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Wnk2 (NM_029361) Mouse Tagged ORF Clone
CAT＃: MG217367
- TrueORF®
Wnk2 (tGFP-tagged) - Mouse WNK lysine deficient protein kinase 2 (Wnk2), (10ug)
ORF Plasmid: DDK
"NM_029361" in other vectors (2)
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CNY 24,800.00
货期*
9周
规格
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经常一起买 (4)
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 1810073P09Rik; AW122246; ESTM15; mKIAA1760; X83337 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG217367 representing NM_029361
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACGGCGATGGCGGCCGCCGAGACGCCCCCGGCGCGCTGATGGAAGCCGGGCGCGGCACGGGGTCGG CGGGCATGGCGGAGCCGCGGGCGAGGGCGGCGCGGCTCGGGCCCCAGCGCTTCCTGCGGCGCAGCGTGGT GGAGTCGGACCAGGAGGAGCCGCCGGGCCTGGAGGCGGCCGAGACGCCGAGCGCGCAGCCCCCACAGCCT CTGCAGCGCAGGGTGCTTCTACTCTGCAAGACGCGCCGGCTCATCGCGGAGCGCGCCCGGGGCCGCCCCG CCGCGCCCGCGCCCGCCGCGCCCGCTGCGCCTCCCGGCTCCCCGAGTGTCCCCTCGGACCCTGGCCCGGA GCGAGCGGGCACGCAGGAGCCCAGCCCAGATCCCACCACCGCCTCAGCCGCAGCCGCGCAGGTCCCCGAC GGCGGCCCGAGGCAGGAAGAGGCGCCGGCCCCAACACAGGAGGACGCTGGAACCACCGAGGCAAAGCCGG AGCCCGGGCGCGCACGCAAGGATGAACCTGAAGAGGAGGAAGACGATGAGGACGACCTCAAGGCCGTGGC CACCTCTTTGGACGGCCGCTTCCTCAAGTTCGACATCGAGCTGGGCCGGGGCTCCTTCAAGACGGTCTAC AAGGGGCTGGACACGGAGACCTGGGTGGAGGTAGCATGGTGTGAACTGCAGGACCGGAAGCTCACCAAGC TTGAACGGCAGAGATTCAAAGAGGAGGCGGAGATGCTGAAAGGTCTACAGCACCCCAACATTGTGCGCTT CTATGACTTCTGGGAGTCCAGTGCCAAGGGCAAGCGATGTATCGTGCTGGTGACAGAGCTGATGACCTCG GGTACACTGAAGACGTACCTGAAGCGGTTCAAGGTGATGAAGCCGAAGGTACTGAGGAGCTGGTGCCGGC AGATCCTGAAGGGCCTGCTGTTCCTGCATACAAGGACACCCCCCATCATCCACCGTGACCTCAAGTGTGA CAACATCTTCATCACTGGGCCCACCGGCTCTGTGAAGATTGGTGACTTGGGTCTGGCCACCCTTAAAAGA GCGTCTTTTGCCAAAAGTGTGATAGGTACCCCCGAGTTCATGGCACCTGAAATGTACGAGGAACATTACG ACGAGTCGGTTGATGTCTACGCCTTCGGGATGTGCATGCTGGAAATGGCTACCTCAGAGTACCCCTATTC GGAGTGCCAGAACGCCGCCCAGATCTATCGCAAGGTCACCTGTGGCATCAAACCGGCCAGCTTCGAGAAG GTGCATGATCCGGAGATTAAGGAGATCATCGGGGAGTGCATCTGTAAGAACAAAGAGGAGAGGTATGAGA TTAAGGACCTGCTGAGCCATGCCTTCTTCGCGGAAGACACGGGTGTCCGGGTGGAACTTGCCGAGGAGGA