Numa1 (NM_133947) Mouse Tagged ORF Clone
CAT#: MG212112
- TrueORF®
Numa1 (tGFP-tagged) - Mouse nuclear mitotic apparatus protein 1 (Numa1)
ORF Plasmid: DDK
"NM_133947" in other vectors (2)
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CNY 17,860.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | 6720401E04Rik; AA764025; AL022610; AU014979; NuMA |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG212112 representing NM_133947
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACACTCCATGCCACCCGGGCAGCTACGCTCCTTTCTTGGGTGAACAGTCTGCATGTTGCTGACCCCG TGGAGACAGTGCTGCAGCTCCAGGACTGCAGCATCTTCATCAAGATTATTAACACCATCCATGACACTAA AGAGGGGCAACAAATCCTACAGCAGCCACTACCGGAAAGACTAGACTTTGTTTGCAGTTTTCTACAGAAA AATCGGAAACATCCTTCATCTACACAATGCCTGGTATCTGTGCAGAAAGTGATAGAGGGATCCGAGATGG AACTGGCCAAGATGATAATGCTGTTTTTATATCAGTCCACCATGAGCTCCAGAAACCTCCGGGACTGGGA GCAGTTTGAATATGGGGTTCAGGCTGAGTTAGCGGTCATCCTGAAGTTTATGCTGGATCATGAGGAGTCG CTAAACCTTACTGAGGACTTAGAGAGTTTCTTGGAGAAAGTTCCTTACACCCATGCCAGCACCCTCTCAG AAGAGCTTTCTCCACCCAGCCACCAGACCAAGAGGAAGATTCGCTTCCTAGAAATACAGAGGATTGCTTC ATCTTCCAGTGAGAACAACTTCCTCTCAGGCTCTCCATCCTCCCCTATGGGCGACATACTGCAGACCCCA CAGTTCCAGATGAGACGACTGAAGAAGCAGCTGGCAGACGAGAGGAGCAACAGAGATGACCTGGAGCTGG AGCTGTCTGAGAGCCTCAAGCTCCTCACTGAGAAGGACGCGCAGATCGCCATGATGCAGCAGCGCATTGA TCACCTGGCTCTGCTGAATGAGAAGCAGGCAGCCAGCTCCCAGGAGCCCAGTGAGCTTGAGGAGCTCCGA GGCAAGAATGAAAGCCTCACTGTGCGGCTGCATGAGACTTTGAAGCAGTGTCAGAACCTGAAGACCGAGA AGAGCCAGATGGATCGAAAGATTAGCCAGCTTTCTGAGGAGAATGGGGACCTTTCCTTTAAGGTTCGAGA GTTTGCAAACCACTTGCAACAACTTCAGGGTGCATTCAACGACCTGATAGAGGAGCACAGCAAGGCTTCT CAGGAGTGGGCAGAGAAGCAGGCTCGTCTGGAAAATGAGCTCAGCACAGCCCTCCAGGATAAGAAATGTC TTGAAGAGAAGAATGAAATTCTTCAGGGAAAGCTTTCCCAGCTGGAAGATCAAGCAACTCGGCTGCAGGA GAGCCCAGCCCCGGAGAAGGGTGAGGTGCTAGGAGACGCCTTGCAGCTGGACACTCTAAAGCAAGAGGCA GCCAAACTTGCCACAGACAACACCCAGCTCCAAACCCGCGTAGAGACACTGGAGTGTGAACGTGGCAAGC AGGAAGCCCAGCTGCTTGCTGAACGGAGCCGCTTTGAAGACGAAAAGCAGCAGCTGGCCAGCTTGATTGC TGACCTTCAGAGCTCCGTCTCTAACCTCAGCCAGGCCAAAGAGGAGTTAGAGCAAGCCTCCCAGGCCCAA GGGGCTCAGCTCACTGCCCAGTTGACCTCTATGACAGGGCTCAATGCCACCCTGCAGCAGCGAGATCAAG AGCTGGCCAGTCTGAAAGAACAGGCCAAAAAGGAACAGGCTCAGATGCTACAGACCATGCAAGAGCAAGA ACAGGCTGCCCAGGGCCTCCGCCAGCAGGTGGAGCAGCTGAGCAGCAGCCTGAAGCTGAAGGAGCAGCAA TTAGAAGAGGCAGCCAAGGAGCAGGAGGCAACCAGACAGGATCACGCCCAGCAACTGGCCATCGTTGCTG