Myh1 (BC108329) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	MdMs, MHC2X/D, MyHC-IIx/d, MyHC-IId/x, IId, IId/x, MHC-2X/D, MYHC-IIX
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG212099 representing BC108329 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTTCCGACGCCGAGATGGCCGTTTTCGGGGAGGCTGCTCCTTACCTCCGGAAGTCTGAAAAGGAGC GAATCGAGGCTCAGAACAAGCCTTTTGACGCCAAGTCATCGGTGTTTGTGGTGGATGCTAAGGAGTCTTT TGTAAAAGCAACGGTGCAGAGCAGGGAAGGGGGGAAGGTGACAGCCAAGACCGAAGGCGGAACTACTGTA ACAGTAAAAGATGATCAGGTCTACCCCATGAACCCTCCCAAGTACGACAAGATCGAGGACATGGCCATGA TGACCCACCTGCATGAGCCCGCTGTGCTGTACAACCTCAAAGAGCGTTATGCAGCCTGGATGATCTACAC CTACTCGGGCCTGTTCTGCGTCACTGTCAACCCCTACAAGTGGCTGCCAGTGTATAACGCAGAGGTGGTG GCAGCCTACAGAGGCAAAAAGCGCCAGGAGGCCCCACCCCACATCTTCTCCATCTCTGACAATGCCTACC AGTTCATGCTGACAGATCGGGAGAACCAGTCTATTTTGATCACCGGAGAATCCGGGGCCGGGAAGACTGT GAACACGAAACGTGTCATCCAGTACTTTGCAACAATTGCAGTCACTGGGGAGAAAAAGAAGGAGGAGGCA ACTTCTGGCAAAATGCAGGGGACGCTGGAGGACCAAATCATCAGCGCCAACCCCCTGCTGGAGGCCTTTG GGAACGCCAAGACCGTGAGGAATGACAACTCTTCCCGCTTTGGTAAGTTCATCAGGATCCACTTTGGTAC CACAGGGAAACTGGCTTCTGCTGATATTGAAACATATCTTCTGGAGAAGTCTAGAGTCACTTTCCAGCTA AAAGCTGAGAGAAGCTACCACATTTTTTACCAGATTATGTCTAACAAGAAGCCGGATCTAATCGAAATGC TCCTGATCACCACCAACCCATACGACTACGCCTTTGTCAGTCAGGGGGAGATCACGGTGCCCAGCATTGA TGACCAAGAAGAGTTGATGGCCACAGATAGTGCCATTGATATTCTGGGCTTCACTTCTGATGAAAGAGTC TCCATCTATAAGCTCACAGGGGCTGTGATGCATTATGGGAACATGAAATTCAAGCAGAAGCAACGAGAGG AGCAGGCTGAGCCAGACGGCACCGAAGTTGCTGACAAGGCTGCCTATCTCCAAAATCTGAACTCTGCTGA CCTGCTCAAAGCCCTGTGTTACCCCAGGGTCAAGGTCGGCAATGAGTATGTCACCAAAGGCCAGACTGTG CAGCAGGTGTACAACTCAGTAGGCGCCCTAGCCAAAGCCGTCTATGAGAAGATGTTCCTGTGGATGGTCA CCCGCATCAACCAGCAGCTGGACACCAAGCAGCCCCGGCAGTACTTCATCGGGGTCTTGGACATCGCTGG CTTTGAGATCTTTGATTTCAACAGCCTGGAGCAGCTGTGCATCAACTTCACCAACGAGAAACTGCAACAG TTCTTCAACCACCACATGTTCGTGCTGGAGCAGGAGGAGTACAAGAAGGAGGGCATCGAGTGGGAGTTCA TTGACTTTGGGATGGACCTGGCGGCCTGCATCGAGCTCATCGAGAAGCCGATGGGCATCTTCTCCATCCT GGAAGAGGAGTGCATGTTCCCTAAGGCGACAGACACCTCCTTCAAGAACAAGCTGTATGAGCAGCATCTT GGAAAGTCCAACAACTTCCAAAAGCCCAAACCTGCCAAAGGCAAGGTTGAAGCTCACTTCTCCCTGGTGC ACTATGCGGGCACTGTGGACTACAACATCGCTGGCTGGCTGGACAAGAACAAGGACCCCCTGAATGAGAC