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Pfas (NM_001159519) Mouse Tagged ORF Clone
CAT＃: MG217770
- TrueORF®
Pfas (tGFP-tagged) - Mouse phosphoribosylformylglycinamidine synthase (FGAR amidotransferase) (Pfas), (10ug)
ORF Plasmid: DDK
"NM_001159519" in other vectors (4)
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CNY 12,920.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 4432409B16Rik; FGAMS; FGAR-AT; FGARAT; Gm18; PURL; Sofa |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG217770 representing NM_001159519
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCCTGTCCTTCACTTTTATGTCCGCCCCTCTGGCCATGAGGGGGCAGCATCTGGACGTGTTTTTC GGAGACTACAAGAGAAACTGCCAACACTTCAGAGTGTGGAGACTGAGCTGTGCTACAATGTACACTGGGC AGCTGAGACCCTTCCATGGGCTGAGGAGATGAAGAAGTTGATGTGGCTGTTCGGCTGCCCTTTGGTGCGG GATGATGTTGCTCAGGAGCCCTGGCTTGTTCCTGGCTCCAACGATTTGTTGCTGGAGGTGGGACCCAGGC TGAATTTCTCGACTCCAGCATCGACCAACATTGTTTCTGTGTGCCAGGCAGCCGGGCTGAGGGCTGTGGA TCGGGTAGAGACCACCCGACGTTACCGACTTTCGTTTACCGACCACCCTACAGCTGAAATGGAAGCCATC TCTCTGGCCGCCTTGCACGACCGGATGACAGAGCAGCACTACCCTGACCCCATCCAGAGCTTCTCTCCTC AGAGCATTCCAGCCCCACTCAAGGGCTCCATCGACATACTAGCTGAGGGTCGGCCTGCCCTGGAGAAGGC CAACCAGGAGCTAGGTTTGGCTCTTGACTCCTGGGACCTCGATTTCTATACCAAGCGCTTTCAAGAGCTG CAGCGGAACCCCAGTACAGTGGAGGTCTTTGACTTGGCTCAGTCCAATAGTGAGCACAGCCGACATTGGT TCTTCAAGGGCCAGCTCCATGTGGATGGGAAGAAGCTAGCGCATTCCCTCTTTGAATCCATCATGAGCAC TCAGGCATCCTCCAACCCTAACAATGTCCTCAAGTTCTGTGACAACAGCAGTGCAATCCAGGGGAAGAAA GTCAAATTCCTGCGGCCGGAGGACTCTACCCGCCCAAGCTGCTTCCAGCAACAGCAGGGGCTTAGACATG TTGTCCTCACAGCAGAGACACATAACTTCCCCACAGGAGTGGCCCCCTTCAGCGGTGCAACCACAGGCAC GGGTGGCCGCATCAGAGACGTCCAGTGCACAGGCCGTGGGGCCCACGTGGTAGCTGGCACTGCTGGCTAT TGCTTTGGAAACCTGCACATCCCAGATTATAATCTGCCCTGGGAGGACCCAAGCTTCCAGTATCCTGGGA ACTTTGCCAGGCCTTTGGAGGTTGCTATTGAGGCCAGCAATGGAGCATCTGACTATGGCAACAAGTTTGG AGAGCCAGTGCTGGCTGGATTTGCCCGCTCTTTGGGTCTCCAGCTTCCAGATGGTCAGAGACGTGAGTGG ATCAAGCCCATCATGTTCAGTGGCGGCATTGGGTCCATGGAAGCCAAACATGTAGGCAAAAAGCCCCCAG AGCCAGGCATGGAGGTTGTAAAGGTTGGAGGTCCTGTTTATAGGATTGGAGTTGGGGGTGGAGCTGCTTC GTCTGTGCAGGTCCAGGGAGACAACACCAGTGACCTGGACTTTGGAGCTGTGCAACGGGGAGACCCGGAA ATGGAGCAAAAGATGAATCGTGTGATCCGGGCTTGTGTGGAGGCCCCAGGAGGAAACCCCATCTGCAGCC TCCATGACCAGGGTGCTGGTGGCAATGGCAATGTCCTAAAGGAGCTGAGTGACCCAGAAGGAGCCATCAT TTACACCAGCCGCTTCCAGCTAGGTGACCCAACCTTGAATGCACTGGAAATCTGGGGGGCTGAGTACCAG GAGTCAAATGCACTACTGCTTAGGCCCTCTGACCGGGACTTCCTGAGTCGTGCCAGTGCCCGGGAACGCT