Brwd1 (NM_001103179) Mouse Tagged ORF Clone
CAT#: MG218689
- TrueORF®
Brwd1 (tGFP-tagged) - Mouse bromodomain and WD repeat domain containing 1 (Brwd1) transcript variant 2, (10ug)
ORF Plasmid: DDK
"NM_001103179" in other vectors (2)
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CNY 24,800.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | 5330419I02Rik; D530019K20Rik; G1-403-16; repro5; Wdr9 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG218689 representing NM_001103179
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGAGCCTTCGCCGGCCCGACGTCCGGTGCCTCTCATCGAGTCGGAGCTGTACTTTCTCATCGCCC GCTACCTCTCAGCTGGCCCGTGTCGCCGAGCGGCGCAGGTGCTGGTGCAGGAGCTGGAGCAGTACCAGCT GTTGCCGAAGAGGTTGGACTGGGAGGGCAACGAGCACAACAGGAGCTACGAGGAATTGGTCTTGTCCAAT AAACATGTGGCTCCTGACCACCTACTACAGATATGCCAGCGCATCGGTCCGATGCTGGATAAGGAGGTCC CGCCCAGTATCTCAAGAGTCACTTCTTTGCTTGGTGCAGGAAGGCAGTCTTTGCTACGAACAGCAAAAGA CTGCAGGCACACAGTGTGGAAGGGCTCTGCCTTTGCTGCTCTTCACAGAGGAAGACCTCCTGAAATGCCC GTCAATTATGGCCCCCCACCGAGTCTTGTGGAGATACACCGAGGAAGGCAGCTCACAGGGTGTTCCACTT TTAGCACAGCGTTCCCAGGAACTATGTACCAGCATATAAAGATGCACAGGAGGATTCTTGGACACCTGTC TGCAGTGTACTGTGTAGCATTTGATAGGACAGGACATAGAATCTTTACAGGTTCAGATGATTGCTTGGTA AAGATTTGGTCAACACATAATGGCCGCTTGTTGTCTACGTTAAGAGGCCATTCTGCAGAGATTTCAGACA TGGCTGTGAACTACGAGAACACGCTGATTGCTGCAGGAAGCTGTGATAAGATCATTAGGGTGTGGTGCTT GAGGACCTGCGCCCCAGTTGCTGTGCTGCAAGGACACACGGGGTCAATCACATCTCTGCAGTTTAGCCCG ATGGCCAAAGGACCTCAGCGGTACATGGTTTCTACGGGCGCTGATGGCACTGTTTGCTTCTGGCAGTGGG ATTTAGAGTCCTTGAAGTTCAGTCCACGACCCCTGAAGTTCACGGAAAAGCCAAGGCCTGGTGTTCAGAT GCTGTGTTCTTCATTTAGCGTTGGTGGTATGTTTTTAGCTACAGGTAGTACTGATCATGTAATCAGAATG TATTTTTTGGGATTCGACGCACCTGAAAAAATCGCCGAGCTTGAAAGCCACACTGACAAAGTTGATAGTA TCCAGTTTTGTAACAATGGTGACCGGTTCTTAAGTGGTAGCCGAGATGGAACAGCACGGATCTGGAGGTT TGAGCAGTTGGAATGGAGAAGCATTTTACTGGACATGTCTGCCAGGATCTCGGGAGACACATCTTCAGAA GAAGAGAGGTTTATGAAACCCAAAGTTACAATGATTGCTTGGAACCAGGATGATAGCACCGTGGTCACAG CCGTGAATGATCATGTCCTCAAGGTGTGGAGTTCCTACACTGGACAGCTGCTTCATAACTTACTGGGACA TGCTGATGAAGTGTTTGTTTTGGAGACACATCCCTTTGATTCCAGAATTATGTTATCTGCAGGACACGAT