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Rft1 (NM_177815) Mouse Tagged ORF Clone
CAT＃: MG218503
- TrueORF®
Rft1 (tGFP-tagged) - Mouse RFT1 homolog (S. cerevisiae) (Rft1), (10ug)
ORF Plasmid: DDK
"NM_177815" in other vectors (4)
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CNY 5,040.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | D930025H04 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG218503 representing NM_177815
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCAGCCAGGAGGTGCTAGGCCAGGCGGCCCGGTTGGCCTCCTCGGGTCTCCTGCTGCAGGTCTTGT TTCGATTAATCACCTTTGTCTTGAATGCATTTATTCTTCGATTCCTGTCAAAGGAGATTGTGGGCATAGT GAATGTCAGACTAACGCTGCTTTACTCAACCACAACCTTCCTGGCCAGGGAGGCCTTCCGAAGAGCTTGT CTGAGCGGGGGTGCCCAGCGGGACTGGAGTCAGACCCTCAACCTCCTGTGGCTAACTGTCCCACTGGGTA TATTTTGGTCCTCCTGCTTGGGCTGGGTCTGGTTGCAGTTGCTTGAAGTGCCTGATCCAGATGTGGTCCC CTACTATGGAACTGGTGTGCTGTTCTTTGGTCTCTCAGCAGTTGTGGAACTTCTGGGAGAGCCTTTCTGG GTCTTGGCACAAGCACATATGTTTGTTAAGCTCAAGGTTCTTGCTGAGAGCATGTCGGTCATCCTTAGGA GTGTCCTGACGGCTCTTCTTGTCCTGTGGCTGCCTCACTGGGGTCTGTACATCTTTTCTTTGGCTCAGCT TCTCTACACGACTGTTCTGGTGCTCTGCTATGCCATCTATTTAATACAATTACTGCGTTCCCCAGAATCA GCCAAGCAACTAACTCTTCCTGTCTCCAGAGTAACACAACTGTTGCCCAGTATTTCAAGAAGCAGAGCAT TTGTCAACTGGAAGGAGGCAGGATTGGCTTGGAGCTTCTTCAAACAGTCGTTCTTGAAGCAGATTTTGAC AGAAGGCGAGCGGTACGTGATGACATTTTTGAATGTGTTAAACTTTGGCGATCAGGGTGTATATGACATA GTGAATAATCTTGGCTCCCTTGTGGCCAGGCTAATTTTCCAGCCAGTAGAGGAGAGTTTTTATTTATTCT TTGCTAAAGTGCTAGAGAGAGAGAAGGACGCCTCACTTCAGAAGCAGGACGATGTTGCTGTGGCTGCTGC GGTTTTGGAGTCCCTCCTCAAGCTGGCCTTGCTGACTGGACTGACCATGACTGTTTTTGGCTTTGCCTAT TCCCAACTGGCTTTGGACATCTATGGAGGAGCCATGCTTAGTTCGGGATCTGGCCCTGTGCTGATGCGTT GCTATTGCCTCTATGTCCTCCTGCTTGCTATCAATGGTGTGACCGAGTGTTTCATGTTTGCCGCCATGAG CAAAGAGGAGGTTGACAGGTACAATTTCACCATGCTGGCACTGTCATCCTCGTTTTTAGTCTTATCCTAC CTGCTGACCAGTTGGTGTGGCAGTGTGGGCTTCATCATGGCCAACTGCTTTAACATGGGCATCCGGATCA CACAGAGTCTTTCCTTCATCCATCACTACTTCCGAGAGAGCCCTCACAGACCCCTGGCTGGCCTGCGCCT GTCACCAGTTCTTCTTGGGGTGTTCATTCTCAGTGCTGGGATTACTAGTGTCTCAGAGGCATTCCTCTGC TGTGAGAGGGGCTGGCCAGCTAGGCTGGCACACATCGCTGTGGGGACCATCTGCTTAGGAGTGACTCTTG GGACAGCGTTCCTCACAGAGACCAAGCTGATCCACTTTCTCAGGACTCAGTTGGGTAGATCCAGACTCAG TGACAAAATGACA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG218503 representing NM_177815
Red=Cloning site Green=Tags(s) MGSQEVLGQAARLASSGLLLQVLFRLITFVLNAFILRFLSKEIVGIVNVRLTLLYSTTTFLAREAFRRAC LSGGAQRDWSQTLNLLWLTVPLGIFWSSCLGWVWLQLLEVPDPDVVPYYGTGVLFFGLSAVVELLGEPFW VLAQAHMFVKLKVLAESMSVILRSVLTALLVLWLPHWGLYIFSLAQLLYTTVLVLCYAIYLIQLLRSPES AKQLTLPVSRVTQLLPSISRSRAFVNWKEAGLAWSFFKQSFLKQILTEGERYVMTFLNVLNFGDQGVYDI VNNLGSLVARLIFQPVEESFYLFFAKVLEREKDASLQKQDDVAVAAAVLESLLKLALLTGLTMTVFGFAY SQLALDIYGGAMLSSGSGPVLMRCYCLYVLLLAINGVTECFMFAAMSKEEVDRYNFTMLALSSSFLVLSY LLTSWCGSVGFIMANCFNMGIRITQSLSFIHHYFRESPHRPLAGLRLSPVLLGVFILSAGITSVSEAFLC CERGWPARLAHIAVGTICLGVTLGTAFLTETKLIHFLRTQLGRSRLSDKMT TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_177815 |
| ORF Size | 1623 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_177815.3, NP_808483.2 |
| RefSeq Size | 2384 bp |
| RefSeq ORF | 1626 bp |
| Locus ID | 328370 |
| UniProt ID | Q8C3B8 |
| Gene Summary | May be involved in N-linked oligosaccharide assembly. May participate in the translocation of oligosaccharide from the cytoplasmic side to the lumenal side of the endoplasmic reticulum membrane (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC218689 | Rft1 (untagged) - Mouse RFT1 homolog (S. cerevisiae) (Rft1), (10ug) |
CNY 6,940.00 |
|
| MR218503 | Rft1 (Myc-DDK-tagged) - Mouse RFT1 homolog (S. cerevisiae) (Rft1) |
CNY 4,032.00 |
|
| MR218503L3 | Lenti ORF clone of Rft1 (Myc-DDK-tagged) - Mouse RFT1 homolog (S. cerevisiae) (Rft1) |
CNY 6,460.00 |
|
| MR218503L4 | Lenti ORF clone of Rft1 (mGFP-tagged) - Mouse RFT1 homolog (S. cerevisiae) (Rft1) |
CNY 6,460.00 |
