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Tnpo1 (NM_178716) Mouse Tagged ORF Clone
CAT＃: MG216038
- TrueORF®
Tnpo1 (tGFP-tagged) - Mouse transportin 1 (Tnpo1) transcript variant 1, (10ug)
ORF Plasmid: DDK
"NM_178716" in other vectors (3)
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CNY 8,650.00
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Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AU021749; D13Ertd688e; IPO2; Kpnb2; MIP; MIP1; TRN |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG216038 representing NM_178716
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGTGGGACCGGCAAACCAAGATGGAGTATGAGTGGAAACCTGACGAGCAAGGGCTTCAGCAGATCC TGCAGCTGCTCAAGGAGTCCCAGTCCCCAGACACCACCATCCAGAGGACCGTGCAACAAAAACTGGAACA ACTCAATCAATATCCAGATTTTAACAACTACTTGATTTTTGTTCTTACAAAATTAAAATCTGAAGATGAG CCCACACGGTCATTGAGCGGTCTGATCTTGAAGAACAATGTGAAAGCTCATTTTCAGAACTTCCCAAATG GTGTGACAGACTTCATCAAGAGTGAATGTCTAAATAACATTGGGGACTCCTCTCCGTTGATCAGAGCCAC TGTCGGTATTTTAATTACAACCATAGCCTCCAAGGGAGAGCTGCAGAATTGGCCTGATCTCTTACCAAAA CTCTGTAGCCTGCTGGATTCCGAAGACTACAACACTTGTGAGGGAGCCTTCGGTGCCCTTCAGAAGATAT GTGAGGACTCTGCAGAGATTTTAGACAGTGATGTCTTAGATCGCCCTCTCAACATCATGATCCCCAAGTT TTTACAGTTTTTCAAGCACAGCAGTCCCAAAATAAGGTCTCATGCTGTTGCATGTGTCAATCAGTTCATC ATCAGTCGGACCCAGGCGCTCATGCTGCACATCGATTCCTTCATTGAGAACCTCTTTGCACTGGCTGGCG ACGAGGAAGCAGAGGTGCGGAAGAACGTGTGCCGGGCGCTTGTGATGTTGCTTGAAGTCCGGATGGATCG CCTGCTTCCTCATATGCACAACATAGTCGAGTACATGCTGCAGAGAACCCAAGACCAAGATGAGAATGTA GCTCTGGAGGCCTGTGAATTCTGGCTGACTTTGGCTGAACAGCCAATATGCAAAGATGTACTTGTAAGGC ATCTACCAAAGTTGATTCCTGTGTTAGTGAATGGCATGAAGTACTCAGATATAGATATTATCCTGCTTAA GGGTGATGTTGAGGAAGATGAGACCATCCCGGATAGTGAGCAGGATATACGGCCGCGGTTTCATCGCTCG AGGACAGTGGCTCAGCAGCATGAGGAGGATGGGATTGAGGAGGAAGACGATGATGATGACGAAATTGATG ATGATGACACGATTTCTGACTGGAACCTGAGAAAGTGTTCTGCTGCTGCTCTCGATGTTCTCGCAAATGT TTATCGTGATGAGCTTTTGCCGCACATTCTGCCACTTTTGAAAGAATTGCTTTTCCATCATGAATGGGTT GTGAAAGAATCTGGCATCTTGGTTTTAGGAGCAATTGCTGAAGGTTGTATGCAAGGCATGATTCCGTACC TGCCCGAGCTCATTCCTCACCTTATTCAGTGCCTTTCTGATAAAAAGGCTCTTGTGCGTTCCATCACCTG CTGGACTCTTAGCCGCTATGCACACTGGGTAGTCAGCCAGCCACCAGATACGTACCTGAAGCCATTAATG ACAGAACTGCTGAAGCGTATCCTCGATAGCAACAAGAGAGTACAGGAAGCAGCTTGCAGTGCCTTCGCTA CATTAGAAGAGGAGGCTTGTACAGAGCTCGTCCCTTACCTTGCTTATATACTCGATACCCTCGTCTTCGC CTTCAGTAAATACCAGCATAAGAACCTGCTCATTCTGTACGACGCCATAGGGACACTGGCAGATTCAGTG GGACATCATTTAAACAAGCCAGAATATATTCAGATGCTAATGCCTCCTTTGATCCAGAAATGGAACATGC TGAAGGATGAAGACAAGGATCTTTTCCCTTTGCTTGAGTGTCTCTCGTCTGTTGCCACAGCCTTGCAGTC TGGCTTCCTTCCATATTGTGAACCTGTATATCAGCGTTGCGTAAACCTAGTTCAGAAGACTCTGGCACAA GCCATGCTAAACAATGCTCAACCAGAACAGTATGAAGCTCCAGATAAAGATTTTATGATTGTGGCTCTTG ACTTACTCAGCGGCCTGGCTGAGGGCCTGGGAGGCAACATTGAGCAGCTGGTGGCCCGCAGTAACATCCT GACGCTGATGTACCAGTGCATGCAGGATAAAATGCCCGAGGTTCGGCAGAGTTCTTTTGCATTGCTAGGT GACCTGACTAAAGCGTGCTTCCAGCATGTTAAGCCTTGTATAGCTGATTTCATGCCAATATTGGGAACCA ATCTAAATCCAGAGTTTATTTCAGTCTGCAACAATGCCACCTGGGCGATTGGGGAAATATCAATCCAAAT