Ank3 (BC021657) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	MGC14049, AnkG, Ankyrin-3
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG212072 representing BC021657 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTGAAGAGCCAAAGGAGAAGCCCGCCAAGCCTGCTCATAGGAAGAGGAAAGGAAAAAAGTCTGATG CCAACGCAAGTTACTTAAGAGCAGCTCGGGCAGGGCACCTGGAAAAGGCCCTTGACTACATCAAAAATGG AGTGGACGTCAACATCTGTAACCAGAATGGATTGAATGCACTCCATCTTGCTTCCAAAGAAGGCCATGTG GAAGTGGTCTCTGAGCTGCTGCAGAGGGAAGCCAATGTTGATGCCGCCACAAAGAAAGGAAACACGGCCT TACACATCGCATCTTTGGCTGGGCAAGCGGAAGTGGTCAAGGTCTTGGTTACGAACGGAGCGAATGTCAA CGCACAATCTCAGAATGGCTTCACACCATTGTATATGGCAGCCCAGGAGAACCACCTGGAAGTCGTCAGG TTTCTTCTGGACAATGGCGCCAGCCAAAGCCTGGCCACAGAGGACGGCTTCACGCCATTGGCCGTGGCTC TGCAACAAGGTCATGACCAAGTCGTGTCCCTCCTGCTCGAGAACGACACGAAGGGAAAAGTGCGCCTCCC AGCCCTCCACATCGCAGCCCGGAAAGACGACACCAAGGCAGCAGCTCTGCTCCTGCAGAATGACACAAAC GCGGACGTGGAGTCAAAGAGTGGCTTCACCCCGCTCCACATAGCTGCCCACTATGGGAACATCAATGTGG CCACGTTGCTGTTAAACCGAGCGGCTGCTGTGGACTTCACCGCACGGAATGACATCACTCCCTTACACGT TGCCTCGAAGCGAGGAAATGCAAATATGGTGAAGCTATTGCTGGACCGGGGTGCGAAGATCGATGCCAAG ACCAGGGACGGTCTGACTCCGTTGCACTGTGGGGCGAGAAGTGGCCATGAGCAGGTGGTAGAGATGTTGC TTGACAGATCCGCCCCCATCCTTTCAAAAACCAAGAATGGATTGTCGCCACTGCACATGGCCACACAAGG AGACCATTTAAACTGCGTCCAACTCCTCCTCCAGCACAACGTGCCCGTGGACGACGTCACCAACGACTAC CTGACTGCCCTCCATGTGGCTGCCCACTGCGGCCATTACAAAGTTGCCAAGGTTCTTTTGGATAAGAAAG CTAGCCCCAATGCCAAAGCCCTGAATGGCTTCACCCCTCTCCATATCGCCTGCAAAAAGAACCGCATCCG AGTAATGGAACTCCTTTTGAAGCACGGTGCATCTATTCAAGCCGTAACCGAGTCGGGCCTTACCCCAATC CATGTTGCTGCCTTCATGGGACATGTAAATATCGTGTCACAGCTAATGCATCATGGAGCCTCCCCAAACA CCACCAATGTGAGAGGAGAGACGGCATTGCATATGGCGGCTCGGTCCGGACAAGCAGAAGTGGTGCGGTA TCTGGTCCAAGATGGGGCTCAGGTAGAAGCAAAAGCTAAGGATGACCAGACTCCACTCCACATCTCAGCC CGACTTGGGAAAGCTGACATAGTGCAACAACTGTTACAGCAAGGAGCATCCCCCAATGCAGCAACAACTT CTGGGTACACCCCCCTTCACCTTGCGGCCAGAGAGGGGCATGAGGATGTAGCTGCGTTCCTCCTGGATCA TGGAGCATCTTTATCCATAACAACAAAGAAGGGATTCACCCCTCTGCACGTGGCAGCCAAATACGGAAAG CTTGAAGTCGCAAGTCTCCTGCTGCAGAAGAGTGCGTCTCCCGATGCCGCAGGGAAGAGCGGGCTAACTC CACTGCATGTAGCAGCGCATTACGATAATCAGAAAGTGGCCCTTCTGCTCTTGGACCAGGGAGCCTCACC