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Rbm15b (NM_175402) Mouse Tagged ORF Clone
CAT＃: MG217915
- TrueORF®
Rbm15b (tGFP-tagged) - Mouse RNA binding motif protein 15B (Rbm15b), (10ug)
ORF Plasmid: DDK
"NM_175402" in other vectors (4)
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CNY 8,550.00
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Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 1810017N16Rik |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG217915 representing NM_175402
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGCGGCAGAGCGAGCGAGACTCCAGCCCGAGCGGGCGTGGCTCGTCATCGTCCGCCAAGCGGCCGC GGGAGCGCGAACGAGAGGCAGAGGCGGGCGGGCGGCGGGCAGCGCACAAGGCCTCCGGCGGTACCAAGCA CCCGGTCCCGGCGCGGGCTCGCGACAAGCCCCGCGGCAGCGGCGGAGGCGGCGGGCATCGCGACGGGCGC GCTGCCGGGGATGCGAATCACCGGGCGAGCGGCGGGCGCTCCTCGGGCGCGCCCGGAGGCGGGGGACGCA CCGGCAAGGCCTCCGGGGACCCGGGTGCTGGCGGCGCGTCGCCCCGCGCATCTCCACTCCCGCCGCCCCC GCCGCCCCCCGGGGCGGAACCCGCGGGTCCGGGCTCCACCGCGGCTCCGGAGTACAAGACGCTCCTCATC AGCAGCCTGAGCCCCGCGCTGCCGGCCGAGCACTTGGAGGACCGGCTCTTCCACCAGTTCAAGCGGTTCG GGGAGATCAGCCTGCGCCTGTCACACACCCCGGAGCTGGGCCGCGTGGCCTACGTGAACTTCCGGCACCC ACAGGACGCGCGGGAGGCTCGCCAGCACGCCTTGGCCCGGCAGCTGCTGCTCTACGATCGCCCGCTCAAG GTGGAGCCGGTGTACCTGCGCGGCGGCGGGAGCAGCCGGCGCAGTAGCAGCAGCAGCGCCGCCGCCTCCA CGCCGCCCCCCGGGCCTCCCGCGCCCGCCGACCCCCTGGGCTATCTGCCCCTGCACGGTGGCTACCAGTA CAAGCAGCGCTCGCTGTCTCCGGTAGCCGCCCCGCCCCTGCGGGAGCCCCGCGCGCGGCACGCCGCCGCA GCCTTCGCCCTGGATGCTGCTGCCGCGGCGGCCGTGGGACTGTCTCGAGAGCGAGCCCTGGACTATTACG GGCTGTACGACGACCGCGGCCGCCCGTACGGCTACCAGGCCGTGTGCGAGGAGGACCTGATGCCGGAGGA TGATCAGAGAGCCACTCGAAACCTCTTCATCGGCAACCTGGACCACAGTGTTTCTGAGGTGGAGCTTCGA CGGGCCTTCGAGAAGTACGGCATCATCGAGGAGGTGGTCATCAAGAGGCCTGCCCGCGGCCAGGGTGGTG CCTATGCCTTCCTCAAGTTTCAGAATCTGGACATGGCACACAGGGCGAAGGTGGCTATGTCTGGCCGGGT GATTGGCAGAAACCCCATAAAGATAGGCTACGGCAAGGCTAACCCCACCACCCGCCTCTGGGTGGGTGGT CTCGGACCTAACACCTCGCTGGCGGCCCTGGCCAGGGAATTCGATCGCTTCGGGAGCATTCGGACCATCG ATCACGTCAAAGGAGACAGCTTTGCCTACATCCAGTACGAGAGCCTGGACGCAGCCCAGGCTGCCTGTGC TAAAATGAGGGGCTTCCCCTTGGGTGGTCCAGATCGCAGGCTCCGGGTGGATTTTGCCAAAGCCGAGGAG ACTCGCTATCCCCAGCAGTACCAGCCCTCACCTCTCCCTGTGCACTATGAGCTGCTCACTGACGGATATA CCCGGCATCGGAACTTGGATGCTGACCTTAGGGTGCGGGATAGGACCCCTCCACACCTTCTGTATTCAGA CCGAGACCGGACCTTTTTGGAAGGGGACTGGACCAGCCTCAGTAAAAGTTCAGACCGCAGAAACAGCCTG GAGGGCTATAGCCGCTCAGTTCGCAGCCGGAGTGGTGAGCGCTGGGGGGGTGATGGGGACCGGAGCATAG CCAAGCCCTGGGAAGAGAGACGCAAGCGGAGGAGCCTCTCCAGTGACCGTGGGAGGACAACTCACTCCCC TTACGAGGAACGCAGCAGGACCAAGGGTGGGGGGCAGCAGTCTGAGCGAGGCTCAGATCGCACCCCTGAG CGTAGCCGCAAGGAGAACCACTCCAGTGAAGGGACCAAGGAGTCAGGCAGCAACTCCCTCAGCAACAGCA GACATGGTGCTGAGGAGAGGAGCCACCATCACCACCACCATGAGGCTCCAGACTCTTCCCATGGGAAAAA GACTAGAGAGAGTGAACGCAATCATCGGACCACTGAGGCAGAGCCCAAGACTCTTGAAGAGCCAAAACAC GAGACCAAAAAGCTAAAGACTCTGTCAGAATATGCCCAGACACTGCAGCTGGGTTGGAATGGGCTTCTGG TGTTGAAAAACAGCTGCTTTCCGACATCTATGCACATCCTTGAGGGGGACCAGGGAGTTATCAGTGGCCT CCTCAAAGACCATCCTTCTGGCAGCAAGCTGACCCAGCTAAAGATTGCCCAGCGCCTTCGACTGGATCAG CCCAAGCTGGACGAGGTCACCCGGCGAATCAAGCAGGGGAGCCCTAATGGCTATGCTGTGCTCCTGGCCA TCCAGTCAACCCCCAGCGGGCCTGGCGCCGAGGGCATGCCCGTGGTGGAGCCAGGCCTACAGAGGCGGCT TCTCAGGAACCTAGTCTCCTACTTGAAACAGAAGCAGGCTGCAGGGGTGATCAGCTTGCCGGTGGGTGGG TCTAAGGGCAGAGACAACACAGGCATGCTCTATGCCTTCCCGCCCTGCGACTTTTCACAGCAGTACCTCC AGTCAGCGCTGCGGACATTGGGCAAGTTAGAGGAAGAACACATGGTGATAGTTATAGTAAGGGACACCGC C ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG217915 representing NM_175402
