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Pla2g4e (NM_177845) Mouse Tagged ORF Clone
CAT＃: MG217443
- TrueORF®
Pla2g4e (tGFP-tagged) - Mouse phospholipase A2 group IVE (Pla2g4e), (10ug)
ORF Plasmid: DDK
"NM_177845" in other vectors (4)
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CNY 11,208.00
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Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 2310026J01Rik; C230096D22; Pla2epsilon |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG217443 representing NM_177845
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGTCTATTCCACACTCCGATGAAGCAGACGTGGCTGGGATGACCCACGCCTCAGAAGGCCACCATG GCCTAGGGACCAGCATGCTTGTCCCACAGAACCCACAAGGGTCAGAAGACAGCAAGCCAGCAAGAAACTG CAGTGGATTTGAAGATGCACAGGACCCACAGACTGCTGTGCCCTCACCTTTACTTCCCATGGCTTCTTGC AGCTCTCAGGAGGGGTCATCTCCATGCCATCTGTTGACAGTGAGGATCATTGGCATGAAAAACGTCCGGC AGGCTGATATACTGAGTCAGACAGACTGCTTTGTGACCCTCTGGCTGCCTACTGCCTCTCAGAAGAAGCT GAAGACCAGAACCATCTCCAACTGCCTACACCCAGAGTGGGACGAAAGCTTCACCTTTCAGATCCAGACT CAAGTAAAGAATGTGCTAGAGCTGAGCGTCTGTGACGAAGACACCCTGACACAAAATGACCATCTCTTGA CAGTCCTCTATGACCTCTCTAAGCTCTGCCTCCGGAATAAAACCCATGTGAAGTTCCCACTCAACCCAGA GGGCATGGAAGAACTGGAGGTGGAGTTCCTACTCGAAGAGAATTTCTCCTCATCAGAGACCCTCATCACC AACGGCGTGCTGGTGTCTCGCCAAGTCTCTTGCCTGGAGGTTCATGCAGAATCCAGGAGGCCGAGGAAGA GGAAGAAAAACAAAGACCTTCTGGTGATGGTGACAGACTCCTTCGAGAACACCCAGCGTGTCCCGCCTTG CCGGGAGCCCTGCTACCCCAATTCTGCCTGCTTCCACTACCCCAAGTACTCCCAGCCACAGCTTTACGCA GAGGCGCCTAAGAGCCACTGTAACTTTAGGCTTTGCTGCTGCGGAACACACAGGAATGACCCTGTCTGCC AGCCCCTCAATTGCCTTTCTGATGGCCAGGTGACAACCCTGCCTGTGGGAGAGAACTATGAGCTACACAT GAAGTCCTCACCCTGCTCTGACACACTGGATGTGCGGCTTGGATTCAACCTGTGCCAGGAAGAGGTGGAG TTTGTGCAGAAGCGGAAGATGGTGGTGGCCAAGACACTAAGTCAGATGCTGCAGCTGGAGGAAGGCCTGC ATGAGGATGAGGTACCGATAATAGCCATCATGGCCACAGGAGGTGGCACAAGGTCTATGGTCTCCTTGTA TGGCCACCTGCTGGGGTTGCAGAAGCTGAACTTTCTGGACACTTCTACTTACATCACCGGCTTGTCAGGT GCAACCTGGACTATGGCTACCTTGTACAGTGATCCTGAGTGGTCCTCCAAAAACCTGGAGACTGTTGTCT TTGAGGCCCGGAGACATGTTGTCAAAGACAAGATGCCTGCCCTGTTCCCAGATCAGCTCTACAAATGGCG AGAGGACCTCCAAAAGCATAGCCAGGAGGGCTATAAGACCACGTTTACAGACTTTTGGGGCAAGCTGATC GAGTACAGTCTGGGAGATAAAAAAAACGAATGCAAGCTGTCAGATCAGCGAGCTGCTCTGTGCCGGGGAC AGAACCCTCTGCCCATCTACCTCACCATCAATGTCAAGGATGATGTAAGCAACCAGGATTTCAGAGAATG GTTCGAGTTCTCCCCCTACGAGGTGGGCATGCAGAAGTACGGAGCCTTCATCCCCAGCGAGTTATTTGGC TCCGAGTTCTTCATGGGGCGGCTGATGAAGAGGATTCCTGAGCCGGAGATGTGCTACATGCTAGGGTTGT GGAGTAGCATCTTTTCCCTGAACCTGCTTGATGCCTGGAATTTGTCTCACACCTCAGAGGAGTTTTTCTA TAGGTGGACAAGGGAGAGACTGCATGACATCGAAGATGATCCCATCCTGCCTGAAATCCCTAGGTGTGAC GATAACCCCCTAGAGACCACAGTAGTGATCCCAACGACATGGCTGTCCAACACCTTCCGAGAAATCCTCA