Tro (BC075630) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	Tnn, magphinin
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG212111 representing BC075630, codon optimized. Due to the complexity of BC075630, the ORF clone is codon optimized for mammalian Expression. The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical. Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACAGGAGGAACGACCATGGCCACCGAGTGCCAACATTCCAAGGGCCACTGCCCCCTGCAGGAAGCC TCGGTCTTCCATTCTCTCCTGACGTGCAGAGCGAAACGACAGAAAAAGATCCCCCAATTGCCTCTAGGTC CAAAAAAAACAAAAATAAAAAAAATTCCATCAAACCAATGGATAAGACGACCCCTGCCCCACCCCCCGTC CCCAGCGCCAATGACAACGCCTCCAACAAACCGAAAGTCACACTGCAGGCTCTGAACTTGCCCATGTTTA CGCAGATTTCCCAGGCCTCAGCTACTACCGAAGCTCCGAACATTCAAGCTTCCGTGACAAGTCAGACCCA AAAAGCTAAAACCATGCGCGTGACACCCAAAGTCTCCCTTACCGGCTCTGAGGACGCCACAACGCAACTG AAACCGCCATTGCAAGCTTTGAATCTCCCAGTAACTACACCCACTATCCAGACGCCAGTAGCAAATGAGA GCGCCAATAGCCTTGCATCCACTGCTGTCAATAAAAGTAAAAAGGCCTCCACAGCCAATAACGCTGCCAA CAAAACGGTTCCTTCCGCCGCGGAGATCTCTCTCGCTTCTGCTGCTACCCATACAGTGACCACACAAGGA CAGGCTGCTAAGGAGACAGGCAGTATCCAAACCATCGCCGCGACTGCCCGATCCAAGAAAAACTCCAAGG GCAAACGGACCCCTGCAAAGACAACAAATACAGATAATGAGTACGTCGAAGCTAGTAATGCCATCGAGGC CTCCAGCCGACAGATCGGGGCAAGCGGCAGACAGACGGAAGCTAGTAATAGGCAGATCGAGGCAAGCTCC CGGCAGACCGAGGCCTCTAACAGACAGACTGAGGCTTCCTCCCGGCAGACTGAGGCTTCCTCACGACAGA CCGAAACGAGCAACCGGCAAATAGGCGCAAGCAATAGGCAGATAATGGCTTCCAATCGCCAGATAGGCGC ATCCAATCGCCAGATCGAGGCCAGCAATCGACAAATCGGCGCCAGTAACCGACAGACTGAGGTGTCTAGT CGGCAAATCGAGGCTAGCAATCGCCAAATAGGAGCAAGTAACCGGCAGACGGAGGCCAGCAATCGACAAA TAGGGGCTTCCAACCGCCAGACCGAGGCCTCCAACCGGCAGATTGGCGCTTCAAACCGCCAAACCGACGC ATCTAATCGGCAGACCGATGCCAGCAATAGACAGACCGAAGCTAGTAGCCGCCAGACAGAGGCATCTTCT AGGCAGACCGAAGCCAGTTCTCGCCAGACAGAGGCCAGCTCCCGCCAAATTGAGGCATCAGCTGCAGCCG TAAGACCCAAAAAACCCAGAGGCAAGAAGGGGAATAATAAAGGTTCCAACTCCGCATCCGAGCCAAGCGA AGCTCCACCTGCAATCCAGACTGTCACTAACCATGCTCTCTCCGTGACCGTTAGGATTCGGCGAGGCAGT