CCATGGCAGGAAGTCTACCATAGCCCTGCGGCTTTGGGTGGAAGACCCCAAGAAGCTGAAGGGCAAGCCC AAAGACAATGGAGCCATAGAGTTTACCTTTGACTTGGAGAAGGAGACTCCCGACGAGGTGGCCCAGGAAA TGATCGATTCTGGATTTTTCCATGAGAGTGATGTGAAGATTGTGGCCAAGTCCATCCGTGACCGGGTGGC ACTGATTCAGTGGCGGCGTGAGAGGATCTGGCCTGCACTGCAGTCTCAGGAGCCAAAGGACTCTGGCAGC CCTGACAAGGCCAGGGGTCTGCCAGCACCCCTGCAGGTCCAGGTGACCTACCACGCACAGAGTGGGCAGC CTGGGCAGCCAGAACCTGAGGAGCCTGAGGCCGACCAGCATCTCCTGCCCCCTACCTTGCCAGCTAGCGT TACCTCCTTAGCCTCGGACAGCACCTTTGACAGTGGCCAGGGCTCTACCGTGTACTCGGACTCCCAGAGC AGCCAGCAGAGCATGGTGCTCAGCTCGCTTGTGGACACGGCCCCAACCCCAGCCTCGTGCGTGTGCAGCC CCCCTGTGAGTGAGGGGCCTGGCCTGACCCACAGCCTGCCTACGCTAGGGGCCTTCCAGCAGCCGGCCAC TGTGCCTGGCCTGTCTGTGGGCCCTGTGCCACCCCCTGCCCGCCCTCCACTCCTCCAGCAGCATTTTCCA GAATCTTCCATGAGCTTCACCCCTGTGCTGCCACCACCCAGCACCCCTGTGCCCACGGGCCCAAGCCAGC CAGCACCCCCAGTCCAGCAGCCTCTTCCAATGGCCCAACCACCTACCCTGCCACAGGTCCTGGCCCCACA GCCCATGGGCACTGTCCAGCCAGTACCCTCTCATTTGCCTCCATACCTGGCTCCAACCTCCCAGGTCGTG GCCCCTGCTCAGCTGAAGCCTCTCCAGATGCCACAGCCACCTCTGCAACCTCTTGCTCAAGTCCCTCCGC AGATGCCTCAGATGCCTGTGGTCCCCCCCATCACCCCCTTAACAGGACTCGATGGCCTCCCTCAGACCCT CACTGACCTGCCGGCCGCAAACGTGGCCCCCGTGCCACCACCTCAGTATTTCTCACCTGCTGTGATCTTG CCAAGCCTCACAACACCCCTGCCCACATCACCGGCCCTGCCTATGCAGGCTGTCAAGTTGCCTCATCCCC CTGGAACACCCCTGGCTGTGCCCTGTCAGACCATCGTGCCAAATGCACCAGCTGCTATCCCCCTGTTGGC CGTGGCCCCGCAGGGTGTGGCTGCACTGTCTATCCATCCAGCGGTGGCCCAGATCCCAGCCCAGCCTGTG TACCCAGCAGCCTTTCCACAGATGGTACCTGGAGACATCCCACCCTCACCCCACCACACGGTGCAGAGCT TACGGGCCACCCCTCCACAGCTGGCCTCACCTGTGCCTCCTCAGCCTGTCCAGCCCAGTGTCATTCATCT ACCTGAGCAGGCTGCTCCAACTGCTGCCTCAGGGACCCAGGTCCTGCTGGGCCACCCTCCTTCATACACC GCGGACGTTGCTGCTCCAGTCTCTGCTGTGTCCCTGCCGCCTGCTGTCCTTTCCCCTCCTCTGCCAGATA CTCTGCTGCCTACTGTCCCTGACCTCCTGCCCAAGGTCCCCAGCTCTCTGGCCCCTACAGTGGTCGCAGC CTCTCAGAGTGCGCCCGCACAGACCTCTTCACTCCTCCTGCCAACAAACCCACCCCTGCCCACTGGGCCT GCAGTTGCTGGCCCCTGCCCTGCAGTCCAGCTGATGGTGGAAGTGGCTCAAGAGGAGCAGGTCTCACAAG ACAAACCCCCTGGCCCCCCACAGAGCTCCGAGAGCTTTGGAGGTTCTGATGTCACCTCTGGAAGAGATCT GAGTGACAGCTGTGAAGGCACCTTTGGAGGGGGCAGGCTGGAGGGAAGGACCGCCCGGAAACACCACCGT