Kdm2b (NM_001005866) Mouse Tagged ORF Clone
CAT#: MG217278
- TrueORF®
Kdm2b (tGFP-tagged) - Mouse lysine (K)-specific demethylase 2B (Kdm2b) transcript variant 3, (10ug)
ORF Plasmid: DDK
"NM_001005866" in other vectors (3)
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CNY 14,350.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | Cxxc2; E430001G17; Fbl10; Fbxl10; Jhdm1b; mKIAA3014; PCCX2 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG217278 representing NM_001005866
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGCAGCTGCTGCGTGCGATTGACCGCCAGAGATACGACGAGAACGAAGACTTGTCGGACGTGGAGG AAATTGTCAGCGTCCGTGGCTTCAGCCTGGAGGAGAAGCTACGTAGCCAGTTATACCAGGGGGACTTCGT GCATGCTATGGAAGGCAAAGATTTTAACTATGAGTACGTACAGAGAGAAGCTCTCAGGGTCCCCCTGGTT TTTCGGGACAAGGATGGACTAGGGATCAAGATGCCAGACCCTGATTTCACAGTCCGAGACGTCAAACTCC TGGTGGGGAGCCGCCGTTTGGTGGATGTCATGGACGTCAACACCCAGAAGGGTACCGAGATGAGCATGTC CCAGTTTGTGCGCTACTACGAGACACCAGAGGCACAGCGGGATAAACTGTACAACGTCATCAGCCTCGAG TTCAGCCATACGAAGCTGGAGCATCTGGTCAAGCGTCCCACTGTGGTGGACCTGGTCGACTGGGTGGACA ACATGTGGCCTCAGCATCTAAAGGAAAAGCAGACAGAAGCCACCAATGCCCTTGCAGAGATGAAGTACCC CAAAGTGAAAAAGTACTGTCTGATGAGCGTGAAGGGCTGTTTCACTGACTTCCACATTGACTTTGGAGGC ACCTCCGTGTGGTACCATGTGTTCCGTGGTGGCAAGATCTTTTGGCTGATCCCTCCAACCCTGCACAACT TGGCTTTGTACGAGGAGTGGGTGCTGTCTGGCAAACAGAGCGACATCTTTCTGGGAGACCGCGTGGAACG CTGCCAAAGAATTGAGCTGAAGCAAGGCTACACCTTTTTCATCCCTTCCGGTTGGATCCATGCGGTTTAT ACGCCTGTGGACTCTCTGGTGTTCGGCGGGAACATCCTGCATAGCTTCAACGTGCCCATGCAGCTGCGGA TCTACGAGATCGAGGACAGGACCCGGGTTCAGCCCAAGTTCCGTTACCCCTTCTACTATGAGATGTGCTG GTATGTCTTGGAGAGATATGTGTACTGTGTGACCCAGCGCTCCTACCTCACTCAGGAATACCAGAGAGAA TTAATGCTTATTGATGCCCCAAGAAAAACCAGTGTAGACGGCTTTTCATCCGACTCCTGGCTGGACATGG AGGAGGAGTCCTGCGAGCAGCAGCCACAGGAGGAAGAGGAGGAGGAGGAGGACAAGGAGGAGGAAGGAGA TGGTGCAGATAAAACACCCAAGCCACCCACCGATGACCCCACCTCACCCACCAGCACCCCGCCCGAAGAC CAGGACAGCACAGGGAAGAAGCCTAAAGCCCCTGCCATACGGTTCCTCAAGAGGACGTTGTCCAATGAGT CAGAGGAAAGTGTCAAGTCGACCTCGATGCCCACGGACGATCCCAAGACGCCCACGGGCTCCCCGGCCAC CGAGGTTTCTACCAAGTGGACTCACCTTACCGAATTTGAACTGAAGGGCTTGAAAGCCCTGGTTGAAAAG CTAGAGTCCCTTCCGGAGAATAAGAAGTGTGTCCCTGAGGGAATCGAGGACCCCCAGGCCCTCCTGGAAG GTGTAAAGAATGTACTGAAAGAGCACGTGGATGACGACCCCACCCTGGCCATCACCGGGGTCCCTGTGGT CAGCTGGCCAAAGAAAACTGCAAAGAACCGGGTGGTGGGTCGGCCTAAGGGCAAGTTGGGCCCGGCCTCA GCGGTGAAGTTGGCTGCCAACCGAACAACAGCAGGAGCTCGCAGGCGCCGGACGCGATGCCGCAAGTGCG AGGCCTGCCTGCGGACGGAGTGTGGAGAGTGCCACTTTTGCAAGGACATGAAGAAGTTTGGAGGTCCTGG GCGCATGAAGCAGAGCTGCATCATGCGGCAGTGCATCGCGCCAGTGCTGCCCCACACCGCCGTGTGCCTT