Arap1 (NM_198096) Mouse Tagged ORF Clone
CAT#: MG216526
- TrueORF®
Arap1 (tGFP-tagged) - Mouse ArfGAP with RhoGAP domain ankyrin repeat and PH domain 1 (Arap1) transcript variant 2, (10ug)
ORF Plasmid: DDK
"NM_198096" in other vectors (4)
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CNY 15,770.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | 2410002L19Rik; Centd2; mKIAA0782 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG216526 representing NM_198096
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGAGGGTTACGATGCCGCACTGTCTGTAGCTGAGTGGCTGCGGGCACTTCACCTGGAGCAGTACA CGGCACTCTTTGAGCAGCACGGACTGGTGTGGGCCACCGAGTGCCAAGGCCTCAGCGATGCTGGCCTGCT GGACATGGGCATGCACCTCCCCGGCCACCGCCGCCGCATCCTGGCTGGTCTTCACCGCGCCCATGCACCC CCGGTCCCTCTGCCTCGCCCCGCCCCACGGCCCGTGCCGATGAAGCGACACATCTTCCGTTCACCACCTG TGCCCGTCACTCCACCAGAGCCGCCGCCCACAGCTGGAGAGGATGAGGGTCTGCCTGCCGCTCCACCTAT CCCACCCCGCAGGAGCTGCCTCCCACCTGCCTGCTTCACTCCGACATCCACAGCCGCCCCAGATCCTGTG CTGCCCCCACTGCCTGCCAAGAGACACTTGGTAGAATCCAGCGTTCCTCCTGTCCCTCCTCGAACCGGAC CCCCCTACCCACAGGCCAGCCTTCTTGCTAAGGAGGAGTTACTGCTGCCATCAGTGTCACCCCGGTCCCA ACCAGAGCCTGCAGAGACTCCGTCCACCCTCCTGCCTGCCTTTCCCCAGGGGCCCCTGCAGCCTCCGTCT CCTCCTCCCTGTCCTCCTGTGATCCCTCCCAAGCCTCCTCGCCTGCTCCCTGAGTTTGACGACTCTGACT ATGATGATGTCCCGGAGGAGGGGCCAGGGGCCCCAGCCAGTGTGATGACCAAGGAGGAGCCCCTGCCGAG CCGAGTCCCACGGGCTGTGCGTGTGGCTAGTCTGCTGAGCGAGGGGGAGGAGCTGTCTGGGGATGATGCG GAAGATGACGATGACCATGCCTATGAGGGTATCCCCAATGGTGGGTGGCCAACGAGTGGCCTGAATCCAC CCTTACGCAGCCTGATCCCTGATCTCCCACTGCACCCCATGGATGAGTTGCCTGGGGGTCCCACCCCCAT CACACCTGTCATCAAGGCTGGCTGGCTGGACAAGAACCCACCACAGGGGTCTTATATCTATCAGAAGCGA TGGGTGAGACTGGACGCTGATTACCTGCGATACTTTGACAGTAACAAGGACGCCTACTCTAAGCGCTTTG TTCCTGTGGCCTGCATCTGCCGAGTAGCTCCTATTGGAGACCAGAAGTTTGAAGTGATCACAAATAATCG GACCTTCGCCTTCCGGGCAGAGAGTGATGTGGAGCGGAACGAGTGGATGCAGGCCCTGCAGCAGGCAGTA GTTGAGCATCGAGCCCGTTTTCGGCTTTCTAGTGCTTCTGTGTTGGGAGTCCGAGGCTCCGAGCAGCCTG ACCGTGCTGGCAGCCTGGAGCTACGAGGCTTCAAGAATAAGCTTTACGTGGCTGTGACTGGGGACAAAGT ACAGCTCTATAAGAATCTGGAGGAATTCCATCTGGGCATTGGTATCACCTTTATCGACATGAATGTGGGC AATGTCAAGGAAGTGGATCGGCGCAGCTTTGACCTCACTACCCCCTACCGCATCTTCAGCTTCTCGGCTG ATTCAGAGTTAGAGAAGGAACAGTGGCTGGAGGCCATGCAGGGAGCCATCGCAGAGGCCCTATCTACCTC AGAGGTGGCTGAGCGCATCTGGGCCGCAGCCCCCAACAGGTTCTGTGCCGACTGTGGGGCCGCCCAGCCT GACTGGGCCTCCATCAACCTCTGCGTTGTCATCTGCAAGCGCTGTGCAGGGGAGCACCGCGGCCTGGGTG CAGGAGTGTCCAAGGTGCGGAGTTTGAAGATGGACAGGAAGGTGTGGACAGAAGCACTCATCCAGCTCTT CTTACATCTGGGCAATGGCCCCGGGAACCACTTCTGGGCTGCTAATGTGCCTCCTAGTGAGGCCCTGGAG CCCAGCAGCAGCCCTGGTGCCCGGCGGTATCACCTGGAGGCCAAGTACAGAGAGGGCAAGTACCGCCGCT ACCATCCGCTCTTTGGCAACCAAGAGGAACTGGACAAGGCCCTGTGTGCTGCAGTTACTACCACTGACCT GGCTGAGACCCAGGCACTCCTGGGCTGTGGGGCTGGGGTCAGCTGCTTCTCAGGGGACCCAGCAGCTCCC