CCACGCAGCCGCAAAGAATGGCTATACACCACTGCACATCGCGGCCAAGAAGAACCAGATGGACATAGCC ACGTCCCTGCTGGAGTACGGTGCTGATGCAAACGCGGTTACCCGGCAAGGGATTGCGTCCGTCCATCTTG CGGCACAGGAAGGGCACGTGGACATGGTGTCGCTGCTCCTGAGTAGAAACGCGAATGTCAACCTGAGCAA TAAGAGCGGTCTCACCCCACTCCACCTGGCTGCTCAAGAAGACCGAGTGAATGTGGCCGAGGTCCTTGTC AACCAGGGGGCCCATGTGGATGCTCAGACAAAGATGGGCTACACCCCGCCCCATGTGGGCTGTCACTATG GAAATATCAAAATAGTCAATTTTCTGCTGCAGCATTCTGCAAAAGTTAATGCCAAGACGAAGAATGGATA CACAGCACTGCACCAGGCTGCTCAGCAGGGCCACACGCATATCATCAATGTCTTGCTTCAGAACAACGCC TCCCCCAATGAACTCACTGTGAATGGGAACACAGCTCTGGCCATCGCCCGGCGCCTTGGTTACATCTCGG TGGTTGACACACTGAAGGTCGTGACGGAGGAAATTATGACCACCACTACCATCACGGAGAAGCACAAAAT GAATGTCCCAGAAACGATGAATGAAGTCCTCGATATGTCAGACGATGAAGGTGAAGATGCCATCACAGGG GACACTGACAAGTATCTCGGGCCACAGGACCTTAAGGAGCTAGGTGATGACTCCCTGCCAGCAGAAGGTT ACGTAGGCTTCAGTCTTGGAGCCCGTTCTGCCAGCCTCCGCTCCTTCAGTTCGGATAGGTCCTACACCTT GAACAGAAGCTCCTACGCAAGGGACAGCATGATGATAGAGGAACTTCTGGTACCATCCAAAGAGCAGCAC CTGACGTTCACGAGGGAGTTTGATTCTGACTCCCTCAGACACTACAGTTGGGCAGCGGACACGTTAGATA ATGTGAACCTGGTCTCAAGCCCGGTGCATTCTGGGTTTCTGGTTAGCTTTATGGTGGACGCGAGAGGGGG CTCCATGCGAGGAAGCCGCCACCACGGGATGCGGATCATCATCCCTCCGCGAAAGTGTACGGCCCCCACC CGCATCACGTGCCGCCTGGTAAAGAGACATAAACTGGCCAACCCACCCCCCATGGTGGAAGGAGAGGGAT TAGCCAGTAGGCTGGTAGAAATGGGTCCTGCGGGGGCACAATTTTTAGGCCCCGTCATTGTGGAAATCCC TCATTTTGGGTCCATGAGGGGGAAGGAGAGAGAACTTATCGTCCTTCGGAGCGAGAACGGAGAGACCTGG AAGGAACATCAGTTTGACAGTAAAAACGAAGACCTCGCGGAGCTTCTCAATGGCATGGATGAAGAACTCG ACAGCCCGGAAGAGTTGGGTACAAAGCGCATCTGCAGAATTATCACAAAGGATTTCCCCCAGTATTTTGC CGTGGTTTCCCGGATTAAGCAGGAAAGCAACCAGATCGGTCCTGAGGGTGGGATTCTGAGCAGCACCACC GTGCCCCTCGTCCAGGCCTCCTTCCCAGAGGGCGCCTTAACCAAGAGGATCCGTGTGGGTCTCCAGGCTC AGCCCGTGCCAGAGGAAACGGTAAAAAAAATCCTTGGGAACAAAGCAACATTTAGCCCAATTGTCACGGT AGAGCCGAGGAGAAGGAAGTTCCATAAGCCGATCACCATGACCATTCCGGTGCCCCCGCCCTCGGGAGAA GGCGTGTCCAATGGGTACAAGGGGGATGCCACGCCCAACCTGCGGCTCCTCTGCAGCATCACAGGAGGCA CCTCACCAGCTCAATGGGAAGACATCACAGGAACAACCCCTCTGACGTTCATAAAGGATTGTGTGTCTTT CACAACCAACGTTTCAGCCAGATTCTGGCTGGCGGACTGCCATCAGGTGTTAGAGACCGTAGGGCTAGCC TCCCAGCTGTACAGAGAGCTGATATGCGTTCCCTACATGGCCAAGTTCGTTGTGTTTGCCAAAACAAACG