Spc24 (NM_026282) Mouse Tagged ORF Clone
CAT#: MG218082
- TrueORF®
Spc24 (tGFP-tagged) - Mouse SPC24 NDC80 kinetochore complex component homolog (S. cerevisiae) (Spc24), (10ug)
ORF Plasmid: DDK
"NM_026282" in other vectors (4)
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CNY 2,850.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | 2410030K01Rik; AV109292; Spbc24 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG218082 representing NM_026282
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCTTTCCGCGACATGGTGGAGGTGAGCAACTGGCTACTGAGCCTGCTGGGGGCCAACCGCGCCG AGGCGCAGCAGCGGCGGCTGCTCGGGAGCTACGAGCAGATGATGGAGCGGCTGCTGGAGATGCAGGACGG CGCCTACCGGCAGCTTCGGGAGACTCTGGCTGTGGAGGAGGAAGTGGCTCAGAGCCTTCTTGAACTGAAA GAATGTACGCGCCAGGGGGACACCGAGCTGCAGCAGCTGGAGGTGGAGCTCCAGAGGACCAGCAAGGAGG ACACCTGTGTGCAGGCTAGGCTACGTCAGCTCATCACAGAGCTGCAGGAGCTCAGGGAGATGGAGGAAGA GCTCCAGCGCCAGGAGAGGGATGTAGATGAGGACAACACCGTCACCATCCCCTCTGCAGTGTATGTGGCT CATCTCTATCACCAAATTAGTAAAATACAGTGGGATTATGAATGCGAGCCAGGGATGATCAAGGGCATCC ACCACGGCCCCACAGTGGCCCAGCCCATCCACTTGGACAGTGCACAGCTCTCGCCGAAGTTCATCAGTGA CTACCTCTGGAGCCTGGTGGACACCACGTGGGAGCCAGAGCCT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG218082 representing NM_026282
Red=Cloning site Green=Tags(s) MAAFRDMVEVSNWLLSLLGANRAEAQQRRLLGSYEQMMERLLEMQDGAYRQLRETLAVEEEVAQSLLELK ECTRQGDTELQQLEVELQRTSKEDTCVQARLRQLITELQELREMEEELQRQERDVDEDNTVTIPSAVYVA HLYHQISKIQWDYECEPGMIKGIHHGPTVAQPIHLDSAQLSPKFISDYLWSLVDTTWEPEP TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_026282 |
ORF Size | 603 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_026282.6 |
RefSeq Size | 1349 bp |
RefSeq ORF | 606 bp |
Locus ID | 67629 |
UniProt ID | Q9D083 |
Gene Summary | Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore. The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC210586 | Spc24 (untagged) - Mouse SPC24, NDC80 kinetochore complex component, homolog (S. cerevisiae) (Spc24), (10ug) |
CNY 3,990.00 |
|
MR218082 | Spc24 (Myc-DDK-tagged) - Mouse SPC24, NDC80 kinetochore complex component, homolog (S. cerevisiae) (Spc24) |
CNY 2,400.00 |
|
MR218082L3 | Lenti ORF clone of Spc24 (Myc-DDK-tagged) - Mouse SPC24, NDC80 kinetochore complex component, homolog (S. cerevisiae) (Spc24) |
CNY 4,750.00 |
|
MR218082L4 | Lenti ORF clone of Spc24 (mGFP-tagged) - Mouse SPC24, NDC80 kinetochore complex component, homolog (S. cerevisiae) (Spc24) |
CNY 4,750.00 |