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Lonp1 (NM_028782) Mouse Tagged ORF Clone
CAT＃: MG215882
- TrueORF®
Lonp1 (tGFP-tagged) - Mouse lon peptidase 1 mitochondrial (Lonp1) nuclear gene encoding mitochondrial protein, (10ug)
ORF Plasmid: DDK
"NM_028782" in other vectors (4)
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CNY 7,890.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 1200017E13Rik; LON; Prss15 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG215882 representing NM_028782
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCGAGCACAGGCTACGTGCGGCTCTGGGCCGCTGCGCGGTGCTGGGTTCTGCGGCGGCCGCTGC TGGCTGTCACCGGAGGCCGGGTCCCGAGCGCGTCCGGGTCGTGGCTGCGCCGAGGATGTCGGGCCTGCGA CATGTCAGCTCCCTGGGGCGGCCGCGTTCTTCCCGGCGGGGTCCAGTGGCGGGGGCTGTGGGACTCGGGC AACCGCGGTGGCAGCGACGAGACCTCCGAGGGCGGTGCGGAGGACGGGGCCACGGCGAGCACCGGGGAAG GCCCGGTCGTCACAGCGCTCGCGCCCATGACCGTCCCGGATGTGTTTCCTCACCTGCCGCTCATCGCCAT CACCCGCAACCCTGTGTTCCCGCGCTTTATCAAGATCGTGGAGGTTAAAAATAAGAAGTTGGTTGAGCTC CTGAGAAGGAAAGTCCGACTTGCACAGCCCTATGTTGGCGTCTTCCTAAAGAGAGATGACAACAATGAGT CGGACGTGGTGGAGAGCCTGGATGAAATCTACCATACGGGAACATTCGCTCAGATTCATGAGATGCAGGA CCTCGGAGACAAGTTGCGAATGATTGTCACTGGCCACAGAAGGATTCACATCAGCCGGCAGCTGGAGGTG GAACCAGAGGGGCTTGAGCCAGAGGCAGAGAAACAAAAGTCCAGGAGGAAGCTGAAGCGAGGCAAGAAAG AGGTGGAGGATGAGCTGGGCCCCAAGCCCCAGCTGGAGATGGTGACCGAGGCTGCCACTGACACATCCAA GGAGGTGCTCATGGTGGAGGTTGAGAATGTAGCCCATGAAGACTTCCAGGTCACAGAGGAAGTGAAGGCC CTGACTGCAGAGATTGTGAAGACCATTCGGGATATCATCGCCTTGAACCCTCTGTACAGAGAGTCCGTGC TGCAGATGATGCAGGCAGGCCAGCGTGTGGTGGACAACCCCATCTACTTGAGCGACATGGGTGCGGCACT CACTGGGGCGGAGTCCCATGAGCTGCAAGATGTTCTGGAAGAGACCAACATCCTTAAGCGGCTCTATAAG GCCCTGTCTCTCTTGAAGAAGGAGTTTGAGTTGAGCAAGCTTCAGCAACGCCTAGGCCGTGAGGTAGAGG AGAAGATCAAGCAGACACACAGGAAGTACCTGCTGCAGGAGCAGTTGAAGATCATCAAGAAGGAATTGGG GCTGGAGAAAGATGATAAAGATGCCATTGAGGAGAAGTTCCGGGAACGCCTCAGGGAGCTTGTGGTTCCC AAGCATGTGATGGACGTGGTGGATGAGGAGCTGAGCAAGCTTGCTCTACTGGACAACCACTCCTCTGAGT TCAATGTCACTCGAAACTACCTGGACTGGCTGACATCCATCCCATGGGGCCGGCAGAGTGATGAGAACTT GGACTTGGCTCGGGCCCAGGCTGTGCTGGAGGAGGACCACTACGGCATGGAGGATGTGAAGAAGCGTGTC CTGGAGTTCATTGCAGTCAGCCAGCTCAGAGGCTCCACACAAGGCAAGATCCTCTGCTTCCACGGCCCAC CGGGGGTGGGCAAGACCAGCATTGCACGCTCCATTGCCCGTGCCCTTGGCCGAGAATACTTCCGTTTCAG TGTTGGTGGCATGACAGATGTGGCAGAAATCAAGGGGCACAGGCGTACCTACGTGGGGGCCATGCCTGGG AAGATCATCCAGTGTCTGAAGAAGACCAAGACAGAGAACCCGCTAGTGCTCATTGATGAGGTGGACAAGA TTGGCCGAGGCTACCAAGGGGACCCGTCATCAGCACTGCTGGAGTTGCTGGACCCTGAGCAGAATGCCAA CTTCCTGGACCACTACCTGGATGTGCCAGTGGATCTGTCCAAGGTGCTATTCATCTGCACGGCCAATGTC ATTGACACCATCCCAGAACCACTGAGGGACCGCATGGAGATGATCAATGTGTCTGGCTATGTAGCACAGG AGAAGCTGGCCATCGCAGAGCGGTACCTGGTGCCACAGGCCCGCACCCTGTGTGGCCTAGATGAGAGCAA GGCCCAGCTGTCAGCAGCAGTGCTCACACTGCTCATCAAGCAATACTGCAGGGAGAGTGGTGTGCGCAAC CTACAGAAGCAGGTGGAGAAGGTGCTGCGCAAGGCTGCATACAAGATCGTAAGTGGGGAGGCCCAGACAG TACAGGTGACACCTGAGAACCTACAAGACTTTGTTGGGAAGCCTGTGTTCACAGTGGAGCGCATGTACGA AGTCACACCTCCTGGCGTGGTCATGGGTTTGGCCTGGACTGCCATGGGGGGCTCTACATTGTTTGTGGAG ACATCTTTGAGGCGGCCCCAGCCTAGTGGCAGCAAGGAGGACAAGGATGGCAGCCTAGAGGTGACAGGTC AGCTGGGAGATGTGATGAAAGAGAGTGCCCGCATAGCCTACACCTATGCTCGTGCCTTCCTGATGGAGCA GGACCCTGAAAATGACTTCCTAGTCACCTCACACATCCACCTGCATGTGCCTGAGGGTGCTACCCCCAAG GATGGTCCCAGCGCAGGCTGTACTATTGTCACTGCACTGCTGTCCCTGGCTCTGGGCCAGCCAGTGTTGC AGAACCTGGCTATGACTGGGGAGGTCTCCCTCACTGGCAAGGTGTTGCCTGTGGGTGGCATCAAGGAGAA GACCATTGCGGCTAAGCGTGCAGGTGTGACCTGCATCATTCTGCCTGCTGAGAACAGAAAGGACTACTCG GATTTAGCTCCTTTTATCACAGAGGGCCTTGAAGTTCACTTCGTGGAACACTATCGGGACATATTCCCCA TTGCCTTCCCCCGGCGAGAGCACAGGGAGGCACTGGCCGTGGAGAGA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG215882 representing NM_028782