ACACCCCTGGCTCTTGCTGAGCAGGCCGGACAGACTCTGCAGATGGAGTTTCTACGGAATAATCAGAGTA CAGAGGTCCCTCGGTTGGACTCAGTGAAGCCCTTGGAAAAGCACTACTCCGTTACCCTGCCAACTGTGAG CCACAGCGGCTTCCTGTACAAAACTGCTTCTGCCGGCAAACCTCTGCAGGACCGCCGTGCCCGGGAAGAG TTCAGCCGGCGCTGGTGTGTCCTGAGTGATGGGGTCCTGAGCTACTATGAAAACGAACGGGCAGTGACAC CCAATGGGGAGATTCGGGCCAGCGAGATAGTATGCCTAGCAGTTTCCCCTCTGGACACCCATGGCTTTGA GCACACCTTTGAGGTGTACACAGAGGGAGAACGGCTGTACCTGTTTGGGCTGGAGAATGCAGAGCTGGCT CATGAGTGGGTCAAGTGCATTGCCAAGGCGTTCGTGCCTCCCCTGGCTGAGGACCTGCTAGCCCGGGACT TTGAACGTCTTGGGCGCCTACCCTGTAAAGCTGGCCTGAGCCTTCAGCAGGCTCAGGAAGGCTGGTTTGC CTTGACTGGCTCTGAGCTCCGGGCTGTCTTCCCAGAGGGGCCCTGGGAAGAGCCGCTGCAGCTCCGGAAA CTGCAAGAGCTTTCTATCCAAGGAGACAGCGAGAACCAAGTGCTGGTGCTGGTGGAGCGGAGGAGGACAC TGTACATCCAGGGTGAGCGGCGGCTGGACTTCATGGCTTGGCTAGGGGTCATCCAGAAAGCAGCAGCCAG CTTGGGAGACACACTATCAGAACAACAGCTTGGGGACTCGGACATCCCAGTGATTGTGTACCGCTGTGTG GACTACATCACGCAGTGTGGCTTGACCTCAGAGGGTATCTATCGAAAGTGTGGTCAGACCTCCAAGACTC AGAGACTGCTAGACAGCCTCCGGCAGGACGCACGCTCTGTGCACCTAAAGGAGGGAGAACAGCACGTGGA CGACGTCTCCTCTGCACTCAAACGCTTCCTCAGGGACCTGCCCGATGGGCTCTTCACGCGTGCGCAGCGC CTGGCCTGGCTGGAGGCCTCTGAGATCGAGGATGAGGAAGAAAAGATCTCCAGGTATCGAGAGCTCTTGG TGCATCTGCCCCCTGTCAACCGGGCCACTGTGAAGGCCCTTATCAGCCATCTGTACTGTGTACAGTGCTT CTCAGACACAAACCAAATGAACACACACAACCTGGCTATCGTGTTTGGGCCTACACTCTTCCAGACAGAC GGGCAGGACTACAAGGCCGGCAAGGTGGTGGAAGACCTCATCAACCACTACGTGGTGGTGTTCAGTGTGG ACGAGGAGGAGCTGAGGAAGCAGAGGGAGGAAGTCACGGCCATCGTGAAGATGCGAGTGGCTGGCACTGC CAGTGGGACCCAGCATGCTGGCGACTTCATCTGCACAGTCTACCTGGAGGAGAAGAAGGTGGAGACTGAA CAGCATGTTAAGATCCCAGCATCCATGACTGCAGAGGAGCTTACTCTGGAGATTCTGGACCGCCGCAATG TGAGCATCAGGGAGAAGGACTACTGGACTTGCTTTGAGGTCAACGAGAAGGAGGAGGCAGAGCGCCCGCT GCACTTTGCAGAGAAGGTGCTGCCCATTGTCCATGGGCTGGGCATAGACAGCCATCTGGTGGTGAAGAAG TACCAGTCCATGGAGGCCATGCTGTTGTACTTGGCCAGCCGTGTGGGTGACACCAAGCATGGTATGATGA AGTTCCGTGAAGACCGCAGCCTCCTGGGTCTGGGGCTACCTTCGGGTGGCTTCCACGATCGCTACTTCAT TCTCAACAGCAGCTGCCTACGGCTCTACAAGGAGGTCCGGAGTCACCGGCCTGAGAAGGAGTGGCCTGTC AAGAGCCTCAAAGTCTACCTGGGTGTAAAGAAGAAACTGCGGCCACCTACTTGCTGGGGCTTCACGGTGG TGCACGAGACAGAAAAGCACGAGAAGCAGCAGTGGTACCTCTGCTGTGACACGCAGATGGAACTTCGAGA GTGGTTTGCCACCTTCCTCTCTGTGCAGCACGATGGCCTGGTGTGGCCCTCCGAGCCATCTCGAGTGTCC CGGGCAGTGCCTGAGGTCCGGATGGGCAGTGTATCGCTGATCCCTCTACGAGGCAGTGAAAATGAAATGC GCCGGAGTGTGGCAGCCTTCACTGCTGACCCCCTTTCCCTTCTCCGCCATGTC AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG216526 representing NM_198096