AGGTCCACTCGTGCACGCTCGCGGCAGGAGAGGGCCAGCCGGCCCCGGCTCACTATCCTAAATGTGTGTA ACACGGGGGACAAGATGGTGGAGTGTCAGCTGGAAACGCATAACCATAAGATGGTGACCTTCAAGTTTGA CCTCGATGGGGACGCTCCGGATGAGATTGCCACGTACATGGTAGAGCATGACTTTATCCTGCCCGCTGAG CGAGAGACCTTCATCGAGCAGATGAAGGATGTCATGGACAAGGCAGAAGACATGCTCAGTGAGGACACAG ATGCTGACCATGGCTCTGATACAGGGACCAGCCCTCCACACCTGGGTACCTGTGGTCTGGCCACGGGGGA GGAGAACAGACAGTCCCAAGCCAATGCCCCTGTCTACCAGCAGAATGTCTTACACACGGGAAAGAGGTGG TTTATCATCTGTCCAGTGGCTGAACACCCAGCAACAGACACTTCTGAATCATCACCCCCACTTCCTCTAA GCTCCTTGCAGCCAGAAGCCAGCCAAGACCCAGCGCCCTATCCAGACCAGCTGTCCTTGACGGACAAACC TAGTTTCCCAGCTGCTCAGCAGCTCCTGAGCCAGGCAGGCTCCAGCAACCCTCCTGGTGGAGCATCAGCC CCCTTGGCCCCGTCTTCCCCTCCTGTGACTACTGTAATCCCGGCAGCACCAGCCACCAGCACCGTGCCAG AGTCAGCAGCAGGGACTGCCATGCAGGCAGGAGGTCCAGGGACTCATCAAGGACCAGCCAGTGTCCATGA GACCCTCCAGCCACTGGCCGAGACACGCTCTGCCCAGTGTACTGCCCAGCCCCTTAGTACTGGCCAAGGA CCTTGCACCCCAGCTCTGGAAGCCTCTCGCTGCTCCACAGGTCTGGGGGAGCCCATCTCAACCAGGGAGG TCAGCACTCAAGGAGAGCCCCTGCCTGCTTCAGTGCCAGAACCTAGCCCTCCCACTGGGGCCACACAATC TGTACCGGGTCAGCCACCACCCCCACTGCCCATCACAGTAGGGGCTATCAGTCTGGCAGCTCCCCAGCTC CCCAGTCCCCCCCTGGGACCTACTGCTCCCCCACCACCCCCCTCAGCCTTGGAATCTGATGGGGAAGGGC CGCCCCCTAGGGTGGGCTTCGTGGACAACACCATCAAGAGTCTGGATGAGAAACTGCGGACTCTGCTCTA CCAGGAGCACGTGCCCACCTCCTCAGCCTCAGCTGGGACCCCCATGGAGGCAAGCGATAGAGACTTCACC CTGGAGCCCCTGCGAGGAGACCTTCCCTCAGCCTTGAGTGACAAGACCCCAAGCCTCACCCAGCAGACCC AGCCCTCATTGGAAAAGTCTGAAACGGCCCCTGCAGGATGGGCCCTGGCCCAGAGGGAACAGGGTGCATC TTCTCCAATGACAGCAGAGTCATCTTCCAGCAATACACTGGGCTGTGACAGTGACGCAGGGCAGGTGGCC TCGGACTCCTCCACAGCACCCAGTGTCCCCCAGGATGCCTCTGGTTCTTCCGTCCCTACACACATGGATC CCAAAGATCAGAACAGTAGTGTTCCCAGAGAAGCCTTGGCAGCACCCATGCAAAGTGGCCCAGGGTCCTT CACAGTGGGCAGCCCAGCCCAGCTGCGTGGCGCCCGGGACTCCGGGTCTCCTCATAAGCGGCCAGGGCAG CAGGACAACAGTTCTCCAGCCAAAACTGTAGGGCGCTTTTCCGTGGTCAGCACACAGGACGAGTGGACTC TGGCGTCACCTCACAGCCTGCGGTACTCTGCCCCACCCGATGTCTACCTGGATGAGATCCCCTCTAGCCC TGAGGTGAAGCTGGCAGTGCGGCGGGTACAGACAGCCTCCTCCATCGAGGTTGGCGTTGAGGAACCTGCC TCTAGTGACTCTGGGGATGAACGCCCAAGAAGGAGGTCCCAGGTGCAGAAGCAGTCCTCCCTGCCGGGTA CTGGTGGTGTGGCAAGTGACTTTGTGAAGAAGGCCACTGCCTTCCTGCACAGGTCTTCAAGGGCTGGCTC