Col4a5 (BC082788) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG212066 representing BC082788 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAAGTGCGTGGAGTGTGCCTGGCTGCCGGCTGGTTCTTACTGGCCCTGTGTCTGTGGGGGCAGCCCG CAGAGGCTGCGGCCTGCCATGGGTGTTCTTCTGGATCAAAATGTGACTGCAGTGGTATAAAAGGAGAAAA GGGAGAACGGGGGTTTCCAGGTTTAGAAGGTCATCCAGGTTTACCAGGATTTCCAGGTCCAGAAGGCCCT CCAGGACCTCGGGGACAAAAGGGTGATGATGGAATTCCAGGGCCACCAGGACCAAAAGGAATCAGAGGTC CTCCTGGACTTCCTGGATTTCCAGGGACACCAGGTCTTCCTGGGATGCCAGGCCATGATGGGGCTCCAGG ACCTCAAGGTATCCCTGGATGCAATGGAACCAAGGGAGAACGTGGATTTCCAGGCAGTCCGGGCTTTCCT GGTTTACAAGGTCCTCCAGGACCTCCTGGGATCCCAGGTATGAAGGGGGAACCAGGCAGTATAATCATGT CACCACTGCCAGGACCAAAGGGTAATCAAGGATACCCAGGTCCTCCTGGAATACAAGGCCCACCTGGTCC CAAGGGTATATCAGGGCCAAGTGGTCCTCCAGGACCACCAGGTTTAATGGGACCTCCTGGCCCACCAGGC CCACCGGGACCAAAGGGGAATATGGGCTTAAATTTCCAGGGACCCAAAGGTGAAAAAGGCGAACAAGGTC TTCAGGGCCCACCTGGTCCACCTGGGCAGATCAGCGAACAGAAAAGACCAATTGACATAGAATTTCAGAA AGGAGATCAGGGAGTTCCTGGTGATAGAGGGCCTCCTGGACCTCCAGGAATACCTGGTCCTCCCGGTCCT CCTGGTGGTGTGAAAGGTGAAAAGGGTGAGCCAGGAGAGGCAGGCAAAAGAGGTAAACCAGGCAAAGATG GAGAGACTGGCCAACCAGGAATCCCAGGTTTGCCTGGTGATCCTGGTTACCCTGGAGAACCAGGAAGAGA TGGTGAAAAGGGTCAAAAGGGTGATATTGGCCCACCTGGACCTCCCGGACTTGTAATTCCTGCACCTGGA ACTGGTGTGACTATGGGAGAAAAAGGAAATATCGGGTTACCTGGTTTGCCTGGAGAAAAGGGAGAGCGAG GATTTCCTGGAATACAAGGTCCACCTGGCTTTCCTGGACCTCCAGGAACAGCAGTTGTGGGTCCCCCTGG TCCTCCTGGATATCCTGGTGAAAGGGGCCAGAAAGGTGATGAAGGTCCCCCTGGAATTTGTATTCCTGGA TCTCCTGGACTTGATGGACAGCCTGGGGCTCCTGGCCTTCCAGGACCTCCTGGCCCCCCTGGCCCCCAGT TGCCATCCAGAGATGAAATCTGTAAAGCAGGCCCTCCTGGGCCTCCAGGACCTCCAGGTGATAAAGGACT CCAAGGAGAGCGAGGAGTAAAAGGTGACAAAGGTGATACTTGCTTCAACTGTATTGGAACTGGCATTTCA GGGCCTCCAGGCCAACCTGGTTTACCAGGTCTCCCAGGTCCTCCAGGATCTCTTGGAATCCCTGGAGAGA AGGGGGACAAAGGACAAGCTGGGATAACTGGTCCGAAAGGATTGCCAGGCATACCTGGACCTCCAGGTGC TCCAGGCTTTCCAGGGTCTAAGGGTGACCCTGGGGATGTCCTCACTCTTCCAGGAATGAAAGGTGACAAA