GCCCAGCTTGTTTTGTGGGCACAATCACTGGAGACAAGAGAATTGTACTAGTGGATGATCGTGAATGCCT AGTAGGAAAAACTGGTCAGGGAGATGCACCCCTGACACCTCCAACCCCTGTAGACCTGGACCTTGACTGG GTGCTGGGAAAGATGCCTCAGAAGGAGTTCTTCCTCCAGAGGAAACCCCCTGTGCTGCAGCCTTTGGCTT TGCCCCCAGAACTGAGTGTGCGACAGGCTCTGAACCGGGTCCTAAGGCTGCCTGCTGTGGCCAGCAAACG CTACCTCACCAACAAGGTGGATCGCTCTGTGGGTGGCCTTGTTGCCCAACAGCAGTGTGTCGGACCCCTG CAGACTCCTCTGGCTGATGTGGCTGTTGTAGCACTGAGCCACCAGGAGTGCATAGGGGCTGCCACAGCCC TGGGAGAGCAGCCAGTCAAGAGCCTGCTGGACCCCAAGGCTGCTGCCAGGCTTGCTGTGTCTGAGGCCCT CACCAACCTGGTGTTTGCTCTGGTCACCGACCTCCGAGATGTGAAGTGCAGTGGGAACTGGATGTGGGCA GCCAAGCTCCCAGGTGAGGGTGCAGCGCTGGCTGATGCCTGTGAGGCTATGGTGGCAGTGATGGCAGCAC TGGGTGTGGCAGTAGATGGTGGCAAAGACTCCCTCAGCATGGCTGCCCGGGTCGGCACTGAGACGGTGCA GGCTCCTGGGTCACTGGTCATTTCAGCATATGCTGTCTGTCCAGACATCACAGCCACTGTGACCCCAGAC CTCAAACATCCTGGTGGGAAAGGTCATTTGCTTTATGTGCCTTTGAGCCCCGGGCAGCACCGGCTGGGAG GAACGGCTCTAGCCCAGTGTTTCTCCCAGCTTGGGGAGCACCCTCCTGATCTAGACCTTCCCGAGAACCT TGTGCGTGCCTTTCATATCACCCAGGGGCTGCTGAAAGAATGCCGTCTTTGCTCAGGCCATGATGTCAGC GATGGGGGGCTTGTCACCTGCCTTCTAGAGATGGCCTTTGCTGGGAACTGTGGGATAGAGGTAGATGTAC CTGCCCCTGGGATCCATGCATTGCCTGTGCTGTTTGCGGAAGAGCCCGGTCTGGTGTTGGAGGTACAGGA GGCAGATGTGGCCGGAGTACGGCAGAGGTACGAGAGTGCTGGCCTACGGTGTCTGGAACTGGGCCACACA GGCGAGGCTGGACCCCAGGCCATGGCCCGAATATCAGTGAATAAGGCTGTGGTAGTAGAGGAGCCTGTTG GGGAGTTAAGAGCCCTCTGGGAGGAAACAAGTTTCCAGCTGGACCTACTGCAGGCAGAGCCACGCTGTGT GATAGAGGAGAAACAGGGCCTGAAGGAGCGGACAGGGCCCAGCTACTACCTGCCGCCCACCTTTCCTGTG GCCTCTGTGCCCTGTAAACCTGGTGGACCCGTCCCTCGAGTTGCCATCTTGCGAGAAGAGGGCAGCAATG GAGACCGAGAAATGGCTGATGCCTTTCACTTGGCAGGCTTTGAGGTGTGGGATGTGACCATGCAAGATCT CTGCTCTGGGGCCATCAGGTTGGACACATTCCGTGGTGTGGCCTTTGTGGGCGGCTTCAGCTACGCAGAC GTCCTGGGCTCTGCTAAAGGTTGGGCAGCTGCGGTGACCTTTAATCCACAGGCCAGGGAAGAGCTGGGCC GATTCCGAAGGCGGCCGGACACCTTCAGCCTGGGCGTGTGTAATGGCTGTCAGCTGCTGGCTCTTCTGGG CTGGGTGGGAAGTGATCCCAGTGAAGAGCAAGCTGAGCCAGGCCAGGACTCCCAGCCAACACAGCCGGGC CTCCTGCTCCGCCACAATCTGTCTGGGCGGTTCGAGTCCCGATGGGCCACCGTACGTGTGGAGCCGGGGC CAGCCCTGATGCTCCGAGGGATGGAAGGCTCTGTGCTGCCCGTGTGGAGTGCTCACGGGGAAGGCTACAT GGCATTTTCTTCTCCTGAACTCCAAGCCAAGATCGAGGCCAAGGGCCTGGTTCCTCTGCACTGGGCAGAC GATGATGGGAACCCCACAGAGCAGTACCCTCTGAACCCCAATGGATCTCCAGGGGGCATAGCTGGTATCT GTTCCCAAGATGGCCGACACCTGGCACTCATGCCGCACCCTGAGCGGGCTGTAAGGCTTTGGCAGTGGGC ATGGCGACCCTCTCCCTTTGATGTTCTTCCCACCTCCCCGTGGCTGCAGCTCTTCATCAATGCTAGGAAC TGGACCCAGGAGGACAGCTGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG217770 representing NM_001159519
