Bcor (BC058656) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	2900008C10Rik; 5830466J11Rik; 8430401K06Rik; BcorR; D930024N20Rik; mKIAA1575
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG212070 representing BC058656 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTTTCTGCAACCCCTCTGTATGGGAACGTTCACAGCTGGATGAACAGCGAGAGGGTCCGCATGTGTG GAACAAGTGAAGACAGGAAAATTCCTGTGAATGATGGCGACGCTTCAAAAGCCAGGCTGGAGCTGAGGGA GGAGACTCCCCTGAGTCACAGCGTGGTGGATACCAGTGGAGCCCATCGGATCGATGGCCTGGCAGCACTG AGCATGGACCGCACAGGCCTCATCCGGGAAGGGCTCCGGGTCCCTGGAAACATCGTTTATTCTGGCTTGT GTGGATTGGGCTCAGAAAAAGGTCGGGAGGCTACCCCAAGCTCTCTCAGTGGCCTTGGGTTTTCTTCGGA AAGAAATCCAGAGATGCAATTCAAACCGAATACTCCCGAGACAGTGGAGGCTTCTGCTGTCTCTGGAAAA CCCCCAAATGGCTTCAGTGCTATATACAAAACACCGCCTGGGATACAAAAGAGCGCTGTAGCTACAGCGG AAAGCTTGGGTTTGGATAGGCCTGCCAGTGACAAACAGAGCCCTCTCAACATCAATGGTGCTAGTTACCT GCGGCTGCCCTGGGTCAACCCTTACATGGAGGGGGCCACTCCAGCCATATACCCTTTCCTCGACTCGCCA AATAAGTATTCACTGAATATGTACAAGGCCTTGCTACCTCAGCAGTCCTACGGCTTGGCCCAGCCACTGT ATTCTCCTGTCTGCACCAGTGGGGAACGCTTCCTCTACCTGCCACCCCCACACTATGTCAACCCTCACAT CCCCTCCTCCTTGGCTTCACCCATGAGGCTCTCCACACCTTCAGCCTCTGCGGCCATTCCACCTCTCGTC CACTGCTCAGACAAAAGCCTCCCATGGAAGATGGGCGTCAACCCTGGGAACCCAGTAGATTCCCACAGTT ACCCCCACATCCAAAACAGTAAACAGCCCCGGGTGACCTCTGCCAAGGCAGTCAATAGTGGCCTGCCAGG GGACACAGCTCTCCTGTTGCCTCCTTCACCTCGGCCCTCAGCCCGGGTCCACCTTCCAACTCAGCCCGCT GCAGAGACCTACTCCGAGTTCCACAAGCACTATCCCAGGATCTCCACCTCTCCCTCAGTCACCCTGACGA AGCCATACATGACAGCCAATAGCGAGTTCTCTACATCCAGGCTGTCCAATGGCAAGTACCCCAAGGCTCT AGATGGGGGCGACTGTGCTCAATCCATGCCTGGGCACACCCGGAAGACAACGGTTCAAGACAGAAAAGAT GGGGGCTCACCACCTCTGTTGGAAAAGCAGACCGTTACCAAAGATGTCACAGACAAACCACTTGACCTGT CTTCTAAAGTGGTGGATGCGGACGCTTCCAAAGGTGACCACATGAAAAAGATGGCTCCCACAGTCCTGGT TCACAGTCGGGCTGCAAGTGGCTTAGTGCTCTCTGGAAGTGAGATTCCGAAAGAAACACTATCTCCTCCA GGAAATGGATGTTCTATCTATAGATCAGAGATCATCAGCACTGCTCCCTCATCCTGGGTGGTGCCAGGGC CAAGTCCTAATGAAGAGAACAATGGCAAAAGTCTGTCACTGAAAAACAAGGCTTTGGACTGGGCAATACC GCAACAGCGGAGTTCCTCGTGTCCCCGCATGGGTGGCACAGATGCCGTGGTCACTAATGTTTCAGGGTCC GTGTCCAGCTCAGGACGCCCAGCCTCTGCATCACCAGCCCCCAACGCCAACGCCAATGCAGATGGCACCA