GGCAGCATATTTATATGGGATATTACAAAGGGCATCAAGATGAAGCATTACTTTAATATGATCGAAGGAC AAGGACATGGAGCTGTGTTTGACTGCAAGTTTTCACAGGATGGACAACATTTTGCCTGTACAGATTCTCA CGGGCATCTTCTGATATTTGGCTTTGGATGCAGCAAGCCATATGAAAAGATTCCTGATCAGATGTTTTTT CATACTGACTACCGACCACTGATAAGAGATTCCAATAACTATGTCTTGGATGAGCAGACACAGCAGGCAC CTCATCTTATGCCTCCACCATTCTTGGTGGATGTGGACGGAAACCCTCATCCAACCAAGTTCCAGAGATT AGTACCAGGTCGAGAAAACTCAGCAGATGAACATTTGGTTCCACAGCTAGGCTATGTGGCCACAAGTGAT GGGGAGGTGATTGAACAGATTATAAGCCTGCATACCAACGACAGTGGAGATGCAAGCCCAGAGTCCAGCG TCTTGGATGGCATGATTAGGCAGCTGCAGCAGCAGCAAGACCAGAGACTGGGAGTGGATCAGGATGGGAC TGCAAATGGACTTCCCAGTGGGGAAGGGACACCCCGAAGAGGGAGTTTTAGAAGACTGAGTTTAGACATT CAGTCTCCTCCAAACATCGGTCTCCGTCGGAGTGGGCAGGTTGAAGGTGTTCGTCAGATGCATCAGAACG CTCCTCGGAGTCAGATTGCTACAGAGCGTGACCTCCAGGCTTGGAAGCGGAGAGTAGTTGTTCCAGAGGC ACCGCCTGGCATGTTCAGGCGGTTGGAAGACATTCGCATAGAGAGAGGCGAGGAGGAGAGGAACCTGTAT GTAATAGGGAGAAAGAAGAAGACTTTCCAGGTCCCACAAAAGTCCAATTCGATGGTTTTGGTGTCCCAGT CTAGACAACGGACGTGTAGGCGTAAATATGCAAATTATAGTAGAAGAAACGCTGACCAGTGTGAATTATC TTCTGGAAATGAATCTTCAGGCTCTGTTAGACATGAGACATCTTATGATCAGAGTGAAGGTTCTTGTTCT TCAGAAGATGACGAGTGGAGAAATGACAGAAGAAGTGGTAGTGACAGCGACAGCTCAAGTGACTCTTCAT CTCAGTATTCTGATTGGACAGCTGACACGGGGATCAACTTGCAGCCTCCTCTCAGGATGTCATGTCGTCG TCGAGTAACTCGATTCTGCAGTACTTCAGAGGACGAAATGTCCATGGAGAACATATCACCTCCTAAACGA AGGCGGAAAAGAAGGAAAGAAAGCAAGCCTAAGAGGGAGAGTTTACGAAGGATGACTCCAGCAGAACTTG CAAATATGGAACACCTATATGAGTTTCACCCTCCTGTGTGGATAACGGACACCACACTTCGGAAGTCTCC CTTTGTTCCTCAGATGGGTGATGAGGTGATGTACTTCCGTCAGGGTCATGAAGCTTATATTGAAGCTGTC AGAAGAAATAACATTTATGAACTAAATCCTCACAAGGAGCCATGGAGGAAAATGGATCTTAGGGATCAAG AACTGGTCAAAATTGTTGGACTAAGGTATGAGGTTGGACCACCTACACTTTGTTGCCTCAAACTAGCATT TATAGATCCAGCGACTGGCAGACTGACGGATAAATCTTTCTCTATTAGATATCATGACATGCCAGATGTT ATTGATTTTCTTGTGTTGCGCCAATTTTATGATGAAGCAAGACAGAGAAACTGGCAGCCTTGTGACAGAT TTCGATCTATAATTGATGATGCTTGGTGGTTTGGAACCGTGTTGAGTCAAGAACCGTATCAACCCCAGTA TCCTGACAGTCACTTCCAGTGTTACATTGTTAGGTGGGACAACACCGAGACTGAAAAGCTTAGCCCTTGG GACATGGAACCGATTCCTGACAATGTTGATCCTCCAGAAGAATTGGGAGCAAGTATCTCTGTCACTTCAG ATGAGCTAGAGAAATTGCTCTACAAACCACAAGAAGGAGAATGGGGCCAGAGGTCAAGAGATGAGGAGTG