GGAGAGTTGGGTTTCCCTGGAGCTCCAGGGCTTCCTGGTCTACCTGGTAGTCCTGGAAAGGATGGTTTGC CAGGACTCCCTGGCCCCAAAGGAGAGCCTGGTGGAATTACTTTTAAGGGTGAAAGAGGTCCTCCTGGGAG CCCAGGTTTACCAGGTCTCCCAGGAAATATGGGACCTACAGGCCCAGTTGGTTTTGGGCCTCCAGGCCCC ATAGGCGAAAAAGGCATACAAGGTGTGGCAGGAAATCCAGGCCAGCCGGGATTACCAGGTCCTAAAGGAG ATCCAGGCCAGACCATAACCCAGCCAGGAAAGCCTGGTTTGCCAGGTAGTCCAGGCAGAGATGGTGAAGT GGGTCTTCCAGGTGATCCTGGACTTCCAGGCCAACCAGGCCTGCCAGGAATACCTGGTAGCAAAGGAGAA CCAGGTATCCCTGGGATTGGGCCTCCTGGACCACCTGGTCCTAAAGGTTTTCCAGGAATTCCGGGACCTC CAGGAGCTCCTGGGGCCCCTGGAAGAATGGGTCCAGATGGGCCTCCTGGGCCACCAGGCTTCCCAGGACC AAAGGGAGAGCCAGGATTTGGGTTACCTGGGCCACCTGGGCCACCAGGATTCCCAGGTTTCAAAGGAACA CCTGGTCCAAAAGGTGATCGTGGTTTCCCAGGACCTTCAGGTCCTCCAGGACGCACTGGCTTGGATGGGC TACCTGGACCAAAAGGTGATGTTGGACCAAATGGACAACCTGGCCCAGTAGGGCCTCCAGGGCTGCCAGG AATTGGTCTTCAGGGACCACCAGGACCACCAGGGACTCCAGGGCCAATAGGCCAACCTGGCTTACATGGG ATTCCAGGAGAGAAGGGGGACCCAGGACCTCCTGGGTTTGATGTTCCAGGACTCCCTGGAGAGAGAGGTA GTCCAGGGCTCCCTGGAGCCCCTGGTCTTATAGGACCTCCAGGATCACCTGGTGTCCCTGGAAAAGCAGG TGTCCCTGGATTTCCAGGTGTCAAAGGCGAAATGGGTATGATGGGACCTCCAGGCCCACCTGGACCTTTG GGAATTCCTGGCAGGAGTGGTGCTCCTGGTCTTAAAGGTGATGATGGCATGCAAGGTCAGCCAGGTCTCC CTGGCCCTGCAGGAGAAAAGGGCGGTAAGGGAGAGCCTGGCCTTCCAGGTCCTCCTGGACCAATGCATCC AGATTTCCTGGGATCGAAAGGAGAAAAGGGTGAGCCCGGCTTACCAGGTATTCCTGGACTTTCAGGACCA AAAGGCTACCAGGGATTACCTGGAGACCCAGGGCAACCTGGACTGAGTGGACAACCAGGATCACCAGGAC TCCCAGGTCCCAAGGGTAATCCTGGTCTCCCTGGTCCACCAGGACTTACAGGACCTCCTGGACCAAAAGG AAACATTGGTGACATGGGTTTTCCTGGCCCTCAAGGTGTAGATGGGCCCCCTGGACCTCCTGGATTTCCT GGACAACCTGGCTCTCCTGGGTTACCTGGCCAGAAAGGAAGTAAAGGAGAGCCTGGGGTTTCAGGCATTG GACTTCCAGGTCTTCCTGGCCCAAAGGGTGAACCTGGTCTGCCTGGATATCCAGGAAACCCAGGTATCAA AGGGTCGGTGGGAGAGACTGGTCTTCCTGGATTACCAGGAAACCCTGGAGCAAAAGGTCAACCGGGCCTG CCTGGATTCCCAGGAACACCAGGACTTCCTGGACCAAAAGGTATGAATGGTCCTCCTGGTAACCCTGGCT TTCCCGGAGAACCTGGTCCTGTAGGTGCCGGAGGGCGTCCTGGACCACCAGGACCTCCTGGTGAAAAAGG