Jmjd3 (BC075632) Mouse Tagged ORF Clone

CAT#: MG212060

  • TrueORF®

Jmjd3 (tGFP-tagged) - Mouse jumonji domain containing 3 (cDNA clone MGC:91376 IMAGE:30362899)



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Mouse Protein / Lysate Request

CNY 13,300.00


货期*
5周

规格
    • 10 ug

Cited in 1 publication.

Product images

经常一起买 (5)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


Kdm6b Rabbit polyclonal Antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00

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Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms mKIAA0346, RP23-5O23.5, 1700064E03Rik
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG212060 representing BC075632
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCATCGGGCAGTGGACCCTCCAGGGGCCCGCTCTGCACGGGAAGCCTTTGCCCTTGGGGGCTTGAGCT
GTGCTGGGGCTTGGAGCTCCTGCCCACCCCATCCTCCTCCCCGAAGCTCATGGCTGCCCGGAGGCAGATG
CTCTGCCAGCGTTGGGCAGCCCCCACTCTCAGCTCCTTTACCCCCATCTCATGGCAGTAGCTCCGGGCAC
CCTAACAAACCCTATTATGCTCCTGGGACACCCACCCCAAGACCCCTTCACGGGAAGTTGGAATCCCTAC
ATGGCTGTGTCCAGGCATTGCTCCGGGAGCCAGCGCAGCCAGGGTTGTGGGAACAGCTTGGACAGTTGTA
TGAATCAGAGCACGACAGTGAGGAAGCCGTATGCTGCTACCATAGGGCCCTTCGCTATGGAGGAAGCTTC
GCCGAGCTGGGACCCCGGATTGGCCGCTTGCAGCAGGCCCAGCTCTGGAACTTTCATGCCGGTTCCTGTC
AGCACAGAGCCAAGGTCCTGCCTTCCCTGGAGCAAGTCTGGAATTTGCTGCACCTTGAGCACAAACGGAA
CTATGGGGCTAAGCGAGGGGGCCCTCCAGTGAAGAGATCTGCTGAACCCCCCGTGGTCCAGCCTATGCCT
CCTGCAGCCCTCTCAGGCCCCTCAGGAGAGGAGGGCCTTAGCCCTGGAGGCAAGCGCAGGAGAGGCTGCA
GCTCTGAACAGGCTGGCCTTCCCCCAGGTCTGCCACTCCCTCCACCACCCCCACCCCCACCGCCTCCACC
ACCACCACCACCCCCTCCACCACCACCGCTGCCTGGCCTGGCTATTAGCCCCCCATTTCAGCTGACTAAG
CCAGGGCTGTGGAATACCCTGCATGGAGATGCTTGGGGCCCCGAGCGCAAGGGTTCAGCGCCGCCAGAGC
GCCAGGAGCAGCGGCACTCGATGCCTCATTCATATCCATACCCAGCTCCCGCCTACTCCGCTCATCCGCC
CAGCCATCGGCTGGTCCCCAACACACCCCTTGGTCCAGGTCCCCGACCCCCAGGAGCAGAGAGCCATGGC
TGCCTGCCTGCCACCCGTCCCCCCGGAAGTGACCTTAGAGAGAGCAGAGTTCAGAGGTCGCGGATGGACT