AGGCTCGAGAGGCCTCTCTAAGGGAACGGGATACTGCTCGCCAGCAGCTAGAAACAGTGGAGAAGGAGAA GGATGCCAAGCTAGAGAGTCTGCAGCAGCAACTTCAGGCTGCCAATGATGCTAGGGACAATGCGCAGACC TCGGTCACACAGGCTCAGCAGGAAAAGGCAGAGCTGAGCCAAAAGATAGGAGAGCTTCATGCCTGCATTG AAGCTTCCCACCAGGAACAGCGTCAGGTTCAAGCTCGTGTGACAGAGCTAGAAGCCCAGCTGAAAGCTGA GCAGCAAAAAACAACTGAGCGAGAAAAGGTGGTTCAGGAGAAGGCCCAGCTCCAGGAGCAGCTCCGGGCC CTTGAGGAGTCTTTGAAGATTACCAAAGGCAGCCTTGAAGAAGAGAAACGCAGGGCTGCAGATGCCCTGA AGGAGCAGCAGTGCCGTGCCACTGAGATGGAGGCAGAGTCTCGAAGCCTGATGGAGCAGCGGGAGCGGGA ACAGAAGGAGTTAGAGCAAGAGAAGGCTGGGCGAAAGGGGCTGGAGGCCCGGATACAGCAGCTTGAAGAG GCTCATCAGGCTGAGACTGAAGCCTTGCGGCATGAGCTGGCAGAGGCCACAGCTTCCCAGCACAGAGCTG AGAGTGAGTGTGAGCGGCTCATCAGAGAGGTTGAGAGCCGGCAGAAGCGGTTTGAAGCTAGACAGCAAGA GGAGGCAAGGTACGGTGCCATGTTCCAGGAACAGCTGATGGCTTTGAAAGGGGAGAAGACTGGCCAAGAG GTCCAGGAGGAGGCAGTAGAGATCCACAGTGAAGGCCAGCCAGGCCAGCAGCAGAGTCAGCTAGCCCAGC TTCATGCCAGTCTGGCCAAAGCCATCCAGCAGGTCCAGGAGAAGGAAGTGAGAGCACAGAAGCTGGTAGA TGATCTCTCAGCTCTGCAGGAGAAGATGGCTGCCACTAACAAGGAGGTGGCCTGTCTGAAGACCTTGGTG CTCAAGGCAGGTGAGCAGCAGGAAACAGCCTCACTTGAGCTGCTCAAAGAGCCCCCGAGAGCTGCAAACA GAGCGTCTGACCAGCTAGGAGAACAGCAGGGACGGCCATTCTCTAGTACACACGCAGCAGTAAAAGCTAT GGAGCGTGAAGCTGAGCAGATGGGCGGTGAACTGGAGAGGCTGCGGGCAGCACTGATCAAGAGCCAGGGC CAGCAGCAAGAGGAGCGTGGGCAACAGGAGAGGGAGGTAGCACGGCTGACCCAGGAGCGGGGCCAGGCCC AGGCTGACCTGGCTCAGGAGAAGGCAGCCAAGGCAGAGCTGGAGATGCGACTGCAGAACACCCTCAATGA GCAGCGTGTGGAGTTTGCTGCCCTACAGGAAGCACTGGCCCATGCCCTGACAGAAAAGGAAGGGACAGAC CAAGAGCTTGCCAAACTTCGAGGGCAGGAGGCAGCTCAAAGAACAGAGCTGAAGGAGCTTCAACAGACTT TGGAACAGCTGAAAATACAGTTAGTGAAGAAAGAAAAAGAGCACCCTGCAGGGGGTGCCAGTGGAGAAGA TGCTTCTGGCCCAGGAACCCAGTCGGAGACAGCTGGAAAGACAGATGCACCAGGCCCTGAGTTGCAGGCA CTTCGGGCAGAGATCAGTAAGCTAGAGCAGCAGTGTCAGCAGCAGCAGCAACAGGTGGAAGGCCTGACAC ACAGCCTCAAGTCTGAGCGGGCTTGCCGGGCCGAGCAAGACAAAGCCCTGGAGACACTGCAGGGCCAGTT AGAAGAGAAGGCTCGAGAGCTAGGGCATAATCAGGCTGCCTCAGCTTCAGCTCAGAGAGAGTTGCAAGCC CTCCGTGCTAAAGCCCAGGACCATAGCAAAGCTGAGGAAGAGTGGAAGGCCCAGGTAGCCCGGGGCCAGC AGGAGGCTGAAAGAAAAAGCAGTCTTATCAGCAGCTTGGAAGAGGAGGTATCCATCCTGAATCGCCAAGT CCTAGAGAAGGAAGGGGAGAGCAAGGAGTTGAAGCGCCTGGTTGTAGCTGAATCCGAGAAGAGCCAGAAG CTGGAAGAGAGGCTGCGCCTGCTTCAAGTAGAAACAGCCAGCAATAGTGCCAGGGCAGCAGAACGCAGCT CAGCTCTACGAGAGGAGGTCCAGAGCCTCCGGGAAGAGGTGGAGAAACAGCGTGTAGTTTCAGAGAACTC GCGGCAGGAGTTGGCCTCACAAGCAGAGCGAGCTGAGGAGTTGGGCCAAGAATTGAAGGCCTGGCAGGAG