United States
Germany
Japan
United Kingdom
China
Tctn1 (NM_001039153) Mouse Tagged ORF Clone
CAT＃: MG218335
- TrueORF®
Tctn1 (tGFP-tagged) - Mouse tectonic family member 1 (Tctn1), (10ug)
ORF Plasmid: DDK
"NM_001039153" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 5,800.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | G730031O11Rik; Tect1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG218335 representing NM_001039153
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGTCGCGGGGTCTCCCGCCGCTCCTGCTGGTGCTCCTAAACTGCTACACCTCTTCGAGCACCCAGG TCATTGCCATCCCGGCTGCGGCCACCCCAGCAGTGACGAAGGAGGATCTCAATTCCACCAAGGCCACCCC CACCACTCTGCAGCCCTCCCTGTCGCCTAGGACCCCGGGAACTCCCAGGGCACCGGAGCGCTCTGGCCCC AGGCCTACCCCGGTCACGGACGTTGCTGCTCTGTGTGTCTGCGACCTGCTTCCGGCACAGTGTGATGTCA ACTGCTGTTGCGACCCTGACTGCAGCCCTGCAGATTTCAGCATCTTCTCTGCCTGCTCAGTCCCAGTTGT CACGGGTGACAGGCAGTTTTGTAGTCAGAAGGCAGCCTTCTACTCAATGAATTTGACAGCAGACCCACCT CACCGTGACTTTAAACTCATTGATCAGATCAATCCTTCCGTCTTCTGCATCCATATTTCAAACTACAAGC CTGCACTGTCTTTTGCTAATCCAGAAGTGCCTGATGAAAACAATTTTGACAGACTGATGCAGACATCAGG TGGCTTTACACTGAGTGCCGAATCCGCTGTTCCTTCCACAGCCGCATCAGATGGCCCACAGCCCACTAAA TACGAGTATGGAGCCCCTCTGCAGACTGCAGGAGCATCTTCAGGGTCATTTCTGAAACTGCCCTCGCCTC TCACGTCATCTCTGTGTGCAGATCAGAACCCCGCAGCGTTCCTGGTGAGCCAGGCCTTCGAATGCAGCCG AAGGGTGGACATAGAACAGTGTGAAGGAATGGAAGCCCTCAGCATGGCTCATTACAGCAGCCCTGCCATT CTGAGGGTACCTAATTCAATGACACAGGTCTCCATCAAGATCCAGTCTGTAATGTATCGGTCTCTGAATC ACACACTGACGCAGTTAGAAGGCCATGGTGTCCTGCGGCCATCTCTGGTCAGCACTGGGCAAGACAGACT CTGCAGCAACGTTGTTCTCCAGGTGAAGTACAGTCTGCTGTACACAGCCACAGGCCAGATCCACGAAGCC GGCCTCTCCCTCGTTCTGGGGACACTGAGCAGCGCTGTGAGTCTCCTGCAGCAGAAGTTTGAGATTCACT TTATTCAGCATGGCACCAAGCCTGTTCCTCTCAGCGGCAACCCTGGTTACAGGGTGGGACTCCCGCTGGC TGCTGGCTTTCAGCCTCAGAAGGGATCTGGGATTATTCAGACAACGAACAGACAAGGCCAATTTACTATT CTTCGTAGCACGAGCCAGCAGGATTGCCTTGCCAGCGAGGGGCTTCGCACCCCTGTGCTATTTGGCTACA ATGTGCAGTCTGGCTGTCAGCTCAGGTTGACAGGCACCATCCCTTGTGGGCTTCTGGCACAGAAGGTGCA GGACCTGCTGAGAGGCCAGGCCTTCCCTGACTATGTGGCTGCCTTTGGGAACTCCCGGGCCCAGGATGTG CAGGACTGGGTGCCTGTGCACTTTGTCACCTACTCCTCCAACATGAAGGGCTCTTGCCAGCTTCCGGTGG CTTTGGCTATAGAAGTGAAATGGACTAAGTATGGGTCCCTGCTGAACCCCCAGGCCAGGATAGTAAATGT CACTGCCCAGCTCGTCTCAGTTCCAGAGCCCCTCCCAGGGCCTGAGAGGACCGTGGTCATTTCCACTGCA GTGACATTTGTGGATGTGTCAGCCCCTGCAGAGGCAGGCTTCCGAGCCCCACCCACCATCAACGCCAGGC TACCCTTCAGCTTCTTCTTCCCGTTTGTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG218335 representing NM_001039153
Red=Cloning site Green=Tags(s) MGSRGLPPLLLVLLNCYTSSSTQVIAIPAAATPAVTKEDLNSTKATPTTLQPSLSPRTPGTPRAPERSGP RPTPVTDVAALCVCDLLPAQCDVNCCCDPDCSPADFSIFSACSVPVVTGDRQFCSQKAAFYSMNLTADPP HRDFKLIDQINPSVFCIHISNYKPALSFANPEVPDENNFDRLMQTSGGFTLSAESAVPSTAASDGPQPTK YEYGAPLQTAGASSGSFLKLPSPLTSSLCADQNPAAFLVSQAFECSRRVDIEQCEGMEALSMAHYSSPAI LRVPNSMTQVSIKIQSVMYRSLNHTLTQLEGHGVLRPSLVSTGQDRLCSNVVLQVKYSLLYTATGQIHEA GLSLVLGTLSSAVSLLQQKFEIHFIQHGTKPVPLSGNPGYRVGLPLAAGFQPQKGSGIIQTTNRQGQFTI LRSTSQQDCLASEGLRTPVLFGYNVQSGCQLRLTGTIPCGLLAQKVQDLLRGQAFPDYVAAFGNSRAQDV QDWVPVHFVTYSSNMKGSCQLPVALAIEVKWTKYGSLLNPQARIVNVTAQLVSVPEPLPGPERTVVISTA VTFVDVSAPAEAGFRAPPTINARLPFSFFFPFV TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001039153 |
| ORF Size | 1779 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001039153.3, NP_001034242.2 |
| RefSeq Size | 2068 bp |
| RefSeq ORF | 1782 bp |
| Locus ID | 654470 |
| UniProt ID | Q8BZ64 |
| Gene Summary | Component of the tectonic-like complex, a complex localized at the transition zone of primary cilia and acting as a barrier that prevents diffusion of transmembrane proteins between the cilia and plasma membranes. Regulator of Hedgehog (Hh), required for both activation and inhibition of the Hh pathway in the patterning of the neural tube. During neural tube development, it is required for formation of the most ventral cell types and for full Hh pathway activation. Functions in Hh signal transduction to fully activate the pathway in the presence of high Hh levels and to repress the pathway in the absence of Hh signals. Modulates Hh signal transduction downstream of SMO and RAB23.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC219476 | Tctn1 (untagged) - Mouse tectonic family member 1 (Tctn1), (10ug) |
CNY 7,510.00 |
|
| MR218335 | Tctn1 (Myc-DDK-tagged) - Mouse tectonic family member 1 (Tctn1) |
CNY 4,416.00 |
|
| MR218335L3 | Lenti ORF clone of Tctn1 (Myc-DDK-tagged) - Mouse tectonic family member 1 (Tctn1) |
CNY 7,130.00 |
|
| MR218335L4 | Lenti ORF clone of Tctn1 (mGFP-tagged) - Mouse tectonic family member 1 (Tctn1) |
CNY 7,130.00 |
