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Fign (NM_021716) Mouse Tagged ORF Clone
CAT＃: MG212262
- TrueORF®
Fign (tGFP-tagged) - Mouse fidgetin (Fign), (10ug)
ORF Plasmid: DDK
"NM_021716" in other vectors (4)
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CNY 6,370.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | fi; fidget |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG212262 representing NM_021716
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATCAGTAGCACCAGTGTTTATGGCTTGAAGATGCAGTGGACGCCGGAGCATGCCCAGTGGCCAGAAC AGCACTTTGACATCACCTCGACCACTCGGTCTCCCGCCCACAAAGTTGAAGCCTATAGAGGTCATTTACA GCGCACCTACCAGTATGCCTGGGCGAATGATGACATATCTGCTCTGACTGCGTCCAACCTTCTGAAAAAA TATGCAGAGAAGTATTCTGGCATTTTGGAAGGTCCCGTAGACCGACCTGTACTCAGCAATTATTCAGACA CACCATCAGGACTCGTGAATGGTCGGAAAAATGATAGCGAACCCTGGCAGCCTTCCTTGAATTCAGAAGC CGTTTATCCTATGAACTGTGTCCCGGATGTCATCACTGCCAGCAAAGCTGGTGTCAGCTCAGCCCTCCCT CCAGTAGACGTCTCGGCAAGTATAGGGAGCTCCCCTGGGGTGGCCAGCAACCTGACAGAGCCAAGTTACT CAAGTAGTACCTGTGGAAGCCACACAGTACCTAGTCTTCATGCGGGGCTCCCATCTCAGGAATATGCTCC GGGATACAACGGCTCATATTTGCATTCTACCTACAGCAGCCAGGCCACACCTGCACTTCCTTCGCCTCAT CCGTCCCCGTTGCATAGTTCTGGGCTTTTGCAGCCACCACCCCCTCCTCCTCCCCCACCAGCCCTGGTCC CAGGCTACAACGGGACTTCCAATCTCTCCAGTTACAGTTATCCCTCTGCTAGCTATCCTCCTCAGACTGC CGTGGGGTCTGGGTACAGCCCTGGCGGAGCACCCCCTCCTCCTTCAGCATACCTGCCTTCAGGAATTCCT GCTCCCACCCCTCTGCCCCCCACTACCGTTCCTGGCTACACCTACCAAGGTCATGGTTTGACACCCATTG CACCCTCTGCTCTGACCAACAATTCAGCAAGTTCTCTCAAAAGGAAAGCTTTCTATATGGCAGGGCAAGG AGACATGGACTCCAGTTACGGAAATTACAGCTATGGCCAACAGAGATCGACACAGAGTCCTATGTACCGA ATGCCCGACAACAGCATTTCAAACTCGAATCGGGGGAATGGCTTTGACAGAAATGCTGAAACATCATCCT TAGCATTTAAGCCAACAAAGCAGCTGATGCCCTCTGAGCAGCAAAGGAAATTCAGCAGCCAGTCCAGTAG GGCTCTGACCCCTCCTTCCTATAGTACTGCTAAAAATTCATTGGGATCAAGGTCCAGTGAATCCTTTGGG AAGTATACGTCGCCAGTCATGAGTGAGCACGGAGACGACCATCGGCAGCTCCTCGCTCATCCAATACAAG GTCCCGGACTCCGTGCAGCTACCTCATCCAACCACTCTGTGGACGAGCAACTGAAGAATACTGACACACA CCTCATTGACCTGGTCACCAATGAGATCATCACCCAAGGACCTCCTGTGGACTGGAGCGACATAGCTGGT CTTGACCTGGTGAAGGCGGTTATTAAGGAGGAGGTTTTGTGGCCAGTGCTGAGGTCAGATGCATTCAGTG GGCTGACCGCCTTACCTAGAAGCATCCTTCTCTTCGGACCTCGGGGTACAGGCAAAACACTTTTGGGCAG ATGCATAGCTAGTCAGCTAGGGGCCACGTTTTTCAAAATTGCCGGTTCCGGACTCGTTGCAAAGTGGATA GGAGAAGCGGAGAAAATCATCCACGCCTCTTTTCTTGTGGCCAGGTGTCGCCAGCCCTCGGTGATTTTTG TTAGTGACATTGACATGCTTCTCTCCTCTCAAGTGAGCGAGGAACACAGTCCAGTCAGTCGGATGAGAAC CGAATTTCTGATGCAGCTGGACACTGTACTGACTTCGGCTGAGGACCAAATCGTAGTCATTTGTGCCACC AGTAAACCAGAAGAAATAGATGAATCTCTTCGGAGGTACTTCATGAAAAGACTTTTAATCCCACTTCCTG