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Hecw2 (NM_001001883) Mouse Tagged ORF Clone
CAT＃: MG217597
- TrueORF®
Hecw2 (tGFP-tagged) - Mouse HECT C2 and WW domain containing E3 ubiquitin protein ligase 2 (Hecw2) transcript variant 1, (10ug)
ORF Plasmid: DDK
"NM_001001883" in other vectors (4)
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CNY 17,390.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | A730039N16Rik; D030049F17Rik; Gm971; mKIAA1301; Nedl2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG217597 representing NM_001001883
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTAGCTCAGCCCGGGAGCATTTGCTTTTTGTGCGGCGTCGAAATCCACAGATGCGGTACACACTGA GCCCAGAGAACCTCCAGAGCCTGGCAGCCCAGAACTCAATGCCCGAGAACATGGCCCTACAGCGGGCCAA CAGTGACACGGATCTGGTGACTTCTGAGAGCCGCTCCAGCCTGACTGCCAGCATGTACGAATACACGCTG GGGCAAGCCCAGAACCTCATTATCTTCTGGGACATTAAGGAAGAGGTTGACCCCAGCGATTGGATAGGAC TCTATCACATAGATGAGAATTCTCCAGCCAACTTCTGGGACTCTAAAAACAGGGGTGTGACTGGAACCCA AAAAGGACAAATTGTATGGCGAATTGAGCCTGGGCCCTACTTCATGGAACCGGAGATCAAAATCTGTTTC AAATACTACCATGGTATTAGTGGAGCCCTGCGAGCCACAACCCCCTGTATTACCGTGAAGAACCCAGCTG TGATGATGGGGGCCGAAGGCATGGAAGGAGGTGCTTCAGGAAGCCTGCACTCTAGGAAACTCGTCAGCTT CACATTGTCAGATCTCAGGGCAGTTGGGCTCAAGAAAGGGATGTTCTTCAATCCTGACCCTTATCTTAAG ATGTCCATTCAGCCAGGGAAGAAGAGCAGTTTTCCTACCTGCGCCCACCATGGGCAGGAGAGAAGGTCCA CTATCATCAGCAACACCACCAATCCGATCTGGCACAGAGAGAAATACTCCTTTTTTGCACTTTTGACTGA TGTCTTAGAAATTGAAATCAAAGACAAATTTGCCAAGAGCCGGCCCATCATCAAACGCTTCCTGGGGAAA CTAACCATTCCAGTGCAGAGGCTGCTGGAGCGGCAGGCTGGGGACCAAATGCTCAGCTACAACCTTGGCA GACGGCTCCCAGCTGACCACGTGAGTGGGTACCTCCAGTTTAAAGTGGAAGTTACATCTTCTGCTCACGA AGATGCTTCTCCAGAGGCTGTCGGCACCATCCTCGGAGTCCACACTGTGAATGGAGACCTGGGCAGCCCT TCCGATGAGGAGGATATGCCAGGCAGCCATCATGACAGTACTATCTGTGCTAATGGGCCAGTGTCCGAGG ACAGTGTTGCTGATGGCACCCCTAAGCATTCTTTCAGGACTAGCTCTACCCTGGAGATCGACACGGAGGA CTTGATCTCTACTTCTTCTAGAAATTCACCCCCCAGGGGCCGCCAGGATTCCCTCAATGATTACTTAGAT GCTATTGAACACAATGGCCCCGCCAGGCCAGGGGCAGCCTCCTCCTCTGAGCGGTCCATGGGAGCCTCTC CTAAACTGAGGAGTAGTTTTCCCACTGACACAAGACTCAATGCGATGCTTCATATTGACTCAGACGAAGA AGACCATGAGTTTCAGCAAGACTTGGGCTACCCATCTTCTTTGGAGGAGGAGGGAGGCTTGATCATGTGC