AAGTTTTTCCAGAAGGAGCAAGCTCTCTCTGCCCTGCAGCTGGAGCACACCAGCACACAGGCCCTGGTAA GTGAGCTGCTGCCTGCCAAGCACCTTTGTCAGCAGCTGCAAGCTGAGCAAGCGGCTGCCGAGAAGCGCTT CCGGGAGGAGCTCGAGCAGAGCAAGCAGGCAGCTGGTGGGCTCCAGGCAGAGCTGATGCGGGCCCAGCGG GAGCTCGGAGAGCTCGGATCTTTGCGGCAGAAGATAGTTGAGCAGGAGCGAGCAGCCCAGCAGCTGCGGG CAGAGAAGGCCAGCTATGCAGAGCAGCTGAGCATGCTGAAGAAGGCCCATGGCCTGCTGGCAGAGGAGAA CCGGGGGCTGGGAGAGCGGGCCAACCTTGGCCGGCAGTTTCTGGAAGTAGAACTAGATCAAGCCCGGGAG AAATATGTCCAGGAGTTGGCTGCTGTGCGTACTGATGCTGAGACCCACTTGGCTGAAATGCGACAAGAAG CACAGAGTACTAGCCGGGAGCTGGAGGTGATGACTGCCAAGTACGAAGGTGCCAAGGTGAAGGTCCTAGA GGAAAGGCAGAGGTTCCAAGAAGAGAGGCAGAAACTCACTGCCCAGGTGGAAGAACTAAGTAAGAAGCTG ACAGAGCATGACCAAGCCAGCAAGGTGCAGCAACAGAAGCTAAAGGCTTTCCAGGCCCAGAGAGGTGAAA GCCAGCAAGAGGTCCAGCGCCTCCAGACCCAGCTGAACGAGCTGCAGGCCCAGCTGAGCCAGAAGGAGCA GGCAGCTGAGCATTACAAGCTCCAGATGGAGAAAGCCAAGACCCATTATGATGCCAAGAAGCAGCAGAAC CAAAAGCTGCAGGAACAGCTTCAAGACTTGGAGGAACTGCAGAAGGAGAACAAGGAGCTGCGGTCTGAAG CTGAACGTCTGGGCCGTGAGCTGCAGCAGGCAGGGCTGAAGACCAAGGAAGCTGAACAGACCTGCCGTCA CCTTACAGCCCAGGTGCGCAGCCTGGAGGCCCAGGTTGCCCATGCAGACCAGCAGCTTCGTGACTTGGGC AAATTCCAGGTGGCAACTGATGCCTTAAAGAGCCGTGAGCCTCAAGTGAAACCCCAGCTGGACTTAAGTA TTGACAGCCTGGATCTGAGTTTGGAGGAGGGAACCCCATGCAGTGTGGCTAGCAAGCTGCCTCGTACCCA ACCAGATGGTACCAGTGTCCCTGGAGAGCCAGCTTCACCTATCTCCCAGCGCCTGCCCCCCAAAGTGGAA TCCCTGGAGAGCCTCTACTTCACCCCCACTCCTGCTCGGGGTCAGGCCCCCTTGGAGACTAGCCTTGACT CTCTGGGGGATGCCTTCCCTGACTCCGGCCGTAAGACCCGCTCTGCTCGCCGCCGTACCACACAAATCAT CAACATCACTATGACCAAGAAATTAGAATTGGAAGAGCCAGACAGCGCCAACTCATCCTTCTACAGCACG CAGTCTGCTCCTGCTTCCCAGGCCAACTTGCGAGCCACTTCCTCCACTCAGTCTCTGGCCCGCTTGGGTT CTCCTGATGATGGCAACTCCGCTCTGCTTAGCCTGCCTGGCTACCGGCCTACCACACGAAGCTCTGCTCG TCGCTCCCAGGCCAGGATGTCCAGTGGGGCCCCCCAAGGGAGGAATAGCTTCTACATGGGCACTTGCCAG GATGAGCCCGAGCAGCTGGATGATTGGAACCGCATCGCAGAGTTGCAGCAGCGCAACCGAGTATGCCCCC CGCACCTGAAGACCTGCTACCCTCTGGAGTCCAGGCCTACCCTGAGCCTGGCCACCATCACTGATGAGGA GATGAAGACTGGAGACCCCAGGGAAACCTTGCGTAGAGCCAGCATGCAGCCAGCTCAGATTGCTGAGGGT GTTGGCATAACTACCAGGCAGCAGCGCAAACGAGTTTCCTCTGAGACCCACCAGGGCCCTGGCACTCCTG AGTCCAAGAAAGCTACCAGCTGCTTTCCACGCCCCATGACCCCCAGGGACCGGCATGAAGGACGTAAGCA GAGCAGCACTGCTGACACCCAGAAGAAAGCAGCTCCAGTTCTCAAACAGGCGGACCGGCGTCAGTCAATG GCTTTCAGCATCCTTAACACACCTAAGAAGCTGGGAAATAGTCTTCTAAGGCGAGGAGCTTCAAAGAAGA CCCCAGCAAAGGTCTCCCCCAATCCTCGCAGTGGAACCCGCCGTTCTCCACGCATTGCCACCACCACAAC TGGCACTGCTACTGTTGCCACCACTCCTCGGGCCAAGGGCAAGGTGAAGCAT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG212112 representing NM_133947