GGGTATAGAGATGCAGCCCTACATCCCTATGGTGTTGCACCAGCTTGTGGAGATCATTAACAGACCCAAC ACCCCAAAGACGCTGTTGGAGAACACAGCAATAACAATTGGTCGTCTTGGTTACGTTTGTCCTCAAGAGG TGGCCCCCATGCTACAGCAGTTTATAAGACCCTGGTGTACCTCTCTGAGAAACATAAGAGACAATGAAGA AAAAGATTCAGCATTCCGTGGGATTTGTACCATGATCAGTGTGAATCCCAGTGGCGTAATCCAAGATTTT ATATTTTTTTGTGATGCTGTTGCATCATGGATTAACCCAAAAGATGATCTCAGAGACATGTTCTGTAAGA TCCTTCATGGATTTAAAAACCAAGTTGGGGATGAAAATTGGAGGCGATTCTCTGACCAGTTTCCTCTTCC CTTAAAAGAGCGTCTTGCAGCTTTTTATGGTGTT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG216038 representing NM_178716
Red=Cloning site Green=Tags(s) MVWDRQTKMEYEWKPDEQGLQQILQLLKESQSPDTTIQRTVQQKLEQLNQYPDFNNYLIFVLTKLKSEDE PTRSLSGLILKNNVKAHFQNFPNGVTDFIKSECLNNIGDSSPLIRATVGILITTIASKGELQNWPDLLPK LCSLLDSEDYNTCEGAFGALQKICEDSAEILDSDVLDRPLNIMIPKFLQFFKHSSPKIRSHAVACVNQFI ISRTQALMLHIDSFIENLFALAGDEEAEVRKNVCRALVMLLEVRMDRLLPHMHNIVEYMLQRTQDQDENV ALEACEFWLTLAEQPICKDVLVRHLPKLIPVLVNGMKYSDIDIILLKGDVEEDETIPDSEQDIRPRFHRS RTVAQQHEEDGIEEEDDDDDEIDDDDTISDWNLRKCSAAALDVLANVYRDELLPHILPLLKELLFHHEWV VKESGILVLGAIAEGCMQGMIPYLPELIPHLIQCLSDKKALVRSITCWTLSRYAHWVVSQPPDTYLKPLM TELLKRILDSNKRVQEAACSAFATLEEEACTELVPYLAYILDTLVFAFSKYQHKNLLILYDAIGTLADSV GHHLNKPEYIQMLMPPLIQKWNMLKDEDKDLFPLLECLSSVATALQSGFLPYCEPVYQRCVNLVQKTLAQ AMLNNAQPEQYEAPDKDFMIVALDLLSGLAEGLGGNIEQLVARSNILTLMYQCMQDKMPEVRQSSFALLG DLTKACFQHVKPCIADFMPILGTNLNPEFISVCNNATWAIGEISIQMGIEMQPYIPMVLHQLVEIINRPN TPKTLLENTAITIGRLGYVCPQEVAPMLQQFIRPWCTSLRNIRDNEEKDSAFRGICTMISVNPSGVIQDF IFFCDAVASWINPKDDLRDMFCKILHGFKNQVGDENWRRFSDQFPLPLKERLAAFYGV TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_178716 |
| ORF Size | 2694 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_178716.3, NP_848831.2 |
| RefSeq Size | 5391 bp |
| RefSeq ORF | 2697 bp |
| Locus ID | 238799 |
| UniProt ID | Q8BFY9 |
| Gene Summary | Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates (PubMed:11493596). Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (By similarity). Involved in nuclear import of M9-containing proteins. In vitro, binds directly to the M9 region of the heterogeneous nuclear ribonucleoproteins (hnRNP), A1 and A2 and mediates their nuclear import. Appears also to be involved in hnRNP A1/A2 nuclear export. Mediates the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5. Binds to a beta-like import receptor binding (BIB) domain of RPL23A (By similarity). In vitro, mediates nuclear import of SRP19 (By similarity). Mediates the import of histones H2A, H2B, H3 and H4 (PubMed:11493596). Mediates nuclear import of ADAR/ADAR1 in a RanGTP-dependent manner (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MR216038 | Tnpo1 (Myc-DDK-tagged) - Mouse transportin 1 (Tnpo1), transcript variant 1 |
CNY 6,576.00 |
|
| MR216038L3 | Lenti ORF clone of Tnpo1 (Myc-DDK-tagged) - Mouse transportin 1 (Tnpo1), transcript variant 1 |
CNY 9,790.00 |
|
| MR216038L4 | Lenti ORF clone of Tnpo1 (mGFP-tagged) - Mouse transportin 1 (Tnpo1), transcript variant 1 |
CNY 9,790.00 |
