Aanat (NM_009591) Mouse Tagged ORF Clone
CAT#: MG218438
- TrueORF®
Aanat (tGFP-tagged) - Mouse arylalkylamine N-acetyltransferase (Aanat) transcript variant 1, (10ug)
ORF Plasmid: DDK
"NM_009591" in other vectors (4)
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CNY 2,850.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | AA-NAT; Na; Nat; Nat-2; Nat4; S; Snat |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG218438 representing NM_009591
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTGAACATCAACTCCCTGAAACCTGAGGCCCTGCACCTGCCACTTGGGACCTCAGAGTTCCTAGGCT GCCAGCGGCGCCACACACTCCCTGCCAGTGAGTTCCGCTGCCTCACACCTGAGGATGCCACCAGTGCGTT TGAGATTGAGCGGGAAGCCTTTATCTCTGTCTCCGGTACCTGTCCCCTCTACTTGGATGAGATCCGGCAC TTCCTAACCCTGTGTCCAGAGCTGTCACTGGGCTGGTTTGAGGAAGGCTGCCTTGTGGCCTTCATCATTG GCTCGCTGTGGGACAAGGAGAGACTTACTCAGGAGTCGCTGACACTACACAGGCCCGGAGGCCGCACAGC CCACCTGCACGTACTGGCGGTGCACAGAACCTTCCGGCAGCAGGGCAAGGGCTCTGTCCTCCTGTGGAGA TACCTTCACCACCTGGGCAGTCAGCCGGCCGTGCGCCGGGCTGTGCTCATGTGTGAGGATGCCCTGGTAC CCTTCTATGAGAAATTTGGCTTCCAGGCTGTGGGCCCATGTGCCATCACCGTGGGCTCTCTCACCTTCAC GGAGCTACAGTGTTCCTTACGATGCCACGCCTTCCTGCGCAGGAACAGCGGCTGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG218438 representing NM_009591
Red=Cloning site Green=Tags(s) MLNINSLKPEALHLPLGTSEFLGCQRRHTLPASEFRCLTPEDATSAFEIEREAFISVSGTCPLYLDEIRH FLTLCPELSLGWFEEGCLVAFIIGSLWDKERLTQESLTLHRPGGRTAHLHVLAVHRTFRQQGKGSVLLWR YLHHLGSQPAVRRAVLMCEDALVPFYEKFGFQAVGPCAITVGSLTFTELQCSLRCHAFLRRNSGC TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_009591 |
ORF Size | 615 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_009591.3, NP_033721.1 |
RefSeq Size | 1370 bp |
RefSeq ORF | 618 bp |
Locus ID | 11298 |
UniProt ID | O88816 |
Gene Summary | The protein encoded by this gene belongs to the acetyltransferase superfamily. It is the penultimate enzyme in melatonin synthesis and controls the night/day rhythm in melatonin production in the vertebrate pineal gland. Melatonin is essential for the function of the circadian clock that influences activity and sleep. This enzyme is regulated by cAMP-dependent phosphorylation that promotes its interaction with 14-3-3 proteins and thus protects the enzyme against proteasomal degradation. This gene may contribute to numerous genetic diseases such as delayed sleep phase syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2010] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC208096 | Aanat (untagged) - Mouse arylalkylamine N-acetyltransferase (Aanat), transcript variant 1, (10ug) |
CNY 3,990.00 |
|
MR218438 | Aanat (Myc-DDK-tagged) - Mouse arylalkylamine N-acetyltransferase (Aanat), transcript variant 1 |
CNY 2,400.00 |
|
MR218438L3 | Lenti ORF clone of Aanat (Myc-DDK-tagged) - Mouse arylalkylamine N-acetyltransferase (Aanat), transcript variant 1 |
CNY 4,750.00 |
|
MR218438L4 | Lenti ORF clone of Aanat (mGFP-tagged) - Mouse arylalkylamine N-acetyltransferase (Aanat), transcript variant 1 |
CNY 4,750.00 |