TGTGGTGGGGCTGTACCAGAAGTCTTCAATGAAAACTCTGGCTTACCTCTTCTCTGGGGCAGCAGCAGCT GCGGAAGCAGAGTCTGGTGGCGGTGGTGGAAAGAAAGGGGCCAAGAAGAAGGGTTCCTCCTTCCAGACCG TGTCAGCCCTCTTCAGGGAGAATCTGAATAAGCTAATGACCAATCTGAGGAGCACTCACCCCCACTTTGT ACGGTGCATCATCCCCAATGAAACCAAGACTCCTGGTGCCATGGAGCACGAACTGGTCCTGCACCAGCTG AGGTGTAACGGGGTGCTGGAGGGCATCCGCATCTGCAGGAAGGGCTTCCCCAGCAGGATCCTCTATGCAG ACTTCAAGCAGAGATATAAGGTTTTAAATGCAAGTGCCATCCCTGAGGGACAGTTCATCGATAGCAAGAA GGCTTCTGAGAAGCTCCTGGGATCCATTGATATTGACCACACTCAGTATAAGTTTGGTCACACCAAGGTC TTTTTCAAAGCTGGTCTTCTGGGGCTCCTAGAGGAGATGAGAGATGACAAGTTGGCCCAGCTTATAACCC GGACCCAGGCTATGTGCAGAGGGTACTTGGCGAGAGTGGAGTACCAGAAGATGGTGGAGAGAAGAGAGTC CATCTTCTGCATCCAGTACAACGTCCGCGCCTTCATGAATGTGAAACACTGGCCCTGGATGAAGCTGTAT TTCAAGATCAAACCTCTCTTGAAGAGCGCAGAGACCGAGAAGGAGATGGCCAACATGAAGGAAGAATTTG AAAAAGCTAAAGAAAACCTTGCAAAGGCGGAGGCCAAAAGAAAAGAGCTGGAAGAAAAGATGGTAGCTCT GATGCAAGAGAAAAATGACCTGCAGCTCCAAGTTCAGTCTGAAGCAGACAGCTTGGCCGATGCAGAGGAG CGATGCGACCAGCTGATCAAAACCAAAATCCAGCTGGAGGCCAAAATCAAAGAGGTGACCGAGAGGGCCG AGGATGAGGAGGAGATCAACGCAGAGCTCACGGCCAAGAAGAGGAAGCTGGAGGACGAGTGCTCAGAGCT GAAGAAGGACATCGATGACCTTGAGCTGACACTGGCCAAGGTTGAGAAGGAGAAGCACGCCACGGAGAAC AAGGTGAAAAACCTGACAGAGGAGATGGCAGGCCTGGATGAAACCATCGCCAAGCTGACCAAGGAGAAGA AGGCCCTCCAGGAGGCCCACCAGCAGACCCTGGATGACCTGCAGGCAGAGGAGGACAAAGTCAACACCCT GACCAAAGCCAAAATCAAGCTGGAACAGCAAGTGGATGATCTTGAAGGCTCTCTGGAACAAGAAAAGAAA ATCCGGATGGATCTTGAAAGAGCCAAGAGGAAACTGGAGGGAGACCTGAAACTGGCCCAAGAGTCCACAA TGGACGTAGAAAACGATAAACAGCAGCTTGATGAGAAACTCAAAAAGAAAGAGTTTGAAATGAGCAATCT GCAGAGCAAGATTGAGGACGAGCAGGCCCTCGGCATGCAGCTGCAGAAGAAGATCAAGGAGTTGCAGGCC CGCATCGAGGAGCTGGAGGAGGAAATCGAGGCAGAGCGGGCCTCCAGGGCCAAAGCAGAGAAGCAGCGCT CTGACCTCTCCCGGGAACTGGAGGAGATCAGCGAGAGGCTGGAAGAAGCTGGCGGAGCCACTTCAGCCCA AATCGAGATGAACAAGAAGAGAGAGGCTGAGTTCCAGAAAATGCGCAGGGACCTGGAGGAGGCCACGCTG CAGCATGAAGCCACAGCAGCCACCCTGAGGAAGAAGCACGCGGACAGCGTGGCTGAGCTCGGGGAGCAGA TCGACAACCTGCAGCGGGTGAAGCAGAAGCTGGAGAAGGAGAAGAGCGAGATGAAGATGGAGATCGATGA CCTCGCTAGTAACATGGAGGTCATCTCCAAATCTAAGGGAAACCTTGAGAAGATGTGCCGCACACTGGAG GACCAAGTGAGTGAGCTGAAGACCAAGGAGGAGGAACAGCAGCGGCTGATCAATGAGCTGACTGCGCAGA GGGGGCGTCTGCAGACGGAGTCAGGTGAATACTCACGCCAGCTAGACGAAAAGGATTCATTAGTTTCCCA GCTCTCCAGGGGCAAACAGGCATTCACTCAACAGATTGAGGAGCTGAAGAGGCAGCTTGAAGAGGAGATA