AGACCAGCAGGAGCTCGGTGGATACCACGCCATCAGTCATCCAGCATGTGGGCCAGCCCTCATCCACGCC TGCCAAGCACGGTGGCAGCACCAGCAGCAAGGGAGCCAAAGCCAACCCAGAACCGAGCTTCAAAGCGAGT GAGAATGGCCTCCCGCCAACCTCAATATTTCTATCTCCAAATGAAGCATTCAGGTCCCCAGCAATTCCCT ACCCTAGGAGTTACCTCCCTTACGCAGCACCAGAAGGCATCGCTCTAAGTCCCCTCTCTTTACATGGCAA AGGACCTGTGTATCCTCACCCAGTTTTGTTACCCAATGGCAGTCTGTTTCCTGGGCACCTTGCCCCAAAG CCTGGCCTGCCTTACGGGCTGCATACGAGCAGGCCAGAATTTGTGACCTACCAAGATGCCCTGGGCCTGG GCATGGTCCATCCCATGTTGATCCCACACACGCCCATTGAGATCACAAAAGAGGAGAAGCCGGAGAGACG CTCCCGCTCCCATGAGAGAGCCCGTTATGAGGACCCAACCCTCCGTAGCCGGTTTTCTGAGATGTTGGAA GCTAGCAGCACCAAACTGCATCCAGAAGTCCCCACTGACAAGAACCTGAAGCCAAACTCTAGCTGGAATC AAGGGAAGACTGGGGTCAAAAGCGACAAACTGGTCTACGTAGACCTTCTCCGAGAAGAAGCAGACACTAA AACTGATGCAGGGGCACCCAAAGCAGGCCTTGTAGCCGAGAATGTAGGTCAGGACACCGAGGCCACCAAG CCCTCAGCTGACCCTGTGATCCAACAACGCCGTGAATTCATCTCCCTCAGAGAGGAGCTGGGCCGAATCA CTGACTTCCATGAATCTTTCACTTTCAAACAGGCCTCAAGCCAGCCAGTGTTCAGCTTAGGCAAGGATAG TGGTGCAGCAGGAACCAACAAAGAGAACCTGGGTGTGCAGGTAGCAACTCCATTCTTGGAGACAGCTCTG GGCAGTGAGGGCCCTGCTGTGACTTTTGGTAAAACCCAAGAGGATCCCAAACCATTTTGTGTGGGCGGTG CCCCTCCAAACATGGATGTCACCCCCGCCTATACCAAAGAAGGAACTGATGAGGCCGAATCCAACGATGG CAAAGTTCTGAAACCCAAGCCATCCAAGCTGGCAAAGAGAATTGCTAACTCGGCAGGTTATGTGGGCGAC CGATTCAAGTGTGTCACCACCGAACTGTATGCAGATTCCAGTCAGCTCAGCCGAGAGCAGCGGGCATTGC AGATGGAAGGATTACAAGAGGACAGTATTTTATGTCTACCCGCTGCTTACTGTGAGCGTGCAATGATGCG CTTCTCAGAGTTGGAGATGAAAGAGAGAGAAGGTAGCCACCCAGCAACCAAAGACTCCGAGGTGTGCAAA TTCAGCCCAGCCGACTGGGAAAGGTTGAAAGGAAATCAGGAAAAGAAGCCAAAGTCAGTCACCCTGGAAG AGGCCATTGCCGACCAGAATGACAGTGAGAGATGTGAGTATAGCACTGGAAACAAACATGATCTCTTTGA AGCCCCAGAGGACAAAGATCTTCCTGTGGAAAAGTATTTCTTGGAGAGGCCACCTGTGAGTGAGCCACCC AGTGACCAGGGGGTGGTGGACACGCCACACAGCCCCACCCTCCGGCTAGACAGGAAACGCAAACTGTCAG GTGACAGCACCCACACTGAGACCGCTGTGGAAGAGCTAGCTGAGGACCCTCTGAAAGCCAAGCGAAGACG GATCTCCAAAGGGCTCCATCCTAAAAAGCAACGCCACTTGCTGCACCTTAGAGAACGGTGGGAGCAGCAG GTGTCGGCAGCCGAGAGCAAACCTGGCCGGCAAAGCAGGAAGGAAGTGGCCCAGGCTGTTCAGCCTGAGG TCACCTCCCAGGGGACTAACATCACTGAAGAGAAGCCTGGCAGGAAAAAGGCAGAGGCCAAAGGCAACAG AGGCTGGTCTGAAGAGTCGCTCAAATCCTGTGACAATGAGCAAGGCTTGCCTGTGCTCTCAGGCTCTCCG CCCATGAAGAGCCTTTCATCCACCAATGCAAGTGGCAAAAAGCAGACTCAGCCAAGCTGCACGCCAGCCT