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Aak1 (NM_177762) Mouse Tagged ORF Clone
CAT＃: MG212422
- TrueORF®
Aak1 (tGFP-tagged) - Mouse AP2 associated kinase 1 (Aak1) transcript variant 2, (10ug)
ORF Plasmid: DDK
"NM_177762" in other vectors (4)
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CNY 9,600.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 5530400K14Rik; 9630042K20; AU067724; AU067726; BC028270; C79663; D6Ertd245e; mKIAA1048; R75501 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG212422 representing NM_177762
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGAAGTTTTTCGACTCCAGGCGGGAGCAGGGGAGCTCTGGCCTGGGTTCAGGCTCCAGCGGAGGAG GGGGCAGCAGCTCAGGCCTGGGCAGTGGTTACATCGGAAGGGTCTTCGGCATCGGGAGGCAGCAGGTCAC CGTGGACGAGGTGTTGGCGGAAGGTGGATTCGCTCTTGTCTTTCTGGTGAGAACAAGCAATGGGGTGAAA TGTGCCTTGAAACGTATGTTTGTCAACAATGAACATGACCTCCAGGTGTGCAAGAGGGAGATCCAGATCA TGAGAGACCTATCAGGGCACAAGAACATTGTGGGCTACATTGATTCTAGTATCAACAATGTGAGCAGCGG CGACGTCTGGGAAGTTCTCATTCTCATGGACTTCTGCAGAGGAGGCCAGGTGGTAAACCTGATGAACCAG CGTCTCCAGACAGGCTTCACAGAGAATGAAGTGCTGCAGATATTCTGTGACACCTGTGAAGCTGTGGCCC GCCTGCATCAGTGCAAAACTCCCATTATCCACCGGGACCTGAAGGTTGAAAATATCCTCTTGCATGACCG AGGCCACTATGTCTTGTGTGACTTTGGAAGTGCCACCAACAAATTCCAGAATCCACAGGCCGAGGGAGTC AATGCAGTAGAAGATGAGATTAAGAAATACACAACGCTCTCCTATCGAGCCCCAGAAATGGTCAACTTGT ACAGTGGCAAAATCATCACTACGAAGGCAGATATTTGGGCTCTAGGCTGTTTGTTGTATAAATTATGCTA CTTCACTTTGCCGTTTGGGGAGAGCCAGGTGGCGATTTGTGACGGAAGCTTCACAATTCCTGATAACTCT CGTTATTCTCAAGATATGCACTGCCTTATTAGGTATATGTTGGAACCAGACCCTGACAAAAGGCCGGATA TTTACCAGGTTTCATACTTCTCATTTAAACTACTCAAGAAAGAATGCCCCGTTCCAAATGTACAGAACTC TCCCATTCCTGCAAAACTTCCTGAACCAGTGAAAGCCAGTGAAGCAGCTGTGAAAAAGACCCAGCCAAAG GCCAGACTGACCGATCCCATTCCCACCACAGAGACTTCAATCGCACCCCGCCAGAGGCCTAAAGCTGGGC AGACTCAGCCAAACCCAGGCATCCTTCCCATCCAGCCAGCCCTGACTCCTCGGAAGAGGGCCACTGTTCA GCCCCTACCTCAGGCTGCAGGACCCAGCAATCAGCCTGGACTTCTACCCAGTGTTTCCCAACCTAAAGCC CAAGCCACACCCAGTCAACCTCTTCAGTCATCTCAACCCAAGCAACCACAAGCTCCACCCACCCCACAGC AGACACCTGCTACCCAGACACAAGGTTTGCCCACCCAGGCCCAGGCCACTCCCCAGCACCAGCAGCAACA CCTCCTCAAGCAACAGCAGCAACAGCAGCAACAGCCTCAGCAGCCTACAGCACCACCACAGCCTGCAGGC ACCTTTTACCAGCAGCAGCAGCAACAGCAGCAGCAGCAGGCTCAGACTCAGCAGCAGATTCAAGCCCCAG TGAGACAACAGCCAAAAGTTCAGACCACTCCACCTCCAACCATCCAGGGACAGAAAGTTGGATCTCTCAC TCCTCCATCATCACCCAAAACCCAACGTGCTGGGCACAGACGGATTCTCAGTGATGTAACCCACAGTGCA GTCTTTGGGGTTCCTGCCAGCAAATCAACCCAGCTGCTCCAGGCAGCTGCAGCTGAGGCCAGTCTCAATA AATCTAAGTCTGCAACCACCACTCCCTCAGGCTCTCCACGGACTTCACAGCAGAATGTCTCCAATGCTTC AGAAGGCTCCACATGGAATCCTTTTGATGACGACAACTTCTCCAAACTCACAGCTGAAGAGCTGCTAAAC AAGGACTTTGCCAAGCTTGGGGAAGGAAAGCTGCCTGAGAAGCTTGGTGGCTCTGCAGAGAGTTTGATCC CAGGCTTCCAGCCAACACAAGGAGATGCCTTCACCACTCCTTCTTTTTCTGCTGGAACTGCTGAAAAAAG GAAGGGGGGCCAGGCTGTGGACTCTGGCATCCCACTTCTAAGTGTGTCTGATCCCTTCATTCCTCTTCAA GTACCTGATGCTCCAGAAAAACTAATCGAGGGACTCAAATCTCCTGACACTTCTCTTCTGCTCCCTGACC TCTTGCCTATGACAGATCCTTTTGGCAGCACTTCTGATGCTGTTATTGACAAAGCTGATGTTGCTGTTGA GAGTCTCATACCTGGACTGGAGCCACCTGTTGCTCAGCGCCTCCCATCTCAAACGGAATCTGTGACCTCG AACCGCACAGACTCTCTCACGGGGGAAGACTCTCTGCTTGACTGCTCTCTGCTTTCTAACCCTACTGCTG GCCTTCTGGAGGAGTTTGCCCCCATAGCACTCTCTGCTCCCACTCATAAAGCTGCAGAAGATAGCAATCT CATCTCAGGTTTTGGTGTTGCTGAGGGCTCCGAAAAGGTGGCAGAAGATGAGTTTGACCCTATTCCTGTA CTAATTACCAAAAACACACAAGGTGGGCACTCTAGGAACAGCAGTGGGAGCTCTGAGTCCAGTCTTCCCA ACCTAGCCAGGTCTTTGCTGCTGGTGGATCAGCTCATAGACCTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG212422 representing NM_177762