GTGTGTGGCGAGGCAGGGAAGGAGGACACAGTGGAAGAGGAAGAAGGCAAGTTTAACCTCATGCTCATGG AATGCTCCATCTGCAACGAGATCATCCACCCTGGATGCCTTAAGATTAAGGAATCGGAGGGTGTGGTCAA CGATGAGCTTCCCAACTGCTGGGAGTGTCCGAAGTGTAACCATGCCGGCAAGACCGGGAAACAAAAGCGT GGCCCTGGCTTTAAGTATGCCTCCAACCTGCCTGGCTCCTTGCTCAAGGAGCAGAAGATGAACCGGGACA ACAAGGAAGGGCAAGAGCCTGCCAAGCGGAGAAGTGAGTGTGAAGAGGCTCCCCGTCGGAGGTCAGACGA GCACCCCAAAAAGGTGCCTGCAGATGGCATCCTCCGCCGAAAGTCTGATGATGTGCACCTGAGGAGGAAG CGGAAATACGAGAAGCCCCAAGAGCTGAGTGGACGCAAGCGAGCCTCGTCGCTTCAAACGTCCCCCGGTT CCTCCTCTCACCTCTCGCCGAGGCCCCCTCTAGGCAGCAGTCTCAGCCCTTGGTGGAGATCCAGTCTCAC TTACTTCCAGCAGCAGCTAAAACCTGGCAAAGAAGATAAGCTTTTCAGGAAAAAGCGGCGGTCCTGGAAG AACGCTGAGGATCGTCTGTCACTGGCCAACAAGCCCCTTCGGCGCTTTAAGCAGGAACCGGAGGACGATC TGCCTGAGGCACCTCCTAAGACCCGGGAGAGTGATCAGTCACGTTCCAGCTCACCCACTGCTGGTCCCAG CACTGAGGGAGCTGAAGGCCCAGAAGAGAAGAAAAAGGTGAAGATGCGCCGGAAGCGGCGACTTGTTAAC AAGGAGCTGAGCAAAGAGCTAAGCAAGGAGCTCAACCATGAGATCCAAAAGACGGAGAGCACCCTGGCTC ACGAGAGCCAGCAGCCCATCAAGTCAGAGCCTGAGAGCGAGAACGACGAGCCCAAGAGGCCCTTAAGCCA CTGCGAGCGCCCCCACCGCTTCAGCAAAGGGCTCAACGGCACACCTCGGGAGCTGCGGCACTCGCTGGGA CCTGGCCTGCGTAGTCCACCTCGTGTTATGTCCCGGCCCCCGCCCTCTGCATCCCCACCCAAGTGCATCC AGATGGAGCGTCACGTGATCCGGCCACCGCCCATCAGCCCCCCACCTGACTCGCTGCCCCTGGATGATGG AGCAGCCCACGTCATGCATAGGGAGGTGTGGATGGCAGTCTTCAGCTACCTCAGCCACCGAGACCTGTGT GTCTGCATGCGGGTCTGCAGGACCTGGAACCGCTGGTGCTGCGATAAGCGGTTGTGGACCCGCATCGACC TGAACCGCTGCAAGTCCATCACACCCCTGATGCTGAGCGGTATCATCCGGCGACAGCCTGTCTCCCTTGA CCTCAGTTGGACCAACATCTCCAAGAAGCAGCTGAGTTGGCTCATCAACCGGTTGCCTGGGCTCCGAGAC TTGGTGCTGTCAGGCTGCTCATGGATCGCTGTCTCAGCCCTCTGTAGCTCCAGTTGTCCATTGCTCCGGA CCCTGGATGTCCAGTGGGTAGAAGGACTAAAGGATGCCCAGATGAGGGATCTCCTGTCTCCACCCACAGA CAACAGGCCAGGTCAGATGGACAATCGGAGCAAGCTCCGGAACATTGTAGAGCTGCGCCTAGCTGGCCTG GACATCACAGATGTCTCCCTGCGGCTCATTATTCGCCATATGCCCCTGCTCTCGAAGCTCCAACTCAGTT ACTGTAACCACATCAATGACCAGTCCATCAACCTGCTCACTGCCGTCGGCACCACCACCCGAGACTCGCT GACAGAGGTCAACCTATCAGACTGTAATAAGGTAACTGACCTGTGCCTGTCCTTCTTCAAACGCTGTGGA AATATCTGTCATATTGACCTGAGGTACTGCAAGCAAGTCACCAAGGAAGGCTGTGAGCAATTCATAGCTG AAATGTCTGTGAGTGTCCAATTTGGGCAAGTGGAAGAGAAACTCCTGCAAAAACTAAGT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG217278 representing NM_001005866