CAAGGCTGCCTGCCAAACAGCAGAAAATTAAAGAAAGCCAGAAGACAGATGTGCTGTGCACAGGGGAAGA CGAGGATTGCCAGGCTGCCTCCCCGCTGCAAAAATACACAGACAACATCGAGAAGCCATCCGGGAAGAGA CTGTGCAAAACCAAGCACTTGATACCTCAGGAGTCCAGGAGGAGCTTGCAGATCACAGGCGACTACTATG TGGAGAACACTGACACTAAGATGACTGTGCGGAGATTCAGAAAACGACCTGAGCCCAGTTCGGACTACGA TTTGTCACCACCAGCCAAGCAGGAGCCAAAGCCCTTTGACCGTCTACAACAATTGCTACCGGCCACCCAG GCCACCCAGCTGCCACGCTCAAATTCTCCTCAAGAGACCACCCAGTCTCGCCCCATGCCGCCAGAAGCTC GGAGACTTATTGTCAATAAGAATGCTGGCGAGACCCTCCTGCAGCGGGCTGCCCGGCTAGGCTATGAGGA AGTGGTCTTATATTGCCTGGAGAACAAGGTCTGTGATGTCAATCATCGAGACAATGCTGGCTACTGTGCT CTGCATGAAGCTTGCGCCAGGGGATGGCTCAACATCGTGCGCCACCTCCTTGAATATGGCGCTGATGTCA ACTGCAGTGCCCAGGATGGAACCAGGCCTCTGCATGATGCTGTGGAGAATGACCACTTAGAAATTGTACG ATTGCTTCTTTCCTATGGTGCTGACCCTACTTTGGCCACATACTCAGGGAGAACCATCATGAAAATGACC CACAGTGAACTTATGGAGAAGTTTTTAACAGACTATTTAAATGACCTGCAGGGTCGCAGTGAAGATGACA CCAGCGGCGCTTGGGAGTTCTATGGCAGCTCTGTGTGTGAACCAGATGACGAAAGTGGGTACGATGTTTT GGCTAATCCCCCAGGACCAGAAGACCCAGATGAGGAAGAGGATACCTATAGCGATTTGTTTGAGTTTGAG TTTGCTGAAAGCTCCCTCTTGCCGTGTTATAACATCCAAGTGTCTGTCGCTCAGGGGCCACGAAACTGGC TTCTGCTCTCAGACGTGCTCAAGAAGTTGAAAATGTCCTCCCGGATATTCCGTAGCAATTTTCCCAATCT GGAAATTGTCACCATTGCAGAGGCGGAGTTTTACCGACAGGTCTCAACAAGTCTCTTGTTCTCCTGCCCC AAAGACCTGGAAGCCTTCAATCCTGAAAGCAAGGAGCTGTTGGATCTGGTGGAATTCACCAATGAGCTCC AGACACTGCTGGGCTCCTCTGTGGAGTGGCTCCACCCTAGTGACACGGGCCATGAAAACTACTGG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG212070 representing BC058656 Red=Cloning site Green=Tags(s) MLSATPLYGNVHSWMNSERVRMCGTSEDRKIPVNDGDASKARLELREETPLSHSVVDTSGAHRIDGLAAL SMDRTGLIREGLRVPGNIVYSGLCGLGSEKGREATPSSLSGLGFSSERNPEMQFKPNTPETVEASAVSGK PPNGFSAIYKTPPGIQKSAVATAESLGLDRPASDKQSPLNINGASYLRLPWVNPYMEGATPAIYPFLDSP NKYSLNMYKALLPQQSYGLAQPLYSPVCTSGERFLYLPPPHYVNPHIPSSLASPMRLSTPSASAAIPPLV HCSDKSLPWKMGVNPGNPVDSHSYPHIQNSKQPRVTSAKAVNSGLPGDTALLLPPSPRPSARVHLPTQPA AETYSEFHKHYPRISTSPSVTLTKPYMTANSEFSTSRLSNGKYPKALDGGDCAQSMPGHTRKTTVQDRKD GGSPPLLEKQTVTKDVTDKPLDLSSKVVDADASKGDHMKKMAPTVLVHSRAASGLVLSGSEIPKETLSPP GNGCSIYRSEIISTAPSSWVVPGPSPNEENNGKSLSLKNKALDWAIPQQRSSSCPRMGGTDAVVTNVSGS