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Orc2 (NM_001025378) Mouse Tagged ORF Clone
CAT＃: MG217216
- TrueORF®
Orc2 (tGFP-tagged) - Mouse origin recognition complex subunit 2-like (S. cerevisiae) (Orc2l) transcript variant b, (10ug)
ORF Plasmid: DDK
"NM_001025378" in other vectors (3)
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CNY 5,800.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AU041563; Orc2l |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG217216 representing NM_001025378
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCACTCTGCAGTTAAAGGAAACCAAGGTGCCAAGCGTGCAGTTTGTGGGAGATGATGATGTTCTTA GTCACATTCTGGACAGAGAAGGAGAATCCTTGGGAAACGGCTCTGCTAAAGATGGTAGGAATAAAGTTTA TTCTTTTCAACAAAGAAAGCACCCTGAAGAGATGACTAAATTGGCTTTAGAACTAGCAAAAACATCAGGA AAAAAGGATCCACTTGACTCAAATGATCCTGAAATCACAAAAAACATTGCTCAAAAAAGTAAAGGGCATT CTACTTCAGAGAAAGCTCCACTGGTGAACAATAACAAAACTGAATTTCTGTCAACACAACCTCATAATCT AAGGAAAAGAATAATAGCGTCAAGGTCTCATTATGACAGTGAGAGTGAGTATTCTGCTTCCAGCTCAGAG GATGATGAAGAGGCTACAAAAGACGAAGAAGAAGACACTAATGTGGCCAGGCTCTCTCAGAAGAGTCAAG GTCAGAATAGACTTCTTCCAGCTCCTGTTTCCAAAGAGACACTGCCTAAGAAAAAGAAAAGAGACAAAGC GAGTGACTTGGTAGAAGAATATTTTGAAGCCCATAGTAGTTCAAAAGTCTTAACATCTGATCGAACGCTG CAGAGGCTGAGGAGAGCCAGAGTGGATCAGAAAACTCTGCATAACTTACTGAGAAAATTTGTGCCTTCCT TTTCTGCTGAAATTGAACGATTGAATCAACAGCATGAGAAACTGTTTCATAAATGGATGCTTCAGTTACA CCTTGGGTTCAACATTGTGCTTTATGGCTTGGGGTCCAAGAGAGATTTACTAGAAAAGTTTCGAACCACC ATGCTTCAAGATTCCATTCATGTTGTCATCAACGGCTACTTTCCTGGCGTCAGTGTGAAATCAATCCTGA ACTCTATAACTGAAGATGTCCTCAGTCATGTGGGTACTTTCCAAAGTGTTCTGGATCAGCGGGACTGGAT AATAAACAGATTTAAAGAAGATTCTTCTTTAGAACTCTTCCTTCTTATCCACAATTTGGATAGCCAAATG TTAAGAGGAGACAATAGCCAGCAAATTCTTGGACAGTTGTCATCATTACATAACGTGTATCTCATAGCAT CTATTGACCATCTCAATGCTCCTCTCATGTGGGATCATGCAAAGCAGAGTCTTTATAACTGGCTTTGGTA TGAAACTACTACATACAGTCCTTATACTGAAGAAACCTCCTATGAGAACTCCCTTCTGGTTAAGCAGTCT GGATCTCTGCCACTTAGTTCTCTGATTCATGTCTTACGAAGCCTTACCCCCAATGCAAGGGGGATTTTCA GGCTACTTATGAAGTTCCAGCTGGATAACCAGGACAGCCCTTCCTACATTGGACTTTCTTTTCAAGATTT TTACCAGCAGTGTAGGGAAGCATTCCTTGTTAACAGTGATCTCACACTCCGAGCCCAGTTAACTGAATTT AGGGACCACAAACTTATAAGAACAAAGAAGGGAACTGATGGTGTAGAATATTTATTAATTCCTGTTGACA GCGGAATATTGGCTGATTTCTTGGAGAAGGAAGAGGAGGAGGCA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG217216 representing NM_001025378
Red=Cloning site Green=Tags(s) MSTLQLKETKVPSVQFVGDDDVLSHILDREGESLGNGSAKDGRNKVYSFQQRKHPEEMTKLALELAKTSG KKDPLDSNDPEITKNIAQKSKGHSTSEKAPLVNNNKTEFLSTQPHNLRKRIIASRSHYDSESEYSASSSE DDEEATKDEEEDTNVARLSQKSQGQNRLLPAPVSKETLPKKKKRDKASDLVEEYFEAHSSSKVLTSDRTL QRLRRARVDQKTLHNLLRKFVPSFSAEIERLNQQHEKLFHKWMLQLHLGFNIVLYGLGSKRDLLEKFRTT MLQDSIHVVINGYFPGVSVKSILNSITEDVLSHVGTFQSVLDQRDWIINRFKEDSSLELFLLIHNLDSQM LRGDNSQQILGQLSSLHNVYLIASIDHLNAPLMWDHAKQSLYNWLWYETTTYSPYTEETSYENSLLVKQS GSLPLSSLIHVLRSLTPNARGIFRLLMKFQLDNQDSPSYIGLSFQDFYQQCREAFLVNSDLTLRAQLTEF RDHKLIRTKKGTDGVEYLLIPVDSGILADFLEKEEEEA TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001025378 |
| ORF Size | 1584 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001025378.2, NP_001020549.1 |
| RefSeq Size | 3030 bp |
| RefSeq ORF | 1587 bp |
| Locus ID | 18393 |
| Gene Summary | Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent. The specific DNA sequences that define origins of replication have not been identified yet. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication (By similarity). Binds histone H3 and H4 trimethylation marks H3K9me3, H3K20me3 and H4K27me3. Stabilizes LRWD1, by protecting it from ubiquitin-mediated proteasomal degradation. Also stabilizes ORC3 (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MR217216 | Orc2 (Myc-DDK-tagged) - Mouse origin recognition complex, subunit 2 (Orc2), transcript variant b |
CNY 5,230.00 |
|
| MR217216L3 | Lenti ORF clone of Orc2 (Myc-DDK-tagged) - Mouse origin recognition complex, subunit 2 (Orc2), transcript variant b |
CNY 7,130.00 |
|
| MR217216L4 | Lenti ORF clone of Orc2 (mGFP-tagged) - Mouse origin recognition complex, subunit 2 (Orc2), transcript variant b |
CNY 7,130.00 |