CCAGCGTTTCACCAGCAGCATCTACCGCCTGCGTGCCTTACGCCCCTTCCCGGCCCCCTGGCCTCCCCGG
CACCAGCAGCAGCAGCAGCAGCAGCAGTAGCAGTAACAACACTGGTCTTCGGGGTGTGGAGCCAAGCCCA
GGCATTCCTGGCGCTGACCATTACCAAAACCCTGCGCTGGAGATATCCCCTCACCAGGCCCGCCTGGGTC
CCTCCGCACACAGCAGTCGGAAACCATTTTTGACGGCCCCTGCTGCCACGCCCCACTTATCCCTACCCCC
TGGGACCCCATCATCCCCTCCACCCCCATGTCCTCGCCTCTTGCGCCCTCCACCGCCCCCTGCTTGGATG
AAGGGCTCAGCCTGCCGTGCAGCCCGAGAGGATGGAGAGATCTTAGGGGAGCTCTTCTTTGGTGCTGAGG
GACCTCCCCGTCCTCCTCCCCCACCCCTTCCCCACCGTGATGGCTTCTTGGGGCCTCCAAACCCCCGCTT
TTCTGTGGGCACTCAGGATTCGCATAACCCTCCCATTCCCCCAACCACCACCAGCAGCAGCAGCAGCAGC
AACAGCCACAGCAGTAGTCCTACTGGGCCGGTGCCCTTTCCACCACCCTCCTATCTGGCCAGAAGTATAG
ACCCCCTCCCCAGGCCATCCAGCCCAACCTTGAGCCCCCAGGACCCACCTCTTCCACCACTGACTCTTGC
CCTGCCTCCAGCCCCTCCCTCCTCCTGCCACCAAAATACCTCAGGAAGCTTCAGGCGCTCGGAGAGCCCC
CGGCCCAGGGTCTCCTTCCCAAAGACCCCCGAGGTGGGGCAGGGGCCACCCCCAGGCCCTGTGAGTAAAG
CCCCCCAGCCTGTGCCACCTGGGGTTGGAGAGCTGCCTGCCCGAGGCCCGAGGCTCTTTGATTTCCCACC
CACTCCGCTGGAGGACCAGTTTGAAGAGCCAGCCGAATTCAAGATCCTACCTGATGGGCTGGCAAACATC
ATGAAGATGCTGGATGAATCCATTCGGAAGGAGGAGGAGCAGCAGCAGCAGCAGGAGGCAGGCGTGGCTC
CCCCACCCCCACTCAAAGAGCCCTTTGCATCTCTACAGCCTCCATTTCCCAGTGACACAGCCCCAGCCAC
CACCACTGCTGCCCCCACCACCGCCACCACCACCACAACCACCACCACCACCACCACCCAAGAAGAGGAG
AAGAAGCCACCACCAGCCCTACCACCACCACCGCCTCTAGCCAAGTTTCCTCCACCTCCCCAGCCACAGC
CCCCACCACCTCCACCAGCCAGCCCAGCCAGCCTGCTCAAATCGTTGGCCTCTGTTCTTGAGGGACAAAA
GTACTGTTACCGGGGGACTGGAGCAGCCGTCTCAACCAGGCCCGGGTCCGTGCCCGCCACTCAGTATTCC
CCTAGTCCTGCATCAGGTGCTACCGCCCCACCACCCACTTCAGTGGCCCCTAGTGCCCAGGGCTCCCCCA
AGCCCTCGGTTTCCTCGTCATCTCAGTTCTCTACCTCAGGCGGGCCTTGGGCCCGGGAGCACAGGGCGGG
TGAAGAGCCAGCACCAGGCCCCGTGACCCCTGCCCAGTTGCCCCCACCTCTGCCGCTGCCCCCTGCTCGT
TCTGAGTCTGAGGTGCTAGAAGAAATCAGTCGGGCTTGTGAGACCCTTGTAGAGCGGGTGGGCCGGAGTG
CCATCAACCCAGTGGACACGGCAGACCCAGTGGACAGTGGGACTGAGCCACAGCCGCCGCCTGCGCAGGC
CAAGGAGGAGAGTGGGGGGGTGGCGGTAGCAGCAGCAGGTCCAGGTAGTGGCAAGCGTCGTCAAAAGGAG
CATCGGCGGCACAGGCGGGCCTGTAGGGACAGTGTGGGTCGACGACCCCGCGAGGGGAGGGCCAAGGCCA