Red=Cloning site Green=Tags(s) MAASTGYVRLWAAARCWVLRRPLLAVTGGRVPSASGSWLRRGCRACDMSAPWGGRVLPGGVQWRGLWDSG NRGGSDETSEGGAEDGATASTGEGPVVTALAPMTVPDVFPHLPLIAITRNPVFPRFIKIVEVKNKKLVEL LRRKVRLAQPYVGVFLKRDDNNESDVVESLDEIYHTGTFAQIHEMQDLGDKLRMIVTGHRRIHISRQLEV EPEGLEPEAEKQKSRRKLKRGKKEVEDELGPKPQLEMVTEAATDTSKEVLMVEVENVAHEDFQVTEEVKA LTAEIVKTIRDIIALNPLYRESVLQMMQAGQRVVDNPIYLSDMGAALTGAESHELQDVLEETNILKRLYK ALSLLKKEFELSKLQQRLGREVEEKIKQTHRKYLLQEQLKIIKKELGLEKDDKDAIEEKFRERLRELVVP KHVMDVVDEELSKLALLDNHSSEFNVTRNYLDWLTSIPWGRQSDENLDLARAQAVLEEDHYGMEDVKKRV LEFIAVSQLRGSTQGKILCFHGPPGVGKTSIARSIARALGREYFRFSVGGMTDVAEIKGHRRTYVGAMPG KIIQCLKKTKTENPLVLIDEVDKIGRGYQGDPSSALLELLDPEQNANFLDHYLDVPVDLSKVLFICTANV IDTIPEPLRDRMEMINVSGYVAQEKLAIAERYLVPQARTLCGLDESKAQLSAAVLTLLIKQYCRESGVRN LQKQVEKVLRKAAYKIVSGEAQTVQVTPENLQDFVGKPVFTVERMYEVTPPGVVMGLAWTAMGGSTLFVE TSLRRPQPSGSKEDKDGSLEVTGQLGDVMKESARIAYTYARAFLMEQDPENDFLVTSHIHLHVPEGATPK DGPSAGCTIVTALLSLALGQPVLQNLAMTGEVSLTGKVLPVGGIKEKTIAAKRAGVTCIILPAENRKDYS DLAPFITEGLEVHFVEHYRDIFPIAFPRREHREALAVER TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_028782 |
| ORF Size | 2847 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_028782.2, NP_083058.2 |
| RefSeq Size | 2970 bp |
| RefSeq ORF | 2850 bp |
| Locus ID | 74142 |
| UniProt ID | Q8CGK3 |
| Gene Summary | ATP-dependent serine protease that mediates the selective degradation of misfolded, unassembled or oxidatively damaged polypeptides as well as certain short-lived regulatory proteins in the mitochondrial matrix. May also have a chaperone function in the assembly of inner membrane protein complexes. Participates in the regulation of mitochondrial gene expression and in the maintenance of the integrity of the mitochondrial genome. Binds to mitochondrial promoters and RNA in a single-stranded, site-specific, and strand-specific manner. May regulate mitochondrial DNA replication and/or gene expression using site-specific, single-stranded DNA binding to target the degradation of regulatory proteins binding to adjacent sites in mitochondrial promoters.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC222791 | Lonp1 (untagged) - Mouse lon peptidase 1, mitochondrial (Lonp1), nuclear gene encoding mitochondrial protein, (10ug) |
CNY 12,160.00 |
|
| MR215882 | Lonp1 (Myc-DDK-tagged) - Mouse lon peptidase 1, mitochondrial (Lonp1), nuclear gene encoding mitochondrial protein |
CNY 6,952.00 |
|
| MR215882L3 | Lenti ORF clone of Lonp1 (Myc-DDK-tagged) - Mouse lon peptidase 1, mitochondrial (Lonp1), nuclear gene encoding mitochondrial protein |
CNY 9,030.00 |
|
| MR215882L4 | Lenti ORF clone of Lonp1 (mGFP-tagged) - Mouse lon peptidase 1, mitochondrial (Lonp1), nuclear gene encoding mitochondrial protein |
CNY 9,030.00 |