AGAGCCCGGAAGGCAGCTAACAAGAACAGAGCAACAGAATCTCAGGCGCAGATCGCCGAGCAGGGGGCAC AGGCCAGTGAGGCCAGCATTTCCGCGCTGGAAACTCAGGTTGCTGCAGCCGTCCAGGCACTGGCCGACGA CTATCTCGCTCAGCTGTCTCTGGAGCCCACTACAAGGACCAGGGGAAAGAGAAACCGAAAGAGCAAGCAC CCAAATGGGGAGGAGAGGACCGGAAATAACTATCGGAGAATTCCTTGGGGGCGGCGCCTTCCACCACCGC GGGATGTCGCCATACTTCAGGAACGGGCTAACAAGTTGGTTAAGTACTTGCTGGTCAAGGATCAGACCAA AATCCCCATCAAACGATCTGATATGCTCAAGGACGTTATACAGGAATATGAAGATTATTTCCCAGAGATT ATTGAACGAGCTTCCTATGCTCTGGAGAAAATGTTCCGCGTTAACCTCAAGGAAATTGACAAGCAGAATA ATTTGTACATTCTGATCAGCACCCAGGAATCTAGCGCGGGTATTATGGGCACAACAAAAGATACCCCAAA GCTGGGTCTTCTTATGGTTATACTGTCAGTGATCTTCATGAATGGTAACAAAGCCAGCGAAGCTGTCATC TGGGAAGTGCTGCGCAAACTGGGACTGCACCCTGGGGTGAAACACAGCCTGTTCGGAGAGGTCAAGAAAC TGATTACCGATGAGTTTGTTAAACAGAAGTACCTGGAATACAAAAGGGTCCCAAACAGCCGCCCCCCAGA GTATGAGTTTTTCTGGGGGCTGAGGTCTTATCACGAAACATCCAAAATGAAGGTGCTCAAGTTCGCGTGC AAGGTGCAAAAAAAGGACCCCAAGGACTGGGCTGCGCAATACAGGGAGGCCGTCGAAATGGACATTCAGG CTGCTGCAGTGGCTGTCGCCGAGGCCAAGGCCAGGGCAGAGGCCCGCGCTCAAATGGGAATCGGCGAAGA GGCCGTCGCTGGCCCTTGGAACTGGGATGACATGGATATAAACTGCCTGACCCGGGAGGAGCTGGGAGAC GACGCTCAGGCTTGGTCCCGGTTCTCCTTCGAGATCGAACCCAGGGCACAGGAAAACGCGGACCCTACAA CCAATGTACTGTTCAATCAGGGTGCCACAACCCGGAACTCCTTCTCTGATGGCGCTGGGATTTCCTTCGG CGGTATCACCAATCCCTCTGGAGGCTTTGGCGGGATTTCTAACCCCTCAGGGGGTTTTGGCGGCATCTCC AACCCTTCTGGTGGCTTCGGGGGCATTTCCAACCCCAGTGGCGGGTTTGGCGGGATTTCTAATCCTAGTG GGGGCTTTGGTGGCATAAGCAACCCCAGCGGCGGGTTCGGTGGAATCAGCAATCCCAGTGGTGGTTTCGG TGGTATAAGTAACCCATCTGGTGGGTTCGGCGGAATCTCTAACCCATCCGGAGGGTTTGGAGGTATAAGT AACCCTTCAGGCGGCTTTGGAGGAATTAGTAACCCCAGCGGAGGATTCGGTGGGATCTCAAATCCTTCCG GCGGGTTTGGGGGTATCTCCAACCCTTCCGGTGGGTTCGGGGGGATTAGCAACCCCTCCGGAGGCTTTGG GGGCAGGAACTCTATCACCTTCGGAAGTGTCCCAAACACATCCGCTAATTTTTCCTCCGCCCCATCTATC AGTTTCGGAGACACCCCCAACACGTCCACAAGCTTCTCAGGTGGTGCTAATTCCAGCTTTTCCGGAACCC CGTCAACTAGCGCACCGTTTTGTAATACTGCCTCAATATCTTTCGGCGGTGCTCCCAGCACCAGCACATC