AGGCCAAGGCTCCCAAAGAAAAAAGCCGAAGGGTGCTGGGGAACCTCGACTTGCAGAGTGAGGAGATCCA
GGGCCGGGAGAAGGCCCGGCCCGATGTCGGTGGGGTTTCCAAAGTCAAGACACCCACAGCTCCAGCACCC
CCGCCTGCTCCTGCACCCGCTGCTCAGCCAACACCCCCATCAGCTCCTGTCCCTGGGAAGAAGACTCGTG
AGGAGGCTCCGGGGCCTCCAGGTGTGAGCCGGGCAGATATGCTGAAGCTCCGGTCACTTAGTGAGGGGCC
TCCCAAGGAGCTGAAGATCAGGCTCATCAAGGTGGAAAGTGGGGACAAGGAGACCTTTATCGCCTCTGAG
GTGGAAGAGCGGCGGCTGCGCATGGCAGACCTCACCATCAGCCACTGTGCCGCCGATGTCATGCGTGCCA
GCAAGAATGCCAAGGTGAAAGGGAAATTCCGAGAGTCCTACCTGTCCCCTGCCCAGTCTGTGAAACCCAA
GATCAACACTGAGGAGAAGCTGCCCCGGGAAAAACTCAACCCCCCTACCCCCAGCATCTATTTGGAGAGC
AAACGAGATGCCTTCTCGCCGGTCCTGCTACAGTTCTGTACAGACCCCCGGAACCCCATCACCGTCATCA
GGGGCCTGGCTGGTTCACTTCGGCTCAACTTAGGCCTTTTCTCCACCAAGACTCTGGTGGAGGCGAGCGG
TGAACATACGGTGGAGGTCCGTACCCAAGTACAGCAGCCCTCAGACGAGAACTGGGACCTGACAGGTACC
AGACAAATCTGGCCCTGTGAGAGCTCCCGTTCCCACACCACCATCGCTAAATACGCACAGTACCAGGCCT
CGTCCTTCCAGGAGTCACTGCAGGAGGAGAGGGAGAGTGAGGATGAGGAATCCGAGGAACCAGACAGCAC
TACAGGAACCTCTCCCAGCAGTGCACCGGACCCCAAGAACCATCACATCATCAAGTTTGGCACTAACATC
GACCTGTCTGATGCCAAGAGGTGGAAGCCACAGCTACAGGAGCTGCTGAAACTGCCCGCCTTCATGCGGG
TAACATCCACAGGCAACATGCTCAGCCACGTGGGCCACACCATCCTGGGCATGAACACCGTGCAGCTATA
CATGAAGGTCCCTGGCAGCCGAACGCCAGGCCACCAAGAGAATAACAATTTCTGCTCAGTCAACATCAAC
ATTGGCCCTGGGGACTGCGAGTGGTTCGCGGTACATGAGCACTATTGGGAGACCATCAGCGCCTTCTGCG
ACCGGCATGGTGTGGACTACTTGACTGGTTCCTGGTGGCCAATCTTGGATGACCTCTATGCGTCCAATAT
TCCTGTTTACCGCTTCGTGCAGCGCCCTGGAGACCTTGTGTGGATTAATGCAGGGACTGTACATTGGGTG
CAGGCTACCGGCTGGTGCAACAACATTGCCTGGAACGTGGGGCCCCTCACCGCCTATCAGTACCAGCTGG
CCCTGGAGCGATATGAGTGGAACGAGGTGAAGAACGTCAAGTCCATTGTGCCCATGATTCATGTGTCCTG
GAACGTCGCTCGAACGGTCAAGATCAGCGATCCTGACTTGTTCAAGATGATCAAGTTCTGCCTCCTGCAG
TCAATGAAGCACTGTCAGGTACAGCGGGAGAGCCTGGTGCGGGCAGGGAAGAAGATCGCTTACCAAGGCC
GTGTCAAAGACGAGCCTGCCTACTACTGCAACGAATGCGACGTGGAGGTGTTCAACATCCTGTTCGTTAC
AAGTGAGAATGGCAGCCGAAACACGTACCTGGTGCACTGCGAGGGCTGTGCGCGCCGTCGCAGCGCGGGC
CTACAGGGCGTGGTGGTGCTAGAGCAGTACCGCACGGAGGAGCTGGCGCAGGCCTACGATGCCTTCACAC
TGGCTCCCGCCAGCACGTCTCGA