CAAGCCAGGTCAAGATGGCATTCCTGGGCCAGCAGGACAAAAGGGAGAACCAGGTCAACCAGGCTTTGGA ATCCCAGGACCTCCTGGACTCCCAGGACTTTCTGGACAAAAGGGTGATGGAGGATCACCTGGACTTCCAG GAAATCCTGGCCTTCCAGGTTCAAAGGGTGAGCCAGGCTTTCAGGGATTCCCTGGTATGCCAGGTCCCCC AGGTCCTCCTGGTTCTCCTGGTCAAGCTTTGGAAGGTCCCAAAGGAAACCCTGGGCCTCAAGGCCCTCCT GGGAGACCAGGTCCTCCAGGTTTTCAAGGTCCACCAGGTACAGAAGGTCCTCGTGGACCCCCTGGAAATG GAGGTATCAAAGGAGAGAGGGGAAATCCAGGCCCACCTGGGCAGCCAGGCTTACCTGGTTTGAAAGGAGA TCAAGGACCACCAGGACTCCCGGGTAACCCTGGCCGGCCAGGTCTTAATGGAATGAAAGGAGATCCTGGT CTCCCTGGTGTTCCAGGATTCCCAGGAATGAAAGGACCCATTGGAGTACCAGGCTCCACTGGCCCTGATG GGGAACCAGGTCTTACTGGGCCCCCAGGTCCTCCTGGGTTGCCTGGTCCTTCAGGACAGAGTATTGTAAT CAAAGGAGATCCTGGACCTCCAGGGATTCCTGGACAACCTGGATTAAAAGGTCTACCAGGACTTCCAGGC CCTCAAGGCCTACCAGGTCCAATTGGCCCTCCAGGAGATCCAGGACGCAATGGACTCCCAGGCTTTGATG GTGCAGGAGGGCGCAAAGGAGACCCAGGGCTGCCAGGACAGCCAGGTGCCCGTGGATTGGATGGTCCCCC AGGACCAGATGGATTGCAAGGACCTCCAGGACCCCCTGGAACCACCTCTGTTGCCCATGGATTCCTCATC ACACGTCACAGTCAAACAACAGAAGCACCACAATGCCCACGGGGAACAGTTCATATCTATGAAGGCTTTT CTCTCCTGTATGTACAAGGAAATAAAAGAGCCCATGGTCAAGACTTGGGTACGGCTGGCAGCTGCCTTCG TCGCTTCAGTACCATGCCTTTCATGTTCTGCAACATTAACAACGTTTGTAACTTTGCTTCAAGAAATGAC TATTCTTACTGGCTTTCCACCCCAGAGCCCATGCCAATGAACATGGAACCCCTGAAGGGACAGAGCATCC AGCCATTCATTAGTCGATGTGCAGTGTGTGAGGCGCCAGCTGTGGTGATCGCTGTTCACAGCCAGACTAT TCAGATTCCACACTGTCCTCAGGGGTGGGACTCTCTGTGGATTGGCTATTCCTTCATGATGCATACAAGT GCAGGAGCAGAAGGCTCTGGCCAAGCCCTAGCCTCTCCTGGCTCCTGTTTGGAAGAATTTCGTTCCGCTC CCTTCATCGAATGTCATGGGCGAGGGACATGCAATTACTATGCCAATTCGTACAGCTTTTGGCTGGCCAC TGTTGATATGTCAGACATGTTCAACAAACCTCAGTCAGAAACCCTGAAAGCAGGAGACTTGAGGACGCGT ATTAGCCGATGTCAAGTATGCATGAAGAGGACA AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG212066 representing BC082788 Red=Cloning site Green=Tags(s) MQVRGVCLAAGWFLLALCLWGQPAEAAACHGCSSGSKCDCSGIKGEKGERGFPGLEGHPGLPGFPGPEGP PGPRGQKGDDGIPGPPGPKGIRGPPGLPGFPGTPGLPGMPGHDGAPGPQGIPGCNGTKGERGFPGSPGFP