Red=Cloning site Green=Tags(s) MTLHATRAATLLSWVNSLHVADPVETVLQLQDCSIFIKIINTIHDTKEGQQILQQPLPERLDFVCSFLQK NRKHPSSTQCLVSVQKVIEGSEMELAKMIMLFLYQSTMSSRNLRDWEQFEYGVQAELAVILKFMLDHEES LNLTEDLESFLEKVPYTHASTLSEELSPPSHQTKRKIRFLEIQRIASSSSENNFLSGSPSSPMGDILQTP QFQMRRLKKQLADERSNRDDLELELSESLKLLTEKDAQIAMMQQRIDHLALLNEKQAASSQEPSELEELR GKNESLTVRLHETLKQCQNLKTEKSQMDRKISQLSEENGDLSFKVREFANHLQQLQGAFNDLIEEHSKAS QEWAEKQARLENELSTALQDKKCLEEKNEILQGKLSQLEDQATRLQESPAPEKGEVLGDALQLDTLKQEA AKLATDNTQLQTRVETLECERGKQEAQLLAERSRFEDEKQQLASLIADLQSSVSNLSQAKEELEQASQAQ GAQLTAQLTSMTGLNATLQQRDQELASLKEQAKKEQAQMLQTMQEQEQAAQGLRQQVEQLSSSLKLKEQQ LEEAAKEQEATRQDHAQQLAIVAEAREASLRERDTARQQLETVEKEKDAKLESLQQQLQAANDARDNAQT SVTQAQQEKAELSQKIGELHACIEASHQEQRQVQARVTELEAQLKAEQQKTTEREKVVQEKAQLQEQLRA LEESLKITKGSLEEEKRRAADALKEQQCRATEMEAESRSLMEQREREQKELEQEKAGRKGLEARIQQLEE AHQAETEALRHELAEATASQHRAESECERLIREVESRQKRFEARQQEEARYGAMFQEQLMALKGEKTGQE VQEEAVEIHSEGQPGQQQSQLAQLHASLAKAIQQVQEKEVRAQKLVDDLSALQEKMAATNKEVACLKTLV LKAGEQQETASLELLKEPPRAANRASDQLGEQQGRPFSSTHAAVKAMEREAEQMGGELERLRAALIKSQG QQQEERGQQEREVARLTQERGQAQADLAQEKAAKAELEMRLQNTLNEQRVEFAALQEALAHALTEKEGTD QELAKLRGQEAAQRTELKELQQTLEQLKIQLVKKEKEHPAGGASGEDASGPGTQSETAGKTDAPGPELQA LRAEISKLEQQCQQQQQQVEGLTHSLKSERACRAEQDKALETLQGQLEEKARELGHNQAASASAQRELQA LRAKAQDHSKAEEEWKAQVARGQQEAERKSSLISSLEEEVSILNRQVLEKEGESKELKRLVVAESEKSQK LEERLRLLQVETASNSARAAERSSALREEVQSLREEVEKQRVVSENSRQELASQAERAEELGQELKAWQE KFFQKEQALSALQLEHTSTQALVSELLPAKHLCQQLQAEQAAAEKRFREELEQSKQAAGGLQAELMRAQR ELGELGSLRQKIVEQERAAQQLRAEKASYAEQLSMLKKAHGLLAEENRGLGERANLGRQFLEVELDQARE KYVQELAAVRTDAETHLAEMRQEAQSTSRELEVMTAKYEGAKVKVLEERQRFQEERQKLTAQVEELSKKL TEHDQASKVQQQKLKAFQAQRGESQQEVQRLQTQLNELQAQLSQKEQAAEHYKLQMEKAKTHYDAKKQQN QKLQEQLQDLEELQKENKELRSEAERLGRELQQAGLKTKEAEQTCRHLTAQVRSLEAQVAHADQQLRDLG KFQVATDALKSREPQVKPQLDLSIDSLDLSLEEGTPCSVASKLPRTQPDGTSVPGEPASPISQRLPPKVE SLESLYFTPTPARGQAPLETSLDSLGDAFPDSGRKTRSARRRTTQIINITMTKKLELEEPDSANSSFYST QSAPASQANLRATSSTQSLARLGSPDDGNSALLSLPGYRPTTRSSARRSQARMSSGAPQGRNSFYMGTCQ DEPEQLDDWNRIAELQQRNRVCPPHLKTCYPLESRPTLSLATITDEEMKTGDPRETLRRASMQPAQIAEG VGITTRQQRKRVSSETHQGPGTPESKKATSCFPRPMTPRDRHEGRKQSSTADTQKKAAPVLKQADRRQSM AFSILNTPKKLGNSLLRRGASKKTPAKVSPNPRSGTRRSPRIATTTTGTATVATTPRAKGKVKH TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_133947 |