AAGGCCAAGAGTGCCTTAGCTCATGCTCTTCAGTCCTCCCGCCATGACTGTGACCTGCTGCGGGAACAGT ATGAAGAAGAACAGGAAGCCAAGGCTGAGCTGCAGAGGGCCATGTCCAAAGCCAACAGTGAAGTGGCCCA GTGGAGGACAAAATATGAGACGGATGCCATCCAGCGCACAGAGGAGCTGGAGGAGGCCAAGAAGAAGCTG GCTCAGCGTCTGCAGGATGCTGAGGAGCACGTAGAAGCTGTGAACGCCAAGTGCGCTTCCCTGGAGAAGA CGAAGCAGCGGCTGCAGAACGAGGTGGAGGACCTCATGATTGATGTGGAGAGGACCAACGCAGCCTGCGC CGCCCTGGACAAGAAGCAGAGAAACTTTGACAAGATCCTGGCAGAATGGAAACAGAAGTATGAGGAAACC CATGCTGAACTTGAAGCCTCCCAGAAGGAGTCCCGCTCTCTCAGCACAGAGCTGTTCAAGATTAAGAATG CTTATGAGGAATCTTTAGACCATCTCGAAACCTTGAAAAGGGAGAATAAGAATTTGCAACAGGAGATTTC TGACCTCACTGAACAGATTGCAGAAGGAGGGAAACGCATCCATGAACTGGAGAAAATAAAGAAGCAAATT GAGCAGGAAAAATCTGAGCTCCAGGCTGCTTTAGAGGAAGCAGAGGCATCTCTGGAGCACGAGGAGGGAA AAATCCTGCGCATCCAGCTGGAGCTGAACCAAGTCAAGTCTGAGATCGACAGGAAGATTGCTGAGAAGGA TGAGGAGATCGACCAGCTGAAGAGAAACCACATTAGAGTCGTGGAGTCCATGCAGAGCACGCTGGATGCT GAGATTAGGAGCAGGAATGACGCCATCAGGCTCAAGAAGAAGATGGAGGGAGACCTGAACGAGATGGAAA TCCAGCTGAACCACTCCAACCGCATGGCTGCCGAGGCCCTGAGAAACTATAGGAACACACAAGGCATCCT CAAGGACACTCAGCTGCACCTGGACGATGCTCTCCGGGGCCAGGAGGACCTGAAGGAGCAGCTGGCCATG GTTGAGCGCAGAGCCAACCTGCTGCAGGCTGAGATCGAGGAGCTGCGGGCCACCCTGGAGCAGACGGAGA GGAGCAGGAAGATTGCAGAGCAGGAGTTGCTGGATGCCAGTGAGCGCGTGCAGCTCCTCCACACCCAGAA CACCAGCCTCATCAACACCAAGAAGAAGCTGGAGACAGACATTTCCCAAATCCAGGGAGAGATGGAGGAC ATCGTCCAGGAAGCCCGCAATGCAGAAGAGAAGGCCAAGAAAGCCATCACTGATGCCGCCATGATGGCGG AGGAGCTGAAGAAGGAGCAGGATACCAGCGCCCACCTGGAGCGGATGAAGAAGAACCTGGAGCAGACGGT GAAGGACCTGCAGCACCGTCTGGATGAGGCTGAGCAGCTGGCGCTGAAGGGCGGCAAGAAGCAGATCCAG AAACTGGAGGCCAGGGTCCGTGAACTTGAAGGAGAAGTGGAAAATGAACAGAAGCGCAACGTGGAAGCTA TCAAGGGTCTGCGCAAACATGAGAGAAGAGTGAAAGAACTCACTTATCAAACTGAGGAAGACCGCAAGAA TGTTCTCAGGCTCCAGGACCTTGTGGACAAACTGCAATCAAAGGTCAAGGCCTACAAGAGACAAGCTGAG GAAGCGGAGGAACAATCCAATGTCAACCTGGCCAAATTCCGCAAGATCCAGCACGAGCTGGAGGAAGCCG AGGAGCGGGCTGACATCGCGGAGTCCCAGGTCAACAAGCTGCGGGTGAAGAGCCGGGAGGTTCACACCAA AATCATAAGCGAAGAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG212099 representing BC108329 Red=Cloning site Green=Tags(s) MSSDAEMAVFGEAAPYLRKSEKERIEAQNKPFDAKSSVFVVDAKESFVKATVQSREGGKVTAKTEGGTTV TVKDDQVYPMNPPKYDKIEDMAMMTHLHEPAVLYNLKERYAAWMIYTYSGLFCVTVNPYKWLPVYNAEVV