ACCCGGTGGAGTCCTCGCTGAGGTGCTTCTGTATGACAGACGACAGGGTGGACAAAACCCTGGAGCAGCA GGAGAACTTCGAGGAGGTTGCCAGAAGCAAAGACATTGAGGTTCTGGAAGGAAAGCCCATCTACGTTGAT TGCTATGGAAACCTGGCCCCTCTGACCAAAGGAGGACAGCAGCTTGTTTTTAACTTTTATTCTTTCAAAG AAAACAGACTGCCATTTTCCATCAAGATCAGAGACACCAGTCAAGAGCCCTGTGGCCGCCTGTCTTTCCT GAAGGAGCCAAAGACAACAAAGGGATTACCCCAAACAGCTGTTTGCAACTTAAATATTACTCTGCCGGCA CATAAAAAGGCTGAGAAGGCAGACAGACGCCAGAGCTTTGCCTCCCTAGCTTTACGTAAGCGCTACAGCT ACTTGACTGAACCCAGCATGAGTCCGCAGAGTCCTTGTGAGCGGACGGATATCAGGATGGCGATAGTAGC CGATCACCTGGGACTTAGTTGGACAGAGCTGGCAAGGGAACTGAATTTTTCAGTGGATGAAATCAACCAA ATACGTGTGGAAAATCCCAATTCTTTAATTTCTCAGAGCTTCATGTTATTAAAAAAGTGGGTGACCAGAG ACGGAAAGAATGCCACAACTGATGCCTTAACTTCGGTCTTAACGAAGATTAACCGGATAGACATTGTAAC TCTGCTGGAAGGACCAATATTTGATTATGGGAATATTTCAGGCACCAGAAGCTTTGCAGATGAAAACAAT GTTTTCCATGACCCAGTTGATGGTTGGCAGAACGAGACGCCAAGTGGAAGCCTAGAGTCCCCAGCGCAAG CTCGAAGACTAACTGGTGGGTTACTGGACCGTCTGGATGACAGCTCTGACCAGGCTCGGGATTCTATTAC CTCATACCTCACGGGAGAACCTGGGAAGATCGAAGCAAATGGAAACCACACAGCGGAAGTCATTCCAGAA GCAAAGGCAAAACCCTACTTCCCGGAATCCCAAAACGATATAGGGAAACAGAGCATCAAGGAGAACCTGA AACCAAAAACACACGGATGTGGTCGCACTGAGGAACCAGTGTCGCCCCTCACAGCCTACCAGAAATCTCT GGAAGAAACCAGCAAGCTTGTCATAGAAGACGCACCTAAACCCTGTGTGCCTGTCGGCATGAAAAAGATG ACCAGGACTACGGCTGACGGCAAAGCCAGGCTCAACCTCCAGGAAGAAGAGGGGTCCACCAGGTCAGAGC CTAAGCAGGGAGAAGGCTATAAGGTGAAGACGAAGAAGGAAATCCGGAACGTGGAGAAGAAAACCCAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG212072 representing BC021657 Red=Cloning site Green=Tags(s) MSEEPKEKPAKPAHRKRKGKKSDANASYLRAARAGHLEKALDYIKNGVDVNICNQNGLNALHLASKEGHV EVVSELLQREANVDAATKKGNTALHIASLAGQAEVVKVLVTNGANVNAQSQNGFTPLYMAAQENHLEVVR FLLDNGASQSLATEDGFTPLAVALQQGHDQVVSLLLENDTKGKVRLPALHIAARKDDTKAAALLLQNDTN ADVESKSGFTPLHIAAHYGNINVATLLLNRAAAVDFTARNDITPLHVASKRGNANMVKLLLDRGAKIDAK TRDGLTPLHCGARSGHEQVVEMLLDRSAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDY LTALHVAAHCGHYKVAKVLLDKKASPNAKALNGFTPLHIACKKNRIRVMELLLKHGASIQAVTESGLTPI HVAAFMGHVNIVSQLMHHGASPNTTNVRGETALHMAARSGQAEVVRYLVQDGAQVEAKAKDDQTPLHISA RLGKADIVQQLLQQGASPNAATTSGYTPLHLAAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGK LEVASLLLQKSASPDAAGKSGLTPLHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPLHIAAKKNQMDIA TSLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLLSRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLV NQGAHVDAQTKMGYTPPHVGCHYGNIKIVNFLLQHSAKVNAKTKNGYTALHQAAQQGHTHIINVLLQNNA SPNELTVNGNTALAIARRLGYISVVDTLKVVTEEIMTTTTITEKHKMNVPETMNEVLDMSDDEGEDAITG DTDKYLGPQDLKELGDDSLPAEGYVGFSLGARSASLRSFSSDRSYTLNRSSYARDSMMIEELLVPSKEQH LTFTREFDSDSLRHYSWAADTLDNVNLVSSPVHSGFLVSFMVDARGGSMRGSRHHGMRIIIPPRKCTAPT RITCRLVKRHKLANPPPMVEGEGLASRLVEMGPAGAQFLGPVIVEIPHFGSMRGKERELIVLRSENGETW KEHQFDSKNEDLAELLNGMDEELDSPEELGTKRICRIITKDFPQYFAVVSRIKQESNQIGPEGGILSSTT VPLVQASFPEGALTKRIRVGLQAQPVPEETVKKILGNKATFSPIVTVEPRRRKFHKPITMTIPVPPPSGE GVSNGYKGDATPNLRLLCSITGGTSPAQWEDITGTTPLTFIKDCVSFTTNVSARFWLADCHQVLETVGLA SQLYRELICVPYMAKFVVFAKTNDPVESSLRCFCMTDDRVDKTLEQQENFEEVARSKDIEVLEGKPIYVD CYGNLAPLTKGGQQLVFNFYSFKENRLPFSIKIRDTSQEPCGRLSFLKEPKTTKGLPQTAVCNLNITLPA HKKAEKADRRQSFASLALRKRYSYLTEPSMSPQSPCERTDIRMAIVADHLGLSWTELARELNFSVDEINQ IRVENPNSLISQSFMLLKKWVTRDGKNATTDALTSVLTKINRIDIVTLLEGPIFDYGNISGTRSFADENN VFHDPVDGWQNETPSGSLESPAQARRLTGGLLDRLDDSSDQARDSITSYLTGEPGKIEANGNHTAEVIPE AKAKPYFPESQNDIGKQSIKENLKPKTHGCGRTEEPVSPLTAYQKSLEETSKLVIEDAPKPCVPVGMKKM TRTTADGKARLNLQEEEGSTRSEPKQGEGYKVKTKKEIRNVEKKTH TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC021657
ORF Size	5178 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC021657, AAH21657
RefSeq Size	6420 bp
RefSeq ORF	5180 bp
Locus ID	11735
Gene Summary	This gene encodes a member of the ankyrin protein family. Ankyrins link integral membrane proteins to the spectrin-based cytoskeleton. Ankyrin family members share a protein structure which includes three independently folded domains: the N-terminal ankyrin repeat domain, the central spectrin-binding domain, and the C-terminal rod domain. This ankyrin functions as the major ankyrin in the kidney and may play a role in the polarized distribution of many integral membrane proteins to specific subcellular sites. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

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