ORF Size | 6282 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_133947.3, NP_598708.3 |
RefSeq Size | 7214 bp |
RefSeq ORF | 6285 bp |
Locus ID | 101706 |
UniProt ID | E9Q7G0 |
Gene Summary | Microtubule (MT)-binding protein that plays a role in the formation and maintenance of the spindle poles and the alignement and the segregation of chromosomes during mitotic cell division (PubMed:19255246, PubMed:24109598, PubMed:26765568). Functions to tether the minus ends of MTs at the spindle poles, which is critical for the establishment and maintenance of the spindle poles (PubMed:26765568). Plays a role in the establishment of the mitotic spindle orientation during metaphase and elongation during anaphase in a dynein-dynactin-dependent manner (PubMed:26765568). In metaphase, part of a ternary complex composed of GPSM2 and G(i) alpha proteins, that regulates the recruitment and anchorage of the dynein-dynactin complex in the mitotic cell cortex regions situated above the two spindle poles, and hence regulates the correct oritentation of the mitotic spindle (PubMed:24109598, PubMed:26765568). During anaphase, mediates the recruitment and accumulation of the dynein-dynactin complex at the cell membrane of the polar cortical region through direct association with phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2), and hence participates in the regulation of the spindle elongation and chromosome segregation. Binds also to other polyanionic phosphoinositides, such as phosphatidylinositol 3-phosphate (PIP), lysophosphatidic acid (LPA) and phosphatidylinositol triphosphate (PIP3), in vitro (By similarity). Also required for proper orientation of the mitotic spindle during asymmetric cell divisions (PubMed:26765568). Plays a role in mitotic MT aster assembly. Involved in anastral spindle assembly. Positively regulates TNKS protein localization to spindle poles in mitosis. Highly abundant component of the nuclear matrix where it may serve a non-mitotic structural role, occupies the majority of the nuclear volume (By similarity). Required for epidermal differentiation and hair follicle morphogenesis (PubMed:26765568).[UniProtKB/Swiss-Prot Function] |
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