Adar (NM_001146296) Mouse Tagged ORF Clone
CAT#: MG215879
- TrueORF®
Adar (tGFP-tagged) - Mouse adenosine deaminase RNA-specific (Adar) transcript variant 3, (10ug)
ORF Plasmid: DDK
"NM_001146296" in other vectors (3)
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CNY 11,400.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | Adar1; Adar1p110; Adar1p150; AV242451; mZaADAR |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG215879 representing NM_001146296
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTCAAGGGTTCAGGGGACCCACAGGGGTGTTCCCTCACCAGACACAGTCGTACTTGGACCCTAGTC ATGAGCATAGCAAGTGGAGATACCCGCAGCCACAGGGGCCGGAGTCTTACCCTAGGAGTTTCCAGCTTCA GCAGATAGAGTTTCTCAAAGGGCGGCTCCCAGAAGCACCCTTGATTGGAATACAAACCCAGTCACTGCCG CCATTCCTCCCAGGACACTGGCCAAGATTCCCAGGGCCACCTGCCCAAGACAGGCAACTGGAAATCTGGG AGTTCCCCAGGAGTGTGACTCTCAGAAATCAGGGGTTCCACATAGGACCCCCACTTCCTCCCCCACACAG CAGGGGTACACCATGGAGAGGTGCTGACGGGCTTTGCTCACACTTCCGGGAGCTGAGCATCAGTCAGAGT CCGGAGCAGAAGGTTCTAAACCGCCTGGAAGAGCTTGGGGAGGGGAAGGCCACCACTGCCCATGTGCTAG CCAGAGAGCTCAGAATCCCCAAAAGGGACATCAATCGTATTTTGTACTCCCTGGAAAAGAAGGGAAAGCT GCACAGAGGAAGGGGGAAACCTCCTTTGTGGAGCCTTGTGCCCTTGAGTCAGGCTTGGACTCAGCCCCCT GGAGTTGTGAATCCAGATAGTTGTATCCAGGAATTCCCTAGAGGAGAGCCTGGTTTGGACAGTGAGGACG GAGACCCTGCCTCTGACTTAGAAGGACCTTCTGAGCCTCTTGACATGGCTGAAATCAAGGAGAAGATCTG TGACTATCTGTTCAATGTGTCAAACTCCTCTGCCCTGAACCTGGCTAAGAACATTGGCCTCACCAAGGCC CGAGATGTGACCTCAGTGCTGATTGACTTGGAAAGGCAAGGCGATGTCTACAGGCAAGGGGCAACTCCTC CCATCTGGTACTTGACGGACAAGAAGCGTGAGAGGCTGCAGATGAAGAGAAGTACACACAGTGCTCCTGC CCCTACCCCGACAGCTGTCCCAGAGGCCACTAGAAGCCCCTCATTCCCTGCCTGCCACCCGCCCCCAGCA GGTGCCTCAAGCAGTGTGGCAGCCTCCAAGAGAGTGGAGAATGGGCAGGAGCCTGCGATAAAGCATGAAA GTAGGCATGAGGCCAGACCAGGACCAATGAGACTGCGGCCTCACGCTTATCACAATGGCCCCTCTAGAGC AGGGTATGTGGCCTCTGAAAATGGCCAGTGGGCCACAGATGACATCCCAGATAACTTGAATAGTATCCAC ACAGCACCAGGTGAGTTTCGAGCCATCATGGAGATGCCCTCCTTCTACAGCCCTACCTTGCCACGGTGTT CACCCTACAAGAAGCTAACTGAGTGCCAGCTGAAGAACCCTGTCAGCGGGTTGTTAGAGTATGCTCAGTT CACTAGTCAGACCTGTGATTTCAACCTGATAGAGCAGAGTGGACCGTCCCATGAACCTCGATTTAAATTC CAGGTTGTCATCAATGGGCGGGAATTTCCCCCAGCTGAGGCTGGCAGCAAGAAAGTAGCCAAGCAGGACG CAGCAGTGAAAGCCATGGCGATTCTGCTTCGGGAAGCCAAAGCCAAAGACAGTGGTCAACCAGAAGACTT GTCCCACTGTCCCATGGAAGAAGACTCGGAGAAACCAGCAGAGGCTCAGGCCCCCAGCTCCTCAGCAACA TCCTTGTTCTCTGGGAAGAGCCCAGTTACTACACTGCTTGAGTGCATGCACAAACTAGGGAACTCCTGTG AATTCCGTCTCCTGTCCAAAGAAGGCCCTGCTCATGACCCCAAGTTCCAGTACTGTGTAGCAGTAGGAGC CCAGACCTTCCCCCCTGTGAGCGCCCCCAGCAAGAAGGTAGCAAAGCAGATGGCCGCAGAGGAAGCCATG AAGGCGCTGCAGGAGGAGGCAGCCAGTTCAGCGGATGACCAGTCTGGAGGTGCGAACACAGACTCACTTG ATGAATCTATGGCTCCCAACAAGATCAGGAGGATTGGTGAGCTCGTCAGGTACCTGAACACCAACCCCGT AGGCGGCTTGTTGGAGTACGCCCGATCTCATGGCTTTGCTGCTGAGTTCAAGCTCATTGACCAGTCTGGA CCTCCTCACGAACCCAAGTTTGTTTACCAAGCAAAAGTTGGGGGCCGCTGGTTTCCAGCCGTGTGTGCAC ACAGCAAGAAACAGGGCAAGCAGGATGCAGCGGATGCAGCCCTCCGGGTCTTGATCGGGGAGAGCGAGAA GGCAGAGCAGTTGGGTTTCGCAGAGGTAACCCCAGTAACAGGGGCCAGTCTCAGAAGAACTATGCTCCTC CTTTCCAGGTCCCCAGATGCACATCCAAAGACACTTCCTCTCTCTGGCAGCACCTTCCACGACCAGATAG CTATGCTGAGCCACAGGTGCTTCAATGCTCTGACCAACAGTTTCCAGCCCTCCCTGCTCGGCCGCAAGAT CCTGGCTGCCATTATTATGAAAAGAGATCCAGAGGACATGGGTGTTGTCGTGAGTTTGGGGACAGGCAAT CGCTGTGTGAAAGGGGACTCTCTCAGCCTGAAGGGAGAGACGGTCAATGACTGCCATGCCGAAATCATCT CCCGGAGGGGCTTCATCAGGTTTCTCTACAGTGAACTGATGAAGTACAACCACCACACTGCCAAGAACAG CATATTTGAGCTTGCCAGGGGAGGAGAGAAGCTGCAGATAAAGAAGACGGTTTCTTTTCATCTCTACATC AGCACGGCGCCATGTGGAGATGGGGCCCTCTTTGACAAATCCTGCAGTGACCGTGCCGTGGAAAGCACAG AGTCCCGCCATTACCCTGTCTTTGAAAATCCCAAGCAAGGCAAGCTTCGCACCAAGGTGGAGAATGGGGA AGGCACAATTCCTGTGGAGTCCAGTGATATTGTACCCACGTGGGATGGCATCCGCCTTGGGGAAAGACTC CGTACCATGTCCTGTAGTGACAAAATCCTACGCTGGAATGTGCTGGGCCTGCAAGGGGCGTTGTTGACGC ACTTCCTACAGCCTGTGTACCTGAAATCTGTAACCTTAGGTTACCTTTTCAGCCAAGGGCATCTGACCCG TGCTATTTGCTGCCGCGTGACCAGAGATGGGAAAGCATTTGAGGATGGACTACGCTATCCCTTTATTGTC AACCACCCCAAGGTCGGCCGAGTCAGTGTTTATGATTCCAAAAGACAGTCCGGAAAGACCAAGGAAACAA GCGTCAACTGGTGCATGGCTGATGGCTATGACCTAGAGATCCTGGATGGCACCAGAGGCACTGTGGATGG ACCAGGGAAAGAGTTGTCTCGGGTGTCCAAGAAGAATATTTTCCTTCAGTTTAAGAAGCTCTGCTCCTTC CGAGCCCGCAGAGATTTACTGCAGCTCTCTTATGGTGAAGCCAAGAAAGCTGCCCGTGACTATGACTTAG CCAAGAACTACTTCAAGAAAAGCCTGCGAGACATGGGCTATGGGAATTGGATCAGCAAACCCCAGGAGGA AAAGAACTTTTACCTCTGTCCAGTACCCAATGAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG215879 representing NM_001146296
Red=Cloning site Green=Tags(s) MSQGFRGPTGVFPHQTQSYLDPSHEHSKWRYPQPQGPESYPRSFQLQQIEFLKGRLPEAPLIGIQTQSLP PFLPGHWPRFPGPPAQDRQLEIWEFPRSVTLRNQGFHIGPPLPPPHSRGTPWRGADGLCSHFRELSISQS PEQKVLNRLEELGEGKATTAHVLARELRIPKRDINRILYSLEKKGKLHRGRGKPPLWSLVPLSQAWTQPP GVVNPDSCIQEFPRGEPGLDSEDGDPASDLEGPSEPLDMAEIKEKICDYLFNVSNSSALNLAKNIGLTKA RDVTSVLIDLERQGDVYRQGATPPIWYLTDKKRERLQMKRSTHSAPAPTPTAVPEATRSPSFPACHPPPA GASSSVAASKRVENGQEPAIKHESRHEARPGPMRLRPHAYHNGPSRAGYVASENGQWATDDIPDNLNSIH TAPGEFRAIMEMPSFYSPTLPRCSPYKKLTECQLKNPVSGLLEYAQFTSQTCDFNLIEQSGPSHEPRFKF QVVINGREFPPAEAGSKKVAKQDAAVKAMAILLREAKAKDSGQPEDLSHCPMEEDSEKPAEAQAPSSSAT SLFSGKSPVTTLLECMHKLGNSCEFRLLSKEGPAHDPKFQYCVAVGAQTFPPVSAPSKKVAKQMAAEEAM KALQEEAASSADDQSGGANTDSLDESMAPNKIRRIGELVRYLNTNPVGGLLEYARSHGFAAEFKLIDQSG PPHEPKFVYQAKVGGRWFPAVCAHSKKQGKQDAADAALRVLIGESEKAEQLGFAEVTPVTGASLRRTMLL LSRSPDAHPKTLPLSGSTFHDQIAMLSHRCFNALTNSFQPSLLGRKILAAIIMKRDPEDMGVVVSLGTGN RCVKGDSLSLKGETVNDCHAEIISRRGFIRFLYSELMKYNHHTAKNSIFELARGGEKLQIKKTVSFHLYI STAPCGDGALFDKSCSDRAVESTESRHYPVFENPKQGKLRTKVENGEGTIPVESSDIVPTWDGIRLGERL RTMSCSDKILRWNVLGLQGALLTHFLQPVYLKSVTLGYLFSQGHLTRAICCRVTRDGKAFEDGLRYPFIV NHPKVGRVSVYDSKRQSGKTKETSVNWCMADGYDLEILDGTRGTVDGPGKELSRVSKKNIFLQFKKLCSF RARRDLLQLSYGEAKKAARDYDLAKNYFKKSLRDMGYGNWISKPQEEKNFYLCPVPND TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001146296 |
ORF Size | 3534 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001146296.1, NP_001139768.1 |
RefSeq Size | 5928 bp |
RefSeq ORF | 3537 bp |
Locus ID | 56417 |
UniProt ID | Q99MU3 |
Gene Summary | Catalyzes the hydrolytic deamination of adenosine to inosine in double-stranded RNA (dsRNA) referred to as A-to-I RNA editing. This may affect gene expression and function in a number of ways that include mRNA translation by changing codons and hence the amino acid sequence of proteins; pre-mRNA splicing by altering splice site recognition sequences; RNA stability by changing sequences involved in nuclease recognition; genetic stability in the case of RNA virus genomes by changing sequences during viral RNA replication; and RNA structure-dependent activities such as microRNA production or targeting or protein-RNA interactions. Can edit both viral and cellular RNAs and can edit RNAs at multiple sites (hyper-editing) or at specific sites (site-specific editing). Its cellular RNA substrates include: bladder cancer-associated protein (BLCAP), neurotransmitter receptors for glutamate (GRIA2) and serotonin (HTR2C) and GABA receptor (GABRA3). Site-specific RNA editing of transcripts encoding these proteins results in amino acid substitutions which consequently alters their functional activities. Exhibits low-level editing at the GRIA2 Q/R site, but edits efficiently at the R/G site and HOTSPOT1. Does not affect polyomavirus replication but provides protection against virus-induced cytopathic effects. Essential for embryonic development and cell survival and plays a critical role in the maintenance of hematopoietic stem cells.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MR215879 | Adar (Myc-DDK-tagged) - Mouse adenosine deaminase, RNA-specific (Adar), transcript variant 3 |
CNY 9,024.00 |
|
MR215879L3 | Lenti ORF clone of Adar (Myc-DDK-tagged) - Mouse adenosine deaminase, RNA-specific (Adar), transcript variant 3 |
CNY 12,260.00 |
|
MR215879L4 | Lenti ORF clone of Adar (mGFP-tagged) - Mouse adenosine deaminase, RNA-specific (Adar), transcript variant 3 |
CNY 12,260.00 |