Red=Cloning site Green=Tags(s) MAPVLHFYVRPSGHEGAASGRVFRRLQEKLPTLQSVETELCYNVHWAAETLPWAEEMKKLMWLFGCPLVR DDVAQEPWLVPGSNDLLLEVGPRLNFSTPASTNIVSVCQAAGLRAVDRVETTRRYRLSFTDHPTAEMEAI SLAALHDRMTEQHYPDPIQSFSPQSIPAPLKGSIDILAEGRPALEKANQELGLALDSWDLDFYTKRFQEL QRNPSTVEVFDLAQSNSEHSRHWFFKGQLHVDGKKLAHSLFESIMSTQASSNPNNVLKFCDNSSAIQGKK VKFLRPEDSTRPSCFQQQQGLRHVVLTAETHNFPTGVAPFSGATTGTGGRIRDVQCTGRGAHVVAGTAGY CFGNLHIPDYNLPWEDPSFQYPGNFARPLEVAIEASNGASDYGNKFGEPVLAGFARSLGLQLPDGQRREW IKPIMFSGGIGSMEAKHVGKKPPEPGMEVVKVGGPVYRIGVGGGAASSVQVQGDNTSDLDFGAVQRGDPE MEQKMNRVIRACVEAPGGNPICSLHDQGAGGNGNVLKELSDPEGAIIYTSRFQLGDPTLNALEIWGAEYQ ESNALLLRPSDRDFLSRASARERCPACFVGTITGDKRIVLVDDRECLVGKTGQGDAPLTPPTPVDLDLDW VLGKMPQKEFFLQRKPPVLQPLALPPELSVRQALNRVLRLPAVASKRYLTNKVDRSVGGLVAQQQCVGPL QTPLADVAVVALSHQECIGAATALGEQPVKSLLDPKAAARLAVSEALTNLVFALVTDLRDVKCSGNWMWA AKLPGEGAALADACEAMVAVMAALGVAVDGGKDSLSMAARVGTETVQAPGSLVISAYAVCPDITATVTPD LKHPGGKGHLLYVPLSPGQHRLGGTALAQCFSQLGEHPPDLDLPENLVRAFHITQGLLKECRLCSGHDVS DGGLVTCLLEMAFAGNCGIEVDVPAPGIHALPVLFAEEPGLVLEVQEADVAGVRQRYESAGLRCLELGHT GEAGPQAMARISVNKAVVVEEPVGELRALWEETSFQLDLLQAEPRCVIEEKQGLKERTGPSYYLPPTFPV ASVPCKPGGPVPRVAILREEGSNGDREMADAFHLAGFEVWDVTMQDLCSGAIRLDTFRGVAFVGGFSYAD VLGSAKGWAAAVTFNPQAREELGRFRRRPDTFSLGVCNGCQLLALLGWVGSDPSEEQAEPGQDSQPTQPG LLLRHNLSGRFESRWATVRVEPGPALMLRGMEGSVLPVWSAHGEGYMAFSSPELQAKIEAKGLVPLHWAD DDGNPTEQYPLNPNGSPGGIAGICSQDGRHLALMPHPERAVRLWQWAWRPSPFDVLPTSPWLQLFINARN WTQEDSC TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001159519 |
| ORF Size | 4011 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001159519.1, NP_001152991.1 |
| RefSeq Size | 6369 bp |
| RefSeq ORF | 4014 bp |
| Locus ID | 237823 |
| UniProt ID | Q5SUR0 |
| Gene Summary | Phosphoribosylformylglycinamidine synthase involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC224291 | Pfas (untagged) - Mouse phosphoribosylformylglycinamidine synthase (FGAR amidotransferase) (Pfas), (10ug) |
CNY 17,200.00 |
|
| MR217770 | Pfas (Myc-DDK-tagged) - Mouse phosphoribosylformylglycinamidine synthase (FGAR amidotransferase) (Pfas) |
CNY 9,152.00 |
|
| MR217770L3 | Lenti ORF clone of Pfas (Myc-DDK-tagged) - Mouse phosphoribosylformylglycinamidine synthase (FGAR amidotransferase) (Pfas) |
CNY 13,680.00 |
|
| MR217770L4 | Lenti ORF clone of Pfas (mGFP-tagged) - Mouse phosphoribosylformylglycinamidine synthase (FGAR amidotransferase) (Pfas) |
CNY 13,680.00 |