CACGCAGGCCCTTCGTGTCTGAGTTCCACAACTTCCTGTACGGGATGCAGCTGCATACTGACTACTTACA GAACAGACAGTTCTCTATGTGGAAAGACACAGTACTGGACACCTTCCCAAACCAGCTGACACAGTTTGCA AAACACCTGAACCTGCTGGACACTGCGTTCTTTGTCAACTCCAGCTACGCACCCCTCCTCAGGCCAGAGA GAAAAGTCGACCTTATCATCCACCTCAATTACTGCGCAGGATCCCAGACAAAGCCCCTGAAACAAACCTG TGAGTACTGTACCGAGCAGAAGATCCCCTTCCCCAGCTTCTCCATCCTGGAAGATGACAACAGTCTCAAG GAGTGCTACGTGATGGAGAATCCCCAGGAGCCCGACGCCCCCATCGTGGCTTACTTCCCACTCATCAGTG ACACCTTCCAGAAGTACAAGGCTCCAGGTGTAGAGCGAAGTCCTGACGAGCTGGAACTGGGCCAGCTGAA CATCTATGGACCAAAGTCTCCCTATGCCACCAAGGAGCTGACGTACACAGAGGCCGCCTTCGACAAGCTG GTGAAGCTCTCAGAATATAATATCCTCAATAACAGAGATAAGCTCATTCAGGCCTTGAGACTAGCAATGG AGAAGAAACGCATGAGGAGCCAGTGTCCCTCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG217443 representing NM_177845
Red=Cloning site Green=Tags(s) MQSIPHSDEADVAGMTHASEGHHGLGTSMLVPQNPQGSEDSKPARNCSGFEDAQDPQTAVPSPLLPMASC SSQEGSSPCHLLTVRIIGMKNVRQADILSQTDCFVTLWLPTASQKKLKTRTISNCLHPEWDESFTFQIQT QVKNVLELSVCDEDTLTQNDHLLTVLYDLSKLCLRNKTHVKFPLNPEGMEELEVEFLLEENFSSSETLIT NGVLVSRQVSCLEVHAESRRPRKRKKNKDLLVMVTDSFENTQRVPPCREPCYPNSACFHYPKYSQPQLYA EAPKSHCNFRLCCCGTHRNDPVCQPLNCLSDGQVTTLPVGENYELHMKSSPCSDTLDVRLGFNLCQEEVE FVQKRKMVVAKTLSQMLQLEEGLHEDEVPIIAIMATGGGTRSMVSLYGHLLGLQKLNFLDTSTYITGLSG ATWTMATLYSDPEWSSKNLETVVFEARRHVVKDKMPALFPDQLYKWREDLQKHSQEGYKTTFTDFWGKLI EYSLGDKKNECKLSDQRAALCRGQNPLPIYLTINVKDDVSNQDFREWFEFSPYEVGMQKYGAFIPSELFG SEFFMGRLMKRIPEPEMCYMLGLWSSIFSLNLLDAWNLSHTSEEFFYRWTRERLHDIEDDPILPEIPRCD DNPLETTVVIPTTWLSNTFREILTRRPFVSEFHNFLYGMQLHTDYLQNRQFSMWKDTVLDTFPNQLTQFA KHLNLLDTAFFVNSSYAPLLRPERKVDLIIHLNYCAGSQTKPLKQTCEYCTEQKIPFPSFSILEDDNSLK ECYVMENPQEPDAPIVAYFPLISDTFQKYKAPGVERSPDELELGQLNIYGPKSPYATKELTYTEAAFDKL VKLSEYNILNNRDKLIQALRLAMEKKRMRSQCPS TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_177845 |
| ORF Size | 2625 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_177845.4, NP_808513.2 |
| RefSeq Size | 4477 bp |
| RefSeq ORF | 2628 bp |
| Locus ID | 329502 |
| UniProt ID | Q50L42 |
| Gene Summary | Calcium-dependent phospholipase A2 that selectively hydrolyzes glycerophospholipids in the sn-2 position.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC222357 | Pla2g4e (untagged) - Mouse phospholipase A2, group IVE (Pla2g4e), (10ug) |
CNY 11,210.00 |
|
| MR217443 | Pla2g4e (Myc-DDK-tagged) - Mouse phospholipase A2, group IVE (Pla2g4e) |
CNY 9,608.00 |
|
| MR217443L3 | Lenti ORF clone of Pla2g4e (Myc-DDK-tagged) - Mouse phospholipase A2, group IVE (Pla2g4e) |
CNY 10,640.00 |
|
| MR217443L4 | Lenti ORF clone of Pla2g4e (mGFP-tagged) - Mouse phospholipase A2, group IVE (Pla2g4e) |
CNY 10,640.00 |
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