AGCAGGGCGTCGAGAATTGATGATGGGAGCCTGACATCTCAGACCAAACCAGAGGATGACAACCCTGTGG AGAATGAGGATGCCTCTATACATGAAACTGCATCCTTAGAAGAGAGGCTGGAGAATCTTCCAGAGGTTGC AGATGGCTCCTTGCCATCAAGTACAGCCCCTGATGAAAATGAAGCCAACCTGGAGCCTCAACCCAGTGCA GACCAGGGTAGCACTGAATTGTGTAGCTCTCAAGAAGTAGATCAGCCCACAAGTGGTGCAGATGCAGGAG CTTCTGACACATCTGGAGGGAGCCGCAGAGCTGCCAGTGAGACCGAGTCCCTGGACCAGGGATCTGAACC TTCCCAGGTGTCCTCTGAGACAGAACCCAGTGACCCTGCAAGGACAGAGAGTGTGAGTGAGGCCAGTACC AGACCTGAGGGGGAGAGTGACCCGGAAGGCGCAGACAGCTCATGCAATGAGAGCGTGACTACACAGCTGT CCTCCGTGGAGACACGGTGCTCATCTCTAGAGAGTGCGCGATTTCCAGAAACCCCCGCCTTTTCTTCTCA GGAGGAAGAAGATGGGGCCTGTGCAGCTGAGCCCACCAGCAGTGGCCCCGCAGAGGGGTCGCAGGAGTCC GTCTGCACTCCTAGTTCTTTACCTGCTGTGCAGGTGCCCAGCAGGGAAGAGGAAGGGTCAGCGGCTGAAG CTGCTGCTTTATCTGAGCAGGGGGAGCTAGGGGAAGTCTGGCAGAGGAGGGGGAGTCTTGAGGGAGCAGC CGCTGCTGCTCCTGCTGCTGCTGCCACTGATAGCCAGCCCCAGGAAGATGGGGATGCTGGGGATGCCCAA GGTGCCTGCGAAGGGGCCACTGCCCAGGAGGAGGGCGCCACTGGAGGTTCTCAAACCAATGGCCACCAGC CCTTGAGGTCACTACCTTCCGTGCGCCAGGACGTCAGCCGGTACCAGAGGGTGGATGAGGCTCTCCCACC AAACTGGGAGGCACGGATTGACAGCCACGGCAGGATCTTCTATGTGGACCATGTAAACAGAACCACAACG TGGCAGCGGCCCACAGCTCCCCCTGCCCCACAGGTCCTGCAGCGATCTAACTCCATACAGCAAATGGAGC AGCTGAACCGACGTTATCAGAGCATCCGCAGAACCATGACTAATGAGAGGCCTGAGGAAAATACCAGTGC TATCGATGGGGCAGGGGAGGAAGCCGACTTTCACCAAGCCAGCGCAGACTTCCGTCGGGAGAACGTCCTG CCTCACTCTACCTCCAGATCCAGGCTCACGCTGCTGTTACAGTCACCCCCCGTGAAGTTCCTCATCAGCC CAGAGTTCTTCACCGTGCTGCATTCTAACCCTAGTGCCTACCGCATGTTTACAAACAACACGTGTTTGAA GCACATGATCACCAAAGTCCGGCGGGACACCCACCACTTTGAGCGCTATCAGCATAACAGGGACCTCGTG GGATTCCTCAACATGTTTGCTAACAAACAGCTGGAACTACCAAGGGGCTGGGAGATGAAACATGATCATC AGGGCAAGGCGTTTTTTGTCGACCACAACTCCCGGACCACCACGTTCATTGATCCTCGGCTCCCACTTCA GAGCAGTAGGCCCACAAGTGCATTGGTTCATCGACAACACCTGACGAGGCAACGCAGCCACAGTGCGGGT GAGGTAGGAGAAGACTCTCGGCACGCAGGACCACCAGTTCTTCCCAGGCCATCCAGTACATTCAATACGG TCAGTAGGCCGCAGTACCAGGACATGGTTCCAGTGGCCTACAATGACAAAATTGTTGCGTTTTTGCGTCA ACCCAACATCCTTGAAATTCTGCAAGAGCGTCAACCTGATCTTGCCAGAAACCACTCACTCAGGGAGAAG ATCCAATTTATCCGAACGGAAGGGACCCCTGGATTGGTGCGGCTCTCAAGCGATGCAGACCTTGTCATGT TACTGAGCTTATTCGAGGAAGAGATAATGTCATATGTGCCTCCTCATGCCTTACTCCACCCCAGCTACTG TCAGTCCCCGCGTGGCTCCCCCGTATCATCTCCCCAGAACTCACCAGGTACTCAGCGTGCCAATGCCCGG GCTCCAGCCCCTTACAAGAGAGATTTTGAAGCCAAACTAAGGAACTTTTACAGGAAGTTAGAAACTAAAG GATACGGACAAGGCCCAGGAAAATTAAAGCTAATTATCCGAAGAGATCACTTGCTGGAAGATGCTTTCAA TCAGATCATGGGCTACTCCAGAAAGGACCTGCAGAGGAATAAACTGTACGTCACCTTTGTCGGGGAGGAA GGGTTGGATTACAGTGGGCCATCCAGAGAGTTCTTCTTCCTGGTATCCCGAGAGCTCTTTAACCCCTATT ACGGCTTATTTGAATATTCAGCCAATGACACATATACAGTGCAAATAAGTCCCATGTCTGCCTTTGTAGA CAATCATCATGAATGGTTCCGGTTCAGCGGGAGGATCCTCGGCCTTGCGCTCATACACCAGTACTTGCTG GACGCCTTCTTCACGCGACCCTTCTATAAGGCTCTTCTCAGAATCCTGTGTGACCTGAGTGATCTGGAAT ACCTTGATGAGGAGTTCCACCAGAGCCTTCAGTGGATGAAAGACAATGACATCCATGACATCCTGGACCT CACGTTCACTGTGAATGAAGAAGTTTTTGGGCAGATTACTGAACGAGAGTTAAAACCAGGGGGAGCCAAT ATTCCAGTCACAGAGAAGAACAAGAAGGAGTACATCGAGAGAATGGTGAAGTGGAGGATCGAGAGGGGTG TGGTGCAGCAGACAGAGAGCCTAGTTCGTGGTTTCTACGAGGTAGTAGATGCCAGGCTAGTGTCTGTCTT TGATGCGAGAGAGCTGGAACTGGTCATTGCCGGCACAGCTGAGATAGATCTCAATGACTGGAGAAACAAC ACTGAATACAGAGGCGGGTACCATGACAATCACATTGTAATTCGGTGGTTCTGGGCTGCAGTGGAAAGAT TCAACAATGAACAACGACTAAGGTTGTTACAGTTTGTTACAGGCACATCCAGCATTCCCTATGAAGGATT CGCTTCTCTCCGAGGGAGTAATGGTCCAAGAAGATTCTGTGTGGAAAAATGGGGAAAAATCACCGCTCTT CCCAGAGCCCACACTTGTTTTAACCGCCTGGATCTGCCTCCATACCCTTCCTTTTCCATGCTTTATGAAA AACTCTTGACAGCAGTTGAAGAGACCAGTACCTTTGGACTTGAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG217597 representing NM_001001883
Red=Cloning site Green=Tags(s) MASSAREHLLFVRRRNPQMRYTLSPENLQSLAAQNSMPENMALQRANSDTDLVTSESRSSLTASMYEYTL GQAQNLIIFWDIKEEVDPSDWIGLYHIDENSPANFWDSKNRGVTGTQKGQIVWRIEPGPYFMEPEIKICF KYYHGISGALRATTPCITVKNPAVMMGAEGMEGGASGSLHSRKLVSFTLSDLRAVGLKKGMFFNPDPYLK MSIQPGKKSSFPTCAHHGQERRSTIISNTTNPIWHREKYSFFALLTDVLEIEIKDKFAKSRPIIKRFLGK LTIPVQRLLERQAGDQMLSYNLGRRLPADHVSGYLQFKVEVTSSAHEDASPEAVGTILGVHTVNGDLGSP SDEEDMPGSHHDSTICANGPVSEDSVADGTPKHSFRTSSTLEIDTEDLISTSSRNSPPRGRQDSLNDYLD AIEHNGPARPGAASSSERSMGASPKLRSSFPTDTRLNAMLHIDSDEEDHEFQQDLGYPSSLEEEGGLIMC SRASRIDDGSLTSQTKPEDDNPVENEDASIHETASLEERLENLPEVADGSLPSSTAPDENEANLEPQPSA DQGSTELCSSQEVDQPTSGADAGASDTSGGSRRAASETESLDQGSEPSQVSSETEPSDPARTESVSEAST RPEGESDPEGADSSCNESVTTQLSSVETRCSSLESARFPETPAFSSQEEEDGACAAEPTSSGPAEGSQES