Red=Cloning site Green=Tags(s) MRQLLRAIDRQRYDENEDLSDVEEIVSVRGFSLEEKLRSQLYQGDFVHAMEGKDFNYEYVQREALRVPLV FRDKDGLGIKMPDPDFTVRDVKLLVGSRRLVDVMDVNTQKGTEMSMSQFVRYYETPEAQRDKLYNVISLE FSHTKLEHLVKRPTVVDLVDWVDNMWPQHLKEKQTEATNALAEMKYPKVKKYCLMSVKGCFTDFHIDFGG TSVWYHVFRGGKIFWLIPPTLHNLALYEEWVLSGKQSDIFLGDRVERCQRIELKQGYTFFIPSGWIHAVY TPVDSLVFGGNILHSFNVPMQLRIYEIEDRTRVQPKFRYPFYYEMCWYVLERYVYCVTQRSYLTQEYQRE LMLIDAPRKTSVDGFSSDSWLDMEEESCEQQPQEEEEEEEDKEEEGDGADKTPKPPTDDPTSPTSTPPED QDSTGKKPKAPAIRFLKRTLSNESEESVKSTSMPTDDPKTPTGSPATEVSTKWTHLTEFELKGLKALVEK LESLPENKKCVPEGIEDPQALLEGVKNVLKEHVDDDPTLAITGVPVVSWPKKTAKNRVVGRPKGKLGPAS AVKLAANRTTAGARRRRTRCRKCEACLRTECGECHFCKDMKKFGGPGRMKQSCIMRQCIAPVLPHTAVCL VCGEAGKEDTVEEEEGKFNLMLMECSICNEIIHPGCLKIKESEGVVNDELPNCWECPKCNHAGKTGKQKR GPGFKYASNLPGSLLKEQKMNRDNKEGQEPAKRRSECEEAPRRRSDEHPKKVPADGILRRKSDDVHLRRK RKYEKPQELSGRKRASSLQTSPGSSSHLSPRPPLGSSLSPWWRSSLTYFQQQLKPGKEDKLFRKKRRSWK NAEDRLSLANKPLRRFKQEPEDDLPEAPPKTRESDQSRSSSPTAGPSTEGAEGPEEKKKVKMRRKRRLVN KELSKELSKELNHEIQKTESTLAHESQQPIKSEPESENDEPKRPLSHCERPHRFSKGLNGTPRELRHSLG PGLRSPPRVMSRPPPSASPPKCIQMERHVIRPPPISPPPDSLPLDDGAAHVMHREVWMAVFSYLSHRDLC VCMRVCRTWNRWCCDKRLWTRIDLNRCKSITPLMLSGIIRRQPVSLDLSWTNISKKQLSWLINRLPGLRD LVLSGCSWIAVSALCSSSCPLLRTLDVQWVEGLKDAQMRDLLSPPTDNRPGQMDNRSKLRNIVELRLAGL DITDVSLRLIIRHMPLLSKLQLSYCNHINDQSINLLTAVGTTTRDSLTEVNLSDCNKVTDLCLSFFKRCG NICHIDLRYCKQVTKEGCEQFIAEMSVSVQFGQVEEKLLQKLS TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001005866 |
ORF Size | 3909 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001005866.1, NP_001005866.1 |
RefSeq Size | 5026 bp |
RefSeq ORF | 3912 bp |
Locus ID | 30841 |
Gene Summary | The protein encoded by this gene is a H3K36-specific histone demethylase, which contains an N-terminal jumonji C domain, a CxxC zinc finger domain, a plant homeodomain finger, an F-box, and eight leucine-rich repeats. Amongst its demonstrated functions, this protein plays roles in the suppression of premature cellular senescence, leukemia maintenance and development, maintenance of mouse embryonic stem cell pluripotency, and induced pluripotent stem cell generation. Mice homozygous for a targeted deletion of the zinc finger domain display embryonic lethality with development ceasing at approximately 7 to 8 days post coitum, demonstrating an essential role in early development. A pseudogene of this gene is found on chromosome 4. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MR217278 | Kdm2b (Myc-DDK-tagged) - Mouse lysine (K)-specific demethylase 2B (Kdm2b), transcript variant 3 |
CNY 13,020.00 |
|
MR217278L3 | Lenti ORF clone of Kdm2b (Myc-DDK-tagged) - Mouse lysine (K)-specific demethylase 2B (Kdm2b), transcript variant 3 |
CNY 14,920.00 |
|
MR217278L4 | Lenti ORF clone of Kdm2b (mGFP-tagged) - Mouse lysine (K)-specific demethylase 2B (Kdm2b), transcript variant 3 |
CNY 14,920.00 |