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG212060 representing BC075632
Red=Cloning site Green=Tags(s)

MHRAVDPPGARSAREAFALGGLSCAGAWSSCPPHPPPRSSWLPGGRCSASVGQPPLSAPLPPSHGSSSGH
PNKPYYAPGTPTPRPLHGKLESLHGCVQALLREPAQPGLWEQLGQLYESEHDSEEAVCCYHRALRYGGSF
AELGPRIGRLQQAQLWNFHAGSCQHRAKVLPSLEQVWNLLHLEHKRNYGAKRGGPPVKRSAEPPVVQPMP
PAALSGPSGEEGLSPGGKRRRGCSSEQAGLPPGLPLPPPPPPPPPPPPPPPPPPPPLPGLAISPPFQLTK
PGLWNTLHGDAWGPERKGSAPPERQEQRHSMPHSYPYPAPAYSAHPPSHRLVPNTPLGPGPRPPGAESHG
CLPATRPPGSDLRESRVQRSRMDSSVSPAASTACVPYAPSRPPGLPGTSSSSSSSSSSNNTGLRGVEPSP
GIPGADHYQNPALEISPHQARLGPSAHSSRKPFLTAPAATPHLSLPPGTPSSPPPPCPRLLRPPPPPAWM
KGSACRAAREDGEILGELFFGAEGPPRPPPPPLPHRDGFLGPPNPRFSVGTQDSHNPPIPPTTTSSSSSS
NSHSSSPTGPVPFPPPSYLARSIDPLPRPSSPTLSPQDPPLPPLTLALPPAPPSSCHQNTSGSFRRSESP
RPRVSFPKTPEVGQGPPPGPVSKAPQPVPPGVGELPARGPRLFDFPPTPLEDQFEEPAEFKILPDGLANI
MKMLDESIRKEEEQQQQQEAGVAPPPPLKEPFASLQPPFPSDTAPATTTAAPTTATTTTTTTTTTTQEEE
KKPPPALPPPPPLAKFPPPPQPQPPPPPPASPASLLKSLASVLEGQKYCYRGTGAAVSTRPGSVPATQYS
PSPASGATAPPPTSVAPSAQGSPKPSVSSSSQFSTSGGPWAREHRAGEEPAPGPVTPAQLPPPLPLPPAR
SESEVLEEISRACETLVERVGRSAINPVDTADPVDSGTEPQPPPAQAKEESGGVAVAAAGPGSGKRRQKE
HRRHRRACRDSVGRRPREGRAKAKAKAPKEKSRRVLGNLDLQSEEIQGREKARPDVGGVSKVKTPTAPAP
PPAPAPAAQPTPPSAPVPGKKTREEAPGPPGVSRADMLKLRSLSEGPPKELKIRLIKVESGDKETFIASE
VEERRLRMADLTISHCAADVMRASKNAKVKGKFRESYLSPAQSVKPKINTEEKLPREKLNPPTPSIYLES
KRDAFSPVLLQFCTDPRNPITVIRGLAGSLRLNLGLFSTKTLVEASGEHTVEVRTQVQQPSDENWDLTGT
RQIWPCESSRSHTTIAKYAQYQASSFQESLQEERESEDEESEEPDSTTGTSPSSAPDPKNHHIIKFGTNI
DLSDAKRWKPQLQELLKLPAFMRVTSTGNMLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNIN
IGPGDCEWFAVHEHYWETISAFCDRHGVDYLTGSWWPILDDLYASNIPVYRFVQRPGDLVWINAGTVHWV
QATGWCNNIAWNVGPLTAYQYQLALERYEWNEVKNVKSIVPMIHVSWNVARTVKISDPDLFKMIKFCLLQ
SMKHCQVQRESLVRAGKKIAYQGRVKDEPAYYCNECDVEVFNILFVTSENGSRNTYLVHCEGCARRRSAG
LQGVVVLEQYRTEELAQAYDAFTLAPASTSR

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC075632
ORF Size 4925 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC075632, AAH75632
RefSeq Size 5680 bp
RefSeq ORF 4925 bp
Locus ID 216850
Gene Summary Histone demethylase that specifically demethylates 'Lys-27' of histone H3, thereby playing a central role in histone code. Demethylates trimethylated and dimethylated H3 'Lys-27'. Plays a central role in regulation of posterior development, by regulating HOX gene expression. Involved in inflammatory response by participating in macrophage differentiation in case of inflammation by regulating gene expression and macrophage differentiation (PubMed:17825402). Plays a demethylase-independent role in chromatin remodeling to regulate T-box family member-dependent gene expression by acting as a link between T-box factors and the SMARCA4-containing SWI/SNF remodeling complex (PubMed:21095589).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (1)

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