Red=Cloning site Green=Tags(s) MKKFFDSRREQGSSGLGSGSSGGGGSSSGLGSGYIGRVFGIGRQQVTVDEVLAEGGFALVFLVRTSNGVK CALKRMFVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINNVSSGDVWEVLILMDFCRGGQVVNLMNQ RLQTGFTENEVLQIFCDTCEAVARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKFQNPQAEGV NAVEDEIKKYTTLSYRAPEMVNLYSGKIITTKADIWALGCLLYKLCYFTLPFGESQVAICDGSFTIPDNS RYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLLKKECPVPNVQNSPIPAKLPEPVKASEAAVKKTQPK ARLTDPIPTTETSIAPRQRPKAGQTQPNPGILPIQPALTPRKRATVQPLPQAAGPSNQPGLLPSVSQPKA QATPSQPLQSSQPKQPQAPPTPQQTPATQTQGLPTQAQATPQHQQQHLLKQQQQQQQQPQQPTAPPQPAG TFYQQQQQQQQQQAQTQQQIQAPVRQQPKVQTTPPPTIQGQKVGSLTPPSSPKTQRAGHRRILSDVTHSA VFGVPASKSTQLLQAAAAEASLNKSKSATTTPSGSPRTSQQNVSNASEGSTWNPFDDDNFSKLTAEELLN KDFAKLGEGKLPEKLGGSAESLIPGFQPTQGDAFTTPSFSAGTAEKRKGGQAVDSGIPLLSVSDPFIPLQ VPDAPEKLIEGLKSPDTSLLLPDLLPMTDPFGSTSDAVIDKADVAVESLIPGLEPPVAQRLPSQTESVTS NRTDSLTGEDSLLDCSLLSNPTAGLLEEFAPIALSAPTHKAAEDSNLISGFGVAEGSEKVAEDEFDPIPV LITKNTQGGHSRNSSGSSESSLPNLARSLLLVDQLIDL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_177762 |
| ORF Size | 2634 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_177762.7 |
| RefSeq Size | 19120 bp |
| RefSeq ORF | 2637 bp |
| Locus ID | 269774 |
| UniProt ID | Q3UHJ0 |
| Gene Summary | Regulates clathrin-mediated endocytosis by phosphorylating the AP2M1/mu2 subunit of the adaptor protein complex 2 (AP-2) which ensures high affinity binding of AP-2 to cargo membrane proteins during the initial stages of endocytosis. Isoform 1 and isoform 2 display similar levels of kinase activity towards AP2M1. Regulates phosphorylation of other AP-2 subunits as well as AP-2 localization and AP-2-mediated internalization of ligand complexes. Phosphorylates NUMB and regulates its cellular localization, promoting NUMB localization to endosomes. Binds to and stabilizes the activated form of NOTCH1, increases its localization in endosomes and regulates its transcriptional activity (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC222351 | Aak1 (untagged) - Mouse AP2 associated kinase 1 (Aak1), transcript variant 2, (10ug) |
CNY 11,210.00 |
|
| MR212422 | Aak1 (Myc-DDK-tagged) - Mouse AP2 associated kinase 1 (Aak1), transcript variant 2 |
CNY 8,740.00 |
|
| MR212422L3 | Lenti ORF clone of Aak1 (Myc-DDK-tagged) - Mouse AP2 associated kinase 1 (Aak1), transcript variant 2 |
CNY 10,640.00 |
|
| MR212422L4 | Lenti ORF clone of Aak1 (mGFP-tagged) - Mouse AP2 associated kinase 1 (Aak1), transcript variant 2 |
CNY 10,640.00 |