GLQGPPGPPGIPGMKGEPGSIIMSPLPGPKGNQGYPGPPGIQGPPGPKGISGPSGPPGPPGLMGPPGPPG PPGPKGNMGLNFQGPKGEKGEQGLQGPPGPPGQISEQKRPIDIEFQKGDQGVPGDRGPPGPPGIPGPPGP PGGVKGEKGEPGEAGKRGKPGKDGETGQPGIPGLPGDPGYPGEPGRDGEKGQKGDIGPPGPPGLVIPAPG TGVTMGEKGNIGLPGLPGEKGERGFPGIQGPPGFPGPPGTAVVGPPGPPGYPGERGQKGDEGPPGICIPG SPGLDGQPGAPGLPGPPGPPGPQLPSRDEICKAGPPGPPGPPGDKGLQGERGVKGDKGDTCFNCIGTGIS GPPGQPGLPGLPGPPGSLGIPGEKGDKGQAGITGPKGLPGIPGPPGAPGFPGSKGDPGDVLTLPGMKGDK GELGFPGAPGLPGLPGSPGKDGLPGLPGPKGEPGGITFKGERGPPGSPGLPGLPGNMGPTGPVGFGPPGP IGEKGIQGVAGNPGQPGLPGPKGDPGQTITQPGKPGLPGSPGRDGEVGLPGDPGLPGQPGLPGIPGSKGE PGIPGIGPPGPPGPKGFPGIPGPPGAPGAPGRMGPDGPPGPPGFPGPKGEPGFGLPGPPGPPGFPGFKGT PGPKGDRGFPGPSGPPGRTGLDGLPGPKGDVGPNGQPGPVGPPGLPGIGLQGPPGPPGTPGPIGQPGLHG IPGEKGDPGPPGFDVPGLPGERGSPGLPGAPGLIGPPGSPGVPGKAGVPGFPGVKGEMGMMGPPGPPGPL GIPGRSGAPGLKGDDGMQGQPGLPGPAGEKGGKGEPGLPGPPGPMHPDFLGSKGEKGEPGLPGIPGLSGP KGYQGLPGDPGQPGLSGQPGSPGLPGPKGNPGLPGPPGLTGPPGPKGNIGDMGFPGPQGVDGPPGPPGFP GQPGSPGLPGQKGSKGEPGVSGIGLPGLPGPKGEPGLPGYPGNPGIKGSVGETGLPGLPGNPGAKGQPGL PGFPGTPGLPGPKGMNGPPGNPGFPGEPGPVGAGGRPGPPGPPGEKGKPGQDGIPGPAGQKGEPGQPGFG IPGPPGLPGLSGQKGDGGSPGLPGNPGLPGSKGEPGFQGFPGMPGPPGPPGSPGQALEGPKGNPGPQGPP GRPGPPGFQGPPGTEGPRGPPGNGGIKGERGNPGPPGQPGLPGLKGDQGPPGLPGNPGRPGLNGMKGDPG LPGVPGFPGMKGPIGVPGSTGPDGEPGLTGPPGPPGLPGPSGQSIVIKGDPGPPGIPGQPGLKGLPGLPG PQGLPGPIGPPGDPGRNGLPGFDGAGGRKGDPGLPGQPGARGLDGPPGPDGLQGPPGPPGTTSVAHGFLI TRHSQTTEAPQCPRGTVHIYEGFSLLYVQGNKRAHGQDLGTAGSCLRRFSTMPFMFCNINNVCNFASRND YSYWLSTPEPMPMNMEPLKGQSIQPFISRCAVCEAPAVVIAVHSQTIQIPHCPQGWDSLWIGYSFMMHTS AGAEGSGQALASPGSCLEEFRSAPFIECHGRGTCNYYANSYSFWLATVDMSDMFNKPQSETLKAGDLRTR ISRCQVCMKRT SGPTRTRRLE - GFP Tag - V
Restriction Sites	SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map
ACCN	BC082788
ORF Size	5075 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC082788, AAH82788
RefSeq Size	5710 bp
RefSeq ORF	5075 bp
Locus ID	12830

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