TGATCGGATCATCAGTGGCATAGACCAGCTTTTAAACCTCGATATAGCAGCAGCGTTTGCAGGCCCGGTG GATCTGTGCACATATCCCAAGTACTGCACAGTAGTGGCTTATCCAACTGATCTCTACACAATTCGAATGA GGCTTGTTAATCGATTTTACAGGCGGTTATCTGCATTAATTTGGGAAGTCCGATATATAGAGCATAATGC AAGAACGTTTAATGAACCGGAAAGTGTAATTGCAAGATCAGCTAAAAAAATAACTGACCAGCTTCTGAAG TTTATCAAGAATCAAGATTGTACAAATATATCGGAACTATGTAACACTTCTGACAATGATGAGGACGACA GTGCTGATGATTTGGATGATAGTGATCTTCCTAAAACGTCTTCTGGAAGAAGGAAGGTCCACAACTGGAA AAGGAGGAGTAGAGCCAGTGGCTGTATTGAGAGTGACTGGAGGAGGCAGTGCAAGGCCCTGCTTATCCTC ATCTTCCAGTGTGAGGACTCGGAGCCCTTTAGACAGCCCGTGGACTTGGATGAGTACCCAGATTACCGAG ATATTATAGATACGCCAATGGATTTTGGAACAGTAAGGGAAACTCTAGAAGCTGGGAATTACGATAGCCC TGTGGAGTTTTGCAAAGACATTCGATTGATATTTAGCAATGCAAAGGCATATACACCGAACAAAAGATCA AAGATTTATAGTATGACCTTGAGATTGTCTGCTTTGTTTGAAGAAAAAATGAAGAAAATCTCCTTTGATT TTAAAATTGGTCAAAAATTCAAGGAGAAACTTCGTAGAAGCCAAAGATTCAAGCAACGACAAAACTGTAA CGGTGCTGTCCCTGGTGACAGGCGCAGGAGAAATGTCAAGCAGAAGCAGTTCAAATCTCAGACAAAAGTA ATTCCACAGTTGATGTGTCCTCCTTCTCAGTCTACCTCAAGTAAGGTACCTTTGTCTGCAACCCGCAAGA CAAGTGCTGGTGTCTCTTCAGGCTTTACTTCTGGGGACTCTTCAGACTCTGCAGGATCTTTAGAAAGAGT CAGAAGACAGAGGCCTGAAGTACTCAGGAGTGGTTCTGTGCTGTTTGGAAGTGAAATGGAAGATTTCTTA GCTACCTCTTCATCTTCTTCAGCTTCAAACAGTTCAGAGGAAAGCAAAGCGAGTCCTGGAGCTCGAGAAT CTTCCTTGCGCAGTGGTGTGTTGAGAGGCAGCAACCTCGGGGTGACCAGAACAAGAGCTGCTCGGAGGAA AGCTGGTTCTGTTTCTTTAGAAAATGGGTGTGGTAGAAAAGCCACTCGGAAGAGAGTCTATTTAAGTGAT TCTGATAACAATTCAGTGGAAACAGATGAAAATCTGAAAAATAGAAAATGTGGAAGTAGCCGGAAAGTTC TGCGGAAGTGTGCTGCTGTTGCTGCCAGCAAGATAAAGCTGATGAGTGATGCAGAGGATTCGAGCTCAGA GAGCCCCTGCTCTGGGCGGAAGCTGCCACATCGAAACGCTTCAGCTGTGGCCAGGAAGAAGTTGCTACAC AACTCTGATGATCAGAGCCTTAAGTCAGAGACAGAAGAACTGAAAGACCAGAACCAATCACTGTTGATAT CCGGTCCCCATTCTGTCCACAACAGCATCAGTGACTCAGAGTCAGACAGTGACTTGCGAGCAACCCGGAA GACCTGGAATGCTAACGGCTGCACATCCCACACTGCAGCAACATGCAAAACCAAGTCACGTCCAATAGAG TCTTCTGAGGAAGATTCCAGATGTCATGGATCTGATCATGGGCCCAGCAGCACTGGGGACCCTTCAACAT CTGGGCAGAAACTCAGGGCAGATAGCATCTCTGAGGAAGCTGACTCTGAACCAGAAAGCAGTGTACTATG CAAGAATACACATCTGTGTAAGAAAGCCAAGATCTTGAGTGACTCAGAAGACTGTGAGGAAAAGTGTGGA GAAAGGAGAGGTCCTGAAGTGGAAGGAAGCCCAGTTTCTGAAGCCTTGAGGGAAGCCATTCTTGCCCCCC