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Ppl (NM_008909) Mouse Tagged ORF Clone
CAT＃: MG215229
- TrueORF®
Ppl (tGFP-tagged) - Mouse periplakin (Ppl), (10ug)
ORF Plasmid: DDK
"NM_008909" in other vectors (2)
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CNY 19,190.00
货期*
3周
规格
Product images
经常一起买 (3)
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AW553870 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG215229 representing NM_008909
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCACTCGCTCTTCAGGAAGAGAAACAAAGGCAAATACAGCCCAACGGTGCAGACGCGGAGCATCTCCA ACAAGGAACTCTCAGATCTGATTGAGCAGCTGCAGAAGAATGCAGACCAGGTGGAAAGGAACATTGTGGA CACCGAGGCCAAGATGCAGAGCGACTTGGCCCGGATGCAAGAGGGGCAGCTCCCTGAGCACCGGGACGCG GCCCTGCAGAACGTGTCTGACTCAGAAAAGCTGCTGTACGTGCTGGAGGCAGATTCGGCCATCGCCAAGC ACATGAAGCACCCACAGGGGGACATGATCGCCGAGGACATCCGCCAGCTGAAAGAGCGCGTGACCAACCT GCGGGGGAAACACAAGCAGATGTACAGCCTGGCAGTGAAAGAGGCCGACCCGAGAGTCAACTGGGACACA CTGGTGGACGAGAAGCTGGACAAGCTGAGCAGCCAGAGCTTTGGGACTGACCTTCCTCTGGTGGATAGCC AGGTGGAGCAACACAACATCTTCCACAATGAAGTCAAAGCCATCGGGCCCCACCTGGCCAAGGACAAGGA GCAGAACAGTGAACTCCAAGCCAAGTATCAGAAGTTGCTGACAGCATCACAGGCACGGCAGCAGCACCTG AGCTCGCTGCAGGATTACATGCAGCGCTGCACCAATGAGCTGTACTGGCTGGACCAGCAAGCCAAGGGCC GCATGCAGTATGACTGGAGCGACCGCAACCTCGACTACCCCAGCCGCCGCCGCCAGTACGAGAATTTCAT CAACCGGAACCTGGAGGCCAAAGAAGAAAGAATCAACAAACTGCACACTGAAGGTGACCAACTGCTGACA GCTGAGCACCCAGGGAGGAACTCCATTGAGGCACACATGGAGGCTGTGCATGCTGAGTGGAAGGAGTACC TGAACCTGCTCATCTGTGAGGAGAGCCATCTGAAATACATGGAAGACTACCACCAATTTCACAAAGACAT GAAGGATGCTCAGGAGCTGCTACGCAAGGTGGACTCAGACTTGAACCAGAAATATAGCCCTGACTTCAAA GACCGGTATCAGATAGAGCTGTTGCTTCGGGAACTGGATGACCAGGAGAAGGCTCTGGACAAGTATGAGG ATGTGGTGCGAGGGCTGCAGAGGCGAGGGCAGCAAGTGGTGCCACTCAAGTACCGCCGCGAGACGCCGCT CAAGCCCATCCCTGTGGAGGCTCTCTGTGACTTTGAGAGTGACCAGGGCCTCATCTCGCGGGGCTATAGC TACACACTGCAGAAGAACAACGGGGAGAGCTGGGAGCTGACAGACAGCACTGGGAAGAAGCTGGCTGCCC CAGCCGTCTGCTTCATCATTCCCCCCACCGACCCCGAGGCCTTGGCTCTTGCTGACAGCCTGGGCAGCCA GTACCGAAGTGTGAGGCAGAAGGCGACTGGGAGCAAACATGCACTCCAGCAGCGGCATGAGGTGCTAAGG ACGGAGAACCCTGGAGATGCCTCTGATCTTCAGGGACGACAGCTGCTGGCTGGCTTGGACAAAGTGGCCA GTGACCTGGACCGGCAGGAAAAGGCTATCACAGGGATCCTACGGCCACCACTTGAGCAGGGACGGGCAAT TGAGGATAGTGCGGAGCGGGCCAAGGGTCTCAAGAACATTACCAATGAGCTGCTACAGATTGAGCCAGAG AAGACGCAGTGCACAGCCGAGTGTGAAGCCTTTGTGCAGGCCCTCCCGGCCAGCGGCACCGCACCCTTGC TGAAGACCCGGGTAGAAGACACCAACCAGAAATATGAGCGACTAGTATGGTTGCTAGAGGCAGCCCAGGA GAAGGTGGACGTGGCCAACCGCCTGGAGAACAGTCTGCAGCGGGGCAGGGAGCTGCTGGCCTCATACGAG AACAGGCTGATTCAGGACGACACAATGCCTGAAAGTGGTCATGTGTTGGACAGCAAGAGGCAGGAGCTGG AGGCCATGGCTTCTGAGCTGCAGGCCCACAAGTCCCTCCTGGGTGAGGTGGAACAGAACCTACAGGTGGC CAAGCAGTGTTCCAGCTCACTGGCCAGCCGCTTCCAAGAGCACTGCCCTGACCTGGAGCGGCAGGAGGCC GAGGTGCACAAGCTCAACCAACGTTTCAACAACCTCAGCCAGCAGGTGGAACGGAGGGCCCAGAGCCTGC AGAGTGCCAGGGCTGCCTACGATGAATACTGCAGTGGTTACAACCGTGTGCTCCAGTTTTTGGCCAAGAC TCCCAGTTATGAGCCCCAGGAGACAGACAGCCTCGGCCAGATGGAGACCAAGCTCAAGAACCAGAAGAAC TTGCTTGATGAGCTAGCAAGCAGGGAACAGGAGGTGCAGAAGGTCTATGCAGATTCCCAGCAGTACCAGC AAGCGGTGAAGGACTATGAACTTGAAGCAGAGAAGCTAAGGTCCTTGCTGGACCTGGAGAATGGACGGAA CAGCCATGTGAACAAGAGAGCCAGGCTGCAGTCTCCTGCTGCCAAAGTGAAGGAAGAGGAAGCTGCTCTT GCTGCCAAGTTCACAGAGGTTAATGCCATCAACAGACAGAGGCTACAGAATTTGGAGTTTGCACTGAATC TCTTGAGACAGCAACCAGAAGCAGGAGTGACCCATGAGACCCTACAAGGGGGAAAGCTAAGCTCTGGCAT GGAGGAAACATGGAAGATTAAGAAGGAACTGGAGGAGGAGATAGAGCGAAGGCAACAGCTGGAAAATGAG GTCAAGAGTGCCCAGGAGGAAATCCAGACCCTGAAGGATCAGGGGCCTCAGGAGTCATTGGTGAGGAAGG AGGTGCTGAAGAAGGTGCCAGACCCTGCCCTGGAGGAGAGCTTCCAGCAGCTGCAGCAGACGCTGGCTGA GGAACAGCACAAGAACCAGCTATTGCAGGAGGAGCTGGGAGCACTGCAGCTCCGGCTGCAGGCTCTAGAG CAGGAGACCAGGGATGGGGGACAGGAGTATGTGGTCAAGGAGGTCTTGCGCATTGAGCCAGACAGAGCCC AGGAGGATGAGGTCTTGCAGCTTCGGGAGGAGCTGGAGGCACTGCGGCGCCAGAAAGGCGCCCGAGAGGC TGAAGTGCTACTCTTACAGCAGCGTGTAGCAGCCCTGGCTGCTGAGAAGAGCAGGGTGCAGGAAAAGGTC ACTGAGAGAGAGGTGGTAAAGCTACAGAATGACCCCCAACTGGAGGCAGAATACCGGAGGCTGCAGGAGG AGCACCAGCGGGAGGGCACACTCAGGGAGAAGCAGGAGGAGGAGCTGAGTTTCCTACAGGCTAAGCTCAG GAGACTGGAGAAAGAACGAGCCATGGCAGAAGGCAAGATCACTGTCAAAGAGGTGCTCAAGGTAGAGAAA GATGCAGCTGTGGAGAGAGAAGTCAATGACCTCACCCGCCAGTATGAGGACGAGGCTGCCAAGGCACGCT CTGGCCAGCGGGAGAAGACAGAACTTCTCAGAAAGATATGGGCCCTGGAAGAGGAAAATGCTAAGGTGGT GGTACAGGAGAAGGTCCGAGAGATCGTGCGGCCAGATCCCAAGGCAGAGAGTGAAGTGGCCAACCTCCGC