Red=Cloning site Green=Tags(s) MAEGYDAALSVAEWLRALHLEQYTALFEQHGLVWATECQGLSDAGLLDMGMHLPGHRRRILAGLHRAHAP PVPLPRPAPRPVPMKRHIFRSPPVPVTPPEPPPTAGEDEGLPAAPPIPPRRSCLPPACFTPTSTAAPDPV LPPLPAKRHLVESSVPPVPPRTGPPYPQASLLAKEELLLPSVSPRSQPEPAETPSTLLPAFPQGPLQPPS PPPCPPVIPPKPPRLLPEFDDSDYDDVPEEGPGAPASVMTKEEPLPSRVPRAVRVASLLSEGEELSGDDA EDDDDHAYEGIPNGGWPTSGLNPPLRSLIPDLPLHPMDELPGGPTPITPVIKAGWLDKNPPQGSYIYQKR WVRLDADYLRYFDSNKDAYSKRFVPVACICRVAPIGDQKFEVITNNRTFAFRAESDVERNEWMQALQQAV VEHRARFRLSSASVLGVRGSEQPDRAGSLELRGFKNKLYVAVTGDKVQLYKNLEEFHLGIGITFIDMNVG NVKEVDRRSFDLTTPYRIFSFSADSELEKEQWLEAMQGAIAEALSTSEVAERIWAAAPNRFCADCGAAQP DWASINLCVVICKRCAGEHRGLGAGVSKVRSLKMDRKVWTEALIQLFLHLGNGPGNHFWAANVPPSEALE PSSSPGARRYHLEAKYREGKYRRYHPLFGNQEELDKALCAAVTTTDLAETQALLGCGAGVSCFSGDPAAP TPLALAEQAGQTLQMEFLRNNQSTEVPRLDSVKPLEKHYSVTLPTVSHSGFLYKTASAGKPLQDRRAREE FSRRWCVLSDGVLSYYENERAVTPNGEIRASEIVCLAVSPLDTHGFEHTFEVYTEGERLYLFGLENAELA HEWVKCIAKAFVPPLAEDLLARDFERLGRLPCKAGLSLQQAQEGWFALTGSELRAVFPEGPWEEPLQLRK LQELSIQGDSENQVLVLVERRRTLYIQGERRLDFMAWLGVIQKAAASLGDTLSEQQLGDSDIPVIVYRCV DYITQCGLTSEGIYRKCGQTSKTQRLLDSLRQDARSVHLKEGEQHVDDVSSALKRFLRDLPDGLFTRAQR LAWLEASEIEDEEEKISRYRELLVHLPPVNRATVKALISHLYCVQCFSDTNQMNTHNLAIVFGPTLFQTD GQDYKAGKVVEDLINHYVVVFSVDEEELRKQREEVTAIVKMRVAGTASGTQHAGDFICTVYLEEKKVETE QHVKIPASMTAEELTLEILDRRNVSIREKDYWTCFEVNEKEEAERPLHFAEKVLPIVHGLGIDSHLVVKK YQSMEAMLLYLASRVGDTKHGMMKFREDRSLLGLGLPSGGFHDRYFILNSSCLRLYKEVRSHRPEKEWPV KSLKVYLGVKKKLRPPTCWGFTVVHETEKHEKQQWYLCCDTQMELREWFATFLSVQHDGLVWPSEPSRVS RAVPEVRMGSVSLIPLRGSENEMRRSVAAFTADPLSLLRHV SGPTRTRRLE - GFP Tag - V |
Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_198096 |
ORF Size | 2181 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_198096.1, NP_932764.1 |
RefSeq Size | 3673 bp |
RefSeq ORF | 2184 bp |
Locus ID | 69710 |
UniProt ID | Q4LDD4 |
Gene Summary | Phosphatidylinositol 3,4,5-trisphosphate-dependent GTPase-activating protein that modulates actin cytoskeleton remodeling by regulating ARF and RHO family members. Is activated by phosphatidylinositol 3,4,5-trisphosphate (PtdIns(3,4,5)P3) binding. Can be activated by phosphatidylinositol 3,4-bisphosphate (PtdIns(3,4,5)P2) binding, albeit with lower efficiency. Has a preference for ARF1 and ARF5 (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC221103 | Arap1 (untagged) - Mouse ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 1 (Arap1), transcript variant 2, (10ug) |
CNY 9,310.00 |
|
MR216526 | Arap1 (Myc-DDK-tagged) - Mouse ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 1 (Arap1), transcript variant 2 |
CNY 14,350.00 |
|
MR216526L3 | Lenti ORF clone of Arap1 (Myc-DDK-tagged) - Mouse ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 1 (Arap1), transcript variant 2 |
CNY 16,250.00 |
|
MR216526L4 | Lenti ORF clone of Arap1 (mGFP-tagged) - Mouse ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 1 (Arap1), transcript variant 2 |
CNY 16,250.00 |