AGTGTCTCTCACATCGTGGATCTGAGACTGATGTGGATTCTGACGGCGGTGCAGTGAGAGAGAAATCATA CAGCAATGAAAACGGATCTGTATCACTAGAGAATGGACAAAGAAGAAAAGTTTCCCGAAAAAGTTCCAGT GACAAGGAAAGTAATCTACAGGTAACCCAGAAGTCACCCAAAGACAGAAGCAGCCCCTCGAGGATTACTC AGCGTGCTAGTGTGGCTACTGATAAAATGAAGCTCACAAGTGATGCAGAAGACCTCAGCCTAGAGAGCGT GTGTACTCGAAGCAAAAGGAGAAGGAAAAAGCCTGCTCGATTTGCTTGTACCCCAGCAAAGACTGCATTG AGTAGTGAGGAGAAACACGCACATTGTGAAGTGCCTGAGGCACAGCCTGCCTGCAGGAACAAGCTCCCTG AGCCTGAACACCAAGATAGTGCAGAGAATCCCAGTCAGGCTGCAAGTGCAGACTTGAATTCTGGAGGGGG TTCCAGTTTTGAACAAAGAAAAAGCATACAGAGCAGGCAAATGGGCGCTGTCTGCGTAAGGCCACCTCCT AAAACCCAAAGCTCCTCTGCTGGGCTTTCTCAGGAAAACGCCAGAAGCCAAACCCTAGATAGTGAGACTA GCTTGCCTTCTGAGTCGGTTTTGACCCAGAAAGCTACTGTAGAGAGTAACTTTGAAGAAGAACTGAACTA TGGATTAAGAAGGTGGAATGGCAGAAGGCTCAGGACATACGGGAAGGCCCCACTCAGTAGGACAGCACAA GTTACTCCCAGTTTACAGGCCTCAGCAGAGGTCGGGGTGAAGAGGAGGAGAATGCATCCTGAGGTAGATG GTGAGGACGTGCCCGGACAAATGGGGAGTTCAGGATGCGGACCTGACACCAGTCCCAAGGCATCAGACTT GGGGTCAGTAACTGACTCTGATGTTGACTGTACCGATAACACACAAACCCAAAGGAAGAAGAAAAGGAAA GGGAAAGCAAGAGTGCTTAGCAAAGGTAAACCCTCCTCATCCAACTCACCTAAAACTGCAAGACATCCAA GGCAGAGTAAAGGTCCTAGGTTAAATGTGGATGACAATGACTGGGAGGATTTGGACTATGCAAAAGCTAA AGGAGTTGTCCGGCTCTCAAAAATCCAAACGAGAAATCAGGGTAGGAGAACGGTGAGATACCACGATGGG GAAGATGATAGAAGCATAGAGAGCGCACTAGAGCTCACTGACCGCACCTTACGCCCT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG218689 representing NM_001103179
Red=Cloning site Green=Tags(s) MAEPSPARRPVPLIESELYFLIARYLSAGPCRRAAQVLVQELEQYQLLPKRLDWEGNEHNRSYEELVLSN KHVAPDHLLQICQRIGPMLDKEVPPSISRVTSLLGAGRQSLLRTAKDCRHTVWKGSAFAALHRGRPPEMP VNYGPPPSLVEIHRGRQLTGCSTFSTAFPGTMYQHIKMHRRILGHLSAVYCVAFDRTGHRIFTGSDDCLV KIWSTHNGRLLSTLRGHSAEISDMAVNYENTLIAAGSCDKIIRVWCLRTCAPVAVLQGHTGSITSLQFSP MAKGPQRYMVSTGADGTVCFWQWDLESLKFSPRPLKFTEKPRPGVQMLCSSFSVGGMFLATGSTDHVIRM YFLGFDAPEKIAELESHTDKVDSIQFCNNGDRFLSGSRDGTARIWRFEQLEWRSILLDMSARISGDTSSE EERFMKPKVTMIAWNQDDSTVVTAVNDHVLKVWSSYTGQLLHNLLGHADEVFVLETHPFDSRIMLSAGHD GSIFIWDITKGIKMKHYFNMIEGQGHGAVFDCKFSQDGQHFACTDSHGHLLIFGFGCSKPYEKIPDQMFF HTDYRPLIRDSNNYVLDEQTQQAPHLMPPPFLVDVDGNPHPTKFQRLVPGRENSADEHLVPQLGYVATSD GEVIEQIISLHTNDSGDASPESSVLDGMIRQLQQQQDQRLGVDQDGTANGLPSGEGTPRRGSFRRLSLDI