AAYRGKKRQEAPPHIFSISDNAYQFMLTDRENQSILITGESGAGKTVNTKRVIQYFATIAVTGEKKKEEA TSGKMQGTLEDQIISANPLLEAFGNAKTVRNDNSSRFGKFIRIHFGTTGKLASADIETYLLEKSRVTFQL KAERSYHIFYQIMSNKKPDLIEMLLITTNPYDYAFVSQGEITVPSIDDQEELMATDSAIDILGFTSDERV SIYKLTGAVMHYGNMKFKQKQREEQAEPDGTEVADKAAYLQNLNSADLLKALCYPRVKVGNEYVTKGQTV QQVYNSVGALAKAVYEKMFLWMVTRINQQLDTKQPRQYFIGVLDIAGFEIFDFNSLEQLCINFTNEKLQQ FFNHHMFVLEQEEYKKEGIEWEFIDFGMDLAACIELIEKPMGIFSILEEECMFPKATDTSFKNKLYEQHL GKSNNFQKPKPAKGKVEAHFSLVHYAGTVDYNIAGWLDKNKDPLNETVVGLYQKSSMKTLAYLFSGAAAA AEAESGGGGGKKGAKKKGSSFQTVSALFRENLNKLMTNLRSTHPHFVRCIIPNETKTPGAMEHELVLHQL RCNGVLEGIRICRKGFPSRILYADFKQRYKVLNASAIPEGQFIDSKKASEKLLGSIDIDHTQYKFGHTKV FFKAGLLGLLEEMRDDKLAQLITRTQAMCRGYLARVEYQKMVERRESIFCIQYNVRAFMNVKHWPWMKLY FKIKPLLKSAETEKEMANMKEEFEKAKENLAKAEAKRKELEEKMVALMQEKNDLQLQVQSEADSLADAEE RCDQLIKTKIQLEAKIKEVTERAEDEEEINAELTAKKRKLEDECSELKKDIDDLELTLAKVEKEKHATEN KVKNLTEEMAGLDETIAKLTKEKKALQEAHQQTLDDLQAEEDKVNTLTKAKIKLEQQVDDLEGSLEQEKK IRMDLERAKRKLEGDLKLAQESTMDVENDKQQLDEKLKKKEFEMSNLQSKIEDEQALGMQLQKKIKELQA RIEELEEEIEAERASRAKAEKQRSDLSRELEEISERLEEAGGATSAQIEMNKKREAEFQKMRRDLEEATL QHEATAATLRKKHADSVAELGEQIDNLQRVKQKLEKEKSEMKMEIDDLASNMEVISKSKGNLEKMCRTLE DQVSELKTKEEEQQRLINELTAQRGRLQTESGEYSRQLDEKDSLVSQLSRGKQAFTQQIEELKRQLEEEI KAKSALAHALQSSRHDCDLLREQYEEEQEAKAELQRAMSKANSEVAQWRTKYETDAIQRTEELEEAKKKL AQRLQDAEEHVEAVNAKCASLEKTKQRLQNEVEDLMIDVERTNAACAALDKKQRNFDKILAEWKQKYEET HAELEASQKESRSLSTELFKIKNAYEESLDHLETLKRENKNLQQEISDLTEQIAEGGKRIHELEKIKKQI EQEKSELQAALEEAEASLEHEEGKILRIQLELNQVKSEIDRKIAEKDEEIDQLKRNHIRVVESMQSTLDA EIRSRNDAIRLKKKMEGDLNEMEIQLNHSNRMAAEALRNYRNTQGILKDTQLHLDDALRGQEDLKEQLAM VERRANLLQAEIEELRATLEQTERSRKIAEQELLDASERVQLLHTQNTSLINTKKKLETDISQIQGEMED IVQEARNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNLEQTVKDLQHRLDEAEQLALKGGKKQIQ KLEARVRELEGEVENEQKRNVEAIKGLRKHERRVKELTYQTEEDRKNVLRLQDLVDKLQSKVKAYKRQAE EAEEQSNVNLAKFRKIQHELEEAEERADIAESQVNKLRVKSREVHTKIISEE TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC108329
ORF Size	5826 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC108329, AAI08330
RefSeq Size	6058 bp
RefSeq ORF	5828 bp
Locus ID	17879
Gene Summary	Muscle contraction.[UniProtKB/Swiss-Prot Function]

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