VCTPSSLPAVQVPSREEEGSAAEAAALSEQGELGEVWQRRGSLEGAAAAAPAAAATDSQPQEDGDAGDAQ GACEGATAQEEGATGGSQTNGHQPLRSLPSVRQDVSRYQRVDEALPPNWEARIDSHGRIFYVDHVNRTTT WQRPTAPPAPQVLQRSNSIQQMEQLNRRYQSIRRTMTNERPEENTSAIDGAGEEADFHQASADFRRENVL PHSTSRSRLTLLLQSPPVKFLISPEFFTVLHSNPSAYRMFTNNTCLKHMITKVRRDTHHFERYQHNRDLV GFLNMFANKQLELPRGWEMKHDHQGKAFFVDHNSRTTTFIDPRLPLQSSRPTSALVHRQHLTRQRSHSAG EVGEDSRHAGPPVLPRPSSTFNTVSRPQYQDMVPVAYNDKIVAFLRQPNILEILQERQPDLARNHSLREK IQFIRTEGTPGLVRLSSDADLVMLLSLFEEEIMSYVPPHALLHPSYCQSPRGSPVSSPQNSPGTQRANAR APAPYKRDFEAKLRNFYRKLETKGYGQGPGKLKLIIRRDHLLEDAFNQIMGYSRKDLQRNKLYVTFVGEE GLDYSGPSREFFFLVSRELFNPYYGLFEYSANDTYTVQISPMSAFVDNHHEWFRFSGRILGLALIHQYLL DAFFTRPFYKALLRILCDLSDLEYLDEEFHQSLQWMKDNDIHDILDLTFTVNEEVFGQITERELKPGGAN IPVTEKNKKEYIERMVKWRIERGVVQQTESLVRGFYEVVDARLVSVFDARELELVIAGTAEIDLNDWRNN TEYRGGYHDNHIVIRWFWAAVERFNNEQRLRLLQFVTGTSSIPYEGFASLRGSNGPRRFCVEKWGKITAL PRAHTCFNRLDLPPYPSFSMLYEKLLTAVEETSTFGLE TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001001883 |
| ORF Size | 4734 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001001883.3, NP_001001883.1 |
| RefSeq Size | 11179 bp |
| RefSeq ORF | 4737 bp |
| Locus ID | 329152 |
| UniProt ID | Q6I6G8 |
| Gene Summary | E3 ubiquitin-protein ligase that mediates ubiquitination of TP73. Acts to stabilize TP73 and enhance activation of transcription by TP73. Involved in the regulation of mitotic metaphase/anaphase transition.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC224704 | Hecw2 (untagged) - Mouse HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (Hecw2), transcript variant 1, (10ug) |
CNY 20,240.00 |
|
| MR217597 | Hecw2 (Myc-DDK-tagged) - Mouse HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (Hecw2), transcript variant 1 |
CNY 15,770.00 |
|
| MR217597L3 | Lenti ORF clone of Hecw2 (Myc-DDK-tagged) - Mouse HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (Hecw2), transcript variant 1 |
CNY 17,670.00 |
|
| MR217597L4 | Lenti ORF clone of Hecw2 (mGFP-tagged) - Mouse HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (Hecw2), transcript variant 1 |
CNY 17,670.00 |