ACAGCACAGCGAGGCACCAGATAATAGTACAGCTGCTCACACAGCACAATTACTGTCTCAATGACAAGGA GTTTGCACTGCTTGTCCAGCGCACAGAAGGCTTTTCTGGACTAGACGTGGCTCATTTGTGTCAGGAAGCA GCAGTGGGACCTCTCCATGCTATGCCAGCTACAGACCTTTCAGCCATTATGCCCAGCCAGTTAAGGCCCG TTACATATCAAGACTTTGAAAATGCTTTCTGCAAGATTCAGCCTAGCATATCTCAAAAAGAGCTTGATAT GTATGTTGAATGGAACAAAATGTTTGGTTGCAGTCAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG212262 representing NM_021716
Red=Cloning site Green=Tags(s) MISSTSVYGLKMQWTPEHAQWPEQHFDITSTTRSPAHKVEAYRGHLQRTYQYAWANDDISALTASNLLKK YAEKYSGILEGPVDRPVLSNYSDTPSGLVNGRKNDSEPWQPSLNSEAVYPMNCVPDVITASKAGVSSALP PVDVSASIGSSPGVASNLTEPSYSSSTCGSHTVPSLHAGLPSQEYAPGYNGSYLHSTYSSQATPALPSPH PSPLHSSGLLQPPPPPPPPPALVPGYNGTSNLSSYSYPSASYPPQTAVGSGYSPGGAPPPPSAYLPSGIP APTPLPPTTVPGYTYQGHGLTPIAPSALTNNSASSLKRKAFYMAGQGDMDSSYGNYSYGQQRSTQSPMYR MPDNSISNSNRGNGFDRNAETSSLAFKPTKQLMPSEQQRKFSSQSSRALTPPSYSTAKNSLGSRSSESFG KYTSPVMSEHGDDHRQLLAHPIQGPGLRAATSSNHSVDEQLKNTDTHLIDLVTNEIITQGPPVDWSDIAG LDLVKAVIKEEVLWPVLRSDAFSGLTALPRSILLFGPRGTGKTLLGRCIASQLGATFFKIAGSGLVAKWI GEAEKIIHASFLVARCRQPSVIFVSDIDMLLSSQVSEEHSPVSRMRTEFLMQLDTVLTSAEDQIVVICAT SKPEEIDESLRRYFMKRLLIPLPDSTARHQIIVQLLTQHNYCLNDKEFALLVQRTEGFSGLDVAHLCQEA AVGPLHAMPATDLSAIMPSQLRPVTYQDFENAFCKIQPSISQKELDMYVEWNKMFGCSQ TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_021716 |
| ORF Size | 2277 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_021716.5, NP_068362.1 |
| RefSeq Size | 3875 bp |
| RefSeq ORF | 2280 bp |
| Locus ID | 60344 |
| UniProt ID | Q9ERZ6 |
| Gene Summary | ATP-dependent microtubule severing protein. Severs microtubules along their length and depolymerizes their ends, primarily the minus-end, suppressing microtubule growth from and attachment to centrosomes. Microtubule severing may promote rapid reorganization of cellular microtubule arrays and the release of microtubules from the centrosome following nucleation. Microtubule release from the mitotic spindle poles may allow depolymerization of the microtubule end proximal to the spindle pole, leading to poleward microtubule flux and poleward motion of chromosome (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC221381 | Fign (untagged) - Mouse fidgetin (Fign), (10ug) |
CNY 9,790.00 |
|
| MR212262 | Fign (Myc-DDK-tagged) - Mouse fidgetin (Fign) |
CNY 5,560.00 |
|
| MR212262L3 | Lenti ORF clone of Fign (Myc-DDK-tagged) - Mouse fidgetin (Fign) |
CNY 7,700.00 |
|
| MR212262L4 | Lenti ORF clone of Fign (mGFP-tagged) - Mouse fidgetin (Fign) |
CNY 7,700.00 |