GCTGGGACCTGAGACACCCAGTAGGGCAGGTGTGAAGGTCCCTACCATCAGCATCACCTCCTTCCACTCC CAGTCATCCTACATCAGCAGTGACAATGACTCAGAGTTTGAGGATGCAGACATAAAGAAGGAACTGCGCA GTCTGCGAGAAAAGCACCTGAAGGAAATCTCGGAGCTACAGAGCCAGCAGAAGCAGGAGATCGAAGCCCT GTACCGACGCTTGGGCAAGCCGCTCCCTCCCAATGTGGGGTTCTTCCACACTGCACCCCCTATGGGCCGC AGGAGGAAAACCAGCAAGAGCAAATTGAAGGCTGGCAAGCTGCTGAACCCCTTGGTGCAACAGCTCAAGG TCGTGGCCTCCAGCACAGGGTCAGCCCTGAAGCGTCTCTGCCTAGGCAAAGAACACAGCAGTAGGTCTTC CACCAGCAGCCTGGCCCCAGGCCCTGAGCCAGGTCCCCAGCCCACCCTGCACGTCCAGGCGCAGGTGAAC AACAGCAACAACAAGAAAGGCACCTTCACGGACGACCTGCACAAGTTGGTGGACGAGTGGACAACGAAGA CCGTGGGGGCAGCGCAAGTGAAGCCCACGCTCAACCAGCTGAAGCAGACGCAGAAGCTGCACGACATGGA GGCCTCTGGAGACGCTCGGGCTACATCTGTGCCTCGAGCAGCAGTGGGGGCATCGTGTCTGGCCCCGGCC CCTGGCCCTCTGTCCACCACAGCCACTCCTGGAGCCACTCCAGCCCTGCCTGTGCCCATACCAGATCCTG AGAGTGAAAAGCCCGAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG217367 representing NM_029361
Red=Cloning site Green=Tags(s) MDGDGGRRDAPGALMEAGRGTGSAGMAEPRARAARLGPQRFLRRSVVESDQEEPPGLEAAETPSAQPPQP LQRRVLLLCKTRRLIAERARGRPAAPAPAAPAAPPGSPSVPSDPGPERAGTQEPSPDPTTASAAAAQVPD GGPRQEEAPAPTQEDAGTTEAKPEPGRARKDEPEEEEDDEDDLKAVATSLDGRFLKFDIELGRGSFKTVY KGLDTETWVEVAWCELQDRKLTKLERQRFKEEAEMLKGLQHPNIVRFYDFWESSAKGKRCIVLVTELMTS GTLKTYLKRFKVMKPKVLRSWCRQILKGLLFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKR ASFAKSVIGTPEFMAPEMYEEHYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRKVTCGIKPASFEK VHDPEIKEIIGECICKNKEERYEIKDLLSHAFFAEDTGVRVELAEEDHGRKSTIALRLWVEDPKKLKGKP KDNGAIEFTFDLEKETPDEVAQEMIDSGFFHESDVKIVAKSIRDRVALIQWRRERIWPALQSQEPKDSGS PDKARGLPAPLQVQVTYHAQSGQPGQPEPEEPEADQHLLPPTLPASVTSLASDSTFDSGQGSTVYSDSQS SQQSMVLSSLVDTAPTPASCVCSPPVSEGPGLTHSLPTLGAFQQPATVPGLSVGPVPPPARPPLLQQHFP ESSMSFTPVLPPPSTPVPTGPSQPAPPVQQPLPMAQPPTLPQVLAPQPMGTVQPVPSHLPPYLAPTSQVV APAQLKPLQMPQPPLQPLAQVPPQMPQMPVVPPITPLTGLDGLPQTLTDLPAANVAPVPPPQYFSPAVIL PSLTTPLPTSPALPMQAVKLPHPPGTPLAVPCQTIVPNAPAAIPLLAVAPQGVAALSIHPAVAQIPAQPV YPAAFPQMVPGDIPPSPHHTVQSLRATPPQLASPVPPQPVQPSVIHLPEQAAPTAASGTQVLLGHPPSYT ADVAAPVSAVSLPPAVLSPPLPDTLLPTVPDLLPKVPSSLAPTVVAASQSAPAQTSSLLLPTNPPLPTGP