VSSSGRPASASPAPNANANADGTKTSRSSVDTTPSVIQHVGQPSSTPAKHGGSTSSKGAKANPEPSFKAS ENGLPPTSIFLSPNEAFRSPAIPYPRSYLPYAAPEGIALSPLSLHGKGPVYPHPVLLPNGSLFPGHLAPK PGLPYGLHTSRPEFVTYQDALGLGMVHPMLIPHTPIEITKEEKPERRSRSHERARYEDPTLRSRFSEMLE ASSTKLHPEVPTDKNLKPNSSWNQGKTGVKSDKLVYVDLLREEADTKTDAGAPKAGLVAENVGQDTEATK PSADPVIQQRREFISLREELGRITDFHESFTFKQASSQPVFSLGKDSGAAGTNKENLGVQVATPFLETAL GSEGPAVTFGKTQEDPKPFCVGGAPPNMDVTPAYTKEGTDEAESNDGKVLKPKPSKLAKRIANSAGYVGD RFKCVTTELYADSSQLSREQRALQMEGLQEDSILCLPAAYCERAMMRFSELEMKEREGSHPATKDSEVCK FSPADWERLKGNQEKKPKSVTLEEAIADQNDSERCEYSTGNKHDLFEAPEDKDLPVEKYFLERPPVSEPP SDQGVVDTPHSPTLRLDRKRKLSGDSTHTETAVEELAEDPLKAKRRRISKGLHPKKQRHLLHLRERWEQQ VSAAESKPGRQSRKEVAQAVQPEVTSQGTNITEEKPGRKKAEAKGNRGWSEESLKSCDNEQGLPVLSGSP PMKSLSSTNASGKKQTQPSCTPASRLPAKQQKIKESQKTDVLCTGEDEDCQAASPLQKYTDNIEKPSGKR LCKTKHLIPQESRRSLQITGDYYVENTDTKMTVRRFRKRPEPSSDYDLSPPAKQEPKPFDRLQQLLPATQ ATQLPRSNSPQETTQSRPMPPEARRLIVNKNAGETLLQRAARLGYEEVVLYCLENKVCDVNHRDNAGYCA LHEACARGWLNIVRHLLEYGADVNCSAQDGTRPLHDAVENDHLEIVRLLLSYGADPTLATYSGRTIMKMT HSELMEKFLTDYLNDLQGRSEDDTSGAWEFYGSSVCEPDDESGYDVLANPPGPEDPDEEEDTYSDLFEFE FAESSLLPCYNIQVSVAQGPRNWLLLSDVLKKLKMSSRIFRSNFPNLEIVTIAEAEFYRQVSTSLLFSCP KDLEAFNPESKELLDLVEFTNELQTLLGSSVEWLHPSDTGHENYW TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC058656
ORF Size	5177 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC058656, AAH58656
RefSeq Size	6358 bp
RefSeq ORF	5177 bp
Locus ID	71458
Gene Summary	Transcriptional corepressor. May specifically inhibit gene expression when recruited to promoter regions by sequence-specific DNA-binding proteins such as BCL6 and MLLT3. This repression may be mediated at least in part by histone deacetylase activities which can associate with this corepressor. Involved in the repression of TFAP2A; impairs binding of BCL6 and KDM2B to TFAP2A promoter regions. Via repression of TFAP2A acts as a negative regulator of osteo-dentiogenic capacity in adult stem cells; the function implies inhibition of methylation on histone H3 'Lys-4' (H3K4me3) and 'Lys-36' (H3K36me2) (By similarity).[UniProtKB/Swiss-Prot Function]

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