG residues in hemimethylated DNA to generate fully methylated CpG base pairs during DNA replication. This enzyme plays roles in diverse cellular processes including cell cycle regulation, DNA repair, and telomere maintenance. The encoded protein is composed of an N-terminal domain with a nuclear localization sequence and replication fork-targeting domain, a DNA-binding CXXC domain, two bromo-adjacent homology domains, and a C-terminal catalytic domain. Mouse embryonic stem cells mutant for this gene are viable, but when introduced into the germ line, cause a recessive lethal phenotype with mutant embryos displaying stunted growth and developmental defects. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
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Targeting EP4 downstream c-Jun through ERK1/2-mediated reduction of DNMT1 reveals novel mechanism of solamargine-inhibited growth of lung cancer cells
,Chen, Y;Tang, Q;Xiao, Q;Yang, L;Hann, SS;,
J. Cell. Mol. Med.
,PubMed ID 27620163
[Dnmt1]
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC224751 | Dnmt1 (untagged) - Mouse DNA methyltransferase (cytosine-5) 1 (Dnmt1), transcript variant 2, (10ug) |
CNY 20,710.00 |
|
MR212056 | Dnmt1 (Myc-DDK-tagged) - Mouse DNA methyltransferase (cytosine-5) 1 (Dnmt1), transcript variant 2 |
CNY 16,624.00 |
|
MR212056L3 | Lenti ORF clone of Dnmt1 (Myc-DDK-tagged) - Mouse DNA methyltransferase (cytosine-5) 1 (Dnmt1), transcript variant 2 |
CNY 19,024.00 |
|
MR212056L4 | Lenti ORF clone of Dnmt1 (mGFP-tagged) - Mouse DNA methyltransferase (cytosine-5) 1 (Dnmt1), transcript variant 2 |
CNY 19,024.00 |