CTTTTCCACCGCCTCAATCAGTTTTGGCGGCGCACCAAGCACCAGCACCTCTCTGAGCACCGCTTCTATA AGCTTCGGGGGCGCACCAAGTACGTCTACAAGCTTCTCCACGGCTTCAATTTCTTTTGGGGGGGCTCCAA GCACAAGCACATCACTGTCTACCGCATCTATCTCATTCGGAGGGGCACCAAGCATCAATTCTTCCAGTGG GGGTTCTTCCGTGAGTTTCGGCGGTGCTCCAACAACCTCCACTTCCTTCAGCGGGGGACCGTGTATCAGC TTCGGCGGTGCCCCATGCACTACTGCCAGTATTAGTGGTGGAGCCAGTAGTGGCTTCGGGAGTACCCTTT GCAGTACTAACCCAGGCTTCAGCGCTCTGAGTACCAATACTTCTTTTGGGTCTGCGCCTACCACCTCCAC CGTCTTTTCAGGGGCCGTAAGCACAACAACAGGGTTTGGAGGGACACTTTCCACGTCCGTGTGCTTCGGA TCTTCTCCATATTCAGGCGCTGGCTTCGGTGGCACACTGAGCACCAGCATTTCATTCGGGGGAAGCCCGA GCACAAATACAGGGTTTGGCGGGACCCTCTCAACAAGCGTTTCCTTCGGGGCGAGTAGTTCTACCTCATC TGATTTCGGGGGCACCCTCAGCACATCCGTCAGCTTCGGAGGCTCTTCTGGCGCCAATGCAGGCTTTGGC GGGACTCTTAACTCTTCCACCTCCTTCGGGGGGGCCATATCAACCTCTACTGGTTTCGGGAGCGCTCTGA ACAACAGCGCCAACTTTGGGGGTGCCATCTCAACTAGTTTTTCTGGAGTATTGAATTCTAGTGCATCCTT CGGGGGCGCAATTAACACATCTGCCGGATTTGGAAGTACTCTCAATTCATCCGCCTCATTCGGAAGCGCA CTCTCTACTAGCGCATCTTTCGGCGGTGTTCTCAACGGATCCGCTGGATTCGGCGGTGCATTGAACACTA ATGCCACATTCGGCGGTGTGCTGAACGGAAGTGCTGGATTCGGGGGAGCTATGAATACCAACGCGACCTT TGGCGGAGCCCTGAATTCTAACGCGGGTTTCGGAGGCGCCATTAGTACCTCCACCAACTTCGGAGGTGCC CTTAATAACTCAGCCGGGTTCGGGGGGGCGATGAACACAAGTGCTAGCTTCGGGGGCGCCCTGAATAATA GCGCAGGCTTTGGGGGAGCAATCTCAACTAATGCCACTTTTGGAGGCGCTTTGAATAATAGTGCGGGCTT CGGCGGTGCTATTAGCACAAACGCGACCTTCGGAGGCGCTCTTAATAACTCTGCAGGATTCGGAGGAGCT ATCTCCACTTCTGCGAGTTTCGGTGGAACATTGAACAACTCTGCTTCTTTTGGTGGAGCCATCAACACCT CAGCGAGCTTCGGTGGAGTGCTCAATAACTCCGCCGGCTTTGGGGGCGCAATTAATACTTCAGCTAATTT CGGCGGTGCTCTCACTAACAGTGCTGGCTTTGGCGGTGCCATTTCTACCTCCGCCTCCTTTGGGGGCGCG TTGAATAACTCCGCTGGCTTTGGAGGCGCTATCTCAACTAGCGCTAGCTTTGGAGGTGCCCTTAATAATT CTGCCGGGTTCGGCGGTGCTATCTCCACGAATGCAAGCTTCGGAGGCGCAATCAGCAACTCTCCCGACTT TGGGGGGGCCTTCTCAACTTCTGTTGGTTTTGGCGGCACCCTCAACACGACAGATTTCGGCTCAACCCAC