CTGGAGCTGGTGGAGCAGGAGCGCAAATTCAGGGGTGCTGAGGAGCAGCTGAAGAGCTACCAGAGTGAGC TGGAGGCCCTACGGAACCGTGGACCACAGGTAGAAGTCAAAGAGGTGACCAAAGAGGTCATTAAATATAC AACTGACCCAGAGACAGAGCAGGAACTCCAGAGGCTCAGGGAGGAGATCATGGACAAGACCAGGTTAATA GAAAGGTGTGACCTAGAGATTTACCAGCTGAAGCAGGAGATCCAGGCCCTTAAGGACACCAAGCCACAGG TGCAGACTAGAGAGGTAGTCCAGGAGATCCTGCAGTTCCAGGAAGACCCCCAAACCAAGAAGGAGGTAGA GTCTCTGCGTATACAGCTGTCAGAAGAACAGAAGAAACAGGTGGACCTGGAAGGGGAGCGGGCATCCCAG GAAGAGAAGATCAAACGCAAGGAGGAAGAGCTGGCACAGGGGAAGGAAAGGGTTGTACGCCAGGAGGTAG TACAGTATGAAGATGAGCCAGATTTGCGAGCTGAGGTGACTGCCTTCACAAACAGCATTGATGCTGAGCT GCGGCAGATTGATAAACTGCATGTGGAGCTACGGCGGTTACAGCATCGCCGAGCAGAGCTGGAGAGGCAG TTGGAGGAGCTGGAGCGGGAGAGGCAGGCACGTAGGGCAGCCGAGCTGGAGGTACAGAGGCTGCAACAGC GGCTGGCTGCATTGGAGCAGGAGGAGGCCAAGACTGGTGAGAAGGTGACCCACACACAAAAGGTGGTGCT GCAGCAGGACCCACAGCAGACCAGGGAGCATGCCCTGCTCCGAGCCCAGCTGGAGGAAGAGCGGCACCGG AGGCAGCTACTGGAGGGTGAGCTTGAGCCGCTGCGTAGAAAGTTGGCTGCCCTGGAGAAGGCAGAGATCA AAGAAAAGGTGGTCTTCTCTGAGAGTGTCCAGGTAGAAAAAGGTGACACTGAGCAAGAGATCCAGCGCCT CAAGAAGAGCCTGGAAGAGGAGAGCCAGAGCAAGAGGGAGCTGGATTCAGAGGTGACCCGGCTAGAAGCC AAGTTATCTGAGCTAGAATTCTATAATTCCAAGTCATCCAAGGAACTGGACTTCCTGAGAGAAGAAAACC ACAAGCTACAGCTAGAGCGGCAAAACCTGCAGCTAGAGACCCGGAGACTCCAATCAGAGATTGAGATGGC AGCAACAGAAACTCGAGATCTGAAGAACATAACCACGATAGACTCTGGGACCCACCTAAACTCTAGGCTG TGGTCCCTAGAGAAGGAACTGGATGACCTCAAGAAGATGTCCAAGGACAAGGATCTGGAGATCGATGAAC TACAGAGGCGCCTGGGCTCTGTGGCTGTCAAGAGAGAGCAGAGGGAGAACCACCTGAGGCGCTCCATAGT GGTCATAGACCCTGACACAGGTCGTGAGCTGTCCCCAGAGGAGGCTCACCGGGCTGGGCTTATCGACTGG AAAATGTTTGTGAAGCTCAGAAGCCAGGAGTGCGACTGGGAGGAGATATCAGTGAAAGGTCCTAATGGGG AGTCATCAGTAATTCATGACAGGAAGTCAGGCAAGAAGTTCTCCATTGAGGACGCTCTTCAGAGTGGCAG GCTAACCGCTGCTCAATATGACCGCTATGTCAACAAGGATATGTCCATTCAGGAGCTGGCAGTCTTGGTG TCTGGGCAGAAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG215229 representing NM_008909
Red=Cloning site Green=Tags(s) MHSLFRKRNKGKYSPTVQTRSISNKELSDLIEQLQKNADQVERNIVDTEAKMQSDLARMQEGQLPEHRDA ALQNVSDSEKLLYVLEADSAIAKHMKHPQGDMIAEDIRQLKERVTNLRGKHKQMYSLAVKEADPRVNWDT LVDEKLDKLSSQSFGTDLPLVDSQVEQHNIFHNEVKAIGPHLAKDKEQNSELQAKYQKLLTASQARQQHL SSLQDYMQRCTNELYWLDQQAKGRMQYDWSDRNLDYPSRRRQYENFINRNLEAKEERINKLHTEGDQLLT AEHPGRNSIEAHMEAVHAEWKEYLNLLICEESHLKYMEDYHQFHKDMKDAQELLRKVDSDLNQKYSPDFK DRYQIELLLRELDDQEKALDKYEDVVRGLQRRGQQVVPLKYRRETPLKPIPVEALCDFESDQGLISRGYS YTLQKNNGESWELTDSTGKKLAAPAVCFIIPPTDPEALALADSLGSQYRSVRQKATGSKHALQQRHEVLR TENPGDASDLQGRQLLAGLDKVASDLDRQEKAITGILRPPLEQGRAIEDSAERAKGLKNITNELLQIEPE KTQCTAECEAFVQALPASGTAPLLKTRVEDTNQKYERLVWLLEAAQEKVDVANRLENSLQRGRELLASYE NRLIQDDTMPESGHVLDSKRQELEAMASELQAHKSLLGEVEQNLQVAKQCSSSLASRFQEHCPDLERQEA EVHKLNQRFNNLSQQVERRAQSLQSARAAYDEYCSGYNRVLQFLAKTPSYEPQETDSLGQMETKLKNQKN LLDELASREQEVQKVYADSQQYQQAVKDYELEAEKLRSLLDLENGRNSHVNKRARLQSPAAKVKEEEAAL AAKFTEVNAINRQRLQNLEFALNLLRQQPEAGVTHETLQGGKLSSGMEETWKIKKELEEEIERRQQLENE VKSAQEEIQTLKDQGPQESLVRKEVLKKVPDPALEESFQQLQQTLAEEQHKNQLLQEELGALQLRLQALE QETRDGGQEYVVKEVLRIEPDRAQEDEVLQLREELEALRRQKGAREAEVLLLQQRVAALAAEKSRVQEKV TEREVVKLQNDPQLEAEYRRLQEEHQREGTLREKQEEELSFLQAKLRRLEKERAMAEGKITVKEVLKVEK DAAVEREVNDLTRQYEDEAAKARSGQREKTELLRKIWALEEENAKVVVQEKVREIVRPDPKAESEVANLR LELVEQERKFRGAEEQLKSYQSELEALRNRGPQVEVKEVTKEVIKYTTDPETEQELQRLREEIMDKTRLI ERCDLEIYQLKQEIQALKDTKPQVQTREVVQEILQFQEDPQTKKEVESLRIQLSEEQKKQVDLEGERASQ EEKIKRKEEELAQGKERVVRQEVVQYEDEPDLRAEVTAFTNSIDAELRQIDKLHVELRRLQHRRAELERQ LEELERERQARRAAELEVQRLQQRLAALEQEEAKTGEKVTHTQKVVLQQDPQQTREHALLRAQLEEERHR RQLLEGELEPLRRKLAALEKAEIKEKVVFSESVQVEKGDTEQEIQRLKKSLEEESQSKRELDSEVTRLEA KLSELEFYNSKSSKELDFLREENHKLQLERQNLQLETRRLQSEIEMAATETRDLKNITTIDSGTHLNSRL WSLEKELDDLKKMSKDKDLEIDELQRRLGSVAVKREQRENHLRRSIVVIDPDTGRELSPEEAHRAGLIDW KMFVKLRSQECDWEEISVKGPNGESSVIHDRKSGKKFSIEDALQSGRLTAAQYDRYVNKDMSIQELAVLV SGQK TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_008909 |
| ORF Size | 5262 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_008909.2, NP_032935.2 |
| RefSeq Size | 6277 bp |
| RefSeq ORF | 5265 bp |
| Locus ID | 19041 |
| UniProt ID | Q9R269 |
| Gene Summary | Component of the cornified envelope of keratinocytes. May link the cornified envelope to desmosomes and intermediate filaments. May act as a localization signal in PKB/AKT-mediated signaling.[UniProtKB/Swiss-Prot Function] |
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