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Papd5 (NM_001164497) Mouse Tagged ORF Clone
CAT＃: MG217991
- TrueORF®
Papd5 (tGFP-tagged) - Mouse PAP associated domain containing 5 (Papd5) transcript variant 1, (10ug)
ORF Plasmid: DDK
"NM_001164497" in other vectors (4)
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CNY 7,410.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 5730445M16Rik; 5830428A09 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG217991 representing NM_001164497
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTCCGCTCGGGGGAGCGCCCACTGGGCGGCCTCGCCGTGCCCGCGGAGCAGCGGGACTTCCTGCCCC TGGAGACGACCAACAACAACAACAATCACCACCAGCCGGCGGCCTGGGCACGGCGGGCATCGGCGGGCCC CTCGGCGTCGCCGGTCCCATCGGCTCCCTCGTCCCCGCGACCGGCGGCCGCACTCCCCGCCTCCGAGTCT ACCGACCCGGCCTCGGGCAGCAGCAACAAGAGGAAACGTGACAACAAGGCCAGCACCTACGGACTCAACT ACAGCCTGCTGCAGCCCAGCGGAGGGCGCGCGGCCGGAGGCGGACGGGCGGACGGCGGCGGGGGCGTGTA CAGCGGGACCCCGTGGAAGCGACGGAACTACAACCAGGGAGTCGTGGGTCTGCATGAAGAAATCAGTGAT TTTTATGAGTACATGTCTCCCAGACCCGAGGAAGAGAAGATGCGGATGGAGGTAGTGAGCAGGATCGAGA GTGTGATTAAAGAGCTCTGGCCCAGTGCTGATGTCCAGATATTTGGAAGTTTTAAAACTGGCCTGTATTT ACCTACCAGTGACATCGATCTTGTGGTGTTTGGGAAGTGGGAGAACCTGCCTCTCTGGACCCTGGAAGAA GCCCTTCGGAAACACAAAGTCGCTGACGAGGATTCCGTGAAAGTTCTAGACAAAGCAACGGTTCCTATTA TTAAATTAACAGATTCTTTTACTGAAGTGAAAGTTGATATCAGCTTTAATGTCCAGAATGGCGTGAGAGC AGCAGACCTCATTAAAGATTTTACTAAGAAATATCCTGTATTGCCATACTTGGTTTTAGTATTGAAACAG TTCTTATTACAGAGGGACCTTAATGAAGTATTTACAGGTGGAATTGGTTCTTATAGTCTCTTTTTAATGG CAGTCAGTTTCCTTCAGTTACATCCCAGGGAAGATGCTTGCATCCCCAATACAAACTATGGTGTTCTCTT AATAGAGTTTTTTGAATTATATGGACGGCACTTCAATTACTTAAAGACTGGCATCCGGATAAAGGATGGT GGTTCCTATGTGGCCAAAGATGAAGTACAGAAAAATATGCTTGATGGCTACCGGCCGTCAATGCTTTATA TCGAAGATCCTTTACAACCAGGTAATGATGTTGGAAGGAGTTCCTATGGGGCCATGCAGGTGAAACAGGC CTTTGATTATGCCTATGTGGTATTGAGTCATGCAGTGTCACCGATTGCAAAGTACTATCCCAACAATGAA ACAGAGAGCATATTAGGTAGAATAATTAGAGTGACGGATGAAGTTGCCACGTATAGAGATTGGATATCAA AACAGTGGGGCTTGCAGAATAGGCCCGAGCCATCATGCAACGGAAATGGTGTTACCTTGATAGTAGATAC TCAGCAGTTAGATAAGTGTAATAATAATCTGTCTGAAGAAAAGGAAGCCCTTGGAAAATGTAGAAGTAAC GCCTCGGAACCTCTTAGTAAACACTCTTCAAACTCTTCATCAGGTCCAGTGTCCTCCTCCTCTGCCACGC AGTCCAGCTCTAGTGACGTCGATTCTGATGCAACGCCATGCAAAACCCCCAAACAGCTGCTCTGCCGTCC ACCCACTGTCACCCGGGTAGGCTCACAGGATGTCTCCTTGGAGGTCTCTCAGGCAGTTGGGAAAATGCAG AGCACTCAAACCACTAACACACCCAACAACGCCAACAAATCACAGCATGGATCAGCAAGGCTCTTCCGTT CTTCCAGCAAAGGCTTCCAAGGTACAGCTCAAACCAGCCATGGGGCCTTGATGACAAGCAAACAGCATCA AGGCAAATCCAATACTCAGTATTACCATGGCAAAAAGAGGAGACACAAGAGGGACGCGCCCCTCTCAGAG CTTTGTAGA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG217991 representing NM_001164497