TCTAACTCAATTAGTTTTGGCTCTGCACCAACCACCAGTGTGAGTTTCGGAGGCAGCCACTCCACAAACT TGTGTTTTGGGGGCGCACCTTCTACGAGTCTCTGCTTTGGCAGCGCATCAAACACAAACTTGTGTTTCGG CGGGTCTAATTCAACTAACTGCTTCTCCGGAGCAACCTCCGCCAACTTTAATGAGGGGCACTCCATCTCT TTCGGGAATGGTCTGTCAACGTCCGCTGGATTTGGGAATGGATTGGGAACATCCGCCGGATTTGGATCCT CCCTGGGCACTAGTACTGGCTTCGGGGGGTCCCTGGGACCTTCCGCCAGCTTTAACGGAGGCCTCGGGAC AAGCACCGGGTTCGGGGGAGGACTGGGCACATCAACTGACTTCTCCGGAGGCCTGAACCACAACGCTGAT TTCAATGGCGGTCTTGGCAACAGCGCGGGATTCAACGGAGGGCTGAACACGAACACCGATTTTGGGGGCG AGCTGGGCACCAGTGCTGGATTCGGCGACGGACTCGGATCATCTACATCTTTCGGGGCAGGTCTTGTGAC CTCTGATGGCTTTGCAGGAAACCTCGGCACAAACACAGGGTTCGGGGGGACCCTTGGCACAGGCGCTGGG TTTTCCGTGTCCCTGAACAATGGTAATGGCTTCGGTAACGGCCCCAACGCATCATTCAACCGCGGCCTGA ATACAATTATTGGCTTTGGGTCTGGCTCAAATACCTCTAACGGCTTCACGGGGGAGCCCAATACAGGATC CAGTTTTTCAAATGGCCCCTCCAGCATTGTCGGGTTCTCTGGAGGCCCCTCAACCGGTGCAGGATTCTGT TCTGGCCCCAGCACAGGCGGGTTTGGAGGGGGCCCTTCCACTGGGCCCGGATTTGGAGGCCCCTCCACCG GACCCGGCTTTGGCGGCCCCAGCACCGGTGGTGGATTCGGGGGTCCGAACACTGGGGGCGGATTCGGCGG CCCCAGCACAGGGGGGGGCTTTGGCGGCCCATCAACCGGAGGGGGATTTGGAGGACCATCTACAGGAGGG GGGTTTGGCGGGCCTAGCACAGCCGCTGGCTTCGGCTCCGGTCTTTCTACCTCCACAGGATTTGGCGGCG GACTGAATACCTCCGCTGGCTTTAGTGGCGGACCCCCGAGTACAGGAACGGGCTTCGGAGGCGGAGCCTC ATCACACGGGGGGTGCGGGTTTCCCTATGGG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG212111 representing BC075630 Red=Cloning site Green=Tags(s) MDRRNDHGHRVPTFQGPLPPAGSLGLPFSPDVQSETTEKDPPIASRSKKNKNKKNSIKPMDKTTPAPPPV PSANDNASNKPKVTLQALNLPMFTQISQASATTEAPNIQASVTSQTQKAKTMRVTPKVSLTGSEDATTQL KPPLQALNLPVTTPTIQTPVANESANSLASTAVNKSKKASTANNAANKTVPSAAEISLASAATHTVTTQG QAAKETGSIQTIAATARSKKNSKGKRTPAKTTNTDNEYVEASNAIEASSRQIGASGRQTEASNRQIEASS RQTEASNRQTEASSRQTEASSRQTETSNRQIGASNRQIMASNRQIGASNRQIEASNRQIGASNRQTEVSS RQIEASNRQIGASNRQTEASNRQIGASNRQTEASNRQIGASNRQTDASNRQTDASNRQTEASSRQTEASS RQTEASSRQTEASSRQIEASAAAVRPKKPRGKKGNNKGSNSASEPSEAPPAIQTVTNHALSVTVRIRRGS