AVAGPCPAVQLMVEVAQEEQVSQDKPPGPPQSSESFGGSDVTSGRDLSDSCEGTFGGGRLEGRTARKHHR RSTRARSRQERASRPRLTILNVCNTGDKMVECQLETHNHKMVTFKFDLDGDAPDEIATYMVEHDFILPAE RETFIEQMKDVMDKAEDMLSEDTDADHGSDTGTSPPHLGTCGLATGEENRQSQANAPVYQQNVLHTGKRW FIICPVAEHPATDTSESSPPLPLSSLQPEASQDPAPYPDQLSLTDKPSFPAAQQLLSQAGSSNPPGGASA PLAPSSPPVTTVIPAAPATSTVPESAAGTAMQAGGPGTHQGPASVHETLQPLAETRSAQCTAQPLSTGQG PCTPALEASRCSTGLGEPISTREVSTQGEPLPASVPEPSPPTGATQSVPGQPPPPLPITVGAISLAAPQL PSPPLGPTAPPPPPSALESDGEGPPPRVGFVDNTIKSLDEKLRTLLYQEHVPTSSASAGTPMEASDRDFT LEPLRGDLPSALSDKTPSLTQQTQPSLEKSETAPAGWALAQREQGASSPMTAESSSSNTLGCDSDAGQVA SDSSTAPSVPQDASGSSVPTHMDPKDQNSSVPREALAAPMQSGPGSFTVGSPAQLRGARDSGSPHKRPGQ QDNSSPAKTVGRFSVVSTQDEWTLASPHSLRYSAPPDVYLDEIPSSPEVKLAVRRVQTASSIEVGVEEPA SSDSGDERPRRRSQVQKQSSLPGTGGVASDFVKKATAFLHRSSRAGSLGPETPSRAGVKVPTISITSFHS QSSYISSDNDSEFEDADIKKELRSLREKHLKEISELQSQQKQEIEALYRRLGKPLPPNVGFFHTAPPMGR RRKTSKSKLKAGKLLNPLVQQLKVVASSTGSALKRLCLGKEHSSRSSTSSLAPGPEPGPQPTLHVQAQVN NSNNKKGTFTDDLHKLVDEWTTKTVGAAQVKPTLNQLKQTQKLHDMEASGDARATSVPRAAVGASCLAPA PGPLSTTATPGATPALPVPIPDPESEKPD TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_029361 |
| ORF Size | 6177 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_029361.4, NP_083637.2 |
| RefSeq Size | 6618 bp |
| RefSeq ORF | 6180 bp |
| Locus ID | 75607 |
| UniProt ID | Q3UH66 |
| Gene Summary | Serine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival, and proliferation. Acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively. Activates SLC12A2, SCNN1A, SCNN1B, SCNN1D and SGK1 and inhibits SLC12A5. Negatively regulates the EGF-induced activation of the ERK/MAPK-pathway and the downstream cell cycle progression. Affects MAPK3/MAPK1 activity by modulating the activity of MAP2K1 and this modulation depends on phosphorylation of MAP2K1 by PAK1. WNK2 acts by interfering with the activity of PAK1 by controlling the balance of the activity of upstream regulators of PAK1 activity, RHOA and RAC1, which display reciprocal activity.[UniProtKB/Swiss-Prot Function] |
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