Red=Cloning site Green=Tags(s) MFRSGERPLGGLAVPAEQRDFLPLETTNNNNNHHQPAAWARRASAGPSASPVPSAPSSPRPAAALPASES TDPASGSSNKRKRDNKASTYGLNYSLLQPSGGRAAGGGRADGGGGVYSGTPWKRRNYNQGVVGLHEEISD FYEYMSPRPEEEKMRMEVVSRIESVIKELWPSADVQIFGSFKTGLYLPTSDIDLVVFGKWENLPLWTLEE ALRKHKVADEDSVKVLDKATVPIIKLTDSFTEVKVDISFNVQNGVRAADLIKDFTKKYPVLPYLVLVLKQ FLLQRDLNEVFTGGIGSYSLFLMAVSFLQLHPREDACIPNTNYGVLLIEFFELYGRHFNYLKTGIRIKDG GSYVAKDEVQKNMLDGYRPSMLYIEDPLQPGNDVGRSSYGAMQVKQAFDYAYVVLSHAVSPIAKYYPNNE TESILGRIIRVTDEVATYRDWISKQWGLQNRPEPSCNGNGVTLIVDTQQLDKCNNNLSEEKEALGKCRSN ASEPLSKHSSNSSSGPVSSSSATQSSSSDVDSDATPCKTPKQLLCRPPTVTRVGSQDVSLEVSQAVGKMQ STQTTNTPNNANKSQHGSARLFRSSSKGFQGTAQTSHGALMTSKQHQGKSNTQYYHGKKRRHKRDAPLSE LCR TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001164497 |
| ORF Size | 2040 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001164497.1, NP_001157969.1 |
| RefSeq Size | 4642 bp |
| RefSeq ORF | 2043 bp |
| Locus ID | 214627 |
| UniProt ID | Q68ED3 |
| Gene Summary | Terminal nucleotidyltransferase that catalyzes preferentially the transfert of ATP and GTP on RNA 3' poly(A) tail creating a heterogeneous 3' poly(A) tail leading to mRNAs stabilization by protecting mRNAs from active deadenylation (By similarity). Also functions as a catalytic subunit of a TRAMP-like complex which has a poly(A) RNA polymerase activity and is involved in a post-transcriptional quality control mechanism. Polyadenylation with short oligo(A) tails is required for the degradative activity of the exosome on several of its nuclear RNA substrates. Doesn't need a cofactor for polyadenylation activity (in vitro). Plays a role in replication-dependent histone mRNA degradation, probably through terminal uridylation of mature histone mRNAs. May play a role in sister chromatid cohesion (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC220455 | Papd5 (untagged) - Mouse PAP associated domain containing 5 (Papd5), transcript variant 1, (10ug) |
CNY 8,740.00 |
|
| MR217991 | Papd5 (Myc-DDK-tagged) - Mouse PAP associated domain containing 5 (Papd5), transcript variant 1 |
CNY 6,750.00 |
|
| MR217991L3 | Lenti ORF clone of Papd5 (Myc-DDK-tagged) - Mouse PAP associated domain containing 5 (Papd5), transcript variant 1 |
CNY 8,650.00 |
|
| MR217991L4 | Lenti ORF clone of Papd5 (mGFP-tagged) - Mouse PAP associated domain containing 5 (Papd5), transcript variant 1 |
CNY 8,650.00 |