RARKAANKNRATESQAQIAEQGAQASEASISALETQVAAAVQALADDYLAQLSLEPTTRTRGKRNRKSKH PNGEERTGNNYRRIPWGRRLPPPRDVAILQERANKLVKYLLVKDQTKIPIKRSDMLKDVIQEYEDYFPEI IERASYALEKMFRVNLKEIDKQNNLYILISTQESSAGIMGTTKDTPKLGLLMVILSVIFMNGNKASEAVI WEVLRKLGLHPGVKHSLFGEVKKLITDEFVKQKYLEYKRVPNSRPPEYEFFWGLRSYHETSKMKVLKFAC KVQKKDPKDWAAQYREAVEMDIQAAAVAVAEAKARAEARAQMGIGEEAVAGPWNWDDMDINCLTREELGD DAQAWSRFSFEIEPRAQENADPTTNVLFNQGATTRNSFSDGAGISFGGITNPSGGFGGISNPSGGFGGIS NPSGGFGGISNPSGGFGGISNPSGGFGGISNPSGGFGGISNPSGGFGGISNPSGGFGGISNPSGGFGGIS NPSGGFGGISNPSGGFGGISNPSGGFGGISNPSGGFGGISNPSGGFGGRNSITFGSVPNTSANFSSAPSI SFGDTPNTSTSFSGGANSSFSGTPSTSAPFCNTASISFGGAPSTSTSFSTASISFGGAPSTSTSLSTASI SFGGAPSTSTSFSTASISFGGAPSTSTSLSTASISFGGAPSINSSSGGSSVSFGGAPTTSTSFSGGPCIS FGGAPCTTASISGGASSGFGSTLCSTNPGFSALSTNTSFGSAPTTSTVFSGAVSTTTGFGGTLSTSVCFG SSPYSGAGFGGTLSTSISFGGSPSTNTGFGGTLSTSVSFGASSSTSSDFGGTLSTSVSFGGSSGANAGFG GTLNSSTSFGGAISTSTGFGSALNNSANFGGAISTSFSGVLNSSASFGGAINTSAGFGSTLNSSASFGSA LSTSASFGGVLNGSAGFGGALNTNATFGGVLNGSAGFGGAMNTNATFGGALNSNAGFGGAISTSTNFGGA LNNSAGFGGAMNTSASFGGALNNSAGFGGAISTNATFGGALNNSAGFGGAISTNATFGGALNNSAGFGGA ISTSASFGGTLNNSASFGGAINTSASFGGVLNNSAGFGGAINTSANFGGALTNSAGFGGAISTSASFGGA LNNSAGFGGAISTSASFGGALNNSAGFGGAISTNASFGGAISNSPDFGGAFSTSVGFGGTLNTTDFGSTH SNSISFGSAPTTSVSFGGSHSTNLCFGGAPSTSLCFGSASNTNLCFGGSNSTNCFSGATSANFNEGHSIS FGNGLSTSAGFGNGLGTSAGFGSSLGTSTGFGGSLGPSASFNGGLGTSTGFGGGLGTSTDFSGGLNHNAD FNGGLGNSAGFNGGLNTNTDFGGELGTSAGFGDGLGSSTSFGAGLVTSDGFAGNLGTNTGFGGTLGTGAG FSVSLNNGNGFGNGPNASFNRGLNTIIGFGSGSNTSNGFTGEPNTGSSFSNGPSSIVGFSGGPSTGAGFC SGPSTGGFGGGPSTGPGFGGPSTGPGFGGPSTGGGFGGPNTGGGFGGPSTGGGFGGPSTGGGFGGPSTGG GFGGPSTAAGFGSGLSTSTGFGGGLNTSAGFSGGPPSTGTGFGGGASSHGGCGFPYG TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC075630
ORF Size	6261 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC075630, AAH75630
RefSeq Size	6637 bp
RefSeq ORF	6263 bp
Locus ID	56191

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