Red=Cloning site Green=Tags(s) MKRQSERDSSPSGRGSSSSAKRPREREREAEAGGRRAAHKASGGTKHPVPARARDKPRGSGGGGGHRDGR AAGDANHRASGGRSSGAPGGGGRTGKASGDPGAGGASPRASPLPPPPPPPGAEPAGPGSTAAPEYKTLLI SSLSPALPAEHLEDRLFHQFKRFGEISLRLSHTPELGRVAYVNFRHPQDAREARQHALARQLLLYDRPLK VEPVYLRGGGSSRRSSSSSAAASTPPPGPPAPADPLGYLPLHGGYQYKQRSLSPVAAPPLREPRARHAAA AFALDAAAAAAVGLSRERALDYYGLYDDRGRPYGYQAVCEEDLMPEDDQRATRNLFIGNLDHSVSEVELR RAFEKYGIIEEVVIKRPARGQGGAYAFLKFQNLDMAHRAKVAMSGRVIGRNPIKIGYGKANPTTRLWVGG LGPNTSLAALAREFDRFGSIRTIDHVKGDSFAYIQYESLDAAQAACAKMRGFPLGGPDRRLRVDFAKAEE TRYPQQYQPSPLPVHYELLTDGYTRHRNLDADLRVRDRTPPHLLYSDRDRTFLEGDWTSLSKSSDRRNSL EGYSRSVRSRSGERWGGDGDRSIAKPWEERRKRRSLSSDRGRTTHSPYEERSRTKGGGQQSERGSDRTPE RSRKENHSSEGTKESGSNSLSNSRHGAEERSHHHHHHEAPDSSHGKKTRESERNHRTTEAEPKTLEEPKH ETKKLKTLSEYAQTLQLGWNGLLVLKNSCFPTSMHILEGDQGVISGLLKDHPSGSKLTQLKIAQRLRLDQ PKLDEVTRRIKQGSPNGYAVLLAIQSTPSGPGAEGMPVVEPGLQRRLLRNLVSYLKQKQAAGVISLPVGG SKGRDNTGMLYAFPPCDFSQQYLQSALRTLGKLEEEHMVIVIVRDTA TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_175402 |
| ORF Size | 2661 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_175402.5 |
| RefSeq Size | 3016 bp |
| RefSeq ORF | 2664 bp |
| Locus ID | 109095 |
| UniProt ID | Q6PHZ5 |
| Gene Summary | RNA-binding protein that acts as a key regulator of N6-methyladenosine (m6A) methylation of RNAs, thereby regulating different processes, such as alternative splicing of mRNAs and X chromosome inactivation mediated by Xist RNA. Associated component of the WMM complex, a complex that mediates N6-methyladenosine (m6A) methylation of RNAs, a modification that plays a role in the efficiency of mRNA splicing and RNA processing. Plays a key role in m6A methylation, possibly by binding target RNAs and recruiting the WMM complex. Involved in random X inactivation mediated by Xist RNA: acts by binding Xist RNA and recruiting the WMM complex, which mediates m6A methylation, leading to target YTHDC1 reader on Xist RNA and promoting transcription repression activity of Xist. Functions in the regulation of alternative or illicit splicing, possibly by regulating m6A methylation. Inhibits pre-mRNA splicing. Also functions as a mRNA export factor by acting as a cofactor for the nuclear export receptor NXF1.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC222435 | Rbm15b (untagged) - Mouse RNA binding motif protein 15B (Rbm15b), (10ug) |
CNY 11,500.00 |
|
| MR217915 | Rbm15b (Myc-DDK-tagged) - Mouse RNA binding motif protein 15B (Rbm15b) |
CNY 7,144.00 |
|
| MR217915L3 | Lenti ORF clone of Rbm15b (Myc-DDK-tagged) - Mouse RNA binding motif protein 15B (Rbm15b) |
CNY 9,690.00 |
|
| MR217915L4 | Lenti ORF clone of Rbm15b (mGFP-tagged) - Mouse RNA binding motif protein 15B (Rbm15b) |
CNY 9,690.00 |