QSPPNIGLRRSGQVEGVRQMHQNAPRSQIATERDLQAWKRRVVVPEAPPGMFRRLEDIRIERGEEERNLY VIGRKKKTFQVPQKSNSMVLVSQSRQRTCRRKYANYSRRNADQCELSSGNESSGSVRHETSYDQSEGSCS SEDDEWRNDRRSGSDSDSSSDSSSQYSDWTADTGINLQPPLRMSCRRRVTRFCSTSEDEMSMENISPPKR RRKRRKESKPKRESLRRMTPAELANMEHLYEFHPPVWITDTTLRKSPFVPQMGDEVMYFRQGHEAYIEAV RRNNIYELNPHKEPWRKMDLRDQELVKIVGLRYEVGPPTLCCLKLAFIDPATGRLTDKSFSIRYHDMPDV IDFLVLRQFYDEARQRNWQPCDRFRSIIDDAWWFGTVLSQEPYQPQYPDSHFQCYIVRWDNTETEKLSPW DMEPIPDNVDPPEELGASISVTSDELEKLLYKPQEGEWGQRSRDEECDRIISGIDQLLNLDIAAAFAGPV DLCTYPKYCTVVAYPTDLYTIRMRLVNRFYRRLSALIWEVRYIEHNARTFNEPESVIARSAKKITDQLLK FIKNQDCTNISELCNTSDNDEDDSADDLDDSDLPKTSSGRRKVHNWKRRSRASGCIESDWRRQCKALLIL IFQCEDSEPFRQPVDLDEYPDYRDIIDTPMDFGTVRETLEAGNYDSPVEFCKDIRLIFSNAKAYTPNKRS KIYSMTLRLSALFEEKMKKISFDFKIGQKFKEKLRRSQRFKQRQNCNGAVPGDRRRRNVKQKQFKSQTKV IPQLMCPPSQSTSSKVPLSATRKTSAGVSSGFTSGDSSDSAGSLERVRRQRPEVLRSGSVLFGSEMEDFL ATSSSSSASNSSEESKASPGARESSLRSGVLRGSNLGVTRTRAARRKAGSVSLENGCGRKATRKRVYLSD SDNNSVETDENLKNRKCGSSRKVLRKCAAVAASKIKLMSDAEDSSSESPCSGRKLPHRNASAVARKKLLH NSDDQSLKSETEELKDQNQSLLISGPHSVHNSISDSESDSDLRATRKTWNANGCTSHTAATCKTKSRPIE SSEEDSRCHGSDHGPSSTGDPSTSGQKLRADSISEEADSEPESSVLCKNTHLCKKAKILSDSEDCEEKCG ERRGPEVEGSPVSEALREAILAPQCLSHRGSETDVDSDGGAVREKSYSNENGSVSLENGQRRKVSRKSSS DKESNLQVTQKSPKDRSSPSRITQRASVATDKMKLTSDAEDLSLESVCTRSKRRRKKPARFACTPAKTAL SSEEKHAHCEVPEAQPACRNKLPEPEHQDSAENPSQAASADLNSGGGSSFEQRKSIQSRQMGAVCVRPPP KTQSSSAGLSQENARSQTLDSETSLPSESVLTQKATVESNFEEELNYGLRRWNGRRLRTYGKAPLSRTAQ VTPSLQASAEVGVKRRRMHPEVDGEDVPGQMGSSGCGPDTSPKASDLGSVTDSDVDCTDNTQTQRKKKRK GKARVLSKGKPSSSNSPKTARHPRQSKGPRLNVDDNDWEDLDYAKAKGVVRLSKIQTRNQGRRTVRYHDG EDDRSIESALELTDRTLRP TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001103179 |
ORF Size | 6777 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001103179.1, NP_001096649.1 |
RefSeq Size | 7547 bp |
RefSeq ORF | 6780 bp |
Locus ID | 93871 |
UniProt ID | Q921C3 |
Gene Summary | May be a transcriptional activator. May be involved in chromatin remodeling. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the control of cell shape.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |